Novel anti-apoptotic mechanism of A20 through targeting ASK1 to suppress TNF-induced JNK activation

Won, M,Park, K A,Byun, H S,Sohn, K-C,Kim, Y-R,Jeon, J,Hong, J H,Park, J,Seok, J H,Kim, J M,Yoon, W-H,Jang, I-S,Shen, H M,Liu, Z G,Hur, G M Macmillan Publishers Limited 2010 CELL DEATH AND DIFFERENTIATION Vol.17 No.12

The zinc-finger protein A20 has crucial physiological functions as a dual inhibitor of nuclear factor-κB (NF-κB) activation and apoptosis in tumor necrosis factor (TNF) receptor 1 signaling pathway. Although the molecular basis for the anti-NF-κB function of A20 has been well elucidated, the anti-apoptotic function of A20 is largely unknown. Here, we report a novel mechanism underlying the anti-apoptotic function of A20: A20 blocks TNF-induced apoptosis through suppression of c-jun N-terminal kinase (JNK) by targeting apoptosis signal-regulating kinase1 (ASK1). First, the ectopic expression of A20 drastically inhibits TNF-induced JNK activation and apoptosis in multiple cell types including those deficient of NF-κB activation. Unexpectedly, the blunting effect of A20 on TNF-induced JNK activation is not mediated by affecting the TNFR1 signaling complex formation. Instead, A20 interacts with ASK1, an important MAPKK kinase in the JNK signaling cascade. More importantly, overexpression of wild-type A20, but not of mutant A20 (ZnF4; C624A, C627A), promotes degradation of the ASK1 through the ubiquitin-proteasome system. Taken together, the results from this study reveal a novel anti-apoptotic mechanism of A20 in TNF signaling pathway: A20 binds to ASK1 and mediates ASK1 degradation, leading to suppression of JNK activation and eventually blockage of apoptosis.

한국인에서 혈소판 당단백 Ⅱb/Ⅲa 유전자 다형성과 관동맥 성형술 후 재 협착과의 관계

이민수,이정우,김보영,임대승,강정아,김정희,김윤철,성보영,최성준,성인환,전은석 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2

Platelet aggregation is the final pathway of acute coronary syndrome such as acute myocardial infarction and unstable angina. Platelet glycoprotein IIb/IIIa is a membrane receptor for fibrinogen and yon Willebrand factor and it plays an important role in platelet aggregation and in the pathogenesis of acute coronary syndrome. It is known that polymorphism of the gene that encoding platelet glycoprotein IIb/IIIa(PI^A1/A2) is strongly related to acute coronary syndrome in Caucasian, but not in Koreans. We investigated relationship between platelet glycoprotein llb/Illa gene polymorphism and restenosis of coronary artery after angioplasty in Koreans. Total 371 patients(M=251. F=120) were enrolled. Angioplasty group comprised 143 patients who underwent coronary angioplasty, and in the angioplasty group, restenosis group comprised with the 65 patients who had restenotic lesion over 50% of luminal diameter in follow-up coronary angiography. Normal group comprised 153 patients who had no significant angiographic lesion and variant angina group comprised 75 patients who were positive in ergonovine test. Genomic DNA was extracted from peripheral arterial blood. To determine the frequency of P1^A1/A2 genotype, polymerase chain reaction(PCR) was done and the product was restricted with Mspl. 3%. agarrose gel electrophoresis showed restriction fragment length polymorphism. Clinical profile and risk factor were also reviewed. Among all 371 patients of study group, genotype of only one patients in restenosis group if is proven to be PI^A1/A2 heterozygote. All patients of normal study group, no restenosis group, and the other patients in restenosis group have an PI^A1 homozygote genotype. In our study, platelet glycoprotein IIb/Illa polymorphism has no relationship with restenosis of the coronary artery after angioplasty in Koreans. But the genotypic frequency of platelet glycoprotein IIb/IIIa gene polymorphism in Koreans is concordant with that of previous studies.

