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Huang Yan,Xu Yongqiang,Feng Siyin,He Pan,Sheng Bing,Ni Jiangdong 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
Bone marrow mesenchymal stem cell (BMSC)-derived exosomes have been found to enhance fracture healing. In addition, microRNAs contributing to the healing of various bone fractures have attracted widespread attention in recent years, but knowledge of the mechanisms by which they act is still very limited. In this study, we clarified the function of altered microRNA-19b (miR-19b) expression in BMSCs in fracture healing. We modulated miR-19b expression via mimics/inhibitors in BMSCs and via agomirs in mice to explore the effects of these changes on osteogenic factors, bone cell mineralization and the healing status of modeled fractures. Through gain- and loss-of function assays, the binding affinity between miR-19b and WWP1/Smurf2 was identified and characterized to explain the underlying mechanism involving the KLF5/β-catenin signaling pathway. miR-19b promoted the differentiation of human BMSCs into osteoblasts by targeting WWP1 and Smurf2. Overexpression of WWP1 or Smurf2 degraded the target protein KLF5 in BMSCs through ubiquitination to inhibit fracture healing. KLF5 knockdown delayed fracture healing by modulating the Wnt/β-catenin signaling pathway. Furthermore, miR-19b enhanced fracture healing via the KLF5/β-catenin signaling pathway by targeting WWP1 or Smurf2. Moreover, miR-19b was found to be enriched in BMSC-derived exosomes, and treatment with exosomes promoted fracture healing in vivo. Collectively, these results indicate that mesenchymal stem cell-derived exosomal miR-19b represses the expression of WWP1 or Smurf2 and elevates KLF5 expression through the Wnt/β-catenin signaling pathway, thereby facilitating fracture healing.
Ye, Ni,Wang, Bin,Quan, Zi-Fang,Pan, Hai-Bo,Zhang, Man-Li,Yan, Qi-Gui Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3
MicroRNA expression is a research focus in studies of tumors. This article concentrates attention on potential links between tumors caused by mouse mammary tumor virus (MMTV) and human breast cancer, in order to provide theoretical basis for using mouse model to search for miRNA effects mediated by Wnt/beta-catenin signaling in human breast cancer. By analyzing interactions between miRNAs and the Wnt/beta-catenin signaling pathway in breast cancer, we hope to casts light on more biological functions of miRNAs in the process of tumor formation and growth and to explore their potential value in cancer diagnosis, prognosis and treatment. Our endeavor aimed at providing theoretical basis for finding safer, more effective methods for treatment of human breast cancer at the miRNA molecular level.
Crushing Characteristics on Square Tubes Under Progressive Buckling
Kaibo Yan,Sisi Lu,Pan Wang,Weitao Ni,Zhaowei Chen,Shuen Zhao,Shuguang Yao 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.1
This paper presented the research on crushing characteristics for progressive bucking square tubes under axial loading using the dimensional analysis method. According to the mechanical properties of mild steel and aluminium alloy, the finite element models of progressive buckling square tubes made up of different materials were established. Then dimensional analysis was developed to obtain the influence of inherent parameters on energy absorption characteristics of progressive buckling square tubes, and a theoretical prediction model was proposed to predict the energy absorption characteristics of progressive buckling square tubes subjected to axial loading. The theoretical prediction results were found to match well with finite element analysis and dynamic test results. Furthermore, the equivalence methods of square tubes made up of different materials and geometric parameters were discussed. The results showed that the equivalence of deformation displacement and equivalence of mean crushing force are better equivalence methods for lightweight design, and keeping a constant length and width to obtain an equivalent thickness for progressive buckling square tubes is a more stable equivalence method.
Li‐Na Pan,Feng-ZhuWANG,Xin-Yue ZHANG,Yan-Ni ZHAO,Geng-Ping ZHU,Min LI 한국곤충학회 2018 Entomological Research Vol.48 No.3
Heat shock proteins (HSPs) are known to be induced in response to various stress factors. Although HSPs have been studied in a number of insects, not much is known about HSPs in the natural enemies of insects, especially parasitoids. In this study, we identified and characterized five full‐length HSP genes (Cchsp40, Cchsp60, Cchsp70, Cchsp83, and Cchsp90) from an endoparasitic chalcid wasp, Chouioia cunea, which parasitizes the fall webworm, Hyphantria cunea pupae, a worldwide pest. The expression of Cchsps in response to temperature, pesticide stresses and UV radiation were also investigated by quantitative real‐time polymerase chain reaction (RT‐qPCR). The results showed that all five Cchsps were induced in response to hot and cold temperatures. Four pesticides induced the abundant expression of Cchsp70, Cchsp83 and Cchsp90 while ultraviolet radiation up‐regulated Cchsp40, Cchsp70, Cchsp83 and Cchsp90. These results indicate the different transcriptional profiles of the five different Cchsps in response to various abiotic stresses. The findings of this study provide insights into the response of C. cunea to abiotic stresses and insight into the use of this parasitoid in biological control strategies.
Ming-hui Pan,Wen-cheng Tang,Yan Xing,Jun Ni 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.3
In order to reduce the deformation and assembly error in an antenna thin wall parts assembly process, the assembly location or clamping positions need to be optimized. In this paper, the effect of clamping positions and clamping force on the thin wall parts deformation is analyzed considering the gravity by finite element simulation under different clamping force. The clamping positions are optimized with the Adaptive Simulated Annealing (ASA), Multi-Island Genetic Algorithm (MIGA) and Particle Swarm Optimization (PSO) algorithm by Isight software to reduce the deformation. Comparing with MIGA and PSO method, the results show that the clamping position optimization based on ASA exhibits the better results and superior than the MIGA and PSO no matter whether it has the effect of gravity. Simultaneously, through establishing the contact force model and contact deformation model, the nonlinear relationship between contact force, contact deformation and contact area is qualitatively analyzed, and the node deformation is calculated by theoretical formula. Comparing with those results, it is shown that the theoretical value matches well with the simulation results. Finally, some clamping scheme principles are proposed so that it provides a basis for reducing the deformation and assembly error of thin wall parts in future.