Expression Study of a Recombinant Plasmid containing Dipeptidyl Peptidase-4 Gene in E. coli : A Plausible Application for Celiac Disease Patients to Digest Gluten

Yeonjae Lee,Ryan Kang,Jenna Kwon,Kyuhee Jo,Jungbin Im,Sangwook Jung,DongHyun Lee,Juhyeon Lee,Jeong-Sang Lee 한국인터넷방송통신학회 2018 Journal of Advanced Smart Convergence Vol.7 No.2

Celiac disease (CD) is an immune-mediated enteropathy of small intestine diagnosed in both childhood and adulthood. CD is caused by gluten, which produces gliadorphin during its digestion. The enzyme dipeptidyl peptidase-4 (DPP4) breaks gliadorphin down nevertheless the last tripeptide remains and eventually inhibits DPP4, thus slowing down its process. Therefore, the idea is to produce an additional DPP4 enzyme which is crucial. Consequently, the functional DPP4 gene was cloned into pCDNA3 intermediate (FLAG+DPP4) vector and finally a recombinant plasmid pSB1C3 (Andersons promoters+ FLAG+DPP4) was constructed using synthetic biology. Normally, a DPP4 inhibitor is used as a cure for diabetes. Another important concern was overexpression of DPP4, which might lead to diabetes, accordingly the work was also performed for the regulation of the DPP4 gene expression. In this regard, three types of Anderson promoters (strong, moderate and weak) were utilized to study the control overexpression. This is the first report of idealistic trial for control the exogenous DPP4 gene-expression by molecular biologic tools.

Expression Study of a Recombinant Plasmid containing Dipeptidyl Peptidase-4 Gene in E. coli: A Plausible Application for Celiac Disease Patients to Digest Gluten

Lee, Yeonjae,Kang, Ryan,Kwon, Jenna,Jo, Kyuhee,Im, Jungbin,Jung, Sangwook,Lee, DongHyun,Lee, Juhyeon,Lee, Jeong-Sang The Institute of Internet 2018 International journal of advanced smart convergenc Vol.7 No.2

Celiac disease (CD) is an immune-mediated enteropathy of small intestine diagnosed in both childhood and adulthood. CD is caused by gluten, which produces gliadorphin during its digestion. The enzyme dipeptidyl peptidase-4 (DPP4) breaks gliadorphin down nevertheless the last tripeptide remains and eventually inhibits DPP4, thus slowing down its process. Therefore, the idea is to produce an additional DPP4 enzyme which is crucial. Consequently, the functional DPP4 gene was cloned into pCDNA3 intermediate (FLAG+DPP4) vector and finally a recombinant plasmid pSB1C3 (Andersons promoters+FLAG+DPP4) was constructed using synthetic biology. Normally, a DPP4 inhibitor is used as a cure for diabetes. Another important concern was overexpression of DPP4, which might lead to diabetes, accordingly the work was also performed for the regulation of the DPP4 gene expression. In this regard, three types of Anderson promoters (strong, moderate and weak) were utilized to study the control overexpression. This is the first report of idealistic trial for control the exogenous DPP4 gene-expression by molecular biologic tools.

The Experiences of Health Care Workers during the COVID-19 Pandemic in Korea: a Qualitative Study

Lee Ju-Yeon,Lee Ji-yeon,Lee So Hee,Kim Jangrae,Park Hye Yoon,Kim Yeonjae,Kwon Ki Tae 대한의학회 2021 Journal of Korean medical science Vol.36 No.23

Background: During the coronavirus disease 2019 (COVID-19) pandemic, health care workers (HCWs) have faced multiple physical and psychological challenges while carrying out their duties. In this study, we examined the experiences of specific groups of HCWs during the pandemic. Methods: From 18 November to 30 December 2020, we conducted a qualitative study using semi-structured, face-to-face interviews with four groups of 14 HCWs in three cities (Seoul, Daegu, and Gwangju) in South Korea. The HCWs who participated in the focus groups included physicians, nurses, medical practitioners, and cleaning staff who directly or indirectly cared for patients during the COVID-19 epidemic. Interviews were transcribed verbatim and analyzed using the consensual qualitative research approach. Results: Our qualitative data analysis revealed four main domains: work-related struggles, personal life-related struggles, psychological stress, and health-related struggles. Health care providers were challenged by working in critical situations and were overwhelmed by heavy workloads, fear of infection, lifestyle changes, and psychological and physical struggles. Conclusion: Our findings could serve as a foundation for establishing health care systems and policies that help HCWs cope with occupational stress, thus increasing their ability to adapt to the ongoing COVID-19 pandemic.

