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Isolation and Characterization of a Lectin from the Mussel, Mytilus edulis (I)
박종옥,김희숙,Park, Jong-Ok,Kim, Hee-Sook 생화학분자생물학회 1987 한국생화학회지 Vol.20 No.3
진주담치의 homogenate로부터 음이온교환수지와 친화력 크로마토그라피를 이용하여 렉틴성분을 분리정제하였다. 이 렉틴성분은 전기영동상에서 주변 하나의 띠로 나타났으며 분자량은 약 18,000 dalton이었고 monomer로 생각된다. 이 렉틴은 토끼 적혈구를 강하게 응집시켰으며 사람혈액형에 대하여는 특이성이 낮았고 그중에서 A형 적혈구를 잘 응집시켰다. 이 렉틴의 적혈구응집활성은 galactose 및 galactose의 유도체에 의해 저해되었으며 EDTA, $Hg^{++}$및 $Mg^{++}$ 등의 양이온에 의해서도 저해되었다. 이 렉틴은 당을 함유하지 않은 단백질로 나타났으며 열에 불안정하였다. A lectin from the mussel, Mytilus edulis, was isolated and purified by using DEAE-cellulose and affinity chromatography on N-acetyl-D-galactosamine-Agarose. The purification acheived was 6,628 fold from 20,000 g homogenate with a yield of 58.1%. Its isoelectric point was around pH 7.8 and its molecular weight was estimated to be about 18,000 daltons by Sephadex G-75 gel filtration and SDS-polyacrylamide gel electrophoresis. The mussel lectin was stable below $35^{\circ}C$. The lectin agglutinated rabbit erythrocytes strongly and showed the broad specificity to human blood groups. The hemagglutinating activity of the lectin was inhibited by sugars; galactose and its derivatives, and cations; EDTA, $Hg^{++}$, and $Mg^{++}$. This lectin seemed to have no carbohydrates.
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가자(Terminalia chebula) 추출물이 마우스의 생리활성에 미치는 영향
박종옥,이승은 한국생명과학회 2004 생명과학회지 Vol.14 No.1
가자 추출물을 취하여 사람, 돼지, 쥐 및 개 등의 3% 적혈구용액으로 응집력시험을 행한 결과 7가지 적혈구 모두에 렉틴 활성이 나타났으며 $LD_{50}$는 390 mg/kg으로 측정되었다. 생리 활성에 대한 영향을 알아보고자 생체 내외인성 요인에 의한 친전자성 물질로 생체내에서 독작용, 노화, 발암 및 면역 억제작용을 유발하는 원인 물질인 free radical생성에 관여하는 효소인 XO 및 AO의 활성을 측정 한 결과, XO 에서는 일주일간 매일 300 mg/kg의 용량으로 가자 추출물을 투여한 군이 정상군보다 5배 증가되는 결과를 나타내었고 AO에서는 정상군보다 시료 투여군이 2배 증가되는 결과를 나타내었다 glutathione은 단백질이나 DNA합성, amino acid의 이동 반응 및 thiol기의 저장 등과 같은 생물학적으로 중요한 여러 가지 반응에 직접 관여하는 물질이다[16〕. 이에 간장 조직의 glutathione농도를 측정한 결과 간장 독성의 유발로 인한 효소 활성은 정상군에 비하여 1주간 매일 300 mg/kg의 용량으로 시료 투여한 군의 효소 활성이 79% 감소됨을 볼 수 있었다. 체내의 여러 가지 해독반응과정에 관여하는 GST효소활성을 측정 한 결과 정상군에 비해 1주간 매 일 300 mg/kg의 용량으로 시료 투여한 군이 66%정도 감소된다는 결과를 볼 수 있었다. glutathione 합성에 관여하는 $\gamma$-GCS의 활성과 산화형 glutathione을 환원형 glutathione으로 환원시키는 GR의 활성을 관찰한 결과 가자 추출물 투여군이 정상군보다 GR의 활성은 80% 감소되었고, 합성에 관여하는 $\gamma$-GCS의 활성은 정상군과 비교할 때 약간의 감소만을 나타내 glutathione함량 변동에 크게 영향을 미치지 않는 것으로 생각된다. In this study, we investigated the effect of water, extract of Terminalia Chebula (TC) on physiological activity in mice. TC water extract showed hemagglutination against several different types of red blood cells. $LD_{50}$ of TC extract was 390 mg/kg (po). Treatment of TC water extract orally administered 200, 300 mg/kg daily for one week. Hepatic cytosolic enzymes, xanthine oxidase and aldehyde oxidase activities were significantly increased comparison with normal group. Treatment of TC water extract increased hepatic malondialdehyde (MDA) formation, and reduced glutathione content. We also found that the decreased activities of glutathione S-transferase and glutathione reductase but was not affected activities of $\gamma$-glutamylcysteine synthetase after treatment of TC water extract. These results suggested that increase of the hepatic lipid peroxide is caused by glutathione reduction.
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박종옥 慶星大學校 1998 論文集 Vol.19 No.1
Among protein methyltranferases, protein methylase I(PM I) was found in significant amounts in human cerebrospinal fluid(CSF) samples. Both myelin basic protein(MBP) -specific and histone-specific PM I activities in CSF of neurological patients were observed, the latter being generally higher in the most CSF. MBP in the CSF of patients with multiple sclerosis(MS), optic neuritis (OD) and vasious neurological diseases was significantly higher in the MS group than in the other groups of patients , while no statistical difference was found between the MS patients with acute exacerbation and those in remission. The concentratipons of PM I products, N?, N'?-dimenthylarginine[Me₂(sym) Arg] N?,N?? -dimethylarginine [Me₂(asy)] Arg] in the urine samples from MS and control subjects was found in approximately equal amounts of both dimethylarginine isomers, Arg concentration nearly half of Me₂Arg, and the undetectable amount of N?-monomethylargineine. The urinary excretion of Me₂(asym)Arg and Me₂(sym)Arg from MS dnd control were analyzed: the values from the sample were approximately 20%(for all MS) and 33%(for chronic progressive MS) lower than those from the control for both dimethylrginine derivatives when compared to the respective compounds.
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난용성 유기 화합물의 용해, 확산 및 생분해에 대한 Rhamnolipid (Biosurfactant)의 영향
박종옥,김경순 경성대학교 2001 論文集 Vol.22 No.1
Pseudomonas aeruginosa rhamnolipid biosurfacnant enhanced solubility, dispersion, and biodegradation of organic compunds with limited water solubility. Rhamnolipid-enhanced organic compound dispersion was found to be dependant on biosurfactant concetration, pH, incubation time, structure of surfactant. All surfactants reduce the surface tension of a liquid medium. The surface tension of rhamnolipid soution was quite sensitive to pH. Organic compounds dispersion in the presence of rhamnolipids was also influenced by pH. Rhamnolipidbiosurfacnant enhanced the aqueous dispersion of octadecane by more than 4 orders of magnitude, from 0.009 to 320 ㎎/liter. Mineralization rates can be increased significantly by rhamnolipid-enhanced octadecane dispersion. The fourfold increase in mineralization was not nearly as high as the 〉104-fold increase in the aqueous dispersion of octadecane. Therefore, although rhamnolipid increase in the dispersion of octadecane, the octadecane is still not freely bioavailable. These results indicate rhamnolipids may have potential to facilitating the biodegradation of hydrocarbons in contaminated soil as well as aqueous environments.
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