발표요지 : 포스터 발표 ; 참깨 ( Sesamum indicum L . ) 약배양에 의한 반수체 육성 Ⅰ. 생장조절물질이 Callus 유기에 미치는 영향과 Genotype 간의 차이

류점호,두홍수,권태호 한국식물생명공학회 ( 구 한국식물조직배양학회 ) 1992 한국식물생명공학회 춘계학술연구발표회 Vol.1992 No.-

종자의 성숙도가 Burley종 담배의 발아에 미치는 영향

柳點鎬,裵成國,韓喆洙,秋洪求 韓國作物學會 1983 한국작물학회지 Vol.28 No.2

본 시험은 발아가능한 담배종자를 조기 파종하여 파종에 이용하고자 몇가지 버어리종 담배를 공시하여 수분후 8일부터 30일까지 2일간격으로 채종 발아조사를 하였던 바 1. 종자는 수분후 12일 이상이면 발아가능하여 파종에 이용할 수 있었고 발아율은 낮았으며 품종에 따라 변이가 컸다. 2. 적기채종시기는 수분후 24-28일이였다. 3. 과숙종자는 후숙이나 일시휴면현상을 보여 발아율이 감소하였다. This experiment was conducted to evaluate the effect of early seed harvest on germination of tobacco(Nicotiana tabacum L.) seeds. Seeds of seven burley tobacco were harvested every two days from 8 to 30 days after pollination and tested for germination. The results are; 1. Seeds harvested 12days after pollination germinated but germination rates were low and varied across cultivars. These seeds will provide viable seeds adequate for breeding program. 2. Germination rates of seeds harvested 24-28 days after pollination were high and showed none significant differences among cultivars. 4. Over-ripen seeds showed low germination rates cused by after-harvest or temporary dormancy.

버어리종 논담배용 품종 선발시험

류점호,조천준,한철수,류익상 한국연초학회 1984 한국연초학회지 Vol.6 No.2

This experiment was carried out to select the suitable burley varieties for paddy field. Two varieties, two $F_1$ hybrids and three $F_1$ hybrids with pale yellow line which were all early maturing were tested in the paddy field condition. The results were as follows. 1. Va 528 and all $F_1$ hybrids flowered 4-8 days earlier than Burley 21. 2. Leaf maturity among varieties and $F_1$ hybrids were not significantly different at first priming, but significant differences were detected from second to forth primings. (MS Ky 12x JB 7802-30)Fl and (MS Br 21 x L8)$F_1$ hybrids matured 3-5 days earlier than Burley 21. Pale yellow hyorids matured early, yielded high and were favorable in physical properties, but were low in quality. 4. Va 528 and (MS Br 21 x L8) $F_1$ hybrid showed earthy maturing, high yield and good quality compared with Burley 21. So two varieties were considered suitable for paddy field cultivation.

Asparagine 이 수도 유묘기 내염성 향상에 미치는 영향

류점호,김경아,권태호 한국육종학회 1996 한국육종학회지 Vol.28 No.2

알팔파의 Glyphosate 저항성 세포주 선발

류점호 한국식물생명공학회 1997 식물생명공학회지 Vol.24 No.6

알팔파의 자엽과 하배축을 배양하여 캘러스를 유기하고 이들을 glyphosate첨가 액체배지에서 배양하여 glyphosate에 대한 생장억제 반응을 검사하였으며 10 mM glyphosate에 저항성인 세포주를 선발하고 EPSPS 활성을 조사 하였던 바 캘러스 유기율은 2,4-D 1 mg/L, kinetin 0.5 mg/L에서 가장 높았고 자엽보다 하배축이 캘러스 유기율이 높았으며, glyphosate 농도에 대한 ID$_{50}$은 0.1 mM과 0.2 mM사이에 있었다. A49-10G와 A58-10G는 대조세포주에 비해 각각 8.0배 와 9.1배의 높은 EPSPS 활성을 보였다. Calli were induced from the cotyledon and the hypocotyl of alfalfa and the callus lines were tested for the resistance to glyphosate in the liquid medium containing 0.01-3.00 mM glyphosate. Some resistant cell lines were selected from the gradual increase of glyphosate concentration and the lines resistant to 10 mM glyphosate were analyzed with EPSPS activity. Vigorous callus proliferation from the cotyledon and the hypocotyl was observed from the MS medium containing 1 mg/L 2,4-D and 0.5 mg/L kinetin. The hypocotyl was thought to be better explant source for callus induction than the cotyledon. $ID_{50}$ (Inhibition Dosage of 50%) to glyphosate was between 0.1 mM and 0.2 mM level. A49-10G and A58-10G cell lines selected as resistant to 10 mM glyphosate had 8.0 and 9.1 fold increased EPSPS activity to those of the control lines, respectively.

파종 및 정식시기가 사철쑥의 생육에 미치는 영향

류점호 ( Jeom Ho Ryu ),곽동욱 ( Dong Ok Kwak ) 전북대학교 농업과학기술연구소 2005 농업생명과학연구 Vol.36 No.-

홍화의 발아와 유모 생육에 미치는 몇 가지 스트레스의 영향

류점호 ( Jeom Ho Ryu ),서정원 ( Jeong Weon Seo ),강천식 ( Chon Sik Kang ) 전북대학교 농업과학기술연구소 2005 농업생명과학연구 Vol.36 No.-

