De novo transcriptome analysis of Rhododendron molle G. Don flowers by Illumina sequencing

Zheng Xiao,Jiale Su,Xiaobo Sun,Chang Li,Lisi He,Shangping Cheng,Xiaoqing Liu 한국유전학회 2018 Genes & Genomics Vol.40 No.6

Rhododendron molle G. Don occupies an important phylogenetic node in the genus rhododendron with unique yellow flower and medicinal functions. However, only limited genetic resources and their genome information are available for the generation of rhododendron flowers. The next generation sequencing technologies enables generation of genomic resources in a short time and at a minimal cost, and therefore provide a turning point for rhododendron research. Our goal is to use the genetic information to facilitate the relevant research on flowering and flower color formation in R. molle. In total, 66,026 unigenes were identified, among which 31,298 were annotated in the NCBI non-redundant protein database and 22,410 were annotated in the Swiss-Prot database. Of these annotated unigenes, 9490 and 18,680 unigenes were assigned to clusters of orthologous groups and gene ontology categories, respectively. A total of 7177 genes were mapped to 118 pathways using the Kyoto Encyclopedia of Genes and Genomes Pathway database. In addition, 8266 simple sequence repeats (SSRs) were detected, and these SSRs will undoubtedly benefit rhododendron breeding work. Metabolic pathway analysis revealed that 32 unigenes were predicted to be involved in carotenoid biosynthesis. Our transcriptome revealed 32 engines that encode key enzymes in the carotenoid biosynthesis pathway, including PSY, PDS, LCYB, LCYE, etc. The content of β-carotene was much higher than the other carotenoids throughout the flower development. It was consistent with the key genes expression level in the carotenoid biosynthesis pathway by the Illumina expression profile analysis and the qRT-PCR analysis. Our study identified genes associated with carotenoid biosynthesis in R. molle and provides a valuable resource for understanding the flowering and flower color formation mechanisms in R. molle.

Severe Tick Infestation in a Hare and Potential Risk for Transmitting Pathogens to Humans

Weiqing Zheng,Haiying Chen,Xiaoqing Liu,Xuejian Guo,Renlong Fu 대한기생충학열대의학회 2011 The Korean Journal of Parasitology Vol.49 No.4

Experiments and FEM Simulations of Fracture Behaviors for ADC12 Aluminum Alloy Under Impact Load

Yumei Hu,Yue Xiao,Xiaoqing Jin,Haoran Zheng,Yinge Zhou,Jinhua Shao 대한금속·재료학회 2016 METALS AND MATERIALS International Vol.22 No.6

Using the combination of experiment and simulation, the fracture behavior of the brittle metal named ADC12aluminum alloy was studied. Five typical experiments were carried out on this material, with responding datacollected on different stress states and dynamic strain rates. Fractographs revealed that the morphologies offractured specimen under several rates showed different results, indicating that the fracture was predominantly abrittle one in nature. Simulations of the fracture processes of those specimens were conducted by Finite ElementMethod, whilst consistency was observed between simulations and experiments. In simulation, the Johnson-Cook model was chosen to describe the damage development and to predict the failure using parametersdetermined from those experimental data. Subsequently, an ADC12 engine mount bracket crashing simulation wasconducted and the results indicated good agreement with the experiments. The accordance showed that ourresearch can provide an accurate description for the deforming and fracture processes of the studied alloy.

Differences in Clinical Characteristics and Surgical Outcomes of Patients with Ischemic and Hemorrhagic Pituitary Adenomas

Jingpeng Liu,Peng Huang,Xiaoqing Zhang,Yong Chen,Xin Zheng,Rufei Shen,Xuefeng Tang,Hui Yang,Song Li 대한신경외과학회 2023 Journal of Korean neurosurgical society Vol.66 No.1

Objective : Ischemia and hemorrhage of pituitary adenomas (PA) caused important clinical syndrome. However, the differences on clinical characteristics and surgical outcomes between these two kinds apoplexy were less reported. Methods : A retrospective analysis was made of patients with pituitary apoplexy between January 2013 and June 2018. Baseline and clinical characteristics before surgery were reviewed. All patients underwent transsphenoidal surgery and were followed up at least 1 year. Results : Total 67 cases (5.8%) among 1147 pituitary tumor patients were enrolled, which consisted of 28 (~2.4%) ischemic PA and 39 (~3.4%) hemorrhagic PA. There were more male patients in the ischemic group compared with hemorrhagic group (78.6% vs 53.8%, p=0.043). However, the mean age, tumor size and functional tumor ratio were significant higher in the hemorrhagic group. Headache was more common in ischemic PA (82.1%) than that of hemorrhagic PA (51.3%, p=0.011). Magnetic resonance imaging findings found that mucosal thickening and enhancement of the sphenoid sinus was observed in 15 ischemic PA patients (n=27, 55.6%), but none in patients with hemorrhagic PA (n=38, p<0.0001). It was worth noting that the rate of pre-surgical hypopituitarism in ischemic PA patients were seemed higher than that in hemorrhagic PA patients, but not significant. The two groups got a total tumor resection rate at 94.1% and 92.9%, independently. No significant difference on the operative time, blood loss in operation and complications in perioperative period was observed in two groups. After operation, cranial nerve symptoms recovered to normal at 81.8% of ischemic PA patients and 82.6% of hemorrhagic PA patients. Importantly, the incidence of postoperative hypopituitarism partially decreased in both groups, among which the rate of hypothyroidism in ischemic PA patients significantly decreased from 46.4% to 18.5% (p=0.044). Conclusion : Patients with ischemic PA presented different clinical characteristics to the hemorrhagic ones. Transsphenoidal surgery should be considered for the patients with neuro-ophthalmic deficits and might benefit for pituitary function recovery of the apoplectic adenoma patients, especially pituitary thyroid axis in ischemic PA patients.