V994 Herculis: the multiple system with a quadruple-lined spectrum and a double eclipsing feature

Lee, C.-U.,Kim, S.-L.,Lee, J. W.,Kim, C.-H.,Jeon, Y.-B.,Kim, H.-I.,Yoon, J.-N.,Humphrey, A. Blackwell Publishing Ltd 2008 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.389 No.4

<P>ABSTRACT</P><P>We report the discovery of a multiple system with a quadruple-lined spectrum and a double eclipsing feature. Our photometric and high-resolution spectroscopic data show that V994 Herculis (V994 Her; ADS 11373 AB) is composed of two pairs of double-lined eclipsing binaries, which we designate as A and B. System A consists of a B8V+A0V binary with an orbital period of 2.083 264 d and system B of a A2V+A4V binary with 1.420 033 d. Our light curves show that both of them have a detached binary configuration. We derive masses and radii of four components (Aa, Ab, Ba and Bb) from the synthetic analyses of light curves and radial velocity curves. The masses of systems A and B are <I>M</I><SUB>Aa</SUB>= 2.83 ± 0.20 M<SUB>⊙</SUB>, <I>M</I><SUB>Ab</SUB>= 2.30 ± 0.16 M<SUB>⊙</SUB>, <I>M</I><SUB>Ba</SUB>= 1.87 ± 0.12 M<SUB>⊙</SUB> and <I>M</I><SUB>Bb</SUB>= 1.86 ± 0.12 M<SUB>⊙</SUB>, with radii <I>R</I><SUB>Aa</SUB>= 2.15 ± 0.05 R<SUB>⊙</SUB>, <I>R</I><SUB>Ab</SUB>= 1.71 ± 0.04 R<SUB>⊙</SUB>, <I>R</I><SUB>Ba</SUB>= 1.59 ± 0.08 R<SUB>⊙</SUB> and <I>R</I><SUB>Bb</SUB>= 1.50 ± 0.08 R<SUB>⊙</SUB>, respectively. These masses and radii are well consistent with the empirical relation for double-lined eclipsing binaries.</P>

관상동맥 스텐트 시술 후의 재협착에 관한 연구

김윤철,이정우,김보영,강정아,임대승,이민수,김정희,성보영,최성준,성인환,전은석 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.1

Coronary stent implacement is known as an effective treatment in the intimal dissection after percutaneous transluminal coronary angioplasty and the prevention of restenosis. However, In-stent restenosis still remains a major concern in clinical stenting. The stents were placed in 103 patients from July 1996 to March 1999 and performed follow-up coronary angiograms in 59(57.3%) patients. To identify the clinical, angiographic and procedurerelated variables 'which predict late restenosis within the stented artery, 59 patients(58.3±9.9, M:F= 41:18) were studied. The clinical characteristics of the patients were stable angina in 23(39.0%), unstable angina in 14(23.7%), acute myocardial infarction in 21(35.6%) and old myocardial infarction in 1(1.7%). Coronary stenting was performed in 1 patient(1.7%) for primary lesion, 50 patients(84.7%) for suboptimal results after PTCA, 6 patients(10.2%) for bail-out procedure, and 2 patients(3.4%) for restenotic lesions. All patients were treated with aspirin and ticlopidinc. The follow-up angiograms were obtained at 7±4 months. The overall in-stent restenosis rate was 27.1%. The coronary angiographic findings were 32 single vessel(54.2%), 19 two vessel(32.2%) and 8 three vessel disease(13.6%). The angiographic morphological characteristics were type A in 33(55.9%), type B in 14(23.7%), type C in 12(20. 3%) cases. Variables of 16 patients with restenosis were compared with those of 43 patients without restenosis. Previously known predictors for in-stent restenosis were multiple stenting, stenting for restenotic lesions, residual stenosis after stenting, stenting for total occlusion lesions, reference diameter, balloon to vessel ratio, acute gain and minimal luminal diameter after procedure, design and characteristics of stents, ostial lesion of aorta, high pressure method for stenting, lesion length, diabetes mellitus, size of artheroma, saphenous vein grafts, ulcerlating lesions and calcified lesions. In this study, Reference diameter before stenting(2.43±0.54mm vs. 2.88±0.65mm, p=0.016) and balloon-to-artery ratio(1.28±0.26 vs. 1.11±0.18, p=0.006) were predictors for in-stent restenosis. 1) The overall in-stent restenosis rate was 27.1%. 2) In the analysis of predictors for in-stent restenosis, there was no significant differences in clinical, angiographic factors between group with restenosis and without restenosis. But, Only reference diameter before stenting and balloon-toartery ratio were predictors of late in-stent restenosis. In conclusion, stenting is effective revascularisation method for selected patients with ischemic heart disease, and to minimize in-stent restenosis rate, stent implanting is achieved in a large vessel on the basis of an artery-to-stnet ration of 1:1, if possible.

