폐결핵 환자 혈청에서 수용성 Interleukin 2 수용체 및 Adenosine Deaminase 활성도에 관한 연구

고정희,박성규,백상현,박찬권,박병수,안진영,최우석,박정규 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

Interleukin-2 (IL-2) induces T cell proliferation in an autocrine manner and provides a means by which antigen triggered T cells can be clonally expanded in vitro. During the following activation, the activity of IL-2 is mediated by specific high affinity IL-2 binding membrane receptors which are expressed shortly after activation. In this process, a 42 KD-fragment (soluble IL-2R) is continuously cleaved off and circulates as a soluble marker of T lymphocyte activation. Elevated level of soluble IL-2R has been identified in the serum of patients with malignant autoimmune and allergic disorders, systemic parasitic infection, undergoing graft versus host disease, acute or chronic lymphocytic leukemia and HIV-infection. ADA (adenosine deaminase) completes the process of differentiation of T cell and is essential for progression of T cell maturation. Therefore level of ADA is to correlated with magnitude of T cell immune response. The fact that expression of sIL-2R and ADA activity increases in peripheral blood T-lymphocytes of patients with active pulmonary tuberculosis suggests that T cell activation might have a major role in the pathogenesis of pulmonary tuberculosis. In order to evaluate the T cell immune response in pulmonary tuberculosis, we measured the serum concentration of sIL-2R and ADA activity in 17 patients with current pulmonary tuberculosis, 10 chronic inactive pulmonary tuberculosis, 10 as normal controls. (1) Current pulmonary tuberculosis had significantly higher levels of sIL-2R (237.24±95.47)when compared with those of inactive tuberculosis (78.6±11.06). and the control (68.17±15.4) group. (2) ADA activity in current pulmonary tuberculosis was significantly increased (34.41±20.63) when compared with those of inactive tuberculosis (24.7±14.36) and control (17.65± 5.94) group. (3) There was good correlation between sIL-2R concentration and ADA activity in serum in current pulmonary tuberculosis group. (4) sIL-2R concentration and ADA activity was decreased significantly 6 months after anti-tuberculosis drug medication. In conclusion, sIL-2R concentration and ADA activity in serum in current pulmonary tuberculosis group was increased when compared with those of inactive pulmonary tuberculosis and the control group. By the way, T cell mediated immune response was enhanced in current pulmonary tuberculosis, but in inactive chronic pulmonary tuberculosis who had treated by antituberculosis drug medication, the concentration of sIL-2R and ADA activity was nearly normal.

마우스 골수세포 배양시 Prostaglandin E_2가 파골세포-유사세포 생성에 미치는 영향에 관한 연구

고성희,김관식,정동균 대한구강생물학회 1990 International Journal of Oral Biology Vol.14 No.2

Osteoclasts are primary cells responsible for bone resorption. It has been reported that osteoclasts are formed by fusion of mononuclear precursors derived from hematopoietic progenitor cells. Prostaglandin E_2(PGE_2) is known to be a potent bone resorbing agent, but its mechanisms are not known. This experiment was performed to study the effect of PGE_2 and calcitonin on the generation of osteoclasts from their precursor cells. The bone marrow cells were prepared from 7 to 9 week-old male mice. The femur and tibia were dissected aseptically and the marrow cavity was flushed with 1ml α-minimum essential medium by slow injection. The collected marrow cells were cultured at 1.5∼2.0×10^6 cells/well in 24-well plate for 5, 8 and 11 days. In experimental group, PGE_2(10^-5,10^-6,10^-7,10^-8M) and calcitonin(5, 30, 90ng/ml) were added. After cultures, staining for tartrate-resistant acid phosphatase(TRACP)-marker enzyme of osteoclast-was performed according to the modified method of Burstone. The TRACP-positive multinucleated cells, which have 3 or more nuclei were counted, The observed results were as follows. 1. In control group, TRACP-positive mononuclear cells were present, but no TRACP-positive multinucleated cells appeared. 2. In 8 days of culture, PGE_2 increased significantly the number of osteoclast-like cells in a dose-dependent manner in the mouse marrow cell cultures. 3. In 5, 8 and 11 days of culture with PGE_2(10^-6M), the number of osteoclast-like cells was increased up to 8 day and decreased thereafter. 4. In 8 days of culture, calcitonin decreased significantly the number of osteoclast-like cells induced by PGE_2(10^-6M) in a dose-dependent manner.

