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Shin-Gu Kang,Mian Sayeed Hassan,Bon-Il Ku,Wan-Gyu Sang,Min-Kyu Choi,Young-Doo Kim,Hong-Kyu Park,M. Khalequzzaman A. Chowdhury,Bo-Kyeong Kim,Jeom-Ho Lee 韓國作物學會 2013 Korean journal of crop science Vol.58 No.3
A field study was conducted to understand nitrogen use efficiency of high yielding Japonica rice varieties under three levels of nitrogen fertilizer (90, 150 and 210 kg N ha -1 ) in Iksan, Korea. Two high yielding rice varieties, Boramchan and Deuraechan, and an control variety, Dongjin2, were grown in fine silty paddy. Nitrogen use efficiencies (NUE) were 83.3, 56.3, and 41.2 in 90, 150, and 210 kg N ha -1 fertilizer level, respectively. Total nitrogen uptake varied significantly among nitrogen levels and varieties. Variety Dongjin2 showed the highest nitrogen uptake efficiency (NUpE), while Boramchan and Deuraechan showed higher nitrogen utilization efficiency (NUtE). However, Nitrogen harvest index (NHI) was higher in Boramchan (0.58) than Deuraechan (0.57) and Dongjin2 (0.53). Rough rice yield showed linear relationship with total nitrogen uptake (R 2 =0.72) within the range of nitrogen treatments. Boramchan produced significantly higher rough rice yield (8546 kg ha -1 ) which mainly due to higher number of panicles per m 2 compared to Deuraechan (7714 kg ha -1 ). Deuraechan showed higher number of spikelets per panicle, but showed lower yield due to lower number of panicle per m 2 . Rice varieties showed different nitrogen uptake ability and NUE at different nitrogen level. Plant breeders and agronomist should take advantage of the significant variations and relationships among grain yield, NUpE, and NUE.
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Salmonella typhi Salmonella typhimurium 및 Salmonella enteritidis의 항균제 감수성(1997)
신영학,유정식,김기상,정동준,오경수,이점규,이상원,이근영,박미선,이복권,김호훈 대한화학요법학회 1998 대한화학요법학회지 Vol.16 No.3
목적 : 국내에서 토착화 발생하여 공중보건학상 중요관리 대상 병원체인 장티푸스균과 최근 범세계적 유행추세를 보이고 있으며 우리나라에서도 급속히 증가추세를 보이고 있는 세균성 식중독의 원인균인 S. Enteritidis 및 S. Typhimurium을 대상으로 시험관내 항균제 감수성시험을 실시하여 항균제 내성양상을 파악하고자 하였다. 방법 : 1997년 1월부터 12월사이 전국 16개 시도 보건 환경 연구원 및 일부 종합병원으로부터 수집 동정한 장티푸스균 79주, S. Enteritidis 114주 및 S. Typhimurium 83주를 대상으로 미량 액체 희석 법으로 최소 발육 억제농도를 측정하고, break Point를 적용 내성양상을 분석하였다. 사용한 항균제는 β-lactam계 항균제로 ampicillin, amoxacillin, carbenicillin, cefoxitin, cephalothin, ceftriaxone, timentin(ticarcillin/clavulanic acid)을, aminoglycoside계로 streptomycin, amikacin, gentamicin, kanamycin을, quinolone계로 ciprofloxacin을 기타 tetracycline, nalidixic acid, chloramphenicol, trimethoprim, trim e th oprim/sulfamethoxazole등 총 17종 이었다. 결과 : 17종의 항균제중 1 종 이상의 항균제에 내성을 지니고 있는 균주가 장티푸스균 79주 중 15주 (19.0%), S. Enteritidis 114주중 14주(12.3%) 그리고 S. Typhimurium 83주 중 67주(80.7%) 였다. 시험에 공시된 살모넬라균종에 대해 전체적으로 tetracycline 및 페니린계의 항생제에 대한 내성균 검출빈도가 높았다. 다만 장티프스균의 경우 chloramphenicol 내성균주가 12주(15.2%) 검출되었으며, S. Typhimurium은 Streptomycin 내성 균주가 37주 (44.5%) 검출되어 높은 내성율을 보였다. 3균종 모두에서 ceftriaxone 및 ciprofloxacine 에 내성을 보이지 않았다. 다제내성 양성을 보면 장티프스균은 한가지 이상의 균제에 대해 내성을 나타낼 15주 중 10균주가 tetracycline, chloramphenicol, ampicillin, 및 trimethoprim등 4종 이상의 항균제에 대해 내성을 갖는 다제 내성 균주로 전체 내성균의 66.7%를 차치하였고 특히 9종의 항균제에 내성을 갖는 균이 3주, 8종의 항균제에 내성을 갖는 균이 5주나 검출되었다. S. Enteritidis균의 다계 내성 양상은 gentamycin을 비롯한 8종의 항균제에 내성을 지닌 균주가 1주 있었으며 4종이상의 항균제에 내성을 갖는 다제내성균이 6주로 내성균의 42.8%를 차지하였다. 주로 tetracycline과 페니실린계 항생제에 동시에 내성을 갖는 것으로 나타났다. S. Typhimurium의 다제내성양상은 tetracycline 및 streptomycin 등 2종의 항생제에 내성을 가진 형이 25주로 가장 많았으며, tetracycline 과 페니실린계 항생제에 동시에 내성을 나타내는 경우도 많았다. 