Possibility of Decreasing Incidence of Human Immunodeficiency Virus Infection in Korea

Lee Minkyeong,Park Wan Beom,Kim Eu Suk,Kim Yeonjae,Park Sang-Won,Lee Eunyoung,Oh Myoung-don,Kim Nam Joong,Kim Hong Bin,Song Kyoung-Ho,Choe Pyoeng Gyun,Kang Chang Kyung,Lee Chan Mi,Choi Yunsang,Moon So 대한감염학회 2023 Infection and Chemotherapy Vol.55 No.4

Background: The number of newly diagnosed cases of human immunodeficiency virus (HIV) infection in Korea, which had increased until 2019, has markedly decreased since the coronavirus disease 2019 pandemic started. This study evaluated whether the decrease is due to a reduction in the incidence of HIV infection and/or delayed diagnosis during the pandemic. Materials and Methods: We reviewed the medical records of 587 newly diagnosed patients with HIV infection between February 2018 and January 2022 from four general hospitals, and their characteristics were compared between the prepandemic and pandemic periods. The lapse time from infection to diagnosis was estimated using an HIV modeling tool. Results: The estimated mean times to diagnosis were 5.68 years (95% confidence interval [CI]: 4.45 - 6.51 years) and 5.41 years (95% CI: 4.09 - 7.03 years) before and during the pandemic, respectively (P = 0.016). The proportion of patients with acquired immunodeficiency syndrome-defining illnesses, expected to visit hospitals regardless of the pandemic, decreased from 17.2% before the pandemic to 11.9% during the pandemic (P = 0.086). Conclusion: The decrease in the number of newly diagnosed cases of HIV infection in Korea might have resulted from an actual decrease in the incidence of HIV infection rather than a worsening of underdiagnosis or delayed diagnosis.

Expert Consensus on Measures to Promote Physical and Psychological Health among COVID-19-Related Healthcare Workers in Korea using Delphi Technique

Lee So Hee,Noh Jin-Won,Kim Yeonjae,Ryoo Hyun Wook,Park Kyung-Hwa,Park Kyung-Hwa,Lee Shinwon,Cheong Hae Suk,Heo Sang Taek,Hong Ki Jeong,Kim Kyoung-Beom,Kwon Ki Tae 대한감염학회 2022 Infection and Chemotherapy Vol.54 No.2

Background The coronavirus disease 2019 (COVID-19) pandemic has caused health problems and distress among healthcare workers (HCWs), so supportive measures to promote their health and relieve distress are needed. Materials and Methods We conducted two rounds of Delphi surveys with 20 COVID-19-related frontline healthcare professionals and public officials. The surveys evaluated means of supporting HCWs’ health by improving health care systems and working environments in terms of effectiveness and urgency. The validity of the measures was assessed by calculating the content validity ratio. Results The top-priority measures to support HCWs were “secure isolation units capable of treating severe cases” in the facility infrastructure category, “secure nursing staff dedicated for patients in the intensive care units” in the personnel infrastructure category, “improve communication between central office and frontline field” in the cooperation system category, “support personal protective equipment and infection control supplies” in the aid supplies category, and “realization of hazard pay” in the physical/mental health and compensation category. Conclusion There was consensus among the experts on the validity and priorities of policies in the facility, personnel, cooperation, supplies, and compensation categories regarding measures to promote COVID-19 related HCWs’ health. Background The coronavirus disease 2019 (COVID-19) pandemic has caused health problems and distress among healthcare workers (HCWs), so supportive measures to promote their health and relieve distress are needed. Materials and Methods We conducted two rounds of Delphi surveys with 20 COVID-19-related frontline healthcare professionals and public officials. The surveys evaluated means of supporting HCWs’ health by improving health care systems and working environments in terms of effectiveness and urgency. The validity of the measures was assessed by calculating the content validity ratio. Results The top-priority measures to support HCWs were “secure isolation units capable of treating severe cases” in the facility infrastructure category, “secure nursing staff dedicated for patients in the intensive care units” in the personnel infrastructure category, “improve communication between central office and frontline field” in the cooperation system category, “support personal protective equipment and infection control supplies” in the aid supplies category, and “realization of hazard pay” in the physical/mental health and compensation category. Conclusion There was consensus among the experts on the validity and priorities of policies in the facility, personnel, cooperation, supplies, and compensation categories regarding measures to promote COVID-19 related HCWs’ health.

