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( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.
뇌졸중 위험인자를 가진 어지럼 환자에서의 확산강조 자기공명영상
강형구,윤유상,이진희,박인철,이경룡,정상원,구홍두,김승호 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4
Background: The management of vertiginous patients is a great challenge to emergency physicians. We evaluated the diagnostic value of a diffusion-weighted image(DWI) in differentiating central vertigo from the peripheral vertigo in patients who presented no neurological symptoms other than risk factors for stroke. Methods: From March 2000 to February 2001, we retrospectively analyzed the cases of 68 patients who visited the emergency department with symptoms of isolated vertigo and who had risk factors for stroke. DWIs, computed tomograms(CT), and medical records were reviewed, and the final diagnose, the DWIs and the CT readings, the risk factors for stroke, and the time it took waiting for a DWI or CT scan were analyzed. Results: Of the 68 patients, 21(30.8%) had central vertigo: 15 vertebrobasilar transient ischemic attacks(VB-TIA), 4 brain stem infarctions, 1 cerebellar infarction, and 1 cerebellopeduncular infarction. The DWI showed a 28.6% sensitivity, a 97.9% specificity, and an 85.7% positive predictive value in diagnosing central vertigo. It also had a 100% sensitivity in detecting infarctions. Conclusion: A DWI had a comparable sensitivity to MRI in detecting central vertigo and small, but potentially, lethal infarctions in our patient population. We recommend clinical application of DWI in the emergency department evaluation of isolated vertigo patients with risk factors for stroke.
Assessments of in Vivo and in Vitro Tests for Atopic Diseases
Yi, Jong Yuk,Kim, Jin Wou,Kim, Hyung Ok,Kim, Chung Won CATHOLIC MEDICAL CENTER 1987 Bulletin of the Clinical Research Institute Vol.15 No.1
In order to evaluate the diagnostic value of Phadiatop^� test in atopic diseases, we compared the results of Phadiatop^� test with those of prick test, total serum IgE measurement, allergen specific IgE test in atopic dermatitis, atopic respiratory diseases, atopic respiratory diseases with atopic dermatitis and control. An allergen for prick and specific IgE test was house dust mite, Dermatophagoides pteronyssinus. The results were as follows: 1. In atopic respiratory diseases with or without atopic dermatitis, both prick test and Phadiatop^� test showed higher sensitivities than other tests. 2. The sensitivities of all tests which we tried were relatively lower in atopic dermatitis than in other diseases. 3. The specificities of prick test, allergen specific IgE test and Phadiatop^� test were high, while that of total serum IgE measurement was relatively low (83.3%).
Chung, Ge-Hoon,Kim, Tae-Hyung,Shin, Hye-Won,Chae, Eun-Hee,Yi, Han-Ju,Moon, Hong-Sik,Kim, Hyun-Jin,Kim, Joong-Soo,Jung, Sung-Jun,Oh, Seog-Bae The Korean Society of Pharmacology 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.4
In this study, we determined mode of action of a novel carbamoyloxy arylalkanoyl arylpiperazine compound (SKL-NP) on hyperpolarization-activated cyclic nucleotide-gated (HCN) channel currents ($I_h$) that plays important roles in neuropathic pain. In small or medium-sized dorsal root ganglion (DRG) neurons (< $40{\mu}m$ in diameter) exhibiting tonic firing and prominent $I_h$, SKL-NP inhibited $I_h$ and spike firings in a concentration dependent manner ($IC_{50}=7.85{\mu}M$). SKL-NP-induced inhibition of $I_h$ was blocked by pretreatment of pertussis toxin (PTX) and N-ethylmaleimide (NEM) as well as 8-Br-cAMP, a membrane permeable cAMP analogue. These results suggest that SKL-NP modulates $I_h$ in indirect manner by the activation of a Gi-protein coupled receptor that decreases intracellular cAMP concentration. Taken together, SKL-NP has the inhibitory effect on HCN channel currents ($I_h$) in DRG neurons of rats.