또한 4종 이상의 항균제에 내성을 갖는 다제 내성균이 29주로 내성균의 43.3%를 차지했으며, S. Typhimurium DT104 주와 유사한 항균제 내성 양상을 나타내는 균주도 확인되었다. 결론 : 국내에서 분리된 장티푸스균, S. Enteritidis 및 S. Typhimurium 감수성 시험 결과 항균제 내성이 현재까지 일반적인 항균제에 국한되어 있으나 S. Typhimurium DT104주와 유사한 항균제 내성 양상을 보이는 균주가 확인되었고 과거에는 분리 보고가 흔하지 않았던 4종이상의 항균제에 내성을 지닌 장티푸스균이 다수 검출되었으므로 항균치료제 선택에 신중을 기함은 물론 내성균주 감시를 체계적이고 지속적으로 실시하여야 할 것으로 사료된다. Background : Salmonella Typhi, an endemic in Korea, S. Enteritidis and S. Typhimurium, major organisms of bacterial food poisoning, which have been responsible for numerous outbreak in the m7orld including Korea currently are important organisms to be in control. At this point, to understand current antimicrobial resistance of the three Salmonella serovars in Korea, we conducted in vitro susceptibility studies to 17 antimicrobial agents. Methods : We tested antimicrobial susceptibility by a microbroth dilution method. The target organisms are 79 isolates of S. Typhi, 114 isolates of S. Enteritidis, and 83 isolates of S. Typhimurium collected on January through December 1997 in Korea. Results : The resistance rates to one or more antimicrobial agents are 19.0%(15/79 isolates) for S. Typhi, 12.3% (14/114 isolates) for S. Enteritidis, and 80.7% (67/83 isolates) for S. Typhimurium. Grossly, the organisms' resistance rates to tetracycline and penicillins are higher than that of other agents with two exceptions : 15.2% to chloramphenicol (12/79 isolates) for S. Typhi and 44.6% to streptomycin (37/83 isolates) for S. Typhimurium. Multi-drug resistant (MDR) strains to four or more antimicrobial agents among the resistant organisms are quite high: 66.7% (10/15 isolates), 42.8% (6/14 isolates), and 43.3% (29/67 isolate) for S. Typhi, S. Enteritidis, and S. Typhimurim, respectively. Conclusion : From this investigatron, we found that the MDR strains of S. Typhi, S. Enteritidis, and S. Typhimurim have increased than previous studies. It implies that limitation of unnecessary use of drugs is very important. in order to maintain the efficacy of drugs.
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Kyung-Ho Kang,Ji-Ung Jeong,Hyang-Mi Park,Myeong-Gi Kim,Yeong-Seop Shin,Chang-In Yang,Jae-Gi Jang,Eug-Geun Ahn,Jeom-Ho Lee 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Breeding the whole crop siliage rice cultivar basically requires priority in high biomass and grain yield and tolerance biotic and abiotic stresses. In this context, AA genome wild rices are potential resources to broaden genetic variability for whole crop silage cultivar. We have developed several introgression populations from crosses with three AA genome wild rices, O.glaberrima, O.rufipogon, and O.longistamina. Among these populations, the three BC4F8 near isogenic lines (NILs) derived from a cross of Milyang 23 and O.glaberrima(acc. 101154) could produce the high-yielding lines surpassing Milyang 23 by 20% in grain yield. Introgression lines from crosses with 4 accessions of O.rufipogon with japonica rices, Hwaseongbyeo and Ilpumbyeo had huge agronomic variability and very tall and vigorous lines possessing culm length of 125 ∼ 187cm were selected as promising potential parents to improve biomass of existing cultivars. These selections with high yield potential and biomass will be crossed to create another gene pool to combine high yield and biomass and anther culture breeding could be useful to develop genetically fixed F2 lines with both traits.
