응급진료시 여성환자의 소변검체 채취방법

박원녕,정상원,이종호,구홍두,심호식,김승호 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Urinalysis is a useful laboratory test in the diagnosis of various diseases. In sampling for the urinalysis, there is much concern about contamination that can lead to misdiagnosis in the mid-stream urine sampling method. We conducted this study to determine, in terms of concordance of results and contamination in culture, whether there was any superiority in nod-stream sampling methods with or without disinfection measures compared to the catheterization method. Materials and Methods: We used three kinds of urine sampling methods sequentially, mid-stream non-clean catch, mid-stream clean catch, and catheterization, for ambulatory, non-pregnant, non-men-struating female patients who visited NHIC Ilsan Hospital emergency department during a one-week period in September 2001. Each sample was electrophotometrically analyzed for leukocyte esterase, nitrite, and blood by using a reagent strip and was cultured immediately or after overnight refrigeration. Results: Of the 41 patients, 9 had culture-proven urinary tract infections. The concordance rates(kappa) for nitrite, blood, and leukocyte esterase were 0.875, 0.403, and 0.406 between non-clean catch and catheterized samples and 0.875, 0.481, and 0.560 between clean catch and catheterized samples, respectively. The contamination rate of the non-clean catch, the clean catch, and the catheterized samples were statistically different: 51.2%, 29.3% and 0%, respectively. Conclusion: We found that cleaning with disinfectant was effective for reducing the contamination rate, even though the best urine sampling method for zero contamination was catheterization. We recommend considering cost, patients' comfort, an acceptable threshold for contamination, and the necessity for a culture before choosing a urine sampling method for women who visit the emergency department.

Omeprazole 투여시 위산분비 억제능에 대한 CYP2C19 의 영향

박수헌(Soo Heon Park),김종원(Jong Won Kim),이수연(Soo Youn Lee),최명규(Myung Gyu Choi),김재광(Jae Kwang Kim),정인식(In Sik Chung),정규원(Gyu Won Chung),선희식(Hee Sik Sun),박두호(Doo Ho Park) 대한소화기학회 2001 대한소화기학회지 Vol.38 No.6

Background/Aims: CYP2C19 has an important role in the catabolism of several proton pump inhibitors. However, the relative contribution of CYP2C19-mediated metabolism varies among the different proton pump inhibitors. We investigate to determine the effect of CYP2C19 genotype status on intragastric pH during dosing with omeprazole. Methods: The subjects were 16 healthy volunteers. Their CYP2C19 genotype status was determined by a polymerase chain reaction-restriction fragment length polymorphism method. Twenty-four-hour monitoring of intragastric acidity were performed on the day without medication and day 4 after omeprazole administration. Results: A single dose of omeprazole significantly decreased 24 hour intragastric acidity (1.7±0.3 vs. 5.1±0.4). Subjects were divided into three groups on the basis of their CYP2C19 genotype status: homozygous extensive metabolizers (Ho-EMs, n=5), heterozygous extensive metabolizers (Ht-EMs, n=6), and poor metabolizers (PMs, n=5). Median 24 hour intragastric pH in the Ho-EM group was 3.1 compared with 5.5 in Ht-EM group and 5.9 in PM group(P<0.05). The median pH during omeprazole administration was influenced by CYP2C19 genotype. On the other hand, the Helicobacter pylori infection did not influence the median intragastric pH during omeprazole administration (p= NS). Conclusions: The effects of omeprazole on intragastric acidity is influenced by the CYP2C19 polymorphism. (Korean J Gastroenterol 2001;38:399-404)

신규 방사성 항암제 DW-166HC 의 소핵시험

문은이(Eun Yi Moon),이진(Jin Lee),이원용(Won Yong Lee),최청하(Chung Ha Choi),이덕근(Dug Keun Lee),유제만(Jei Man Ryu),정용호(Yong Ho Chung),윤성준(Sung June Yoon),박경배(Kyung Bae Park) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.3

