동일한 시료에 대한 국내 기관간의 STR 분석결과 비교 : STR 유전자좌 분석법의 표준화 설정을 위하여

박종태,신경진,양윤석,우광만,이숭덕,이승환,이정빈,정연보,조승희,한길로,한면수,홍승범 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

This paper described a collaborative exercise intended to see what kinds of short tandem repeat (STR) loci are used in different DNA typing laboratories in Korea and to compare their results for the demonstration whether uniformity of DNA profiling results from different laboratory could be achieved in Korea Laboratories were asked to test five tissue DNAs using methods routinely used in each laboratory and to report the results to the coordinating laboratory. The exercise demonstrated that each laboratory was using different STR loci for the typing with different STR numbers,2 VNTRS,36 STRs and amelogenin in total, and the direct comparison of the results from all the laboratory for the 18 loci could not be done as only one laboratory submitted typing results. Among 21 loci for which several laboratories submitted typing results, results for 14 loci were the same and results for the other 7 loci were different depending on the participating laboratory. D1S80, F13A01, D16S539, D21S11, D18S51, D3S1744 were the loci with different typing results. Even in the cases where commercial kits were used, the results were not the same depending on the machines used, that is the capillary electrophoresis or the gel based electrophoresis. The reason for the different results, points about the standardization of the methods arid the profiling data were described.

STR 유전자의 개인식별력 측정을 위한 통계량

이혜승,이재원,한길로,황적준 大韓法醫學會 1998 대한법의학회지 Vol.22 No.2

When we intend to do the personal identification using DNA profiles, it will obviously be better to use as much information as possible. The power of identification is increased by using the genetic marker system such as VNTRs or STRs. Although STRs do not have many alleles per locus as VNTRs, these can be compensated by a large number of loci that are potentially usable. However, it will be more efficient to use a morphic loci. Therefore, prior to choose the genetic marker system of STRs for identification, it is essential to consider the statistical parameters of each STR locus, such as obs-H(observed heterozygosity), exp-H(expected heterozygosity), pM(probability of match), DI(discrimination index), PD(power of discrimination), MEC(mean exclusion chance), MEP(mean exclusion paternity), PIC(polymorphic information content) et al. In this article, we described the exact meaning of statistical parameters for the purpose of identification.

바나듐 산화막을 활용한 습도센서의 제조에 관한 연구

이승철,최복길,김남철 公州大學校 工學硏究院 生産技術硏究所 2004 論文集 Vol.12 No.-

Vanadium oxides have been widely used in a variety of technological applications such electrochromic devices as infrared detectors and are expected as a material suitable for gas sensing applications. Thin films of Vanadium oxide (VOx) have been deposited by r.f magnetron sputtering under different oxygen partial pressure ratios(0%, 10%) and substrate temperatures(27℃, 400℃). Humidity-sensitive properties of resistive sensors having interdigitated electrode structure are characterized. Our sensors show good response to humidity over 20%RH to 80%RH Vanadium oxide films deposited with 0%O2 partial pressure at 400℃ exhibit greater sensitivity to humidity change than others.

냉기치료후 슬관절강내의 온도변화

백승석,최기섭,박시복,이상건,김영호,양길태,장윤희 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.2

스퍼터된 바나듐 산화막의 구조적, 전기적 특성에 미치는 진공 어닐링의 효과

이승철,황인수,최복길 公州大學校 工學硏究院 生産技術硏究所 2003 論文集 Vol.11 No.-

Thin films of vanadium oxide(VO_(x)) have been deposited by RF magnetron sputtering from V₂O_(5) target in gas mixture of argon and oxygen. The oxygen/(oxygen+argon) partial pressure ratio of 0% and 8% is adopted. Crystal structure and optical properties of films sputter-deposited under different oxygen gas pressures and in situ annealed in vacuum at 400℃ for 1h and 4h are characterized through XRD, XPS, RBS, FTIR, and optical absorption measurements. The films as-deposited are amorphous, but 0%O₂ films annealed for time longer than 4h and 8%O₂ films annealed for time longer than 1h are polycrystalline. I-v characteristics were distinguished between linear and nonlinear region. In the low field region the conduction is due to Schottky emission, while at high fields it changes to Fowler-Nordheim tunneling type conduction. The conductivity measurements have shown an Arrhenius dependence of the conductivity on the temperature.

肥肉豚의 血液化學値에 關한 硏究

李政吉,康炳奎,魏聖河,朴承柱,朴來生 全南大學校 農漁村開發硏究所 1981 農業科學技術硏究 Vol.16 No.1

全羅南道 順天地方을 中心으로 한 養豚場에서 出荷된 肥肉豚을 對象으로 血液化學値를 調査했다. 돼지의 年齡은 7個月부터 13個月이었으며, 總 276頭(♀ 181,♂ 95)를 對象으로 屠畜場에서 採血했는데 Landrace와 Berkshire의 一代難種이 大部分이었다. 한편 本 實驗에서 使用한 무??豚은 모두가 去勢한 것이었다. 1. 測定한 血液化學値는 血淸?萄糖量, 血淸總蛋白量, ablumin量, globulin量, 血淸尿素?素量, 血淸 cholesterol量, calcium量, 無機燐量, 血淸 transaminase(SGOT와 SGPT)等이었다. 2. 本 調査에서 나타난 각종 血液化學値의 範圍 및 平均値는 이미 報告된 結果와 거의 一致하였다. 3. 血淸cholesterol量은 性別差가 없었고 겨울에 높은値를 보인 反面, 여름과 가을에는 낮은 치를 보였다. 4. 血淸總蛋白量의 季節的變動은 globulin量의 變動에 起因한 것이었고, globulin量은 全般的으로 높게 나타났는데 特히 여름철의 增加가 뚜렷했다. Samples of blood were taken from 276 fattening pigs(181 intact females and 95 castrated males) at slaughter and serum chemical values were determined. The pigs were from Suncheone area, Chonnam Province; their age ranged 7 to 13 months; most of them were crossbreds of Landrace and Berkshire. 1. Determined blood chemical values in the prestent study were glucose, total protein, albumin, globulin, serum urea nitrogen, cholesterol, calcuim, phosphorus, serum glutamin oxalacetic transaminase and serum glutamic pyruvic transaminase. 2. The ranges and means of the chemical values in the present study were reconcilable with the normal values previously reported. 3. Sex differences between castrated males and intact prepubertal females were not observed in serum cholesteol levels. There were significant differences between the means of seasons, with the higher cholesterol values being observed during the winter as compared with summer and fall. 4. Seasonal changes of total protein cantent were due to the seasonal changes of globulin values. The globulin values in the present study were higher than those reported previously by others; the values were highest during summer season.