여행 리뷰 분석 사이트

이연재(YeonJae Lee),이승희(Seunghee Lee),안병구(Beongku An) 한국통신학회 2020 한국통신학회 학술대회논문집 Vol.2020 No.11

중도 지적장애인의 다면적 평가를 위한 ICF Core Set 개발: 현장전문가 면담을 통한 항목 추출을 중심으로

이연재(Lee, Yeonjae),임혜경(Lim Hyegyeoung) 부산대학교 교육발전연구소 2019 교육혁신연구 Vol.29 No.1

연구목적: 본 연구의 목적은 중도 지적장애인의 다면적 평가를 위한 ICF Core Set을 개발하는 것으로 현장전문가와의 면담을 통해 4개 영역의 항목을 추출하는 데 있다. 연구방법: 연구 참여자는 중도 지적장애인을 5년 이상 지도한 경험이 있는 현장전문가 10명이다. 면담 기간은 2017년 9월에서 11월까지 약 2개월이 소요되었다. 면담 질문지는 WHO(2001)가 공표한 ICF 분류체계의 신체기능 및 신체구조, 활동과 참여, 환경요인의 4개 영역에 대한 중도 지적장애인의 특성, 능력과 수행 정도, 지원의 정도를 알아보는 내용으로 구성하였다. 면담자료는 연구 참여자의 동의를 구해 모두 녹음한 후 필사본으로 전사한 다음 4개 영역으로 유형화하였다. 연구결과: 중도 지적장애인의 다면적 평가를 위한 ICF Core Set 개발에 포함되어야 할 항목으로 총 149개가 추출되었다. 영역별로는 신체기능이 57개 항목, 신체구조는 11개 항목, 활동과 참여는 51개 항목, 환경요인은 30개 항목이 각각 추출되었다. 논의 및 결론: 향후 본 연구에서 추출한 항목의 중요도를 평정하기 위하여 2차에 걸친 델파이 조사를 실시하여 중도 지적장애인의 다면적 평가를 위한 포괄형 및 단축형 ICF Core Set을 개발할 것이다. Purpose: The purpose of this study was to identify evaluation items through interviews with field experts in order to develop ICF Core Set for multidisciplinary evaluation of persons with severe intellectual disabilities. Method: The study participants were 10 field experts with more than five years of experience instructing persons with severe intellectual disabilities. The interview period took about two months from September to November 2017. The interview questionnaire was designed to understand the abilities and performance of persons with severe intellectual disabilities in the 4 areas(body function and structure, activities and participation, environmental factors, contextual factors) of the ICF system suggested by WHO(2001). The interview data were recorded and transferred to the transcripts with the consent of study participants, and the data were categorized into 4 areas under triangulation method. Results: A total of 149 items were extracted to be included in the development of the ICF Core Set for the multidisciplinary evaluation of persons with severe intellectual disabilities. There were 57 items on body functions, 11 items on body structures, 51 items on activities and participation, 30 items on environmental factors. Conclusion: Hereafter, a two-phase Delphi survey will be conducted to assess the importance of items extracted from this study, while developing a comprehensive and shortened version of ICF Core Set for multidisciplinary evaluation of persons with severe intellectual disabilities.

질적 지표를 활용한 국내 학습장애 및 학습부진학생 대상 어휘 중재 연구 동향 분석

이연재(Yeonjae Lee),채창희(Changhee Chae),김희은(Heeeun Kim),김동일(Dongil Kim) 한국특수교육교과교육학회 2022 특수교육교과교육연구 Vol.15 No.2