악관절 장애와 두개안면 동통의 해소를 위한 약리학적 접근

고성희 대한 두개하악 장애학회 1999 대한두개하악장애학회지 Vol.11 No.2

The management of temporomandibular disorder (TMD) and craniofacial pain may involved the use of a number of therapeutic approaches, including pharmacotherapy. Pharmacological intervention in the management of TMD and craniofacial pain usually is considered an adjunctive therapy because more definitive treatments typically are used to correct the underlying pathophysiological process. However, pharmacotherapy often is the primary approach to treating depression and the inflammatory processes that are frequently associated with TMD. This article provides overview of the pharmacology of the drugs that have been used in management of TMD and craniofacial pain. The brief discussion about mode of action, pharmacologic effects, adverse effects and drug interactions of nonsteroidal anti-inflammatory drugs, opioid analgesics, antide-pressants, muscle relaxants, benzodiazepines, steroids, and local anesthetics is included, and guidelines for their use are suggested.

청국장의 제조방법에 따른 향미 증진 효과

고한수,조대희,황성연,김영만 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.1

청국장 제조를 위한 분리균은 포자형성율이 높은 Bacillus subtilis로 대사력이 활발한 대수증식기의 것을 콩에 1,000CFU/g 이상의 균수를 접종하여 40℃와 상대습도 90%에서 배양하면 발효가 빠르게 진행되었다. 발효가 종료되면 포자형성율이 95% 이상 되어야 저온의 후숙과정에서 재번식이 쉽게 일어나지 않았으며 발효취는 발효 중에서만 형성되었다. 중립콩과 대립콩의 발효율이 가장 좋았다. 저온의 후숙과정은 5℃에서 1∼2일 후 경과하면 Bacillus subtilis는 더 이상의 증식이 일어나지 않고 발효취가 소멸되므로 저온의 유통과정을 거쳐야 불쾌취가 없는 구수한 청국장의 맛을 유지할 수 있었다. The strain isolated for making chungkuk-jang was Bacillus subtilis, which formed spore with 98% ratio. Logarithmical culture was inoculated (1,000 CFU /g) to the steamed soybeans and at the optimum fermentation conditions(40℃, RH 90%), fermentation progressed very rapidly and synchronously. Fermentation time was 24 hours on the optimum fermentation conditions. During activated fermentation, chungkuk-jang's aroma and flavor created. After finishing the fermentation, the spore forming ratio was 95% and replenishment was not occured easily during aging at the below 5℃.

장애인 생활시설의 후원자원 개발을 위한 전략적 마케팅 기획 사례연구 : J기관을 중심으로 Focused on J Institution

고선정,김성희 한국장애인재활협회 2004 재활복지 Vol.8 No.1

본 연구는 장애인생활시설의 후원자 개발을 위한 전략적 마케팅 기획과정(Strategic Marketing Planning Process)에 따른 사례연구를 하고자 함이다. 최근 사회복지시설은 후원자원 개발의 필요성이 제기되고 있으나 담당인력의 마케팅 기법에 관한 지식과 기술이 미숙한 단계에 있다. 특히 작은 규모의 생활시설은 자원개발의 필요성에도 불구하고, 후원자원 개발을 위한 마케팅 전략을 수립하는데 어려움을 겪고 있다. 이를 위해 장애인생활시설(J기관)의 후원자개발 사례를 종합하여, 전략적 마케팅 기획과정에 초점을 맞추어 조직을 분석하고, 그 내용을 토대로 포트폴리오 기획을 통해 핵심 마케팅 전략을 수립하였다. 특히 조직적 차원에서 마케팅 전략을 수립하기 위해 Andreasen과 Kotler(2003)가 제시하고 있는 기본들을 사용하였다. J기관의 조직분석 결과, 도서지역이라는 지리적 특수성은 자원개발을 하는데 저해요인이 되고 있었으나, 기관의 약점을 강점으로 활용하기 위한 기회요인(시설의 개방)을 찾고, 후원자개발을 위한 조직문화를 형성하고 있음을 볼 수 있었다. 특히 마케팅믹스에 의한 자원개발 전략을 7가지 요소로 구분하여 최적의 상태에서 자원개발을 하기 위한 방안을 모색하였다. 따라서 본 연구는 생활시설의 담당인력이 자원개발 업무수행에 어려움을 겪고 있는점을 감안할 때, 단일기관의 사례를 제시함으로써 생활시설의 자원개발에 따른 실천적 접근방법을 모색하는데 그 의미가 있겠다. This is a case study on the process of strategic marketing planning to develop sponsors of residential institutions for people with disability. Recently social welfare institutions are raising the necessity of developing sponsoring resources but persons in charge of relevant affairs lack knowledge and skills in marketing. In particular, small-sized residential institutions have difficulties in establishing marketing strategic for the development of sponsoring resources despite their keen necessity of resource development. Thus this study examined a case of sponsor development by an residential institution for people with disability(J Institution), analyzing the organization focused on the process of strategic marketing planning. In addition, based on the findings, it established a key marketing strategy through portfolio planning. Particularly to establish a marketing strategy in an organizational dimension, this study used a basic frame proposed by Andreasen & Kotler(2003). According to the result of analyzing J Institution, its geographical characteristic, namely, its being at an island was a factor hindering resource development but the institution had developed organizational culture for finding opportunities (openness in residential institution) to utilize its weakness as a strength and developing sponsors. In particular, it divided its resource development strategy based on marketing mix into seven elements, and looked for resource development plans under the optimal condition. Thus, considering difficulties that persons in charge of resource development at residential institutions for the disabled have in performing their job, this study is meaningful in that it looked for practical approaches for the institutions to develop resources by suggesting the case of an institution.