경찰공무원의 임파워먼트가 직무만족에 미치는 영향에 관한 연구

이승길 한국경찰학회 2003 한국경찰학회보 Vol.5 No.2

임파워먼트는 직무에 대한 불만, 사기저하, 근무의 비능률성, 잘못된 직무수행, 위험스런 업무의 회피 등의 해결방안으로 표현되고 있고, 대부분의 관리자들이 임파워먼트의 장점을 이해하고 도입을 환영하고 있다. 임파워먼트를 실시하는 것은 권한이 하부로 상당히 이양되는 것으로 경찰관리자가 부하직원이 잠재력을 개발하도록 인식하고 실행하는 것으로 경찰조직에서 반드시 필요하다. 따라서 본 논문에서는 경찰공무원의 임파워먼트가 직무만족에 어떠한 영향을 미치는지 실질적으로 파악하는데 연구의 목적이 있으며, 그 결과로서 경찰공무원의 임파워먼트가 높을수록 구성원들의 직무만족도는 높은 것으로 밝혀졌다. In this situations, the employees autonomy and creativity within the organization are as important as the real change at the individual level. One of the most important management techniques is considered to be the empowerment for this. The objectives of this study are to examine the theoretical frame of the concept of psychological empowerment(meaning, competence, self-determination, impact), and how empowerment of individual level of police officers influence on job satisfaction. The methodology used in this study is based largely on the theory of G. M. Spreitzer. A questionaire comprises two catagories; empowerment and job satisfaction. Empowerment related questionaire comprised four variables; meaning, competence, self-determination, impact). And, job satisfaction questionaire includes some statements on effect factors which represent the relationship between the job attitudes and the resulting behaviors.

한국인에서 다변성 D12S391 유전좌의 집단유전학적 연구

이용욱,김성민,구태완,이혜린,강일호,한길로,이혜승,황적준 大韓法醫學會 1999 대한법의학회지 Vol.23 No.1

The hypervariable short tandem repeat(STR) locus D12S391 was investigated in a Korean population. A total of 14 alleles were detected by size under denaturing conditions in 517 unrelated individuals. To confirm all of the alleles detected in a Korean population, a total of 34 fragments were sequenced. Prior to allele designation, we constructed the allelic ladders containing 11 alleles sequenced in this study. Allele 18 is the most common with a frequency of 0.281 in Koreans, and one variant allele 19.3 which have been confirmed by sequencing, was detected. The observed heterozygosity, the power of discrimination (PD), and the mean exclusion chance (MEC) for the locus D12S392 is 0.781, 0.946 and 0.652 , respectively. No deviation from Hardy-Weinberg equilibrium was observed in a Korean population (p=0.557). In the 424 meioses in 105 Korean families confirmed using other 17 STR loci, no mutation was detected in locus D12S391. The STR locus D12S391system is useful both for the analysis identification and parternity.

참외주스 가열농축에 따른 관능적 특성 변화

이기동,권승혁,이명희,김숙경,주길재,권중호 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2004 한국식품저장유통학회지 Vol.11 No.1

고품질 참외 농축액을 제조하기 위해 농축공정에서 중요한 변수로 작용하는 가열시간을 달리하면서 제조한 농축물에 대한 관능적 평가를 실시한 결과, 가열 후 맛이 최대평점을 나타낸 조건은 가열온도가 96.63 ℃ 및 가열시간이 14.31 min일 때 였으며, 전반적인 기호도가 가장 높은 조건은 가열온도가 97.18℃ 및 가열시간이 14.55 min 일 때 였다. 가열온도 및 가열시간을 달리하면서 실험한 결과 참외 농축액 제조를 위해서는 가열온도 98℃로, 가열시간 13 min으로 처리하는 것이 적당하였다. This study was carried out to observe the change of organoleptic properties of the juice prepared with various heating temperature and heating time. Organoleptic color showed maximum value at 94.95℃ and 21.63 min and organoleptic aroma showed maximum value at 63.14℃ and 20.38 min. Organoleptic taste showed maximum value at 96.63℃ and 14.31 min and overall palatability showed maximum value at 97.18℃ and 14.55 min The best condition was 98℃(heating temperature) and 13 min(heating time) at oragnoleptic evaluation of oriental melon juice.

DNA 증거의 집단유전학적 분석을 위한 SAS시스템

이효정,이혜승,한길로,이재원,황적준 大韓法醫學會 2000 대한법의학회지 Vol.24 No.1

DNA analysis has become one of the most powerful tools in forensic inference for human identification and is now used worldwide. It is used to be statistical technique for the individual identification of a civil and criminal action. The purpose of this article is computerization of the statistical technique for the population study and DNA evidence analysis. The system using SAS/AF and SAS/SCL is the graphic user interface and the correspondence of the changed experimental circumstances