Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

進饌儀軌를 통하여 본 1887년 朝鮮王朝 宮中 進饌宴 중 萬慶殿 翌日灰爵과 萬慶殿 翌日夜연의 상차림에 대한 考察

金尙寶,李盛雨,朴惠苑,韓福眞,黃蕙性,韓福麗 동아시아식생활학회 1991 동아시아식생활학회지 Vol.1 No.3

To examine the following day party and the following evening party in the Man Kyong Jeon, the authors studied "Jin Chan Eui Gue", which is a historical reoord published in 1887(King Go-Jong). The results obtained from the study were as follows, 1.There were fixed arranging tables(22) and taking-away tables in the dining table of the following day party. 2.There were fixed arranging tables(5) and taking-away tables in the dining table of the following evening party. 3.Food and flowers were distributed to the 390 attendants in the following day party. 4.There were no distribution of food and flowers to the attendants in the following evening party. 5.Arrangements of the followings day party and following evening party were the same as those of the main party. 6.The arranged seat of the King, Go-Jung was facing the south. 7.According to the status or class of the attendant, the pattern or size of table settings were differentiated in the kinds of food, heights of food, table flowers, chinaware etc. 8.1n the arrangements of tables for the King, the heights of food on the dishes were as following levels, 1 dish : 1 chok(1尺) 6 dishes : 8 chon(8寸) 4 dishes : 7 chon(7寸)

화천의 저서성 대형무척추동물의 군집구조에 관한 연구

류성만,정동기,이종욱,차진열 嶺南大學校 基礎科學硏究所 1997 基礎科學硏究 Vol.17 No.-

The present study was conducted to determine the community structure in Hoe Stream based on the benthic macroinvertebrates. The survey area included nine sites in Hoe stream and two sites in Nakdong river. The study was carried out from March of 1995 to February of 1996. The result are as follows; (1) Benthic macroinvertebrates are composed of a total 200 species including 115 genera, 68 families, 21 orders, 7 classes in five phyla. 2) Average number of individuals was 1353.9/0.5㎡ in the whole study area. Individual number was variable from 10.2/0.5㎡ at the site 10 to 186.3/0.5㎡ at the site 4. Taxa with highest individual species frequency were aquatic insects. 3) Average dominance(DI), species richness(RI), species diversity(H'), and evenness(EI) were 0.62, 1.70, 3.12 and 0.048, respectively, in the whole study area. The biotic indices in Hoe river were relative higher than that of Nakdong river. 4) Functional feeding groups were collector at site 1 to 6, collector and predator at site 7 to 9, filterer and predator at site 10 and 11.

사이클링 선수의 슬관절각에 따른 하지동작의 운동학적 분석

류창엽,최성진,박종진,강순용 한국운동역학회 2001 한국운동역학회지 Vol.11 No.3

This study set out to evaluate the appropriateness of the height of a cycle saddle which was usually set based on a cyclist's experience and simple judgment. For this purpose, there was aroused a need to study what a cyclist's leg movements were according to the knee joint angle, that is, to study how a cyclist's leg joint angles and angular velocity would change at each pedaling. Seven male college cyclists were selected, and their pedaling movements were divided into four phases. The images were taped at 60fields/sec, using two video cameras. The KWON 3D 2.1 program was adopted to calculate and synchronize the 3D coordinates values which were calculated with DLT(Direct Liner Transformation), in order to analyze kinematical factors. The results were as follows: 1) There was found no difference between foot joint angles according to changes of knee joint angles in each phase. But it became clear that Phase 1 and 2 where the foot angle got bigger were the area where the foot joint was expanded and that Phase 3 and 4 where the foot angle got smaller were the area where the foot joint was bent. Accordingly, the area which gives a great indirect impact to the changes of foot joint angles is considered to be the first part of Phase 3 at which point the cycle of pedaling starts. 2) In terms of the difference of foot joint angular velocity according to knee joint angles, there was found a relatively stable deceleration in the decelerating area(Phase 3 and 4) while you could detect faster changes in the acceleration area(Phase 1 and 2) than in the decelerating area. 3) The changes in the angle difference of knee joints according to each knee joint angle showed almost similar patterns for every knee joint. But the patterns had the graph model of the opposite shape to that of the angle difference of foot joints. 4) The research went on to see what kind of difference the knee joint angle made as knee joint angular velocity changed. The changes were unlike the foot joint angular velocity and thus showed relatively slow deceleration and acceleration. And the higher the saddle was set, the bigger the difference of the angular velocity was. 5) The difference of hip joint angles according to knee joint angles made a graph pattern which showed no big correlation with the height of a saddle. 6) In considering how hip joint angular velocity would change according to knee joint angles, the higher a saddle was set, the bigger angular velocity was found in Phase 1 and 2, the area of joint expansion and the smaller angular velocity in Phase 3 and 4, the area of joint bending. Comparisons and analysis were made based on the results above mentioned. The conclusion was that the lower a saddle which was one of the three experiment conditions was set, the smoother the pedaling turned and the bigger the angular velocity was resulted. In other words, when you take a long road cycling with your saddle fixed at a little lower height than the average one, you will consume less physical strength and expect better performance.