DW-166HC (^(166)Holmium (^(166)Ho)-Chitosan complex) is a new radiopharmaceutic anticancer agent with a broad anti-tumorigenic spectrum, especially against human hepatic cancer. DW-166HC was evaluated for the appearance of micronucleus in polychromatic erythrocytes (PCEs) of mouse bone marrow cells after subcutaneous arid intravenous single administration. Bone marrow cells were prepared at 24 hr and 48 hr after DW-166HC-I (^(165)Ho-Chitosan complex : cold compound) administration and at 24 hr, 72 hr and 2 weeks after DW-166HC (^(166)Ho-Chitosan complex : hot compound) administration. The results showed there was no statistically significant increase of the numbers of PCEs with micronucleus in all DW-166HC-I administered groups compared with a negative control group but there was statistically significant increase of the numbers of PCEs with micronucleus at 24 hr arid 72 hr in all DW-166HC administered groups, which was recovered after 2 weeks from the drug administration. The results also showed the ratio of normochromatic erythrocytes (NCEs) to PCEs of all DW-166HC-I administered groups was not significantly different from that of a negative controi group but there was significant difference of this ratio at 24hr and 72 hr in all DW-166HC administered groups compared with that of negative group, which was also recovered after two weeks from the drug administration. These results suggested that DW-166HC-I may not cause any chromosomal damage but DW-166HC has in vivo mutagenic potential because of its radioactivity.

고등학교 지구과학 실험·실습 요목 비교 분석

정원우,이윤종,기우항,김영호,양승영,강용희,안병호,임성규,윤일희,김중욱,윤성효,강동진 경북대학교 과학교육연구소 1997 科學敎育硏究誌 Vol.21 No.-

The purpose of this study is to investigate the status of the experimental and practical education in high school earth. The present status and reasonable management of the experimental and practical education in high school earth science have been grasped from the questionaires. To do this, eighty eight earth science teachers in Korea are administered questionaires. The frequency of the experimental and practical items in the seven kind of earth science text books were investigated. The problems and the reasonable management for experimental and practical education were proposed in this paper.

최근 발병한 정신분열병 환자의 증상 관해 및 정신사회적 기능 : 1년 전향적 추적 관찰 연구

정석훈,박원명,권준수,박지홍,안준호,고진경,김창윤 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

Objectives : The long-term outcome of schizophrenia is still considered vahable and inconclusive. We perfonned a one-year prospective observational study to investigate the longitudinal outcomes in patients with recent-onset schizophrenia. The primary purpose was to determine the descriptive outcomes in tenns of symptom remission and psychosocial function. The secondary purpose was to identify predictor variables associated with the outcomes. Methods : Patients experiencing their first episode ofpsychosis or hospitalization within the past 2 years with a diagnosis of DSM-IV schizophrenia were included. Clinical symptoms were assessed monthly using Brief Psychiatric Rating Scale (BPRS), Scale for the Assessment of Positive Symptoms (SAPS), Scale for the Assessment of Negative Symptoms (SANS) and Clinical Global Impression (CGI) scale. Psychosocial function was measured using PSYCH-UP (Psychosocial symptoms you currently have, follow-up version) and Heinrichs' Quality of Life Scale (QLS) at baseline, 6, and 12 months and Global Assessment of Functioning (GAF) monthly. Remission was defined on the basis of overall, and psychotic core symptoms as having 1) BPRS total score ≤ 36, and 2) Each individual item score of core psychotic items (conceptual disorganization, suspiciousness, hal-lucinatory behavior, unusual thought content) ≤3, and 3) CGI severity score ≤3, and 4) Duration of remission at least for 2 consecutive months. To identify the predictive variables for the outcome, relationships between time to remission and various clinical variables were examined using Cox proportional hazards model. Results : A total of 49 patients with schizophrenia were enrolled in this study. The proportions of patients with symptom re-mission were 54.5% (24/44, drop outs=5) at 3 months, 55.3% (21/38, drop outs=11) at 6 months, and 64.3% (18/28, drop outs=21) at 12 months. Mean time to remission was 3.76±2.43 (s.d.) months, and the mean duration of remission was 7.54±2.99 months. Significantly longer time to remission was predicted by higher SANS total score at baseline in various predictor variables (p=0.01). Impairments of psychosocial function measured using PSYCH-UP and QOL were significantly improved at the end of this study, but still remained at mild to moderate level. SANS total score and GAF score significantly correlated with most ofthe items related to psychosocial fimction at 12 months. The proportion of the patients with GAF score ≥60 increased from 6.1% at baseline to 85.8% at 12 months. Of the patients who were in remission at 12 months, 95.5% obtained GAF score ≥60. Conclusion : This study showed 64.3% of symptom remission rate in patients with recent-onset schizophrenia. Symptom re-mission was accompanied by significant improvement ofglobal function. The severity of negative symptom at baseline appeared to be a significant predictor for time to remission. Psychosocial function was improved at the end of this study, but impainnents still remained at mild to moderate level. GAF score and negative symptoms significantly correlated with psychosocial function.