ACTN-3 유전자 다형성과 무산소성 파워 능력

김철현 ( Chul Hyun Kim ),이영익 ( Young Ik Lee ),김윤만 ( Yoon Man Kim ),조인호 ( In Ho Cho ),제임스전 ( James Jeon ),김지연 ( Ji Yeon Kim ),박재현 ( Jae Hyun Park ),김혜진 ( Hea Jin Kim ),신경아 ( Kyung A Shin ),이승주 ( Seong Ju 한국운동영양학회 2004 Physical Activity and Nutrition (Phys Act Nutr) Vol.8 No.3

The α-actinin is an actin-binding protein belonging to the spectrin protein superfamily. a-actinin-3(ACTN-3) expression is limited to skeletal muscle, especially Type Ⅱ muscle fiber. This gene has homozygosity for a premature stop codon resulting in a-actinin-3 deficiency. The deficiency in the type Ⅱ muscle is able to be compensated by a-actinin-2(ACTN-2). While that deficiency does not induce a disease phenotype, the ACTN-3 is highly conserved in evolutionary terms because of its functions independent of the ACTN2. Researchers have suggested that this trait is related to muscle function at the extremes of power performance. Therefore, we compared the relative frequencies of the ACTN-3 R577X polymorphism between anaerobic power athletes and control group. For this study, we recruited 158 sprint or power elite athletes and 414 healthy adults. The results of the current study showed significant differences in the genotype frequencies such that elite anaerobic power athletes have 4% higher of the RR genotype and 9% lower of the XX genotype than the healthy adults. With respect to the allele frequencies, the athletic group had significantly higher R allele frequency and significantly lower X allele frequency than the control group. In summary, these results suggest that the ACTN-3 R577X genotype may represent a genetic marker for anaerobic power performance.

Simultaneous Detection of Glabridin, (−)-α-Bisabolol, and Ascorbyl Tetraisopalmitate in Whitening Cosmetic Creams Using HPLC-PAD

Jeon, Jong-Sup,Kim, Han-Taek,Kim, Myeong-Gil,Oh, Moon-Seog,Hong, Se-Ra,Yoon, Mi-Hye,Shin, Ho-Chul,Shim, Jae-Han,Afifi, Nehal Aly,Hacımü,ftü,oğ,lu, Ahmet,Abd El-Aty, A. M. Springer-Verlag 2016 Chromatographia Vol.79 No.13

<P>A simultaneous analytical method was developed and validated to quantify three lipophilic compounds; namely glabridin (an isoflavonoid isolated from crude licorice), (-)-alpha-bisabolol (a sesquiterpene alcohol obtained from plant extracts), and ascorbyl tetraisopalmitate (a fat-soluble molecule derived from vitamin C) in functional cosmetic cream using high-performance liquid chromatography (HPLC) coupled with photodiode array detection (PAD). Cosmetic cream samples were extracted with a mixture of acetonitrile and isopropyl alcohol (45:55, v/v) and the target compounds were separated on a C-18 column with a gradient mobile phase consisting of deionized water, acetonitrile, and isopropyl alcohol. The detector wavelengths were 228, 202, and 221 nm, for glabridin, (-)-alpha-bisabolol, and ascorbyl tetraisopalmitate, respectively. The calibration curves showed good linearity with determination coefficients (R (2)) a parts per thousand yen 0.999. The mean recoveries were ranged between 89.8 and 103.9 % with relative standard deviations (RSDs) < 5 %. The intra- and inter-day precision was < 2 %. The limits of detection (LODs) were 0.03, 0.4, and 4.02 mu g mL(-1) for glabridin, (-)-alpha-bisabolol, and ascorbyl tetraisopalmitate, respectively. The method was successfully applied for monitoring 11 market samples, in which glabridin was quantified in the range of 17.5-25 mg 100 g(-1), (-)-alpha-bisabolol in the range of 25.1-677 mg 100 g(-1), and 140.6-291.5 mg 100 g(-1) for ascorbyl tetraisopalmitate. The proposed analytical method is simple, sensitive, and versatile and can be used for the quantification of lipophilic compounds in cosmetics in a single chromatographic run.</P>