본 연구는 동향 분석 및 질적 지표 분석을 통해 학습장애 및 학습부진학생을 대상으로 한 국내 어휘 중재 연구가 ‘증거기반의 실제’로 여겨질 수 있는가를 고찰하는데 목적이 있다. 분석논문 14편을 대상으로 연구 동향의 일반적인 특성을 분석한 후, CEC(2014) 질적 지표에 얼마나 부합하는지 점수를 산출하였다. 학습장애 및 학습부진학생을 위한 어휘 중재 연구는 2002년에 처음 출간된 후 2021년까지 꾸준히 게재되고 있었고, 초등학생을 대상으로 한 연구가 대부분이었다. 중재 방법은 어휘획득전략 지도가, 중재 목적은 어휘력 향상이 가장 많았다. CEC 질적 지표에 따라 평정하였을 때, (준)집단설계연구는 권고지표 4가지를 포함하였을 때 49.6%, 포함하지 않았을 때 62.0%로 양호한 수준으로 지표를 충족하였다. 단일대상연구는 권고지표를 포함 여부와 무관하게 77.3%로 상당히 높게 CEC 질적지표를 충족하였다. 연구 결과에 기반하여 후속 어휘 중재 연구를 위한 시사점을 논의하였다. The purpose of this study was to examine whether Korean vocabulary intervention studies targeting students with learning disabilities and difficulties can be regarded as 'evidence-based practice' through research trend analysis and qualitative indicator analysis. After analyzing the general characteristics of the research trend for 14 studies, the score was calculated based on CEC (2014) quality indicators. Vocabulary intervention studies for students with learning disabilities and difficulties have been steadily published, and most of the studies were conducted on elementary school students. The most common intervention method was vocabulary acquisition strategy, and the purpose of intervention was improvement of vocabulary. The quality indicator satisfaction ratio of quasi-group-design study was 49.6%. The single-subject study showed relatively higher quality indicator satisfaction ratio of 77.3%. Based on the research results, implications for subsequent vocabulary intervention studies were discussed.

금융 신상품 개발을 위한 IT구현 프로젝트관리 특성에 관한 연구

이연재 ( Yeonjae Lee ),이희조 ( Heejo Lee ),이석주 ( Seoukjoo Lee ) 한국정보처리학회 2014 한국정보처리학회 학술대회논문집 Vol.21 No.1

은행의 경영환경은 급변하고 금융시장에서의 경쟁은 심화되고 있는 가운데 은행의 경쟁력 확보는 상품의 시장 진입 속도에 달려있으나, 상품 개발의 지연으로 시장 점유율의 확대 기회를 놓치고 있는 실정이다. 이에 국내 은행들은 시장 선점을 위해 IT기반의 금융 신상품 개발에 많은 투자를 하고 있다. 본 연구는 신속한 금융 신상품 개발을 위해 상품 조건들을 레고 블록처럼 조립하면 쉽고 빠르게 상품이 만들어 지는 IT기반의 상품 팩토리의 아키텍처와 상품 생성 관계도를 정의하며, 변동금리와 층화(Tier)계층의 상품 생성에 효율적인 매트릭스 조건의 이율형을 제시한다. 아울러 이러한 기반을 활용한 시스템의 개발 생산성과 프로젝트의 특성 및 효과 등을 검증한다.

FMEA 와 FTA 를 활용한 챗봇 시스템의 사용성 개선 프로세스

이연재 ( Yeonjae Lee ),송재우 ( Jaewoo Song ),한혁수 ( Hyuksoo Han ) 한국정보처리학회 2020 한국정보처리학회 학술대회논문집 Vol.27 No.2

챗봇(Chatbot)은 자연어처리기술 등 인공지능 기술을 기반으로 한 사용자 친화적인 대화 방식 인터페이스를 제공하는 장점이 있어, 금융, 상담, 주문 등 다양한 산업 분야에서 적용되고 있다. 그러나, 챗봇의 응답이 사용자의 정신 모형과 불일치하는 경우, 다음 대화를 이어가는데 어려움을 야기하게 된다. 그러므로, 챗봇의 사용성을 확보하기 위해서는 응답 오류의 제거 또는 완화가 필수적이다. 기존의 챗봇의 사용성 개선과 관련된 연구들은 설문조사와 인터뷰 등 사용성 평가를 통해 상위 수준의 개선 방향만을 제안하고 있다. 따라서, 챗봇 개발 시, 실무자들이 응답 오류의 문제점을 분석하고, 이를 해결하기 위한 구체적인 개선 방안을 제시하는 데 한계가 있었다. 본 논문에서는 FMEA(Failure Modes and Effects Analysis) 기법을 활용해, 응답 오류의 치명도를 파악하고, 치명적인 오류들에 대해서는 FTA(Fault Tree Analysis) 기법을 기반으로 원인 분석을 실시하여 구체적으로 문제를 해결하기 위한 프로세스를 제안한다. 본 프로세스의 효용성을 검증하기 위해 주문 도메인의 챗봇에 적용해 보았다.