만성신부전환자의 구강위생상태에 관한 역학적 조사연구

정호균,박주현,홍지원,강홍구 중앙대부설한국의과학연구소 1991 한국의과학 Vol.23 No.1

전정기능검사에서 정상소견을 보이는 어지러운 환자들의 임상적 특징

정원호,방은국,홍성화,이치규,이현석 대한평형의학회 2002 Research in Vestibular Science Vol.1 No.2

精神症狀에 대한 韓國人의 態度調査

元鎬澤,洪元植,張煥日,陳聖太,金光日,李基南,嚴龍燮,金明正 大韓神經精神醫學會 1973 신경정신의학 Vol.12 No.1

Korean psychiatrists are more apt to take the abnormal drinking behavior lightly and regard the psychosomatic symptoms as more serious, comparing with American psychiatrists. On the other hand, physicians as well as adults from the population in Korea are less concerned about the neurotic and psychosomatic symptoms and alcoholic behavior as psychiatric problems. It is the authors' concern to understand such differences in opinion about abnormal behaviors from the cultural and social psychiatric view point. The authors used Dohrenwend's questionaire form of 45 mental symptoms and three additional items i. e., homosexual behavior, symptom of senile psychosis and delusion of jealousy following the authors' interests. Each item was rated by three point cale (psychiatrically very serious=2 points, somewhat serious=1, not serious at all=0) and evaluated with critical ratio. As a preliminary study, the reliability of this questionaire was confirmed by the test-retest method; Spearman's rho was 0,93. The subjects in this study consist of 50 psychiatrists, 49 physicians, 54 herb practitioners and 154 adults from the normal population(60 urban samples and 94 rural samples). The collection was carried out by individual interviews for the physicians and normal samples, by letters for the psychiatrists and the herb practitioners. And the data of American psychiatrists compared in this study were quoted from Dohrenwend's report(27). This study has two parts: one is the cross-cultural comparison between American psychiatrists and Korean ones, the other is intracultural comparison among psychiatrists, physicians, herb practitioners and normal population samples. Inspite of the general notion that Korean psychiatry has been much oriented to American psychiatry for over twenty years, there are definite differences in opinion about the severity of abnormal behaviors between American and Korean psychiatrist, especially about alcoholic behavior and psychosomatic symptoms. Such differences are possibly interpreted as arising from the cultural differences. The American psychiatrists seem to be more concerned about alcoholic behavior probably due to the strict attitude of people and/or the fact that the United States reveals the highest incidence of alcoholism throughout the world. The Korea psychiatrists, however, have much tolerable attitude toward alcoholic behavior according to the cultural tradition. Solitary drinking is hardly found in Korea; drinking in Korea is a suitable way of interpersonal contact. They drink slowly and are not so intensively dependent upon alcohol itself. Dependency need to alcohol can be diffused to the other persons. And they take alcohol with many kinds of side-dishes. These drinking manners seem to be cultural virtues for low incidence of alcoholism in Korea. Thus, Korean psychiatrists as well as adults from normal population have highly tolerable attitude toward alcoholic behavior. Even when some person reveals a destructive behavior after drinking, they grant it as a personal charm. Korea psychiatrists, therefore, are not so nervous about the alcoholic behavior. On the other hard, the psychosomatic problems seem to be serious in Korean mental patients. They