Control of the microstructure of SnS photovoltaic absorber using a seed layer and its impact on the solar cell performance

Kang, Jeong-yoon,Kwon, Soo-Min,Yang, So Hyun,Cha, Jung-Hwa,Bae, Jin A.,Jeon, Chan-Wook Elsevier 2017 Journal of alloys and compounds Vol.711 No.-

<P><B>Abstract</B></P> <P>SnS thin films were prepared by sulfurizing a sputtered metallic Sn precursor film deposited on a Mo-coated sodalime glass substrate. Some of the precursors were deposited on a thin e-beam evaporated SnS seed layer with a 100–300 nm thickness and the effects of the seed layer on the sulfurization reaction were investigated. The seed layer provided nucleation sites for SnS crystals during the sulfurization and promoted a (040) preferred orientation, whereas a strong (101) orientation was observed in the non-seeded SnS absorber films. In addition, with introduction of the seed layer, the columnar grains observed in the non-seeded SnS film were transformed to an equiaxed grain structure with many grain boundaries. The SnS thin films with (040) preferential orientation and equiaxed crystal structure contributed not only to improvement of solar cell performance parameters, but also to ensuring uniformity between samples. This is considered to be related to the three-dimensional arrangement of the interlayer existing in the SnS unit cell having a very long b axis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SnS obtained by sulfurization of Sn deposited on SnS seed layer. </LI> <LI> Strong (040) preferred orientation in seeded SnS absorber layer. </LI> <LI> Equiaxed SnS grans in seeded SnS in contrast to columnar grain in non-seeded SnS. </LI> <LI> Superior solar cell performance of seeded SnS absorber. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

DNA Polymorphisms in SREBF1 and FASN Genes Affect Fatty Acid Composition in Korean Cattle (Hanwoo)

Bhuiyan, M.S.A.,Yu, S.L.,Jeon, J.T.,Yoon, D.,Cho, Y.M.,Park, E.W.,Kim, N.K.,Kim, K.S.,Lee, J.H. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.6

Sterol regulatory element binding factor 1 (SREBF1) and fatty acid synthase (FASN) genes play an important role in the biosynthesis of fatty acids and cholesterol, and in lipid metabolism. This study used polymorphisms in the intron 5 of bovine SREBF1 and in the thioesterase (TE) domain of FASN genes to evaluate their associations with beef fatty acid composition. A previously identified 84-bp indel (L: insertion/long type and S: deletion/short type) of the SREBF1 gene in Korean cattle had significant associations with the concentration of stearic (C18:0), linoleic (C18:2) and polyunsaturated fatty acids (PUFA). The stearic acid concentration was 6.30% lower in the SS than the LL genotype (p<0.05), but the linoleic and PUFA contents were 11.06% and 12.20% higher in SS compared to LL (p<0.05). Based on the sequence analysis, five single nucleotide polymorphisms (SNPs) g.17924G>A, g.18043C>T, g.18440G>A, g.18529G>A and g.18663C>T in the TE domain of the FASN gene were identified among the different cattle breeds studied. Among these, only g.17924 G>A and g.18663C>T SNPs were segregating in the Hanwoo population. The g.17924G>A SNP is a non-synonymous mutation (thr2264ala) and was significantly associated with the contents of palmitic (C16:0) and oleic acid (C18:1). The oleic acid concentration was 3.18% and 2.79% higher in Hanwoo with the GG genotype than the AA and AG genotypes, respectively (p<0.05), whereas the GG genotype had 3.8% and 4.01% lower palmitic acid than in those cattle with genotype AA and AG, respectively (p<0.05). Tissue expression data showed that SREBFI and FASN genes were expressed in a variety of tissues though they were expressed preferentially in different muscle tissues. In conclusion, the 84-bp indel of SREBF1 and g.17924G>A SNP of the FASN gene can be used as DNA markers to select Hanwoo breeding stock for fatty acid composition.

