The Clamping Position Optimization and Deformation Analysis for an Antenna Thin Wall Parts Assembly with ASA, MIGA and PSO Algorithm

Ming-hui Pan,Wen-cheng Tang,Yan Xing,Jun Ni 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.3

In order to reduce the deformation and assembly error in an antenna thin wall parts assembly process, the assembly location or clamping positions need to be optimized. In this paper, the effect of clamping positions and clamping force on the thin wall parts deformation is analyzed considering the gravity by finite element simulation under different clamping force. The clamping positions are optimized with the Adaptive Simulated Annealing (ASA), Multi-Island Genetic Algorithm (MIGA) and Particle Swarm Optimization (PSO) algorithm by Isight software to reduce the deformation. Comparing with MIGA and PSO method, the results show that the clamping position optimization based on ASA exhibits the better results and superior than the MIGA and PSO no matter whether it has the effect of gravity. Simultaneously, through establishing the contact force model and contact deformation model, the nonlinear relationship between contact force, contact deformation and contact area is qualitatively analyzed, and the node deformation is calculated by theoretical formula. Comparing with those results, it is shown that the theoretical value matches well with the simulation results. Finally, some clamping scheme principles are proposed so that it provides a basis for reducing the deformation and assembly error of thin wall parts in future.

Contributed Mini Review : New paradigms on siRNA local application

( Meng Pan ),( Jin Wen Ni ),( Hui Ming He ),( Shan Gao ),( Xiao Hong Duan ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.3

Small interfering RNA (siRNA) functions through pairing with specific mRNA sequences and results in the mRNA`s degradation. It is a potential therapeutic approach for many diseases caused by altered gene expression. The delivery of siRNA is still a major problem due to its rapid degradation in the circulation. Various strategies have been proposed to help with the cellular uptake of siRNA and short or small hairpin RNA (shRNA). Here, we reviewed recently published data regarding local applications of siRNA. Compared with systemic delivery methods, local delivery of siRNA/shRNA has many advantages, such as targeting the specific tissues or organs, mimicking a gene knockout effect, or developing certain diseases models. The eye, brain, and tumor tissues are ‘hot’ target tissues/ organs for local siRNA delivery. The siRNA can be delivered locally, in naked form, with chemical modifications, or in formulations with viral or non-viral vectors, such as liposomes and nanoparticles. This review provides a comprehensive overview of RNAi local administration and potential future applications in clinical treatment. [BMB Reports 2015; 48(3): 147-152]

Evolution of Multi-temporal Landslides at Luhu, Miaoli County, Taiwan

( Che-ming Yang ),( Keng-hao Kang ),( Kuo-wei Li ),( Hui-jung Wang ),( Ching-ping Hsiao ),( Jyh-jong Liao ),( Yii-wen Pan ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2

A deep-seated landslide took place in Luhu, Miao-Li, Taiwan during intermittent rainfall in April 2018; initially, there were successive rock falls and gully erosion then leaded to a deep-seated landslide failure eventually. This study aims to understand the failure mechanism and the process of deep-seated failure. The study site is a steep anaclinal slope consisting of the Miocence Shihti Formation with thick sandstone, interbedded of sandstone and shale. We collected multi-temporal ortho-images and digital elevation (surface) models from 1980 to 2019 for geological investigation, geomorphological interpretation and numerical simulation. The images reveal several slope failures at 1998, 2003, 2005, 2009, 2015, 2017 and 2018 with various volume and failure types. The study area contains three sub-regions: the north, the northwest and the southwest slopes. The slope failure occurred repeatedly inside the north and the northwest slopes in the early stage. Gully erosion in the southwest slope progressed to a landslide on April 5, 2018 first and expanded to cover the deep-seated landslide failure in the northwest slope eventually; the slope failure resulted in a natural dam. After the landslide, several open cracks appeared near to the crown. The estimated maximum thickness, area and volume of the deep-seated landslide are ~60 m, 65,000 m2 and ~2 Mm3, respectively. Based on these results, we also performed a series of numerical simulation using the software RAMMS to interpret the run out and the deposition of this landslide case.

