Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

실용음악 전공 학생들의 발성치료 전후 성대 및 음성 특성 비교

황연신,심미란,김철준,최용석,김상연,최찬호,선동일,Hwang, Yeon Shin,Shim, Mi Ran,Kim, Chul Jun,Choi, Yong Suk,Kim, Sang Yeon,Choi, Chan Ho,Sun, Dong Il 대한후두음성언어의학회 2016 대한후두음성언어의학회지 Vol.27 No.2

Background and Objectives : Many students major in applied music singing have various difficulties in phonation when they sing or perform. But the studies about this cause are lack. The purpose of this study is whether singing voice therapy based on vocal music is effective or not to applied music singing students that have difficulties in phonation. Materials and Methods : Singing voice therapy program had been done to 8 students major in applied music singing during 7 weeks. We did laryngeal stroboscope, perceptual, acoustic and aerodynamic examination before and after singing voice therapy to this students. And we studied post examination results have differences or improvements compared to pre examination results. Results : Four male students don't have effective improvement after singing voice therapy but they shows slight improvement in aerodynamic examination values compared to pre examination values. Four female students shows improvement in aerodynamic examination values and pitch range increasing. Above all, one student's vocal nodules disappeared after singing voice therapy. Conclusion : Singing voice therapy based on music singing is very effective to the students major in applied music singing. Above all, their curriculum in applied music singing should include phonation training based on music singing before training perform and technic practices.

社會福祉政策의 분석 : with respect to medical security in Korea 醫療保障制度를 中心으로

全相京 東亞大學校 大學院 1983 大學院論文集 Vol.7 No.-

Much emphasis had been laid upon social development since the fourth Five-Year Economic Plan. Along with this move to put emphasis upon social development, we are more concerned with social welfare than ever before. There are two views on social welfare: residual conception and institutional one. The residual conception of welfare lends weight to the conception that social welfare serves mainly such function wherein its activities are perceived as necessary only when those who are served by the "normal" institutional channels are unable to benefit, either through personel failings and exceptional needs or when those "normal" channels fail to perform appropriately. On the other hand, the institutional conception of welfare is as a distinct pattern of activities serving not a safety net to catch the pieces after all else has failed, but as an integral and "normal first line function of modern industrial society." Medical security is one of social welfare policies in functional views. And medical secutity consists of medical care and medical insurance. Medical care is a kind of public assistance which is conducted by the government revenue, while medical insurance is a sort of social insurance which aims the dispersion of social risk and income re-distribution. When we say about medical security from here on, we mean it both medical care and medical insurance. We analyze medical security using Gilbert and Specht's framework of social welfare policy. Their framework are as follows: the bases of eligibility of medical security benefits, the kind of medical security benefits, the mode of financing for medical security benefits and the delivery system of medical security benefits. To improve the relevance of this study, we should conduct field survey to distil useful information from the receipients of this policy, the authorities concerned with this policy and medical centers which deliver medical services to recipients. But due to many constraints, we depend upon only literary survey such as the acts concerned with medical security.

기하학적 특징을 이용한 얼굴 특징점 추출 알고리즘

전승철,박성한 漢陽大學校 工學技術硏究所 1998 工學技術論文集 Vol.7 No.1

사람의 얼굴은 일반 객체와는 다르게 정확히 구별되는 특징을 가지고 있지 않다. 따라서 일반적으로 사람 얼굴에 관한 연구에서는 인간이 사람의 얼굴을 볼 때 가장 먼저 인식을 하는 눈, 코, 입을 특징으로 정하고 있다. 이러한 특징은 사람에 따라 다르게 나타나며 주위환경에 영향을 받는다. 따라서 이러한 사람의 특징을 정확히 찾아내는 것이 중요하다. 본 논문에서는 얼굴 특징점의 기하학적 성질을 이용 제약조건을 제시하여 제약조건에 만족하는 객체를 후보객체로 설정하였다, 그리고 눈, 코, 입 각각의 알고리즘을 제안하여 원하는 특징점을 효율적으로 찾아내는 알고리즘을 제안하고 있다. 제안된 알고리즘은 일반적인 사람 얼굴 영상에 대해 빠른 속도로 특징점을 찾아내고 있다. The human faces do not have distinct features unlike other general objects. In general the features defined eyes, nose and mouth which are firstly recognized when human being see the face. These features have different characteristics each human face. Therefor, the exactly finding the human features are very important. In this paper proposed an algorithm finding eyes, nose and mouth using the geometric characteristics of human face. candidate objects are found by the constraints of features. And then each proposed algorithm of eyes, nose and mouth is using for finding exactly objects. The proposed algorithm fast find the features in general human face image.

