Comparison of stomach microRNA transcriptomes of Tibetan and Yorkshire pigs by deep sequencing

Wen‑kui Sun,Yanyue Li,Chi Cheng,Yi‑hui Chen,Kai Zeng,Xiaohui Chen,Yiren Gu,Rui Liu,Xuebin Lv,Rong Gao 한국유전학회 2018 Genes & Genomics Vol.40 No.9

MiRNAs regulate the expression of target genes in diverse cellular processes and hence play important roles in different physiological processes, yet little is known about the stomach microRNAome (miRNAome) of the Tibetan pig. The objective of this experiment was to investigate differentially expressed stomach miRNAs participating in digestion. Firstly, we isolated total RNA by Trizol reagent from three Tibetan and three Yorkshire purebred pigs stomach samples at 90-day-old. Secondly, a comprehensive analysis of Tibetan and Yorkshire pig stomach miRNAomes was performed by small RNA sequencing in the Illumina HiSeq 2000 system. Finally, SYBR Green Real-time RT-PCR was performed to validate the differentially expressed miRNAs. We identified 318 unique miRNAs, 260 were co-expressed in both libraries, 17 and 31 miRNAs were specifically expressed in Tibetan and Yorkshire pigs respectively. Fifty six differentially expressed miRNAs were identified by the identifying differentially expressed genes 6 (IDEG6). Kyoto encyclopedia of genes and genomes analysis revealed that some of the differentially expressed miRNAs were associated with protein and fat digestion. Two differentially expressed miRNAs (miR-214-3p and ssc-un39) participating in the digestion of lipid were identified. Additionally, qRT-PCR results suggested that a higher expression of miR-214-3p in the Tibetan pig stomach could lead to relatively lower expression of calcium-dependent phospholipase A2, which is an enzyme important for the digestion of glycerol phospholipid. This study has delineated the different stomach miRNAs expression patterns of Tibetan and Yorkshire pigs, which would help explain the regulatory mechanisms of miRNAs in digestion of Tibetan pigs, and contribute to utilize a the unique digestion merits of Tibetan pig in future porcine hybridization breeding.

Investigation of Cu Interlayer on Joint Formation of Ti/Mg Bimetal Fabricated by Liquid–Solid Compound Casting Process

Fulin Wen,Jianhua Zhao,Kaiqing Feng,Miaowang Yuan,Dengzhi Zheng,Cheng Gu,Bei Xu 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.7

In this paper, TC4/AZ91D bimetallic composites has been prepared by liquid–solid compound casting process adaptingvarious thickness of Cu coating. The effects of Cu coating thickness on the evolution of interface microstructures and jointmechanical properties are investigated. The results indicate that metallurgical bonded joints are obtained with Cu coatingthickness ranging from 36.7 to 51.4 μm. With the increase of Cu coating thickness, the interface microstructure evolvesfrom δ-Mg + Mg2Cueutectic structure to Mg–Cu intermetallic compound (IMC) Cu2Mg+ Mg2Cuand Mg–Al–Cu ternaryintermetallic compound. The calculation results of formation enthalpy and chemical potential of Mg–Al–Cu system suggestthat Cu element prefers to react with Mg element and formed Mg–Cu IMC. In particular, when Cu coating thickness reaches36.7 μm, the average shear strength of the bimetal reaches a maximum of 65.3 MPa. Further increasing Cu coating thicknessleads to the generation of thick Mg2CuIMC layer and Mg2Cu+ Cu2Mgmixed IMC layer which are proved to be harmful tothe shear strength of TC4/AZ91D bimetals. All the fracture surfaces of the bimetallic composites exhibite to have a brittlefracture morphology. However, the fracture location is different with each other. For Cu coating thickness of 36.7 μm, theinterface fractures at the δ-Mg + Mg2Cueutectic structure, while the interface fractures at the Cu2Mg+ Mg–Al–Cu ternaryintermetallic layers when Cu coating thickness is 44.2 μm and 51.4 μm.