매체순환식 가스연소기 적용을 위한 Ni계 및 NiO계 산소공여입자의 반응특성

류호정,배달희,조성호,진경태 한국화학공학회 2004 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.42 No.1

매체순환식 가스연소기에 적용하기 위한 최적의 산소공여입자를 결정하기 위해 금속산화물(NiO)의 함량 변화에 따라 네 가지 (NiO 26, 44, 58, 78wt%)의 산소공여입자를 제조하여 열중량 분석기에서 환원반응특성을 측정 및 비교하였다. 제조된 입자들 중에서 NiO 약 60wt%인 입자가 산소전달능력 및 반응속도 면에서 다른 입자들에 비해 우수한 성능을 나타내었다. 산소공여입자의 제조과정에서 금속산화물 형태의 영향을 살펴보기 위해 금속산화물의 출발물질로 Ni과 NiO를 사용하여 NiO 함량이 동일한(약 60%) 두 종류의 입자를 제조하였으며 열중량 분석기와 고정층 반응기를 이용하여 반응속도, 재생성, 환원반응 유지시간 및 CO_(2) 선택도 등을 측정 및 고찰하였다. 금속산화물의 출발물질로 NiO를 사용한 입자가 Ni을 사용한 입자에 비해 반복 실험시에 정상반응조건에 빨리 도달하였고 반응속도와 CO_(2) 선택도가 높았으며 환원반응 유지시간인 길게 나타났다. 실험결과에 의해 본 연구에서 고려한 산소공여입자 중에서 금속산화물의 출발물질로 NiO를 사용한 NiO 함량이 60wt%인 입자를 최적의 산소공여입자로 선정하였다. To find a suitable oxygen carrier particle for chemical-looping combustor, four kinds of particles (NiO 26, 44, 58, 78wt%) were prepared and the reactivity of the reduction was measured and investigated by a thermo-gravimetrical analyzer. From the viewpoint of oxygen transfer capacity and reaction rate, the particle containing 60wt% of NiO showed better performance than the other particles. Also, we have prepared two kinds of oxygen carrier particles to investigate the effect of the starting material for a metal oxide component(i.e., Ni based and NiO based particles, where the weight percents of both samples were set at 60wt% as NiO). The reaction rate, regenerative ability, duration of the reduction, and CO₂ selectivity were easured and investigated by a thermo-gravimetrical analyzer and a fixed bed reactor. The results obtained here indicated that the NiO based oxygen carrier particle containing 60wt% of NiO showed better regenerative ability, reaction rate, CO₂ selectivity, and duration of the reduction, compared with the other particles, therefore we selected this particle as an optimum oxygen carrier particle.

관절성형술 및 측두근막 이식술을 이용한 악관절 강직증의 치험례

진택현,오승환,류동목,김여갑,이상철 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.1

This is a case of bony ankylosis of right temporomandibular joint with mild facial asymmetry resulted from trauma, which bring about the right condylar fracture, and corrected by interpositional arthroplasty with temporal fascia flap and coronoidectomy. The postoperative results were functionally and esthetically good, but continued osbervation is necessary to evaluation of abnormal bony growth and reankylosis.

고정화 시스템을 이용한 용균효소의 생산

류병호,박종옥,진성현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

해양에서 분리된 용균활성이 우수한 균주의 균체고정화에 의한 연속생산을 위한 조건을 검토하였다. Bacillus subtilis SH-1의 균체를 고정화하여 column형 reactor에 의하여 연속적으로 효소생산을 실험하였다. 연속발효의 최적조건은 담체는 3.0% sodium alginate로 직경 2.4mm의 bead를 제조하여 기질의 주입속도는 20ml/h 공기의 주입속도는 2.0ml/min이였다. Column형 reactor(용량 2.8ℓ)에 의한 연속발효시 25일까지 76.5~88.0 units/ml의 최적의 효소활성을 갖는 용균효소를 생산할 수 있었다. Bacillus subtilis SH-1 screened from coastal sea water of South Korea was used to produce bacteriolytic enzyme. The production of bacteriolytic enzyme by immobilized cells was investigated. The optimum conditions for the continuous production of the bacteriolytic enzyme using immobilized cells were 2.4 mm diameter of 0.3% alginate beads, 20 ml/h of substrate feeding rate and 20 ℓ/min of aeration rate. A productivity of 76.5 to 88.0 units/ml could be obtained for 25 days by continuous column reactor under the optimum conditions.

담도 및 췌장 악성종양에서의 CAl9-9의 진단적 가치에 대한 평가

명승재,최승목,김은옥,최호순,이성구,김명환,민영일,류진숙 大韓消化器病學會 1995 大韓消化器病學會誌 Vol.27 No.3

해양에서 분리한 Bacillus subtilis SH-1이 분비하는 용균효소의 정제 및 특성

진성현,정영기,류병호 동의대학교 기초과학연구소 1997 基礎科學硏究論文集 Vol.7 No.1

The Research Institute for Basic Sciences DongEui UniversityThe Research Institute for Basic Sciences DongEui University

가정과 교육에 대한 남녀 중학생의 인식과 관련변수

류상희,유안진 한국 가정과 교육 학회 1993 한국가정과교육학회지 Vol.5 No.-

The purpose o this study was to investigate middle school students's perception about Home Economics in order to provide the basic materials to improve Home Economics curriculum. For this purpose reviewing literatures and empirical research were conducted. The subjects were 661 of fourteen-year-old(8th graders) enrolled in middle school in Seoul and Gyeongsang-nam-do. Statistical methods adopted for data analysis were frequencies, mean, standard deviation, ONEWAY ANOVA, Chi-square. Major findings were as follows: 1. The middle school student's perception about Home Economics Education differed with sex of subjects. That is, girl students' perception was higher than boy students' perception. 2. The middle school students' perception about Home Economics subject varied across parents' education level. 3. There was no significant difference in the middle school students' perception about Home Economics Education according to existence of mother's Job. 4. There was a significant difference in the middle school students' perception about Home Economics Education according to region. That is, the perception about the contents of eating life role was high in rural and the daily life as a consumer and resources application was high in Seoul. 5. The middle school students' perception about Home Economics Education varied across subject distinction. That is, the perception of boy students who chose Industrial Arts·Home Economics subject was high.