통계적 학습이론을 이용한 효율적인 데이터 마이닝 전략

전성해 청주대학교 산업과학연구소 2007 産業科學硏究 Vol.24 No.2

Statistical Learning theory(SLT) by Vapink was introduced to overcome the problems of local optima and over-fitting in machine learing algorithms. SLT is consisted of support vector machine(SVM), support vector regression(SVR), and support vector clustering(SVC) for classification, regression, and clustering respectively. So, SLT is a powerful tool for supervised and unsupervised learning at once. There is no algorithm to support supervised and unsupervised tools at a time. But, SLT is able to contribute two learning algorithms. In this paper, we propose an efficient data mining approach using SLT. To verify our work, we use data set from UCI machine learning repository and R-project.

Ethylenediamine-N,N'-di-α-Butyric Acid의 로디움(Ⅲ) 착물

전무진,한창윤,박장우,최승락,박광하 연세대학교 자연과학연구소 1985 學術論文集 Vol.15 No.-

네자리 리간드인 ethylenediamine--N,N'-di-α-butyric acid(eddb)의 디클로로 로디움(Ⅲ)착물을 합성하고 크로마토그래피법으로 분리하였다. Eddb리간드가 로디움(Ⅲ) 이온에 배위될 때 s-cis와 uns-cis기하 이성체가 형성됨이 관찰되었다. 합성된 착물의 구조는 적외선 분광분석법, 핵자기 공명 및 흡수 스펙트럼으로 확인되었다. Dichloro rhodium(Ⅲ) complex of a flexible tetradentate ligand of ethylenediamine-N,N'-di-α-butyric acid(eddb) has been prepared and separated by the chromatographic method. S-cis and uns-cis geometric isomers have been yielded during the preparation of complex. IR, PMR and electronic absorption spectra have been used to characterize the complex.

政府의 意思決定과 便益費用 分析 : 프로젝트 評價를 中心으로 with Special Reference to Public Project Appraisal

全相京 東亞大學校 1981 東亞論叢 Vol.18 No.1

This paper aims to review and systematize the economic criteria for efficient and rational public expenditure, and then examine how the investment criteria system has been developed in Korea. Previously the major concern in government economy was mainly confined to taxes. But as amount of government expenditure increase with the accelerating government's role, there has been arising much need for rational and economically efficient public expenditure. This calls for adequate criteria for economicaly rational resource allocation. And welfare economics developed since 1950 could meet this request. The most widely used method in finding economic rationally of public expenditure is "Benefit-Cost Analysis". It originated from Water Resource Projects of the States in 1930s. And recently PPBS and ZBB requires B-C analysis in budget-making process. The concept of B-C analysis is as follows: "a practical way of accessing the the desirability of projects, where it is important to take a long view(in the sense of looking at repercussions in the future)and a wide view(in the sense of allowing side-effects of many kinds on many persons, industries, regions, etc.), i.e. it implies the enumeration and evaluation of all the relevants costs and benefits." In B-C analysis, we have four criteria for adopting projects: Benefit-Cost Ratio, Net Present Value(NPV), Internal Rate of Return(IRR), and Payback Period. The B-C Ratio was once the most widely used, but nowadays many authorities in this field recommend the use of NPV. It must be true that B-C analysis gives us a criterion for rational resource allocation. But, because it is a kind of model to simplify the complex ecnomic reality in order to find an adequate criterion, it also has many restrictions. Such restrictions come from the following factors: difficulties of the adequate choice of discount rate, the consideration of all possible externalities, and the correct estimate of shadow price. In Korea, Economic Planning Board(EPB)has the right of budget-making. The refore EPB has justly the responsibility to establish and institutionalize the public project evaluation system. Therefore since 1976, EPB has established one Bureau which consists of four Sections and takes exclusive responsibility of project evaluation. In this paper, only the general scheme of B-C analysis was introduced, plus the the examination of the Korean public project evaluation system. But in the next study, we should give much more emphasis on one specific field: discount rate, shadow price and how to quantify the externalities. And it is also desirable to execute a case study with a certain already finished public project in order to get a pedagogic lesson.

공정 조절에서 이상원인의 제거 방법에 대하여

전상표 남서울대학교 2005 남서울대학교 논문집 Vol.11 No.2

Assignable causes producing temporary deviation from the underlying system can influence on process adjustment and process monitoring in dynamic feedback control system. In this paper, Chapter Ⅱ will summarize the methods of readjusting unadjusted disturbance when assignable causes occur in dynamic process adjustment, and of removing the causes of assignable causes.

민영화 시대의 은행 지배구조화 감독정책

전성인 홍익대학교 경제연구소 2001 經濟硏究 Vol.16 No.-

한국의 물가안정목표제 운용경험과 정책과제

전성인 弘益大學校 經濟硏究所 2005 經濟硏究 Vol.20 No.-