기종성 방광염 1예

마성권,나명윤,박종욱,고정희,염충호,김수완,김남호,최기철 全南大醫大 2001 전남의대학술지 Vol.37 No.2

Rhizobium 렉틴의 분리ㆍ정제 및 특성 연구

전경희,정시련,이인경,고성진 嶺南大學校 基礎科學硏究所 1993 基礎科學硏究 Vol.13 No.-

Table 1. Specificity of Rhizobium sp. crude lectin in agglutinating erythrocytes from various animals (그림) [HU]: hemagglutinatin unit Table 2. Specificity of intracellular crude lectin from Rhizobium sp. in agglutinating of erythrocytes from various animals (그림) [HU]: hemagglutinatin unit Table 3. Hemagglutination of intracellular crude lectin from Rhizobium sp. in culture age (그림) ※[HU]: hemagglutinatin unit (그래프) Fig.6 Stability of Rhizobium japonicum iectin activity at different pH. Rhizobium japonicum crude lectin was incubated in buffers at different pH(2.18-10.80) for 24hr at 4℃ and the remaining activity was determined. Table 4. Effect of mental ions and chelating agent in hemagglutinating activity for Rhizobium sp. crude lectin (그림) *Chelating agent (그래프) Flg.7 Stability of Rhizobium japonicum iectin activity at different temperature. Rhizobium japonicum was incubated for 30min. After immediate cooling in an ice-water bath, the remaining activity was determined. 조사한 결과 3일째 부터 약한 활성을 나타냈으며, 6일째에 32HU로 최대의 활성을 나타내었다. 배지로 분비된 렉틴은 사람 적혈구와 토끼 적혈구에서 응집현상을 나타내었으며 trypsin처리후 토끼와 DEAE 0.2M 분획에서 하나의 major band와 minor band가 나타났으며, Rhizobium sp. 렉틴의 활성은 pH 7-9범위에서 안정한 것으로 나타났으며, 또한 온도의 영향은 50℃이하에서 안정하였다. 적혈구 응집 저해 효과는 25mM galactose에서 최대로 나타났다. 세포내 결합형 렉틴을 배양시기(1, 2, 4, 6일)에 따라 전기 영동 시험을 실시한 결과 동일한 band pattern과 렉틴 활성을 나타냈으므로, 세포내 결합형 렉틴은 배양 시기 별로 차이가 없음을 알 수 있었다. Table 5. The comparison of protein and carbohydrate contents in Rhizobium sp. crude lectin (그림) a : YMA broth media b : Cell was ruptured by sonication. Crude lectins were partially purified from Rhizobium sp. by salt fractionation and ion exchange chromatography on DEAE-Sephadex A-50. Hemagglutination of extracts from culture of Rhizobium sp. with age showed remarkable activity in 6 days. With purified lectin several biochemical properties have been characterized: Rhizobium sp. lectin agglutinated nonspecifically erythrocytes of human and several animals. In SDS-polyacrylamide gel electrophoresis, Rhizobium sp. lectin (0.2M fraction of DEAE Sephadex A-50) showed one major and one minor bands. Crude lectin from Rhizobium sp. was relatively stable at the range fo pH 7-9 and temperature below 50℃. In sugar inhibition test, this lectin was inhibited by only galactose at final concentration of 25mM. Meanwhile, intracellular crude lectin from Rhizobium sp. with culture age showed same band patterns by polyacrylamide gel electrophoresis.