A highly efficient multi-stage dielectric barrier discharge (DBD)-catalytic system for simultaneous toluene degradation and O3 elimination

Xinyu Hu,Shijie Li,Xiaoqing Dang,Yufei Zhang,Qian Zhang,Huachun Zheng 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.105 No.-

DBD-catalytic system has been widely studied for volatile organic compounds abatement, whereas howto obtain high mineralization rate and low zone emission synchronously remains a challenge. In view ofthis, a new type of multi-stage DBD-catalytic system (MS1) was established to abatement toluene in thisstudy. The mineralization rates of reactors IPC (63.81%) and MS1 (61.14%) were much higher than MS2,PPC1 and PPC2 reactors, which were 48.36%, 46.84%, and 5.8% at 33.8 (Vp-p) kV, respectively. For exportozone concentration, IPC reactor had the highest concentration of 160 ppm, and the values of reactorsMS1, MS2, PPC1, and PPC2 were 79, 34.2, 34.25 and 29 ppm, respectively. The catalyst filled in zone IIcan be utilized to further decompose the residual toluene and intermediates and also promote thedecomposition of ozone, which lead to the superior performance of the MS1 reactor. The influence ofapplied voltage, adsorbed amount, and discharge time on the toluene removal performance was investigatedto optimize the operation parameters of MS1 reactor, their appropriate values were 28.3–31.1 (Vpp)kV, 0.179–0.223 mmol, 1 h, respectively. Lastly, the contribution of disparate zones in multi-stageDBD-catalytic system to the toluene degradation were elucidated on the basis of the GC–MS results.

Death-associated protein kinase 1 phosphorylates MDM2 and inhibits its protein stability and function

Mi Zhang,Xindong Shui,Xiaoqing Zheng,Jong Eun Lee,Yingxue Mei,Ruomeng Li,Yuan Tian,Xiuzhi Zheng,Quling Wang,Long Wang,Dongmei Chen,Tao Zhang,Byeong Mo Kim,Jungho Kim,Tae Ho Lee 대한약학회 2023 Archives of Pharmacal Research Vol.46 No.11

Breast cancer is one of the major malignancies in women, and most related deaths are due to recurrence, drug resistance, and metastasis. The expression of the mouse double minute 2 (MDM2) oncogene is upregulated in breast cancer; however, its regulatory mechanism has yet to be fully elucidated. Herein, we identified the tumor suppressor death-associated protein kinase 1 (DAPK1) as a novel MDM2 regulator by unbiased peptide library screening. DAPK1 is directly bound to MDM2 and phosphorylates it at Thr419. DAPK1-mediated MDM2 phosphorylation promoted its protein degradation via the ubiquitin–proteasome pathway, resulting in upregulated p53 expression. DAPK1 overexpression, but not its kinase activity-deficient form, decreased colony formation and increased doxorubicin-induced cell death; however, DAPK1 knockdown produced the opposite effects in human breast cancer cells. In a xenograft tumorigenesis assay, DAPK1 overexpression significantly reduced tumor formation, whereas inhibition of DAPK1 kinase activity reduced its antitumorigenic effect. Finally, DAPK1 expression was negatively correlated with MDM2 levels in human breast cancer tissues. Thus, these results suggest that DAPK1-mediated MDM2 phosphorylation and its protein degradation may contribute to its antitumorigenic function in breast cancer.

