Comparing of backfat microRNAomes of Landrace and Neijiang pig by high-throughput sequencing

Yanyue Li 한국유전학회 2021 Genes & Genomics Vol.43 No.5

Background MicroRNAs (miRNAs) could regulate the expression of target genes and play important roles in modulation of various metabolic processes. Nevertheless, little is known about the backfat microRNAome (miRNAome) of the Neijiang pig. Objectives The primary objective of this study was to analyse miRNAomes of Landrace and Neijiang pig backfat (LPB and NPB resp.). Furthermore, investigating diferentially expressed miRNAs participating in lipid metabolism and mining potential biomarker for Neijiang pig breeding. Methods Here we used the Landrace pig with diferent metabolic characteristics as a control to analyse the Neijiang pigspecifc backfat miRNAome. A comprehensive analysis of miRNAomes was performed by deep sequencing. Results Small RNA sequencing identifed 326 unique miRNAs, 280 were co-expressed in both libraries. Only 11 and 35 miRNAs were specifcally expressed in LPB and NPB respectively. Sixty seven diferentially expressed miRNAs were identifed by IDEG6. MiR-1-3p were identifed that may participate in lipid metabolism. Furthermore, qPCR results revealed that lower expression of miR-1-3p in NPB could increase the expression of LXRα, which is an enzyme important for the synthesis and accumulation of lipid. The double luciferase report experiment suggested that LXRα was the direct target gene of miR-1-3p. In short, miR-1-3p could modulate the synthesis and accumulation of lipid by target LXRα. It may be a potential marker for pig breeding. Conclusion Our investigation has delineated the diferent miRNAs expression patterns of LPB and NPB, which may help understand the regulatory mechanisms of miRNAs in the lipid metabolism, and provide potential biomarkers for Neijiang pig breeding.

One-pot Process of 2'-Deoxyguanylic Acid Catalyzed by a Multi-enzyme System

Yanyu Li,Qingbao Ding,Ling Ou,Yahui Qian,Jiao Zhang 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1

2'-Deoxyguanylic acid (deoxyguanosine-5'-monophosphate, dGMP) is a substance required by livingcells that is used extensively in reagents, fine chemicalsand other industrial fields. Traditionally, dGMP is separatedfrom DNA degradation products, which is low-yieldingand time-consuming. Herein, we investigated a novel, onepotmulti-enzymatic cascade reaction to produce dGMP. This reaction involved purine nucleoside phosphorylase(PNPase) and acetate kinase (ACKase) from Escherichiacoli, N-deoxyribosytransferase II (NDT-II) from Lactobacillusdelbrueckii and deoxyguanosine kinase (dGKase) fromBacillus subtilis. During the reaction, the initial guanosinesubstrate was cleaved into guanine and ribose-1-phosphateby PNPase. Then, deoxyguanosine (dGR) was subsequentlyproduced from a reaction between guanine and thymidinecatalysed by NDT-II. Finally, the intermediate dGR wasphosphorylated to dGMP by dGKase and a cytidinetriphosphate (CTP) regeneration system that utilised acetylphosphate via ACKase. A very small amount of CTP wasadded because CTP regeneration was efficient to transfer aphosphate group from acetyl phosphate to dGR. After 12 hof incubation, a maximal dGMP yield of up to 76% wasobtained based on the addition of 5 mM guanosine and 5mM thymidine.

Low-density polyethylene-derived carbon nanotubes from express packaging bags waste as electrode material for supercapacitors

Yanyu Chen,Xiao Wang,Huaijun Lin,Florian Vogel,Wei Li,Lin Cao,Zhidan Lin,Peng Zhang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.119 No.-

A simple and efficient method for disposing of waste low-density polyethylene (LDPE) express packagingbags is proposed in this paper as a way to minimize waste and address the energy shortage at the sametime. In addition to following the production of carbon nanotubes (CNTs) derived from LDPE, the applicationof CNTs as electrodes for supercapacitors was attempted. Waste LDPE bags were pyrolyzed andcarbonized with catalyst using a dual-temperature reaction system, a designed 450 C pyrolysis temperatureaccompany with different carbonization temperatures (650 C, 700 C, 750 C, 800 C, 850 C), andtreatment durations (0 h, 2 h, 4 h, 6 h) were investigated. As determined by FE-SEM and FE-TEM, the CNTssynthesized at 750 C showed a finer tube diameter, much more uniform distribution, bamboo-like structure,as well as the highest yield of 41.9 %, and carbon conversion of 61.2 %. As being charged and discharged10,000 times, the electrode remained a 93.16 % capacitance and a 92.85 % Coulomb efficiency. A dual-temperature reaction system could be utilized to recycle waste LDPE efficiently, and the LDPEderivedCNTs could be applied for supercapacitors.

