Radiotherapy combined with immunotherapy could improve the immune infiltration of melanoma in mice and enhance the abscopal effect

Yufeng Zheng(Yufeng Zheng),Xue Liu(Xue Liu),Na Li(Na Li),Aimei Zhao(Aimei Zhao),Zhiqiang Sun(Zhiqiang Sun),Meihua Wang(Meihua Wang),Judong Luo(Judong Luo) 대한방사선종양학회 2023 Radiation Oncology Journal Vol.41 No.2

Purpose: To analyze the gene mutation, immune infiltration and tumor growth of primary tumor and distant tumor under different treatment modes. Materials and Methods: Twenty B16 murine melanoma cells were injected subcutaneously into the of both sides of the thigh, simulating a primary tumor and a secondary tumor impacted by the abscopal effect, respectively. They were divided into blank control group, immunotherapy group, radiotherapy group, and radiotherapy combined immunotherapy group. During this period, tumor volume was measured, and RNA sequencing was performed on tumor samples after the test. R software was used to analyze differentially expressed genes, functional enrichment, and immune infiltration. Results: We found that any treatment mode could cause changes in differentially expressed genes, especially the combination treatment. The different therapeutic effects might be caused by gene expression. In addition, the proportions of infiltrating immune cells in the irradiated and abscopal tumors were different. In the combination treatment group, T-cell infiltration in the irradiated site was the most obvious. In the immunotherapy group, CD8+ T-cell infiltration in the abscopal tumor site was obvious, but immunotherapy alone might have a poor prognosis. Whether the irradiated or abscopal tumor was evaluated, radiotherapy combined with anti-programmed cell death protein 1 (anti-PD-1) therapy produced the most obvious tumor control and might have a positive impact on prognosis. Conclusion: Combination therapy not only improves the immune microenvironment but may also have a positive impact on prognosis.

A Braking Force Distribution Strategy for Four-in-Wheel-Motor-Driven Electric Vehicles on Roads with Different Friction Coefficients

Lian Yufeng,Liu Shuaishi,Zhongbo Sun,Liu Keping,Nie Zhigen,Tian Chongwen 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.4

This paper presents an active collision avoidance system based on a braking force distribution strategy for four-in-wheel-motor-driven electric vehicles (FIWMD-EVs) on roads with different friction coefficients. There are three major contributions in the proposed braking force distribution strategy. Firstly, the braking force distribution strategy based on constrained regenerative braking strength continuity (CRBSC) is further improved, and its general analytic expressions are derived. It provides the theoretical basis of braking force distribution between front and rear wheels. Secondly, the braking forces between front and rear wheels can be redistributed by considering power demand efficiency (PDE) to protect energy storage system from overcharge. Finally, the braking forces between left and right wheels can be distributed with different adhesion coefficients to adapt to complex roads. Simulations using rapid control prototyping (RCP) and hardware-in-the-loop (HIL) simulator are performed to demonstrate the effectiveness of control scheme and adaptability of the active collision avoidance system based on the proposed braking force distribution strategy on complex roads.

Notch ligand Jagged1 promotes mesenchymal stromal cell-based cartilage repair

Junkui Sun,Zhengliang Luo,Guangxi Wang,Yuping Wang,Yisheng Wang,Margaret Olmedo,Massimo Max Morandi,Shane Barton,Christopher G. Kevil,Bing Shu,Xifu Shang,Yufeng Dong 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Placenta-derived mesenchymal stromal cells (PMSCs) provide a promising cell source for tissue regeneration. However, rapid induction of PMSC chondrogenic differentiation during therapeutic transplantation remains extremely challenging. Here we undertook a study to determine if Notch inhibition by soluble Jagged1 (JAG1) peptides could be utilized to accelerate PMSC-induced cartilage regeneration in a mouse post-traumatic osteoarthritis (PTOA) model. Our results showed that treatment of PMSCs with soluble JAG1 significantly enhanced chondrogenesis in culture as shown by increased alcian blue staining and decreased Notch target Hes1 expression when compared to those in lgG-treated control cells. Importantly, significantly enhanced cartilage formation and decreased joint inflammation were observed when JAG1-treated PMSCs were injected into mouse PTOA knee joints. Finally, in vivo cell tracing showed that more JAG1-treated PMSCs remained in knee joint tissues and that JAG1-treated PMSCs exhibited greater PMSC chondrogenic differentiation than lgG-treated control PMSCs at 4 weeks after injection. These data indicate that transient Notch inhibition by soluble JAG1 could be used to enhance PMSC survival and chondrogenic differentiation, thereby increasing the therapeutic potential of PMSCs for cartilage regeneration.

PHYSIOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF THREE SELENIUM-RICH ALGAE AND ANTITUMOR ACTIVITY BY SE-ULVA FASCIATA

Xian Sun,Yu Zhong,Ya Zuo,Yufeng Yang 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5

Bacillus daqingensis sp. nov., a Halophilic, Alkaliphilic Bacterium Isolated from Saline-Sodic Soil in Daqing, China

ShuangWang,Lei Sun,DanWei,Baoku Zhou,Junzheng Zhang,XuejiaGu,Lei Zhang,Ying Liu,Yidan Li,Wei Guo,Shuang Jiang,Yaqing Pan,Yufeng Wang 한국미생물학회 2014 The journal of microbiology Vol.52 No.7

An alkaliphilic, moderately halophilic, bacterium, designated strain X10-1T, was isolated from saline-alkaline soil in Daqing, Heilongjiang Province, China. Strain X10-1T was determined to be a Gram-positive aerobe with rod-shaped cells. The isolate was catalase-positive, oxidase-negative, non-motile, and capable of growth at salinities of 0–16% (w/v) NaCl (optimum, 3%). The pH range for growth was 7.5–11.0 (optimum, pH 10.0). The genomic DNA G+C content was 47.7 mol%. Its major isoprenoid quinone was MK-7 and its cellular fatty acid profile mainly consisted of anteiso-C15:0, anteiso-C17:0, iso-C15:0, C16:0, and iso-C16:0. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences showed that X10-1T is a member of the genus Bacillus, being most closely related to B. saliphilus DSM15402T (97.8% similarity) and B. agaradhaerens DSM 8721T (96.2%). DNA-DNA relatedness to the type strains of these species was less than 40%. On the basis of the phylogenetic, physiological, and biochemical data, strain X10-1T represents a novel species of the genus Bacillus, for which the name Bacillus daqingensis sp. nov. is proposed. The type strain is X10-1T (=NBRC 109404T =CGMCC 1.12295T).

Effect of Mg12Nd and Mg3Nd On the Corrosion Behavior of Mg‒3 wt%Nd Alloy in Saline Solution

Kaihua Yuan,Jianfeng Wang,Yufeng Sun,Shijie Zhu,Hairong Liu,Shaokang Guan 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.5

In this paper, the precipitates of the Mg‒3 wt%Nd alloy in the as-cast state and after heat treatment at 540 ℃ for 12 h wereinvestigated and related to the corrosion behavior in saline solution. For the as-cast specimen, the network and sphericalβ-phases (Mg12Nd) act as the anodes relative to Mg matrix at the initial stage of immersion in saline solution. Withincreasing the immersion time, the intergranular corrosion caused by the preferential dissolution of the reticular β-phaseleads to the detachment of Mg fragments. For the heat-treated specimen, the plate-shaped β1-phases (Mg3Nd) distributedevenly in the grain interior act as the micro-cathodes. The corrosion pits are prone to extend horizontally and connectedeach other, resulting in an uniform corrosion status and a relatively lower corrosion rate. This result is helpful for developingthe biomedical Mg‒Nd base alloys with a good degradation property by adjusting the precipitation of intermetallics.

Stacking-fault energy, mechanical twinning and strain hardening of Fe-18Mn-0.6C-(0, 1.5)Al twinning-induced plasticity steels during friction stir welding

Lee, Seung-Joon,Sun, Yufeng,Fujii, Hidetoshi Elsevier 2018 Acta materialia Vol.148 No.-

<P><B>Abstract</B></P> <P>The effect of friction stir welding (FSW) on the microstructure, stacking-fault energy (SFE) and strain hardening rate (SHR) of Fe-18Mn-0.6C-(0 and 1.5)Al (wt.%) twinning-induced plasticity steels using three welding speeds (50, 100 and 200 mm min<SUP>−1</SUP>) was investigated. The yield strength of the FSWed 0Al and 1.5Al steels improved due to both grain boundary strengthening by grain refinement and dislocation hardening by the introduction of dislocations with an increase in the welding speed. Their SHR with three stages and without the yield drop increased due to the active mechanical twinning and the introduction of dislocations during the FSW when the welding speed was increased. Among the 0Al steels, 0Al-200 steel with a fine grain exhibited more active twinning than the coarse-grained specimen (0Al-50), which is contrast to the 1.5Al steel. Regardless of the specimens, the slight increase in the SFE, which was attributed to both the shear strain energy caused by the introduction of dislocations and the excess free energy by the grain refinement during the FSW, leads to an increase in the critical twinning stress (<I>σ</I> <SUB> <I>tw</I> </SUB>). Despite the fine grain of the 0Al steel, the origin of its active twinning was the highly increased yield strength relative to <I>σ</I> <SUB> <I>tw</I> </SUB>, and the promoted dislocation interactions, giving rise to an increase in the number of sites at which twin nucleation occurred.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Friction stir welding of multi-walled carbon nanotubes reinforced Al matrix composites