《紅樓夢》特色詞語近親探源

董文成 중국어문연구회 2001 中國語文論叢 Vol.20 No.-

≪홍루몽≫ 은 작자 조설근의 독창적 이미지의 창출로 인하여 고금의 독자들로부터 크게 환영받고 있는데, 그러한 특징적인 이미지가 사실은 중국 고전의 발전적 계승이라는 면에서 작가의 폭넓은 고전 지식과 함께 소설 창작의 뛰어난 기교를 드러낸다고 할 수 있다. 본고에서는 특히 명말청초에 일세를 풍미했 던 재자가인소설과 염정소설 속에서 이미 부분적으로 활용되었던 이미지들이 ≪홍루몽≫속에서 어떻게 수준높은 이미지로 재창출되었는가를 다음과 같이 구체적인 예증을 들어 밝혀주고 있다 1. 작품 속의 두 여주인공 설보차와 임대옥의 이미지로 자리매김한 '山中高士晶□雪'과 '世外仙□寂□林'의 시구는 명초 高啓의 시에 그 근원을 두고 있지만 사실은 청초의 염정소설 ≪濃情快史≫ 에서 이미 구체적으로 사용된 바 있다. 2. 주인공 가보옥과 임대옥의 애틋한 감정이 구체적으로 표현되고 있는 제19회의 회목 '情切切良育花解語, 意綿綿靜日玉生香'의 이미지는 ≪金□梅詞話≫에서 사용한 시구에서 이미 사용되고 있다. 3 임대옥의 이미지를 가장 잘 드러내는 특징 언어의 하나로 花魂이 있는데 작가가 그녀를 묘사할 때나 그녀 스스로 지은 <葬花詞>에서 이 말을 사용하고 있다. 하지만 청초에 유행한≪금운□전≫≪春燈關≫, ≪巫山□史≫등의 작품 속에서 이미 만들어져 있었음을 확인하였다. 4. ≪홍루몽≫ 첫 회의 유래고사에는 女□신화를 이용하면서 補天遣石 하나가 大流山 靑漢峰에 버려지게 되었다고 하였는데 脂硯齋 평어에서 이미 靑□은 이와 발음이 동일한 情根의 의미를 가졌다고 지적한 바 있다. 오늘날 이 구절은 ≪홍루몽≫의 독특한 이미지의 구축에 결정적인 역할을 하고 있지만 사실 청초 順治, 康熙연간에 나온 ≪桃花影≫, ≪□花叢≫, ≪巫山□史≫, ≪春燈□≫등에서 자주 사용되어 왔음을 확인할 수 있다. 5. 유래고사에 나오는 대황산에 버려진 □石은 주인공 가보옥의 삶의 원형으로서 一僧一道에 의해 통령보옥으로 환생되어 ≪홍루몽≫ 만이 가진 독특한 예술 이미지를 창출한 특별한 어휘라고 할 수 있는데 사실 청초 염정소설 ≪杏花天≫ 과 ≪春燈□≫ 등에서 이를 사용한 바 있다. 6. ≪홍루몽≫의 또 다른 이름으로 작자 스스로 제시한 이름 중에 "金陵十二□"가 있는데 가보옥의 '夢遊太虛濾境'에서 그 구체적인 내용이 드러나고 있다. 十二□는 열두 명의 여성을 의미하기도 하지만 고전에서 매우 많은 젊은 여성을 가리키는 말로 쓰여왔음을 지적하면서 淸初의 ≪杏花精天≫ 에서 그 용례를 찾고 있다. (整理 崔溶澈) [筆者紹介] □文成, 1941년 출생, 1966년 遼寧大學 졸업, 1981년이후 遼寧大學교수, 전공은 明淸소설과 滿洲族문학연구, 著書에 ≪淸代文學論橋≫, ≪滿族文學精華≫, ≪近現代紅學集思≫등이 있음.

Continuous-Time Low-pass Sigma Delta with Voltage-Controlled Oscillator and Noise Divider of Spread Spectrum Clock Generator for Bio-system Applications

Wen-Cheng Lai 제어로봇시스템학회 2021 제어로봇시스템학회 국제학술대회 논문집 Vol.2021 No.10

The proposed spread-spectrum clock generator (SSCG) was to improve the jitter signal integrity of eye diagram from the experimental result was compared on system on chip (SOC). The proposed fully integrated circuit consists of continuous-time low-pass sigma delta (Σ-△) analog to digital converter (ADC), voltage control oscillator (VCO), current-reused LC injection locked frequency divider by 8 (ILFD ÷8) as linear mixers and Gm-C loop filter. Ultimately, this design successfully reduced radio frequency interference (RFI) with a smaller spreading ratio as compared with existing design to achieve optimal signal integrity and implemented in Bio-systems of control wireless systems.

Dispersive and rheological properties of Mg-PSZ feedstocks for precision powder injection molding

Wen-Cheng J. Wei,Rong-Yuan Wu 한양대학교 세라믹연구소 2004 Journal of Ceramic Processing Research Vol.5 No.3

The effects of various surfactants on the dispersive and rheological properties of ZrO2 and MgO mixtures in heptane, melt wax and feedstocks were investigated. Several properties of the mixtures were tested to reveal the stabilization in various non-polar binder systems. The feedstocks which contained the surfactant (FP1) with an ester function group produced the highest critical solid loading (56.6 vol%) and least dimensional variation (<0.07%) among five formulations, either choosing from SA, FP1 or NZ38 surfactant. The sintered PSZ (partially stabilized zirconia) samples could reach 99% theoretical density (T.D.) and an average fracture strength of 345 MPa. Dual surfactants, containing FP1 and SA, were also tested, resulting in feedstocks better in rheological properties and homogeneity. The surfactant FP1 apparently improved the homogeneity of the powders in the feedstocks, and consequently refined the properties of parts in the stages of injection molding and sintering.

