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Hyemin Oh,Sejeong Kim,Soomin Lee,Heeyoung Lee,Jimyeong Ha,Jeeyeon Lee,Yukyung Choi,Kyoung-Hee Choi,Yohan Yoon 한국축산식품학회 2016 한국축산식품학회지 Vol.36 No.6
This study aimed to evaluate the prevalence of Listeria monocytogenes on livestock farms in Korea and determine their serotypes and genetic correlations. Twenty-five livestock farms in Korea (central: 15, south west: 7, south east: 3) were visited 2-3 times, and 2,018 samples (feces: 677, soil: 680, silage: 647, sludge: 14) were collected. Samples were enriched in LEB (Listeria enrichment broth) and Fraser broth media, and then plated on Palcam agar. The isolates were identified by PCR and 16S rRNA gene sequencing. Then, the serotypes, presence of virulence genes (actA, inlA, inlB, plcB, and hlyA), and antibiotic resistance were determined. Genetic correlations among the isolates were evaluated by analyzing the restriction digest pattern with AscI. Of the 2,018 samples, only 3 (0.15%) soil samples (FI-1-FI-3) from 1 farm in the south east region were positive for L. monocytogenes. Based on biochemical tests and multiplex PCR, the serotype of the isolates were 4ab (FI-1 and FI-3) and 3a (FI-2), which are not common in foodborne L. monocytogenes. The 3a serotype isolate was positive for all tested virulence genes, whereas the 4ab serotype isolates were only positive for hlyA, actA, and inlA. The isolates were resistant to all 12 tested antibiotics, especially FI-3. The genetic correlations among the isolates were 100% for those of the same serotype and 26.3% for those of different serotypes. These results indicate that the prevalence of L. monocytogenes on livestock farms in Korea is low; however, the isolates are pathogenic and antibiotic resistant.
Hyemin Oh,Dohyun Kwon,Jaemyung Ahn,Jun-Young Paeng 대한악안면성형재건외과학회 2022 Maxillofacial Plastic Reconstructive Surgery Vol.44 No.-
Background: Complications from osteoradionecrosis (ORN) and medication-related osteonecrosis of the jaw (MRONJ) include oro-cutaneous fistulas, necrotic bone exposure, soft-tissue defects, and pathologic fractures. The fibula free flap (FFF) is a common free flap method used to reconstruct the mandible in severe cases. Recently, we have used the FFF successfully for the reconstruction of ORN and MRONJ mandibular defects. We report this method as a recommended technique for the treatment of ORN and MRONJ and the management method of postoperative infections. Methods: Four patients who were diagnosed with ORN of the mandible and 3 patients who were diagnosed with MRONJ of the mandible were included in the study. Among the 7 patients, 3 patients also had pathologic fractures. Partial mandibulectomy and FFF reconstruction were performed at the Department of Oral and Maxillofacial Surgery, Samsung Medical Center from April 2019 to March 2021. Results: All 7 patients recovered following the reconstruction of the defect by FFF. Four patients experienced infections after surgery and pus cultures were performed. All were well healed without flap damage after changing the antibiotics by consultation with infectious medicine experts. Conclusion: FFF is a widely used method and can provide an extensive flap to reconstruct the mandible, especially those affected by ORN or MRONJ. If an infection occurs after surgery, appropriate antibiotic changes should be made through cooperation with the infectious medicine department. Therefore, FFF is a well-established and recommended method even in cases of challenging reconstruction.
Oh, Hyemin,Kim, Sejeong,Lee, Soomin,Lee, Heeyoung,Ha, Jimyeong,Lee, Jeeyeon,Choi, Yukyung,Choi, Kyoung-Hee,Yoon, Yohan Korean Society for Food Science of Animal Resource 2016 한국축산식품학회지 Vol.36 No.6
This study aimed to evaluate the prevalence of Listeria monocytogenes on livestock farms in Korea and determine their serotypes and genetic correlations. Twenty-five livestock farms in Korea (central: 15, south west: 7, south east: 3) were visited 2-3 times, and 2,018 samples (feces: 677, soil: 680, silage: 647, sludge: 14) were collected. Samples were enriched in LEB (Listeria enrichment broth) and Fraser broth media, and then plated on Palcam agar. The isolates were identified by PCR and 16S rRNA gene sequencing. Then, the sero-types, presence of virulence genes (actA, inlA, inlB, plcB, and hlyA), and antibiotic resistance were determined. Genetic correlations among the isolates were evaluated by analyzing the restriction digest pattern with AscI. Of the 2,018 samples, only 3 (0.15%) soil samples (FI-1-FI-3) from 1 farm in the south east region were positive for L. monocytogenes. Based on biochemical tests and multiplex PCR, the serotype of the isolates were 4ab (FI-1 and FI-3) and 3a (FI-2), which are not common in foodborne L. monocytogenes. The 3a sero-type isolate was positive for all tested virulence genes, whereas the 4ab serotype isolates were only positive for hlyA, actA, and inlA. The isolates were resistant to all 12 tested antibiotics, especially FI-3. The genetic correlations among the isolates were 100% for those of the same serotype and 26.3% for those of different serotypes. These results indicate that the prevalence of L. monocytogenes on livestock farms in Korea is low; however, the isolates are pathogenic and antibiotic resistant.