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호남평야지 청보리-벼 이모작에서 벼 폿트묘 적정 재식밀도 구명
강신구 ( Shin Gu Kang ),김영두 ( Young Doo Kim ),구본일 ( Bon Il Ku ),상완규 ( Wan Gyu Sang ),이민희 ( Min Hee Lee ),박홍규 ( Hong Kyu Park ),이점호 ( Jeom Ho Lee ) 전북대학교 농업과학기술연구소 2014 농업생명과학연구 Vol.45 No.2
본 시험은 호남평야지에서 청보리-벼 이모작 재배시 벼폿트묘에 알맞은 적정 재식밀도를 구명하기 위하여 수행하였다. 관행산파묘와 폿트묘를 이용하여 재식밀도별 생육상황을 보면 경직경은 관계없이 관행보다 폿트묘에서 굵었고, 출수기는 폿트묘 이앙구에서 50주~70주/3.3m2가 관행보다 2일, 80주/3.3m2가 관행보다 1일 정도 빨랐다. 간장은 관행산파묘보다 폿트묘의 간장이 더 컸고, 재식밀도별로는 재식밀도가 낮을수록 간장이 더 크게 나타났으나, 이삭길이는 재식밀도별로 유의한 차이를 보이지 않았다. 이삭수는 재식밀도가 높아질수록 단위면적당 이삭수가 많아지는 경향이었다. 등숙비율 및 천립중은 육묘방법 및 재식밀도 사이에 유의한 차이가 없었다. 백미수량은 재식밀도별로 보면 폿트묘 60주/3.3m2가 543kg/10a로 관행 518kg/10a 보다5% 증수하였다. 완전미 비율은 폿트묘 80주/3.3m2 이앙구에서 다소 낮았고, 육묘방법간에 차이는 없었으나 재식밀도 간에는 다소 차이가 있었던 반면, 아밀로스 함량은 재식밀도 간에 유의한 차이가 없었다. This experiment was carried out to investigate the optimum planting density for rice pot seedling cultivation by machine transplanting in forage barley-rice double cropping system in Honam plain area. Field experiment was conducted at research field of NICS in Iksan in 2012 and 2013. Rice seedling of Chinnongbyeo (mid-late maturing) was raised for 30 days in pot-seedling tray (448 holes) and conventional tray, and transplanted on June 10 in both years. Planting densities of pot seedling were 50, 60, 70, and 80 hills/3.3m2. Conventional tray seedling was transplanted as control at a planting density of 80 hills/3.3m2 on June 10. Tiller number per m was increased as planting density increasing, but stem diameter was decreased. Heading dates of pot seedling plots were August 19 except 80 hills/3.3m2, which were 2 days earlier than the control. Culm length, number of panicle, panicle length, and ripening grain ratio were higher in pot seedling compared to the controls, but 1000-grain weight showed no significant difference. Milled rice yield was significantly different between planting densities. The highest grain yield was 543kg/10a in 60 hills/3.3m2 of pot seedling, and it was 5% higher than its control (518kg/10a). Head rice ratios of pot seedlings and the controls were not significantly different in both transplanting dates.