Investigation into Shearing Behaviour of Loess-Post Harvest Waste Mixture

( Wen-chieh Cheng ),( Lin Wang ),( Zhong-fei Xue ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2

Loess-PHW (post-harvest waste) mixture is easily accessible in Chinese Loess Plateau and widely applied to construction of the residential houses that have been utilised over decades under freeze-thaw cycles. However, the shearing behaviour of the loess-PHW mixture is rarely discussed by published literatures. Thus, a consensus has not yet reached. This study investigates the shearing behaviour of the loess-PHW mixture using small-scale and large-scale direct shear (SSDS and LSDS) tests. Four typical shear stress versus horizontal displacement curves are recognised where one is featured with strain-softening shape and the other three strain-hardening shape. Two out of the three curves with strainhardening shape present a gradual increase in the shear stress at additional displacement and larger displacement respectively. Comparisons of the shear strength measured in SSDS and LSDS are made indicating that the scatter exists between SSDS and LSDS. The effect of the PHW dosage on an improvement of the shear strength is assessed to determine the optimal dosage. The improvement is initiated with the added PHW and developed with the aid of adding more water to specimen. Particle-box interaction behaviour is also assessed, and the findings satisfactorily address the gradual increase in the shear stress observed in the strain-hardening curves. The improved shearing behaviour exhibits a great potential of application to sustainable development of urban areas.

Biosynthesis of 3-Hydroxy-5-Methyl-O-Methyltyrosine in the Saframycin/Safracin Biosynthetic Pathway

Cheng Yu Fu,Man Cheng Tang,Chao Peng,Lei Li,Yan Ling He,Wen Liu,Gong Li Tang 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.5

Enhancement of the Polar Coercive Force for Annealed Co/Ir(111) Ultrathin Films

Wen-Yuan Chan,Du-Cheng Tsai,Wei-Hsiang Chen,Cheng-Hsun-Tony Chang,Jyh-Shen Tsay 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.12

The alloy formation and the magnetic properties of Co/Ir(111) ultrathin films have been investigated. As the temperature is increased above 400 K, interdiffusion of Co and the Ir substrate occurs. Due to a compositional change in the surface layers, the polar coercive force is greatly enhanced. At temperatures above 600 K, magnetic hysteresis appears only in the polar configuration. Thisshows that the easy axis of the magnetization of Co/Ir(111) may be stabilized in the direction ofthe surface normal by thermal-annealing treatments. From systematic investigations of Co/Ir(111)ultrathin films thinner than 4 monolayers, a magnetic phase diagram has been established. Accordingto the compositional changes and related magnetic properties, the phase diagram can beseparated into three regions. In region I at temperatures below 400 K, Co films are ferromagnetic. In region II where atomic interdiffusion occurs in the surface layers, an enhanced polar coerciveforce is observed. The phase transition from phase I to II is related to the interdiffusion betweenthe Co overlayer and the iridium substrate. In region III for low coverage or at high temperatures,a nonferromagnetic behavior is observed. The phase transition from phase II to III is mainly dueto the reduced atomic percent of cobalt in the Co-Ir alloy and to the lowered Curie temperaturecaused by a reduction in the thickness of the magnetic layers.

十九世紀來台傳敎士的海洋經驗與書寫

( Cheng Wen Wang ) 제주대학교 탐라문화연구원 2013 탐라문화 Vol.0 No.42

Although researchers have given attention to the history of 19th century Taiwan, but the historiography of Protestant missionaries in Taiwan during the 19 century is a realm that has been more or less neglected to present. The purpose of this article is to examine research through the records of the nineteenth-century missionary to Taiwan to understand the ocean experience of missionaries, and the writing of the ocean. In an overall view, the ocean writing of missionaries provides us a new look to observe Taiwan from an external perspective and helps us to know, what is the western knowledge of the Orient.

Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells

( Cheng Wen Yao ),( Kyoung Ah Kang ),( Mei Jing Piao ),( Yea Seong Ryu ),( Pattage Madushan Dilhara Jayatissa Fernando ),( Min Chang Oh ),( Jeong Eon Park ),( Kristina Shilnikova ),( Soo-young Na ),( 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.3

We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5- FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.