Oh, Hyemin,Yoon, Yohan,Ha, Jimyeong,Lee, Jeeyeon,Shin, Il-Shik,Kim, Young-Mog,Park, Kwon-Sam,Kim, Sejeong The Korean Society of Fisheries and Aquatic Scienc 2021 Fisheries and Aquatic Sciences Vol.24 No.6
This study evaluated the risk of foodborne illness from highly pathogenic Vibrio spp. (Vibrio vulnificus and V. cholerae) by raw whip-arm octopus (Octopus minor) consumption. In total 180 samples of raw whip-arm octopus were collected from markets and examined for the prevalence of V. vulnificus and V. cholerae. Predictive models describing the kinetic behavior of Vibrio spp. in raw whip-arm octopus were developed, and the data on amounts and frequency of raw whip-arm octopus consumption were collected. Using the collected data, a risk assessment simulation was conducted to estimate the probability of foodborne illness raw whip-arm octopus consumption using @RISK. Initial contamination levels of Vibrio spp. in raw whip-arm octopus were -3.9 Log colony-forming unit/g, as estimated by beta distribution fitting. The developed predictive models were appropriate to describe Vibrio spp. in raw whip-arm octopus during distribution and storage with R<sup>2</sup> values of 0.946-0.964. The consumption frequency and daily consumption amounts of raw whip-arm octopus per person were 0.47% and 57.65 g, respectively. The probability of foodborne illness from raw whip-arm octopus consumption was estimated to be 8.71 × 10<sup>-15</sup> for V. vulnificus and 7.08 × 10<sup>-13</sup> for V. cholerae. These results suggest that the risk of Vibrio spp. infection from raw whip-arm octopus consumption is low in South Korea.
Hyemin Oh,Sejeong Kim,Soomin Lee,Jimyeong Ha,Jeeyeon Lee,Yukyung Choi,Yewon Lee,Yujin Kim,Yeongeun Seo,Yohan Yoon 한국축산식품학회 2018 한국축산식품학회지 Vol.38 No.6
This study developed an antimicrobial hydrogel to control Listeria monocytogenes in Yukhoe (Korean beef tartare). Four hydrogels (hydrogel 1: 5% alginate+1% chitosan+0.2% CaCl2, hydrogel 2: 1% κ-carrageenan+1% chitosan, hydrogel 3: 2%κ-carrageenan+1% CaCl2, and hydrogel 4: 2% κ-carrageenan+3% CaCl2) were prepared. The hydrogels then absorbed 0.1% grapefruit seed extract (GSE) and 0.1% citrus extract (CE) for 30, 60, 120, and 240 min to be antimicrobial hydrogels. To select the most effective antimicrobial hydrogel, their swelling ratio (SR) and antilisterial activities were determined. The selected hydrogel (2×2 cm) was then placed on surface of beef (round; 3×3 cm), where L. monocytogenes (ca. 106 CFU/g) were inoculated, and the cell counts were enumerated on PALCAM agar. Among the hydrogels, the SR of hydrogel 1 increased with absorbing time, but other hydrogels showed no significant changes. Antimicrobial hydrogel 1 showed higher (p<0.05) antilisterial activity than other antimicrobial hydrogels, especially for the one absorbed the antimicrobial for 120 min. Thus, the antimicrobial hydrogel 1 absorbed antimicrobials for 120 min was applied on raw beef at 4℃, and reduced (p<0.05) more than 90% of L. monocytogenes on raw beef. These results indicate that antimicrobial hydrogel 1 formulated with 0.1% GSE or 0.1% CE is appropriate to improve the safety of Yukhoe by reducing psychrotrophic L. monocytogenes cell counts on raw beef.