Two-component zeolite-alumina system for toluene trapping with subsequent nonthermal plasma mineralization

Caihong Qin,Mengke Guo,Yang Zheng,Rui Yu,Jiayu Huang,Xiaoqing Dang,Dongjie Yan 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.95 No.-

Two-component zeolite-alumina packed dielectric barrier discharge plasma was developed to deeplymineralize toluene. Compared with packing ZSM-5 and g-Al2O3 separately in layers, uniform mixing ofZSM-5 and g-Al2O3 was more conducive to the mineralization of adsorbed toluene in nonthermal plasma. When the mixing ratio of ZSM-5 to g-Al2O3 was 1:2, the mineralization rate reached 80% afterdischarging for 120 min. Ag supported on the catalyst surface significantly improved the selectivity ofCO2. Increased relative humidity had little effect on the mineralization of adsorbed toluene, butsignificantly reduced the byproducts O3 and N2O. The adsorption and plasma mineralizationperformance of Ag/ZSM-5-g-Al2O3 duringfive cycles of toluene adsorption was stable with only thepeak concentration of CO showing a slight upward trend. GC–MS, TG, and XPS characterization of freshand used catalysts revealed deactivation of Ag/ZSM-5-g-Al2O3 in terms of CO oxidation due tointermediate organic residues and decreased amounts of Ag+ and lattice oxygen on the support ZSM-5instead of g-Al2O3.

Self-bending extrusion molding of distorted channels

Fanlei Min,Huiping Liu,Guangming Zhu,Zheng Chang,Xujie Gao,Bowen Yue,Nana Guo,Xiaoqing Zhai 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.5

Using an integrated profile extrusion and bending forming process with a streamlined extrusion die, a new self-bending extrusion molding technology is proposed with an axis-distorted variable channel. By designing the streamlined extrusion die structure of the distorted central axis, the metal was made to flow non-uniformly in the die cavity, thereby directly extruding a bent profile. The central axis of the streamlined extrusion die is described by a trigonometric function and a Gaussian function. A numerical simulation was applied to analyze the metal flow pattern, equivalent strain, and strain-rate distribution during the self-bending extrusion process. The influences of the extrusion velocity and the addition of a bearing on the self-bending deformation profiles were investigated. During the extrusion process, the streamline at the center of the billet could describe the overall flow of the metal in the die cavity, and the distance between the point on the end face of the die outlet and the center of the die outlet directly determined the degree of extrusion and bending. The greater the distance was, the larger was the degree of bending. The metal strain on the convex edge of the die was greater than that on the concave edge of the die, with the extruded profile always bending toward the concave edge. The strain rate of the metal changed the fastest near the most convex point of the die. As the extrusion velocity increased or more bearings were added, the radius of curvature of the extruded profile increased nonlinearly.

A successive ‘‘conversion-deposition” mechanism achieved by micro-crystalline Cu2O modified current collector for composite lithium anode

Yifei Cai,Bin Qin,Chun Li,Xiaoqing Si,Jian Cao,Xiaohang Zheng,LIANG QIAO,Junlei Qi 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.120 No.-

Lithium (Li) metal is a promising material for high-energy–density batteries, but it is still plagued byobvious capacity degradation and low average Coulombic efficiency resulting from dendrite Li propagation. One main reason is the electro-mechanic coupled failure of plated Li on the current collector, whichcontributes to non-dense Li deposition on the anode. Transition metal oxides (TMOs) with a conversiontypemechanism have been used directly as the anode materials for lithium ion batteries, which demonstratedbetter electro-mechanical stability than metal Li. Herein, a successive ’’conversion-deposition’’mechanism is ingeniously developed to restrain the generation of dendritic Li. Specifically, a microcrystallineCu2O modified current collector was prepared, in which Li+ are sequentially inserted intoCu2O and deposited in the form of Li metal at successive low potential. A Li-Cu half-cell based on thehybrid mechanism sustains a high Coulombic efficiency of over 99.3 % in up to 800 cycles. This work ingeniouslyinhibits the generation of dendrite Li by incorporating conversion-type materials withdeposition-dissolution type metal Li, which contributes to a novel concept for the design of functionalcurrent collectors for composite Li anodes.

A Novel Natural Dye Derivative for Natural Fabric Supercritical Carbon Dioxide Dyeing Technology

Jinsong Wu,Hongjuan Zhao,Mingyue Wang,Weiru Zhi,Xiaoqing Xiong,Laijiu Zheng 한국섬유공학회 2019 Fibers and polymers Vol.20 No.11

The supercritical carbon dioxide (SC-CO2) dyeing technology is a green dyeing technology, and the usage ofnatural dye makes SC-CO2 dyeing technology safer and more environment-friendly. Nevertheless, after using natural dye inSC-CO2 dyeing, the color depth and fastness of dyed natural fabric are poor. In this study, alkyl and hydroxyalkyl groupswere grafted onto alizarin, which is a natural dye, to elevate the color depths and fastness of alizarin-derivative-dyed naturalfabric. The results demonstrate that the color depths of alkyl-alizarin-dyed and hydroxyalkyl-alizarin-dyed natural fabricswere increased. This has to do with the increase in the solubilities of alkyl alizarin and hydroxyalkyl alizarin in SC-CO2. Thehydroxybutyl-alizarin-dyed wool displayed the best color depth (K/S value: 6.44). And the hydroxyalkyl-alizarin-dyednatural fabric showed good washing fastness and rubbing fastness (about 4-5 level), because that hydroxyalkyl alizarin couldbe linked by a covalent bond to the natural fabric.