Spatiotemporal expression patterns of thymosin and its immune regulation after bacterial stimulation in American cockroach (Periplaneta americana)

Yang Zhen,Xie Jiqin,Yang Yu,Sun Xiaohong,Jing Jie,Shen Yongmei,Yue Bisong,Zhang Xiuyue 한국곤충학회 2021 Entomological Research Vol.51 No.12

Thymosin is a physiologically active polypeptide hormone which has many functions involved in promoting wound healing, promoting hair growth, and accelerating tissue and organ regeneration. American cockroach (Periplaneta americana) has three subtypes thymosin (THY1, THY2, THY3) which are different splices from the same thymosin gene. Real-time PCR was used to detect and analyze the expression differences of THY1 and THY2 in different stages, sexes, tissues and after stimulation by bacteria. Thymosins were expressed in adult, eclosion, and ootheca, and their expression levels were significantly up-regulated during the eclosion period, showing the thymosin might be involved in the eclosion process. The expression levels of thymosins in females were higher than males, and the differences in eclosion stages were more significant (P < 0.01), indicating that they were closely related to development and female reproduction. The expressions of THY1 and THY2 in hemolymph were significantly higher (P < 0.01) than that in other tissues. The up-regulated expression of thymosin in two immune-related tissues (Hemolymph and adipose) after Escherichia coli stimulation further confirmed its involvement in the immune response. Our research would provide a basis for screening of novel pesticides target genes.

A Novel Approach Based on Fault Tolerance and Recursive Segmentation to Query by Humming

Xiaohong Yang,Qingcai Chen,Xiaolong Wang 보안공학연구지원센터 2010 International Journal of Hybrid Information Techno Vol.3 No.3

Glutamic-oxaloacetic transaminase 1 regulates adipocyte differentiation by altering nicotinamide adenine dinucleotide phosphate content

Yang Yang,Cheng Zhimin,Zhang Wanfeng,Hei Wei,Lu Chang,Cai Chunbo,Zhao Yan,Gao Pengfei,Guo Xiaohong,Cao Guoqing,Li Bugao 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.2

Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation. Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content. Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p< 0.01), whereas GOT1 overexpression significantly increased lipid accumulation (p<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content. Conclusion: The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism. Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation.Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content.Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p<0.01), whereas GOT1 overexpression significantly increased lipid accumulation (p<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content.Conclusion: The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism.

Involvement of Orai1 in tunicamycin-induced endothelial dysfunction

Yang, Hui,Xue, Yumei,Kuang, Sujuan,Zhang, Mengzhen,Chen, Jinghui,Liu, Lin,Shan, Zhixin,Lin, Qiuxiong,Li, Xiaohong,Yang, Min,Zhou, Hui,Rao, Fang,Deng, Chunyu The Korean Society of Pharmacology 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.2

Endoplasmic reticulum (ER) stress is mediated by disturbance of $Ca^{2+}$ homeostasis. The store-operated calcium (SOC) channel is the primary $Ca^{2+}$ channel in non-excitable cells, but its participation in agent-induced ER stress is not clear. In this study, the effects of tunicamycin on $Ca^{2+}$ influx in human umbilical vein endothelial cells (HUVECs) were observed with the fluorescent probe Fluo-4 AM. The effect of tunicamycin on the expression of the unfolded protein response (UPR)-related proteins BiP and CHOP was assayed by western blotting with or without inhibition of Orai1. Tunicamycin induced endothelial dysfunction by activating ER stress. Orai1 expression and the influx of extracellular $Ca^{2+}$ in HUVECs were both upregulated during ER stress. The SOC channel inhibitor SKF96365 reversed tunicamycin-induced endothelial cell dysfunction by inhibiting ER stress. Regulation of tunicamycin-induced ER stress by Orai1 indicates that modification of Orai1 activity may have therapeutic value for conditions with ER stress-induced endothelial dysfunction.

Involvement of Orai1 in tunicamycin-induced endothelial dysfunction

Hui Yang,Yumei Xue,Sujuan Kuang,Mengzhen Zhang,Jinghui Chen,Lin Liu,Zhixin Shan,Qiuxiong Lin,Xiaohong Li,Min Yang,Hui Zhou,Fang Rao,Chunyu Deng 대한약리학회 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.2

Endoplasmic reticulum (ER) stress is mediated by disturbance of Ca2+ homeostasis. The store-operated calcium (SOC) channel is the primary Ca2+ channel in non-excitable cells, but its participation in agent-induced ER stress is not clear. In this study, the effects of tunicamycin on Ca2+ influx in human umbilical vein endothelial cells (HUVECs) were observed with the fluorescent probe Fluo-4 AM. The effect of tunicamycin on the expression of the unfolded protein response (UPR)-related proteins BiP and CHOP was assayed by western blotting with or without inhibition of Orai1. Tunicamycin induced endothelial dysfunction by activating ER stress. Orai1 expression and the influx of extracellular Ca2+ in HUVECs were both upregulated during ER stress. The SOC channel inhibitor SKF96365 reversed tunicamycin-induced endothelial cell dysfunction by inhibiting ER stress. Regulation of tunicamycin-induced ER stress by Orai1 indicates that modification of Orai1 activity may have therapeutic value for conditions with ER stress-induced endothelial dysfunction.