중고등학생의 가정 및 학교에서 의견 청취 경험, 자아존중감이 인권 인식에 미치는 영향: 인권교육에 따른 차이 검증
오혜민(Hyemin Oh),김상원(Sangwon Kim),이양희(Yanghee Lee) 한국콘텐츠학회 2021 한국콘텐츠학회논문지 Vol.21 No.5
본 연구는 아동의 가정과 학교에서 의견 청취 경험, 자아존중감, 그리고 인권 인식 간 구조적 관계를 탐색하는데 그 목적이 있다. 본 연구에서는 연구가설을 검증하기 위해 2017년 아동 · 청소년 인권실태 조사의 중 · 고등학생 응답(6,405명, 여자 47.8%)을 대상으로 Mplus를 사용하여 구조방정식 모형을 분석하였다. 그 결과는 다음과 같다. 첫째, 가정 또는 학교에서 의견이 청취된 경험을 한 아동일수록 자아존중감과 인권 인식이 높은 것으로 나타났다. 또한 자아존중감과 인권 인식은 정적인 관계를 보였으며, 자아존중감은 가정과 학교 각각에서의 의견이 청취된 경험이 인권 인식으로 이어지는 경로를 유의미하게 매개하였다. 추가적으로 인권교육을 받았는지의 유무에 따라 각 경로에 차이가 있는지 살펴본 결과 유의미한 차이는 없었다. 이러한 연구 결과를 토대로 아동의 인권 인식 증진을 위해 가정이나 학교에서 아동의 의견이 청취되는 환경조성을 위한 실천적 방안을 논의하였다. This study aims to explore the structural relationships among experiences of being heard in home and school settings, self-esteem, and human rights awareness. Among the 2017 NYPI Cross-sectional Survey on the Rights of Youth & Children, 6,405 middle and high school students (47.8% females) were used to conduct a structural equation modeling using Mplus. It was found that children who experience being heard in the home or school display higher levels of self-esteem and awareness of human rights. It was also found that self-esteem is more likely to increase awareness of human rights. Self-esteem was also found to mediate the pathway between the experience of being heard at home or school and human rights awareness. In addition, there was no significant difference in path coefficients depending on whether they received human rights education. Practical implications for promoting home and school environments where childrens opinions can be heard were discussed.
Hana Kwon(Hana Kwon),Hyemin Oh(Hyemin Oh),Hyunjee Chae(Hyunjee Chae),Pattaraporn Thiangthae(Pattaraporn Thiangthae ),Kyoung-Jin Kang(Kyoung-Jin Kang) 대한미용의학회 2022 대한미용의학회지 Vol.6 No.2
Background: Filler injections are commonly used to soften deep nasolabial folds (NLF) by restoring volume in the depressed parts of fold. Recently, a combination technique using hyaluronic acid filler and polydioxanone (PDO) thread significantly improves deep NLF and induced changes in smile. Objective: This study retrospectively confirmed the effects of this combination therapy, focusing on smile changes. Methods: The NLF was divided into 3 parts: the lateral folded part (LFP), medial depressed part (MDP), and central creased part (CCP). Between November and December 2021, 14 patients underwent the combination treatment; of these, 11 were enrolled, excluding 3 who were not properly followed up. On average, 5.82±0.87 (right) and 5.73±0.47 (left) PDO mesh threads (6.0 cm length) were sequentially inserted into the LFP, MDP, and under the CCP of each NLF. Following this, an average of 1.82±0.71 ml (right) and 1.92±0.75 ml (left) of the filler was also sequentially injected into each NLF in a similar manner. They were mainly placed in the superficial and deep fat layers in the entire NLF and sub-malar areas. Smile index was measured during the fully expanded smile stage. The follow-up period was 3 months. The results were estimated using pre- and post-operative photographs. Results: Three months after the combination treatment, patients were pleased with the softening of their NLF. As indicated by the increased smile index (inter-commissural width/inter-labial gap), the old and expanded smile changed to younger and less expanded smile. The original smile type (eight and three patients with the commissure and cuspid types, respectively) remained unchanged. The contour changes in each smile varied according to the smile type. This could be explained by combination treatment’s NLF correction, which restricts facial expression muscle movement. Conclusion: These results demonstrate definite evidence of softening of the NLF, a youthful smile, and an increased smile index.
Cross-Border Spillover Effect of Particulate Matter Pollution between China and Korea
( Hyemin Park ),( Wonhyuk Lim ),( Hyungna Oh ) 한국경제학회 2020 The Korean Economic Review Vol.36 No.1
Given its adverse health effects, particulate matter (PM) pollution has become a critical public policy issue in Northeast Asia. As concerns about PM pollution increase, so does the interest in identifying its origins, including transboundary pollutant sources. Employing the daily average PM10 concentration level data from Beijing, Shanghai, and Seoul in 2014- 2016, we estimate the direction and extent of the spillover effect of the PM10 density between China and Korea. Estimation outcomes suggest that the PM10 density levels in Beijing and Shanghai are Granger causes of the PM density in Seoul, but not vice versa. That is, the PM 10 density in Seoul increased by 0.13 and 0.133 ppm in response to the 1 ppm increase in the PM10 density in Beijing and Shanghai on the previous day, respectively. The cross-border spillover effect from Beijing decreased by 0.076 ppm from May to October when the air flow hindered the PM10 sources generated in Beijing from reaching Seoul.