are easily apt to express their emotional problems as somatic symptoms possibly due to the disease concept of oriental herb medicine that has a high tendency of projection of emotional conflicts unto the soma. So most of Korean people are not aware that psychosomatic symptoms are originated from the emotional problems, and quite contrary to it, the psychiatrists may be highly conscious of psychosomatic symptoms. Another significant difference is seen in suicidal idea and suspiciousness: American psychiatrists regard them as more serious, and reversely Korean psychiatrists regard them as less serious. Such a pattern may also be derived from the socio-cultural differences. When Koreans are either dissapointed or delighted, they say frequently "I want to die" or "It's funny to death" and so on. This idiomatic expression is far from the real death wish but rather an expression of magnification. And the suicidal idea itself is frequently seen in normal population even suicidal attempt is evaluated as abnormal in Korea. With such reasons, Korean psychiatrists may notice suicidal idea as less serious than American psychiatrists. And the suspiciousness may be a way of living for the people under the historical turmoil and vigorous aculturation, that it is perceived as not so abnormal. The next problem is the marked discrepancy of opinion about abnormal behaviors among psychiatrists, physicians, herb practitioners and normal population samples. The agreement of physicians, herb practitioners, urban samples and rural samples with the psychiatrists on the relative severity of symptoms revealed as Spearman's rho of 0.79, 0.59, 0.58 and 0.44 respectively. It is the main point of problem that physicians are less aware of neurotic symptoms which can appear in the somatic illness, and of psychosomatic symptoms as psychiatric problem. Possible reason is that Korean physician have been oriented to the somatic medicine without proper psychosomatic orientation. On the other hand, the herb practitioners are apt to ignore the severity of psychotic symptoms and on the other, regard the neurotic and psychosomatic symptoms as much as psychiatrists' opinion. And the samples of normal population regard the neurotic symptoms as more serious than the psychotic symptoms, and are not aware of the psychosomatic symptoms as psychiatric problems. Physicians, herb practitioners and samples from the normal population underestimate the alcoholic behavior more than psychiatrists do. The alcoholism according to physicians' opinion is rather somatic problem than psychological one. The herb practitioners and adults from normal population manifest the prominently tolerable attitude toward alcoholic behavior. In conclusion, two kinds of problems can be suggested. One is that the difference between Korean and American psychiatrists in the evaluation on the severity of abnormal behaviors, is possibly due to sociocultural differences. The other is the intracultural difference among psychiatrists, physicians, herb practitioners and adults from normal population. Such a difference suggests that there is a considerable cultural lag in the disease concept and attitude between the modern psychiatry and the cultural tradition. This discrepancy can result in the undesirable wandering of patients for seeking the treatment and some hardship in modern psychiatric practitioning in Korea.

醱酵食品添加物의 能力 및 安全性確認 硏究 : -第二報 乳酸菌製劑食品의 活性能力試驗- -PartⅡ Activity Test of Lactic Acid Bacterial Food and Drugs-