Theoretical Design for the Production of Quinoa (Chenopodium quinoa Willd.) in a Closed Plant Factory

Jong Hyang Bae,Jirapa Austin,Yoon-A Jeon,Mi-Kyung Cha,Young-Yeol Cho 한국원예학회 2016 원예과학기술지 Vol.34 No.6

Quinoa (Chenopodium quinoa Willd.) is a grain crop with high nutritional value. The leaves and sprouts of quinoa can also be consumed either raw or cooked, providing considerably nutritional value as well as high antioxidant and anticancer activities. This study was carried out to obtain basic data to assist in the practical design of a plant factory with artificial lighting for the cultivation of quinoa as a leafy vegetable. We estimated the energy content of the quinoa and the electrical energy required to produce this crop. The yield was 1,000 plants per day, with a planting density and light intensity of 0.015 ㎡ (15 × 10 ㎝) and 200 μ㏖·m<SUP>-2</SUP>·s<SUP>-1</SUP>, respectively. The total number of plants, cultivation area, and electricity consumption were estimated to be 25,000, 375 ㎡, and 93,750 μ㏖·s<SUP>-1</SUP>, respectively. White fluorescent lamps were used at a power of 20.4 ㎾ from 1,857 fluorescent lamps (FL, 55 W), and the cost for electricity was approximately 1,820 dollars (exchange rate of $1 = 1,200 won) per month. For a daily harvest of 1,000 plants per day in a closed plant factory, the estimated light installation cost, total installation cost, and total production cost would be 15,473, 46,421, and 55,704 dollars, respectively. The calculated production cost per plant, including labor costs, would be 27 cents for the 25-day cultivation period, with a marketable ratio of 80%. Considering the annual total expenses, income, and depreciation costs, the selling price per plant was estimated to be approximately 56 cents.

Theoretical Design for the Production of Quinoa (Chenopodium quinoa Willd.) in a Closed Plant Factory

Bae, Jong Hyang,Austin, Jirapa,Jeon, Yoon-A,Cha, Mi-Kyung,Cho, Young-Yeol Korean Society of Horticultural Science 2016 원예과학기술지 Vol.34 No.6

Quinoa (Chenopodium quinoa Willd.) is a grain crop with high nutritional value. The leaves and sprouts of quinoa can also be consumed either raw or cooked, providing considerably nutritional value as well as high antioxidant and anticancer activities. This study was carried out to obtain basic data to assist in the practical design of a plant factory with artificial lighting for the cultivation of quinoa as a leafy vegetable. We estimated the energy content of the quinoa and the electrical energy required to produce this crop. The yield was 1,000 plants per day, with a planting density and light intensity of $0.015m^2$ ($15{\times}10cm$) and $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, respectively. The total number of plants, cultivation area, and electricity consumption were estimated to be 25,000, $375m^2$, and $93,750{\mu}mol{\cdot}s^{-1}$, respectively. White fluorescent lamps were used at a power of 20.4 kW from 1,857 fluorescent lamps (FL, 55 W), and the cost for electricity was approximately 1,820 dollars (exchange rate of $1 = 1,200 won) per month. For a daily harvest of 1,000 plants per day in a closed plant factory, the estimated light installation cost, total installation cost, and total production cost would be 15,473, 46,421, and 55,704 dollars, respectively. The calculated production cost per plant, including labor costs, would be 27 cents for the 25-day cultivation period, with a marketable ratio of 80%. Considering the annual total expenses, income, and depreciation costs, the selling price per plant was estimated to be approximately 56 cents.