Identification and Characterization of the Antifungal Substances of a Novel Streptomyces cavourensis NA4s

( Hua Qi Pan ),( Su Ya Yu ),( Chun Feng Song ),( Nan Wang ),( Hui Ming Hua ),( Jiang Chun Hu ),( Shu Jin Wang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.3

A new actinomycete strain NA4 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antifungal activities against soilborne fungal pathogens. It was identified as Streptomyces cavourensis by morphological, physiological, and phylogenetic analyses based on its 16S rRNA gene sequence. The main antifungal components were isolated and identified from the fermentation culture as bafilomycins B1 and C1. These compounds exhibited significant antifungal activities and a broad antifungal spectrum. The results suggest that the Streptomyces cavourensis NA4 and bafilomycins B1 and C1 could be used as potential biocontrol agents for soilborne fungal diseases of plants.

Human Papillomavirus Vaccine Awareness, Acceptability, and Decision-Making Factors among Chinese College Students

Wang, Shao-Ming,Zhang, Shao-Kai,Pan, Xiong-Fei,Ren, Ze-Fang,Yang, Chun-Xia,Wang, Zeng-Zhen,Gao, Xiao-Hong,Li, Man,Zheng, Quan-Qing,Ma, Wei,Zhao, Fang-Hui,Qiao, You-Lin,Sivasubramaniam, Priya Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Background: College students are recommended as the target groups for catch-up human papillomavirus (HPV) vaccination. Systematical exploration of awareness, acceptability, and decision-making factors of HPV vaccination among Chinese college students has been limited. Materials and Methods: A multi-center survey was conducted in mainland China between November 2011 and May 2012. College students aged 18-22 years were stratified by their grade, gender, and major for sampling. Socio-demographic and HPV-related information such as knowledge, perceptions, acceptability, and attitudes were collected through a questionnaire. Results: A total of 3,497 undergraduates completed the questionnaire, among which 1,686 were males. The acceptability of the HPV vaccine was high (70.8%). Undergraduates from high-level universities, at lower grade, or with greater prior knowledge of HPV vaccines showed higher acceptability of HPV vaccination ($p_{trend}$ <0.001). Additionally, undergraduates with vaccination experience outside the National Expanded Program on Immunization (OR=1.29; 95%CI: 1.10-1.51) or fear of HPV-related diseases (OR=2.79; 95%CI: 2.28-3.41) were more willing to accept HPV vaccination. General knowledge of HPV vaccine was low among undergraduates, and safety was a major concern (71.05%). The majority of students wished to pay less than 300RMB for HPV vaccine and chose the Chinese Center for Disease Control and Prevention as the most appropriate venue for vaccination. Conclusions: Although most undergraduates demonstrate positive attitudes towards HPV vaccination, challenges pertaining to introduction exist in China. Corresponding proactive education and governmental subsidy to do so are urgently needed by this age-group population. Suggestions and potential strategies indicated may help shape the future HPV vaccination program in China.

A comparative experimental study on the cross-plane thermal conductivities of nano-constructed Sb2Te3/(Cu, Ag, Au, Pt) thermoelectric multilayer thin films

Yang Gang,Pan Jiahui,Fu Xuecheng,Hu Zhiyu,Wang Ying,Wu Zhimao,Mu Erzhen,Yan Xue-Jun,Lu Ming-Hui 나노기술연구협의회 2018 Nano Convergence Vol.5 No.22