鄭鎬權,姜孝源,權泰鍾 건국대학교 1978 學術誌 Vol.22 No.1

This work was carried out to investigate the acid producing activity, tolerance and other important characteristics of the strains isolated from the market yogurt drinks (fermented milk or fomented milk drinks) and lactic bacterial drugs (control agent of entero-bacteria). And obtained results were as in the followings. 1. Almost of the strains isolated from market yogurt drinks were identified as Lactobacillus bulgaricus and two of them were Lactobacillus acidophillus. The specially aerobic strains isolated from lactic acid bacterial drugs were identified as Bacillus subtilis and anaerobics were Clostridium butylicum, Sporolactobacillus inulinus, Streptococcus faecalis and Bifidobacterium bifidum. 2. Concentration of active lactic acid bacteria of the market yogurt drinks was found almost as same level, and decreasing tendency of the concentration was commonly seen in every samples during hot summer (high temperature over 35℃). Acid producing activity of some stronger strains continued for 10 days and weak strains for 6 days in summer. 3. During 6 months of the test period, not any contamination case of pathogenic enter-obacteria was found in market yogurt drink samples. 4. But yeast contamination was so aboundantly seen that the ratio of contaminated case was attained to about 20% in fermented milk and to 80% in fermented drink samples. 5. Maximum acid production of the strains isolated from market yogurt drinks was attained to almost 2.0%, but that of the strains from tactic acid bacterial drugs was less than 0.7% except the Str. faecalis strain. 6. Curd forming and acid producing activities of both L. bulgaricus strains and L. acidophillus strains were almost as same level. And the activities continued for 15 days at temperature of 5℃ and pH of 4.0, but for 7 days at high temperature of 35℃. 7. In high temperature (over 35℃) and loll pH condition (less than 3.6), the old culture of homo fermentative lactic acid bacterial strains isolated from market yogurt drinks were more easily changed to hetero fermentative strains than the young culture of the strains.

Interleukin-2가 Mouse간의 인산염분해효소의 활성에 미치는 영향

이규식,김원규,정호삼,강성섭 한양대학교 의과대학 1989 한양의대 학술지 Vol.9 No.1

In recent years, interleukin-2, a lymphokine produced by antigen or mitogenstimulated lymphocytes, has been intensively with the development of immunooncology and chemoimmunotherapy of the malignant tumor. Interleukin-2 mediates antitumor effect by stimulating the proliferation of helperand cytotoxic T cells, augmenting the cytolytic activities of cytotoxic T-lymphocytes and differentiating T-lymphocytes into a lymphokine-activated killer cell in vivo. Thus interleukin-2 induces the therapeutic effects on various sarcoma and carcinoma and regresses the pulmonary, hepatic and peritoneal metastases of various tumors. Although interleukin-2 is very effective for the treatment of various malignancy, it develops many side effects including fever, chill, malaise, mental confusion, diarrhea, vomiting and oliguria and causes pulmonary, hepatic and renal toxicities. Especially interleukin-2 results in increase of capillary permeability with weight gain, pulmonary edema, and ascites. In this experiment, the author studied the effect of interleukin-2 on the mouse liver, histochemically observing the changes in the activities of alkaline phosphatase, acid phosphatase and adenosine triphosphatase. Male mice, DDY strain, weighing 20gm, were used as experimental animals. The experimental animals were sacrificed at 3, 6, 12, 24 and 48 hours after intraperitoneal administration of two milions unit of interleukin-2 per kilogram of body weight. The specimens obtained from the liver were fixed in 10% neutral buffered formalin solution for 24 hours at 4℃ and sliced at 16㎛ thickness in a frozen cryostat. The activities of alkaline phosphatase and acid phosphatase were observed by the Gomori's method and the activity of adenosine triphosphatase was observed by the Wachstein and Meisel's method for histochemical study. The results obtained were as follows. 1. The acid phosphatase activity was strong positive at 12 hours and 24 hours groups in the central and portal zones of the liver lobules, but weak positive and moderate positive at 48 hours group in the central and portal zones of the liver lobules, respectively. 2. The alkaline phosphatase activity was weak positive at 6 hours and 12 hours groups and moderate positive at 48 hours group in the central and portal zone of the liver lobules. 3. The adenosine triphosphatase activity was trace positive in the central zone and weak positive in the portal zone of the liver lobules at 6 hours group but moderate positive at 48 hours group in the central and portal zones of the liver lobules. Consequently, it is suggested that interleukin-2 induce degenerative changes of the hepatocytes of the mouse, but it is recovered with time.