Disease Course and Outcomes in Patients With the Limited Form of Neuromyelitis Optica Spectrum Disorders and Negative AQP4-IgG Serology at Disease Onset: A Prospective Cohort Study

Xiao-Dong Li,Jing Zhou,Rui Li,Bingjun Zhang,Yuge Wang,Xiaonan Zhong,Yaqing Shu,Yanyu Chang,Wei Qiu 대한신경과학회 2022 Journal of Clinical Neurology Vol.18 No.4

Background and Purpose Patients presenting with clinical characteristics that are strongly suggestive of neuromyelitis optica spectrum disorders (NMOSD) have a high risk of developing definite NMOSD in the future. Little is known about the clinical course, treatment, and prognosis of these patients with likely NMOSD at disease onset. Methods This study prospectively recruited and visited 24 patients with the limited form of NMOSD (LF-NMOSD) at disease onset from November 2012 to June 2021. Their demographics, clinical course, longitudinal aquaporin-4 immunoglobulin G (AQP4-IgG) serology, MRI, therapeutic management, and outcome data were collected and analyzed. Results The onset age of the cohort was 38.1±12.0 years (mean±standard deviation). The median disease duration was 73.5 months (interquartile range=44.3–117.0 months), and the follow-up period was 54.2±23.8 months. At the end of the last visit, the final diagnosis was categorized into AQP4-IgG-seronegative NMOSD (n=16, 66.7%), AQP4-IgG-seropositive NMOSD (n=7, 29.2%), or multiple sclerosis (n=1, 4.2%). Seven of the 24 patients (29.2%) experienced conversion to AQP4-IgG seropositivity, and the interval from onset to this serological conversion was 37.9±21.9 months. Isolated/mixed area postrema syndrome (APS) was the predominant onset phenotype (37.5%). The patients with isolated/mixed APS onset showed a predilection for conversion to AQP4-IgG seropositivity. All patients experienced a multiphasic disease course, with immunosuppressive therapy reducing the incidence rates of clinical relapse and residual functional disability. Conclusions Definite NMOSD may be preceded by LF-NMOSD, particularly isolated/ mixed APS. Intensive long-term follow-up and attack-prevention immunotherapeutic management is recommended in patients with LF-NMOSD.

Comparison of stomach microRNA transcriptomes of Tibetan and Yorkshire pigs by deep sequencing

Wen‑kui Sun,Yanyue Li,Chi Cheng,Yi‑hui Chen,Kai Zeng,Xiaohui Chen,Yiren Gu,Rui Liu,Xuebin Lv,Rong Gao 한국유전학회 2018 Genes & Genomics Vol.40 No.9

MiRNAs regulate the expression of target genes in diverse cellular processes and hence play important roles in different physiological processes, yet little is known about the stomach microRNAome (miRNAome) of the Tibetan pig. The objective of this experiment was to investigate differentially expressed stomach miRNAs participating in digestion. Firstly, we isolated total RNA by Trizol reagent from three Tibetan and three Yorkshire purebred pigs stomach samples at 90-day-old. Secondly, a comprehensive analysis of Tibetan and Yorkshire pig stomach miRNAomes was performed by small RNA sequencing in the Illumina HiSeq 2000 system. Finally, SYBR Green Real-time RT-PCR was performed to validate the differentially expressed miRNAs. We identified 318 unique miRNAs, 260 were co-expressed in both libraries, 17 and 31 miRNAs were specifically expressed in Tibetan and Yorkshire pigs respectively. Fifty six differentially expressed miRNAs were identified by the identifying differentially expressed genes 6 (IDEG6). Kyoto encyclopedia of genes and genomes analysis revealed that some of the differentially expressed miRNAs were associated with protein and fat digestion. Two differentially expressed miRNAs (miR-214-3p and ssc-un39) participating in the digestion of lipid were identified. Additionally, qRT-PCR results suggested that a higher expression of miR-214-3p in the Tibetan pig stomach could lead to relatively lower expression of calcium-dependent phospholipase A2, which is an enzyme important for the digestion of glycerol phospholipid. This study has delineated the different stomach miRNAs expression patterns of Tibetan and Yorkshire pigs, which would help explain the regulatory mechanisms of miRNAs in digestion of Tibetan pigs, and contribute to utilize a the unique digestion merits of Tibetan pig in future porcine hybridization breeding.

Stable Sheet-Beam Transport in Periodic Nonsymmetric Quadrupole Field

Zhanliang Wang,Yubin Gong,Yanyu Wei,Zhaoyun Duan,Huarong Gong,Zhigang Lu,Lingna Yue,Hairong Yin,Jin Xu,Yuanbao Li,Pukun Liu,Gun-Sik Park IEEE 2010 IEEE transactions on plasma science Vol.38 No.1

<P>Stable sheet electron-beam transport is critical for sheet-beam microwave device which is attractive for high-power millimeter wave to terahertz-regime radiation. This paper studies the stable sheet-beam transport in periodic nonsymmetric quadrupole field. First, the conditions for stable round- and sheet-beam transport in periodic magnetic quadrupole field are deduced. In the deduction, we find that the symmetric quadrupole field and the space-charge field of sheet beam are not well matched. In order to settle this problem, we use periodic nonsymmetric quadrupole field instead of periodic symmetric quadrupole field to transport sheet beam. Finally, 3-D PIC simulations verify the conditions for stable sheet-beam transport and show that periodic nonsymmetric quadrupole field is intrinsically well suited for sheet-beam transport.</P>