Thermoelectric multilayer thin films used in nanoscale energy conversion have been receiving increasing attention in both academic research and industrial applications. Thermal transport across multilayer interface plays a key role in improving thermoelectric conversion efficiency. In this study, the cross-plane thermal conductivities of nano-constructed Sb2Te3/(Cu, Ag, Au, Pt) thermoelectric multilayer thin films have been measured using time-domain thermoreflectance method. The interface morphology features of multilayer thin film samples were characterized by using scanning and transmission electron microscopes. The effects of interface microstructure on the cross-plane thermal conductivities of the multilayer thin films have been extensively examined and the thermal transfer mechanism has been explored. The results indicated that electron–phonon coupling occurred at the semiconductor/metal interface that strongly affected the cross-plane thermal conductivity. By appropriately optimizing the period thickness of the metal layer, the cross-plane thermal conductivity can be effectively reduced, thereby improving the thermoelectric conversion efficiency. This work presents both experimental and theoretical understanding of the thermal transport properties of Sb2Te3/metal multilayer thin film junctions with important implications for exploring a novel approach to improving the thermoelectric conversion efficiency. Introduction Thermoelectric multilayer thin films used in nanoscale energy conversion have been receiving increasing attention in both academic research and industrial applications. Thermal transport across multilayer interface plays a key role in improving thermoelectric conversion efficiency. In this study, the cross-plane thermal conductivities of nano-constructed Sb2Te3/(Cu, Ag, Au, Pt) thermoelectric multilayer thin films have been measured using time-domain thermoreflectance method. The interface morphology features of multilayer thin film samples were characterized by using scanning and transmission electron microscopes. The effects of interface microstructure on the cross-plane thermal conductivities of the multilayer thin films have been extensively examined and the thermal transfer mechanism has been explored. The results indicated that electron–phonon coupling occurred at the semiconductor/metal interface that strongly affected the cross-plane thermal conductivity. By appropriately optimizing the period thickness of the metal layer, the cross-plane thermal conductivity can be effectively reduced, thereby improving the thermoelectric conversion efficiency. This work presents both experimental and theoretical understanding of the thermal transport properties of Sb2Te3/metal multilayer thin film junctions with important implications for exploring a novel approach to improving the thermoelectric conversion efficiency. Introduction

Suppression of CDK2 expression by siRNA induces cell cycle arrest and cell proliferation inhibition in human cancer cells

( Xiang E Long ),( Zhao Hui Gong ),( Lin Pan ),( Zhi Wei Zhong ),( Yan Ping Le ),( Qiong Liu ),( Jun Ming Guo ),( Jiu Chang Zhong ) 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.4

Cyclin-dependent kinase 2 (CDK2) is a member of serine/threonine protein kinases, which initiates the principal transitions of the eukaryotic cell cycle and is a promising target for cancer therapy. The present study was designed to inhibit cdk2 gene expression to induce cell cycle arrest and cell proliferation suppression. Here, we constructed a series of RNA interference (RNAi) plasmids which can successfully express small interference RNA (siRNA) in the transfected human cells. The results showed that the RNAi plasmids containing the coding sequences for siRNAs down-regulated the cdk2 gene expression in human cancer cells at the mRNA and the protein levels. Furthermore, we found that the cell cycle was arrested at G0G1 phases and the cell proliferation was inhibited by different siRNAs. These results demonstrate that suppression of CDK2 activity by RNAi may be an effective strategy for gene therapy in human cancers. [BMB reports 2010; 43(4): 291-296]

Preparation of Copper Sulfide by Microwave Assisted Method and Its Application on Superhydrophobic, Anti-UV and Photocatalytic Cotton Fabric

Ke-Cheng Yang,Li-Hui Xu,Hong Pan,Li-Ming Wang,Yong Shen,Ying Ding 한국섬유공학회 2020 Fibers and polymers Vol.21 No.6

In this work, solid spherical and hollow spherical CuS were prepared by microwave assisted method withPEG4000 as dispersant. The CuS was characterized by particle size analysis, scanning electron microscope(SEM), nitrogenadsorption and desorption, and X-ray diffraction (XRD), respectively. The effects of PEG4000, ratio of thiourea: copper, andmicrowave power on particle size of CuS were discussed, and the effects of particle size on photocatalytic property wereevaluated. The solid spherical CuS had average particle size of 189.1 nm and specific surface of 9.54 m2/g. The hollowspherical CuS had average particle size of 502.7 nm and specific surface area of 16.01 m2/g. The results showed the hollowspherical CuS had better photocatalytic property. In addition, the cotton fabric was treated by hollow spherical CuS/polydimethylsiloxane (PDMS) to prepare multifunctional textile in this work. The treated cotton fabric was characterized bySEM. The treated cotton fabric achieved the superhydrophobicity with contact angle of 156.9 o and sliding angle of 8 o, goodphotocatalytic properties for methylene blue, and exceptional UV resistance with UPF of 223.52.