Lee, Seung-Joon,Shin, Se Eun,Sun, Yufeng,Fujii, Hidetoshi,Park, Yongbum Elsevier 2018 Materials characterization Vol.145 No.-

<P><B>Abstract</B></P> <P>In this study, we prepared composites between Al and multi-walled carbon nanotubes (MWCNTs) using the friction stir welding (FSW) method and investigated their microstructural evolution and mechanical properties by controlling the amount of MWCNT (1 and 3 vol%) and the plunging load (400 and 600 kg) used. After the FSW process, MWCNT showed slight coarsening of grain with random shear textures and accumulated dislocations; the differences obtained by varying the plunging load and MWCNT amount were insignificant. The friction-stir welded composite obtained using 3 vol% MWCNT and a plunging load of 600 kg possessed two-times better balance between strength and ductility than the base metal, due to the presence of both MWCNTs and the partially decomposed Al<SUB>4</SUB>C<SUB>3</SUB> in the composite.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Al/MWCNT composites were successfully welded using friction stir welding. </LI> <LI> After FSW, the composites possessed an excellent balance of mechanical properties. </LI> <LI> High tensile strength and elongation over BM owing to MWCNTs after FSW. </LI> <LI> Microstructures were investigated according to MWCNT contents and FSW conditions. </LI> </UL> </P>

Cross-coupled Transistor Load Structure in Differential Pairs

Wenqin Xu,Qiang Li,Jing Wang,Yufeng Sun,Yasuaki Inoue 대한전자공학회 2015 ITC-CSCC :International Technical Conference on Ci Vol.2015 No.6

The cross-coupled transistor structure has been widely utilized in LSI circuits. This paper presents a cross-coupled transistor load structure for differential amplifiers and the performance of the structure is analyzed. The differential amplifier composed of the cross-coupled transistor load structure and the differential pair is verified by circuit simulation and the simulation results indicate that the structure can improve the performances of the differential amplifier in several parameters including the gain and the quiescent current.

Meta-analysis of randomized controlled trials on the efficacy of daikenchuto on improving intestinal dysfunction after abdominal surgery

Lei Zhang,Yusheng Cheng,Huizi Li,Yufeng Zhou,Bo Sun,Leibo Xu 대한외과학회 2018 Annals of Surgical Treatment and Research(ASRT) Vol.95 No.1

Purpose: Intestinal dysfunction is one of the most common complications in patients after abdominal surgery. Daikenchuto (DKT), a traditional herbal medicine, is recently employed to improve postoperative intestinal dysfunction. The aim of this meta-analysis was to assess the efficacy of DKT in improving intestinal dysfunction after abdominal surgery. Methods: PubMed, Embase, and the Cochrane library were systematically searched to identify randomized controlled trails (RCTs) in adult patients undergoing abdominal surgery, who were randomly distributed to administrate DKT and placebo. The primary outcomes included the time to first postoperative flatus or bowel movement. We used random-effects models to calculate summary mean differences (MDs) with 95% confidence intervals (CIs). Results: Nine RCTs totaling 1,212 patients (618 in DKT, 594 in control group) were included in our study. Compared with control group, DKT can effectively improve postoperative intestinal dysfunction by shortening the time to first postoperative flatus (MD, -0.41; 95% confidence interval [CI], -0.66 to -0.16; P = 0.001) with significant heterogeneity (I<SUP>2</SUP> = 71%, P = 0.004), and bowel movement (MD, -0.65; 95% CI, -0.97 to -0.32; P < 0.001) without significant heterogeneity (I<SUP>2</SUP> = 40%, P = 0.14). Sensitivity analyses by indication of surgery and type of surgery yielded similar results. Conclusion: These data provide limited evidence that DKT shows efficacy on improving intestinal dysfunction after abdominal surgery. However, the results should be interpreted cautiously, due to the heterogeneity of the studies included. Thus, the efficacy of DKT on improving postoperative intestinal dysfunction warrants further investigation.