Assessment of IgE Detecting Methods in Cord and Maternal Serum
Yi, Jong Yuk,Park, Chun Wook,Kim, Hyung Ok,Kim, Chung Won CATHOLIC MEDICAL CENTER 1985 Bulletin of the Clinical Research Institute Vol.13 No.1
The aim of the present work is to evaluate the most accurate technique for analysing low levels of serum IgE. Following results are obtained from experiments by PRIST, Overnight PRIST, PRIST using RAST anti-IgE tracer and Overnight PRIST using RAST anti-IgE tracer methods with cord serum and maternal serum of 21 individuals respectively. 1. There was no statistical significance in maternal serum IgE liter using above 4 methods. However, the higher level of IgE titer was found in PRIST method when IgE was over 190 IU/ml (5 cases) estimated by PRIST method and in Overnight PRIST using RAST anti-IgE tracer method when IgE was below 190 IU/ml (16 cases) (P<0.05). 2. In cord serum IgE titer, only Overnight PRIST using RAST anti-IgE tracer method showed statistical significance. 3. The most desirable correlation coefficient between maternal serum and cord serum IgE titer was estimated by Overnight PRIST using RAST anti-IgE tracer method than any other methods.
Chung Ha-Na,Yi Soon-Hyung The Korean Home Economics Association 2005 International Journal of Human Ecology Vol.6 No.2
The purpose of this study was to examine the effects of family-school relations on children's school adjustment with divorced mothers. Subcategories of the family-school relations were family participation in decision making, family help for schools, learning activities at home, school help for families, and school-home communication adopted from Epstein's parent involvement theory. Sub categories of children's school adjustment were delinquent behavior and academic achievement. The sample of this study included 3,367 children from first to fifth grade who lived either in a two-parent or one-parent home. Among them, 411 children with divorced mothers were analyzed. Independent t-test, Pearson's correlations, stepwise regression analysis were all conducted. Findings suggested that children with divorced mothers showed higher delinquency and lower academic achievement than children in intact families. Sub categories of family involvement and school involvement were correlated in divorced families. Children's delinquency was predicted by three of the family-school relation factors, which were school-home communication, family help for schools, and school help for families. Children's academic achievement was predicted by ail factors.
Yi, Jung-Yoon,Lee, Gi-An,Lee, Jeong-Ran,Lee, Myung-Chul,Kang, Man-Jung,Baek, Hyung-Jin,Kim, Chung-Kon The Plant Resources Society of Korea 2011 한국자원식물학회지 Vol.24 No.3
It is very crucial to evaluate the genetic diversity of peanut genetic resources for identification of peanut germplasm accessions and variety improvement. Cultivated peanut generally has two subspecies, hypogaea and fastigiata. In this study, we identified peanut into three plant types, virginia (var. hypogaea), spanish (var. vulgaris), and valencia (var. fastigiata). Former one belongs to ssp. hypogaea and latter two are involved in ssp. fastigiata. Twenty SSR markers were used to assess the genetic variation of three sets, hypogaea, vulgaris, and fastigiata, respectively. Out of variety-specific SSR primers tried in this study, ten pairs of SSR primers showed polymorphisms. Each accession could be identified by a specific set of polymorphic SSR primers, and allele number was evaluated among accessions, with an average of 6.7 in var. hypogaea and 5.4 in var. vulgaris and fastigiata. For evaluation of genetic diversity, gene diversity ranged from 0.336 to 0.844 and PIC (polymorphism information contents) ranged from 0.324 to 0.827 were investigated. Dendrograms based on genetic distances were constructed, which showed the existence of three different clusters. And these three different clusters might be associated with the genes involved in three plant types. The results also suggested that there were plentiful SSR polymorphisms among peanut germplasm accessions in RDA (Rural Development Administration, Korea) Genebank and SSRs might play an important role in evaluating peanut accessions and cultivar improvement.