Preparation of CuS/SiO2 Composite Modified Aerogel and Its Superoleophobic and Photocatalytic Properties

Ke-Cheng Yang,Li-Hui Xu,Hong Pan,Li-Ming Wang,Yong Shen,Ying Ding 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2020 NANO Vol.15 No.03

In this study, CuS/SiO2 composite modified aerogel was prepared by the incorporation of hollow spherical CuS into methyltrimethoxysilane-based SiO2 sol and modification with hexadeca-fluorodecyltriethoxysilane via acid-base catalyzed sol–gel reaction and drying under ambient pressure. The CuS/SiO2 composite modified aerogel was characterized by Fourier-transform infrared (FT-IR) spectrometry, scanning electron microscope (SEM), nitrogen gas adsorption and desorption and X-ray diffraction (XRD), respectively. The effects of CuS and fluorosilane concentration on density and porosity of aerogel, oleophobic and photocatalytic properties were evaluated. The results showed that structure and physical properties of aerogel had some effect by introducing CuS and fluorosilane, and the CuS/SiO2 composite modified aerogel with density of 0.146 g/cm3 and specific surface area of 241 m2/g achieved super-oleophobicity with oil contact angle of 152.8fl and sliding angle of 10fl, and good photocatalytic properties for methylene blue.

Simultaneous treatment with sorafenib and glucose restriction inhibits hepatocellular carcinoma in vitro and in vivo by impairing SIAH1-mediated mitophagy

Zhou Jing,Feng Ji,Wu Yong,Dai Hui-Qi,Zhu Guang-Zhi,Chen Pan-Hong,Wang Li-Ming,Lu Guang,Liao Xi-Wen,Lu Pei-Zhi,Su Wen-Jing,Hooi Shing Chuan,Ye Xin-Pin,Shen Han-Ming,Peng Tao,Lu Guo-Dong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Transarterial chemoembolization (TACE) is the first-line treatment for unresectable intermediate-stage hepatocellular carcinoma (HCC). It is of high clinical significance to explore the synergistic effect of TACE with antiangiogenic inhibitors and the molecular mechanisms involved. This study determined that glucose, but not other analyzed nutrients, offered significant protection against cell death induced by sorafenib, as indicated by glucose deprivation sensitizing cells to sorafenib-induced cell death. Next, this synergistic effect was found to be specific to sorafenib, not to lenvatinib or the chemotherapeutic drugs cisplatin and doxorubicin. Mechanistically, sorafenib-induced mitophagy, as indicated by PINK1 accumulation, increased the phospho-poly-ubiquitination modification, accelerated mitochondrial membrane protein and mitochondrial DNA degradation, and increased the amount of mitochondrion-localized mKeima-Red engulfed by lysosomes. Among several E3 ubiquitin ligases tested, SIAH1 was found to be essential for inducing mitophagy; that is, SIAH1 silencing markedly repressed mitophagy and sensitized cells to sorafenib-induced death. Notably, the combined treatment of glucose restriction and sorafenib abolished ATP generation and mitophagy, which led to a high cell death rate. Oligomycin and antimycin, inhibitors of electron transport chain complexes, mimicked the synergistic effect of sorafenib with glucose restriction to promote cell death mediated via mitophagy inhibition. Finally, inhibition of the glucose transporter by canagliflozin (a clinically available drug used for type-II diabetes) effectively synergized with sorafenib to induce HCC cell death in vitro and to inhibit xenograft tumor growth in vivo. This study demonstrates that simultaneous treatment with sorafenib and glucose restriction is an effective approach to treat HCC, suggesting a promising combination strategy such as transarterial sorafenib-embolization (TASE) for the treatment of unresectable HCC.