삼요소 시비에 의한 야콘의 생육 및 수량반응

두홍수,류점호,추병길 전북대학교 농업과학기술연구소 2002 農大論文集 Vol.33 No.1

Nitrogen, phosphate and potassium were fertilized at 4 levels, respectively, to find the effects on growth and yield in yacon(Polymnia sonchifolia Poepping & Endlicher). Plant grew effectively as the amount of nitrogen was more and more, branch number was not difference, but plants lodged seriously in 24 kg/10a application. Plant height was not difference with 115~117 cm tall even though the amounts of phosphate and potassium were increased, so nitrogen effected to growth of shoots. Phosphate application was little effected growth and yield and was showed the irregular tendency. Potassium application was not effected to growth of shoots but the fresh weight of root and crown bud per unit area increased by potash fertilizing. Therefore, optimum fertilizing was 6-7-30(N-P_2O_5-K_2O) kg/10a for the best growth of shoot and root and plants were not lodged. The result of 3^3 factorial experiments, individual fertilizers, such as nitrogen and phosphate, effected and co-effected significantly to plant height, and interaction between nitrogen and phosphate co-effected significantly to tuberous root. Nitrogen, phosphate and potassium affected to main effects and phosphate and potassium influenced interaction effects for yacon growth below fround. In yacon cultivation, therefore, nitrogen and phosphate may be fertilized with 10 kg/10a or less than and potassium may be fertilized with 20 kg /10a,. these were the most effective on growth and yield. Key words : Polymnia sonchifolia, nitrogen, phosphate, potassium, amount, 3^3factor

Response of Different Seedings to Growth and Yield in Yacon

HongSooDoo,류점호,최선영,이강수,박기훈,YoungKeunCheong 한국작물학회 2002 Korean journal of crop science Vol.47 No.6

The seedlings of yacon (Polymnia sonchifolia Poeppig & Endlicher), which were cuttings, plug seedling (PS), crown bud before sprouting (CBBS), crown bud after sprouting (CBAS), and divided seedling after budding (DSAB) were planted at 70×50 ㎝ planting distance on ridge; that was interow spacingintrarow spacing, and about 28,500±71 plants/ha. CBBS didn''''t need work and equipment to raise seedlings. PS and DSAB grew taller to 140.5 and 143.3㎝, respectively, than others at 150 days after planting. In the changes of plant height, PS and DSAB showed taller than others during growth period, cuttings, CBBS, and CBAS grew rapidly in middle growth stage. Excepting main stem and petiole length, other characters were significant for seedling. Fresh weights were different among seedlings. Even though the yield of plants grown from CBAS and CBBS were lower with 34.7 and 36.4 ton/ha, respectively, than 3.6 ton/ha of DSAB; its yield index were over 95%, hence, those of plants grown from cutting and PS were lower with 73 and 87%, respectively. The ratio of tuberous roots over 200g to total tuberous roots per plant was the highest from DSAB. Most of tuberous roots were under 200g per tuberous root from cuttings. CBBS, CBAS, and DSAB are suitable to use seedlings for high yield of yacono. Yacon plant by DSAB much produced tuberous root of over 200g.

백두산지역과 국내 더덕 수집종의 RAPD에 의한 유연관계 분석

두홍수,류점호,이강수,이호림,유헌호 한국약용작물학회 2002 한국약용작물학회지 Vol.10 No.3

한국과 중국 동북부 지역에서 수집한 16종의 더덕으로부터 genomic DNA를 추출한 후, Bioneer사로부터 구입한 random primer(10-mer)를 이용하여 PCR을 수행하였다. 총 49종의 primer를 스크린 한 결과 재현성이 있으면서 polymorphism을 보이는 20개의 primer를 선발하였다. 선발한 primer로부터 PCR에 의해 증폭된 DNA의 크기는 125 bp에서 2.0 kb 내외였으며, 총 148개의 band가 관찰되어 평균 band 수는 7.4개였다. 이들 중에서 polymorphism을 보이는 band의 수는 73개이었으며 (49.3%), polymorphism은 각 primer별로 1~9개로써 다양하였다. 16개 수집종의 유사계수 범위는 0.682에서 0.959로써 유전적 유연정도는 크지 않았다. UPGMA 분석에 의한 수집종들의 유연관계를 dendrogram으로 나타낸 바, 수집종들간의 유전적 거리는 0.133~0.400이었으며, 국내 수집종과 중국 수집종간에는 확실하게 두 그룹으로 분류되었고, 유전적 거리는 약 0.281이었으며, 이들은 모두 지역적인 차이를 보였다. 한편, 중국의 '통화현(通化縣)'과 '류하현(柳河縣)' 수집종이 다른 지역의 수집종보다 유전적 거리가 가장 크게 나타났다. Extracted genomic DNA from 16 accessions of Codonopsis lanceolata collected from South Korea and the Baekdoo Mt. areas of China were analyzed for their genetic relationships by RAPD. Twenty 10-mer-oligonucleotide primers having reproductive polymorphism were selected for the RAPD analysis. The size of amplified DNA was almost between 125 bp and 2.0 kbp. Sixteen collected Codonopsis lanceolata were analyzed with 20 primers which generated 73(49.3%) polymorphic bands among 148 PCR products. The mean number of polymorphic bands were 7.4 and varied 1~9 per primer. It was, thus, demonstrated that RAPD was useful for detecting polymorphism in Codonopsis lanceolata. The range of 1-F value(genetic similarity) was from 0.682 to 0.959. These results indicate variable genetic similarities. By UPGMA (Unweighted Pair Group Method using an Arithmetic average) cluster analysis based on 1-F value, genetic distance among the 16 collected Codonopsis lanceolata was 0.133~0.400. It was certainly classified into two groups between collected accessions from Korea and China, and the genetic distance was about 0.281. Both accessions collected from Korea and China showed miner differences, while the genetic relationships of Tonghua Xian and Liuhe Xian from China was farthest with other accessions collected.