The Design of Anti-aliasing Analog Filter for Data Acquisition in the Surface Measurement

Xu Jingbo,Xu Xiaohong,Yang Pengyu,Lin Haijun,Guo Xin 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5

In the surface measurement system, the data acquisition is key part and the anti-aliasing analog filter is necessary. This paper deals with the design of the anti-aliasing analog filter. Based on the principle of anti-aliasing filtering, the parameters of filter are computed, the filter circuit is designed, and the frequency characteristic is drawn. Combined with digital filter, the filtering can maintain the low frequency components very well and suppress effectively the high frequency signals in the original surface profile, which reduces the distortion caused by noise and makes the filtering effect better. This method has been applied in the surface measurement system and the actually measured data verified the performance of the filter.

Prediction model of the surface roughness of micro-milling single crystal copper

Xiaohong Lu,Liang Xue,Feixiang Ruan,Kun Yang,Steven Y. Liang 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.11

Presently, the demand for single crystal copper micro-components is increasing in various fields because single crystal copper has good electrical conductivity. Micro-milling technology is an effective processing technology for small single crystal copper parts. Surface roughness is a key performance indicator for micro-milling single crystal copper. Establishing a surface roughness prediction model with high precision is useful to guarantee the processing quality by selecting the cutting parameters for micro-milling. Few studies have currently focused on micro-milling single crystal copper. In this study, the orthogonal experiments of micro-milling single crystal copper were conducted, and the influences of the spindle and feed speeds and axial depth of cut on the surface roughness of micro-milled single crystal copper with different orientations were analyzed by range analyses. The spindle rotation speed was the major affecting factor. The surface roughness of single crystal copper in different crystal orientations was predicted by using the SVM method. Experimental results showed that the average relative error of the surface roughness of <100>, <110>, and <111> crystal orientation single crystal copper was 2.7 %, 3.3 %, and 2.2 %, respectively, and that the maximum relative errors were 7.0 %. 10.1 %, and 3.1 %, respectively. The uncertainty analysis was conducted by using the Monte Carlo method to verify the reliability of the built surface roughness model.

Effects of Annealing Pressures on the Ordering and Microstructures of FePt

Xiaohong Li,Zhaodi Feng,Yang Li,Wenpeng Song,Qian Zhang,Baoting Liu 한국자기학회 2013 Journal of Magnetics Vol.18 No.4

FePt:Ag (100 nm) nanocomposite thin films were prepared on naturally-oxidized Si substrates by dc magnetron sputtering at room temperature. X-ray diffraction (XRD) and transmission electron microscopy (TEM) are used to investigate the effects of annealing pressures on the ordering processes and microstructures of these films. The average sizes for the L10 ordered domains and the FePt grains are reduced to d = 9 nm and D = 13 nm from d = 19 nm and D = 34 nm, respectively, when the annealing pressure is enhanced to 0.6 GPa from room pressure at 873 K. Furthermore, the size distribution is improved into a narrow range. With increasing pressure, the coercivity of L10-FePt:Ag thin films decreases from 15.1 to 7.6 kOe. In the present study, the effects of high pressure on the L10 ordering processes and microstructures of FePt:Ag nanocomposite films were discussed.

Simultaneous regulation of photoabsorption and ferromagnetism of NaTaO3 by Fe doping

Huan Yang,Liguo Zhang,Lifang Yu,Fang Wang,Zhenzhen Ma,Jie Zhou,Xiaohong Xu 한국물리학회 2018 Current Applied Physics Vol.18 No.11

NaTa1-xFexO3 (0≤x≤0.40) nanocubes were synthesized by a relatively low temperature hydrothermal method, using Ta2O5, FeCl3 and NaOH as the precursors. The UV–vis diffuse reflectance spectra showed that NaTa1-xFexO3 had significant visible-light-absorbing capability, and the absorption edge of NaTaO3 shifted to longer wavelength with the increase of Fe dopants. Moreover, NaTa1-xFexO3 exhibited room-temperature ferromagnetism when Fe3+ occupied Ta5+ sites in NaTaO3 crystal lattice. The ferromagnetism is mainly attributed to the superexchange interactions between doped Fe3+, rather than the contribution of oxygen vacancies caused by Fe doping. Therefore, Fe doping can simultaneously regulate the optical and magnetic properties of NaTaO3 semiconductor, which will enable its potential applications in multifunctional optical-electronics and opticalspintronics devices.

Ozone infusiblization and curing mechanism of polysilazane ceramic precursor fibers

Li Xiaohong,Luo Xiaoyu,Li Jing,Li Jinxia,Yang Jiahao,Ahmad Zahoor,Bao Zhihao,Zhang Xiao,Chen Jianjun 한국세라믹학회 2023 한국세라믹학회지 Vol.60 No.5

An appropriate infusible method plays a vital role as one of the key processes of the fabrication of polymer-derived ceramic fibers. In this work, ozone curing, a novel room temperature controllable curing strategy was used to prepare SiCN ceramic fibers. Some circular pores were observed on the surface of the obtained SiCN ceramic fibers. The tensile strength of the fibers was also investigated. To reveal the ozone-curing mechanisms of the PSZ fibers and the formation process of the circular pores, the FT-IR, SEM, OM, XRD and TG were characterized. The results showed that the Si–H bonds and Si-CH3 groups in the PSZ molecule were oxidized gradually to form oxygen-containing groups such as Si–OH, Si–O-Si and C = O in the ozone curing process, which contributed to the curing and weight gain of the PSZ fibers. Moreover, a hydrolysis reaction between Si-NH-Si and H2O might further accelerate the curing of the PSZ fibers. An unexpected result of the hydrolysis reaction is the formation of some circular pores on the surface layer of the PSZ fibers. This strategy provides a method to design, adjust and control the microstructure and composition of silicon-based ceramic fibers, and the obtained SiCN fibers may be used as catalyst support, bacterial culture and other fields.