첨가성분에 따른 부가중합 실리콘 인상재의 세포독성에 관한 연구

조리라,고성희,정경호,김경남 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.3

The purpose of this study was to evaluate the biocompatibility of polyvinyl siloxane impression materials and it's components using cell culture techniques. In the cytotoxicity test, using the culture of L929 mouse fibroblast, the degree of gross cytotoxic effects on the cells by changes in the cell morphology was scored for each materials. And the changes in the cell membrane permeability was tested using the agar overlay test. The results were as follows: 1.The effect of Examix impression material had on cell viability by evaluating survival rates was more severe than any other materials and newly added surfactants. 2.NP-4, nonylphenoxy polyethylene ethanol homologs, showed some cytotoxicity than PC-550 and BYK066. 3.In the test of the changes in cell membrane permeability using the agar overlay method, any cytotoxicity was recorded in silicone base groups. 3.In agar overlay test, Examix and NP-4 showed most response index and lysis index. 4.The application of surfactant such as PC-550, NP-4, and BYK066 might be considered relatively biocompatible.

cAMP 농도에 영향을 미치는 수종약물이 파골세포형성에 미치는 영향

소영,고성희,백정화,김관식,정동균 대한구강생물학회 1992 International Journal of Oral Biology Vol.16 No.2

To study the effect of cAMP on the generation of osteoclasts from their precursor cells, the bone marrow cells were isolated from 7 to 9 week-old male mice. The femur and tibia were dissected aseptically and the marrow cavity was flushed with 1 ㎖ of α-minimum essential medium by slow injection. Collected marrow cells were cultured at 1.5-2.0×10^6 cells/well in 24-well plate for 8 days. In experimental group, PGE_2(5×10^-6, 10^-5M), forskolin(10^-5M) or IBMX(10^-4, 10^-5M) were added singly or in combination from the 1st day culture. After cultures, staining for tartrate-resistant acid phosphatase(TRACP)-marker enzyme of osteoclast-was performed according to the modified method of Burstone. The TRACP-positive multinucleated cells(MNC), which have 3 or more nuclei, were counted. The observed results were as follows. 1. In control group. TRACP-positive mononuclear cells were present, but no TRACP-positive multinucleated cells appeared. 2. PGE_2(5×10^-6M) or forskolin(10^-5M) significantly stimulated the formation of TRACP-positive MNC. Moreover, forskolin potentiated the TRACP-positive MNC formation induced by PGE_2 when added simultaneously. 3. IBMX(10^-4, 10^-5M), when added alone, significantly stimulated the formation of TRACP-positive MNC. However, IBMX(10^-5M) added in combination of PGE_2(10^-5M) partially inhibited the TRACP-positive MNC formation induced by PGE_2.

Platelet-Derived Growth Factor가 백서두개관 세포군의 증식 및 교원합성에 미치는 영향

김기수,고성희,백정화,민병무,김관식,정동균 대한구강생물학회 1991 International Journal of Oral Biology Vol.15 No.2

To study the effect of platelet-derived growth factor(PDGF) on the replication and collagen synthesis of rat calvarial cells, five bone cell populations(I-V) were prepared from fetal rat calvaria by sequential enzyme digestion. After primary culture for 6-7 days, each bone cell population was collected and then population Ⅰ and Ⅱ, Ⅳ and Ⅴ were pooled together. And the cells were resuspended at 6-8×10^4 cells/㎝^2 and cultured for 2-3 days. The medium was changed to serum-free medium prior to addition of growth factor. The effect of PDGF on the cell proliferation was measured by the incorporation of [^3H]thymidine into DNA. Protein synthesis was determined by measurement of [^3H]proline incorporation into collagenase-digestible protein(CDP) and noncollagenous protein(NCP) according to the method of Peterkofsky and Die-gelmann(1971). The observed results were as follows. 1. PDGF at 10 ng/㎖ significantly increased the [^3H]thymidine incorporation into DNA in all bone cell populations. 2. PDGF at 30 ng/㎖ significantly increased the synthesis of NCP in population Ⅰ, Ⅱ and Ⅳ, Ⅴ. 3. PDGF had no effect on the synthesis of CDP but percent collagen synthesis was decreased significantly in population Ⅳ, Ⅴ. Taken together, the increase of protein synthesis by PDGF in rat calvarial cells was due to the incraese of NCP synthesis.