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Kang, Shin-Gu,Hassan, Mian Sayeed,Ku, Bon-Il,Sang, Wan-Gyu,Choi, Min-Kyu,Kim, Young-Doo,Park, Hong-Kyu,Chowdhury, M. Khalequzzaman A.,Kim, Bo-Kyeong,Lee, Jeom-Ho The Korean Society of Crop Science 2013 한국작물학회지 Vol.58 No.3
A field study was conducted to understand nitrogen use efficiency of high yielding Japonica rice varieties under three levels of nitrogen fertilizer (90, 150 and 210 kg N $ha^{-1}$) in Iksan, Korea. Two high yielding rice varieties, Boramchan and Deuraechan, and an control variety, Dongjin2, were grown in fine silty paddy. Nitrogen use efficiencies (NUE) were 83.3, 56.3, and 41.2 in 90, 150, and 210 kg N $ha^{-1}$ fertilizer level, respectively. Total nitrogen uptake varied significantly among nitrogen levels and varieties. Variety Dongjin2 showed the highest nitrogen uptake efficiency (NUpE), while Boramchan and Deuraechan showed higher nitrogen utilization efficiency (NUtE). However, Nitrogen harvest index (NHI) was higher in Boramchan (0.58) than Deuraechan (0.57) and Dongjin2 (0.53). Rough rice yield showed linear relationship with total nitrogen uptake ($R^2$=0.72) within the range of nitrogen treatments. Boramchan produced significantly higher rough rice yield (8546 kg $ha^{-1}$) which mainly due to higher number of panicles per $m^2$ compared to Deuraechan (7714 kg $ha^{-1}$). Deuraechan showed higher number of spikelets per panicle, but showed lower yield due to lower number of panicle per $m^2$. Rice varieties showed different nitrogen uptake ability and NUE at different nitrogen level. Plant breeders and agronomist should take advantage of the significant variations and relationships among grain yield, NUpE, and NUE.
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Sang Gon Kim,Seonghyu Shin,Hwan Hee Bae,Jin-Seok Lee,Jung-Tae Kim,Min Jung Seo,Beom-Young Son,Jeom Ho Lee,Seong-Bum Baek 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Understanding the response of a crop to water deficiency is the first step towards breeding drought-tolerant varieties. In this study, inbred maize (Zea mays L.) lines KS140 and KS141 were subjected to drought stress by withholding water for 10 days at the V5 or V6 leaf stage. Water-deficient plants experienced a decrease in relative leaf water content, stomatal conductance, net CO2 assimilation rate, and water use efficiency compared to well-watered plants. This was accompanied by a decrease in the relative leaf water content that resulted in severe growth retardation in KS140 and KS141. However, leaf chlorophyll content in KS140 was unchanged. To understand the proteome dynamics during the 10-day drought stress in maize leaves, comparative proteome analysis was carried out between the well-watered and water-withheld leaves. Differential expression was observed for 29 protein spots from KS140 and 14 protein spots from KS141, and these were identified using MALDI-TOF mass spectrometry. Among identified proteins, metabolism and stress related proteins were highly were increased by drought stress. This study provides a protein profile of a Korean maize inbred line during drought stress, which will be valuable for future studies of the molecular mechanisms underlying drought resistance and for development of selective breeding markers for drought tolerance in maize.
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氣溫變異에 따른 多收性 品種 "多産벼" 의 生育特性 分析
김덕수,신진철,류점호 한국농림기상학회 2000 한국농림기상학회지 Vol.2 No.2
This experiment was carried out to find out the responses of the growth characteristics to various air temperature in a high yielding indica/japonica rice cultivar, "Dasanbyeo". Five different transplanting dates with 30 day-old seedlings were tried to induce various environmental conditions, starting from 10th May to 19th June in the interval of 10 days at National Crop Experiment Station, in 1998. Planting densities with different plants per hill but same hill spacing were tested. The daily mean air temperature during 30 days after transplanting was 19.0, 20.8, 21.9, 24.4 and 25.2℃ when transplanted on the 10th May, the 20th May, the 30th May, the 9th June and 19th June, respectively. The number of tillers per hill at 30 days after transplanting was affected by the daily mean air temperature during 30 days after transplanting. The higher the daily mean air temperature, the more tillers per hill at 30 days after transplanting were. The growth duration from transplanting to heading was greatly changed according to the transplanting dates. It was shortened as the transplanting date was delayed until transplanted on the 9th June, but it was prolonged when transplanted on the 19th June compared to that of the 9th June. The number of panicles per hill increased with the increase of the transplanted plants per hill up to 7 plants per hill. The number of panicle per unit area was closely related to the number of tillers per unit area at maximum tillering stage. It was found that the average effective temperature during first 10 tiller formation related to the days to the tenth tiller formation with the relationship of y=1.071x^2-37.307x+342.38(R^2=0.9823).
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