정신분열병 증상을 동반한 진행성 핵상 마비 1례

박종익,조영래,홍진표,이민규,한오수,이명종 대한신경정신의학회 2001 신경정신의학 Vol.40 No.1

저자들은 환청과 망상을 주소로 정신과를 방문한 진행성 핵상 마비 환자를 진단하고 치료하였다. 진행성 핵상 마비는 보행 장해, 치매, 수직성 안구 운동의 장해가 특징적인 증상이며, 아직까지 특별한 치료법은 알려져 있지 않다. 정신과적 증상으로는 인지기능의 장해, 정동 및 행동의 변화, 수면 장해, 정신병적 증상 등이 있으며 정신분열병과 비슷한 증상을 보이는 경우는 극히 드물다고 한다. 본 증례에서는 파킨슨 증상과 정신병적 증상이 비슷한 시기에 발현된 진행성 핵상 마비의 예를 보고하면서 진단적 중요성을 강조하고자 한다. We have experienced a rare case of progressive supranuclear palsy with hallucination and delusion. The common manifestations of progressive supranuclear palsy are gait disturbance, mental change and sign of vertical ophthamoplegia but no efficacious therapy has been known. Neuropsychiatric symptom clusters include cognitive impairment, affective and behavioral changes, sleep disturbance, and psychotic symptoms. Though schizophrenia-like psychosis has been reported but this is certainly rare. Addressing a case of progressive supranuclear palsy, in whom parkisonian symptoms appeared with concurrent psychotic symptoms, we emphasize accurate diagnosis.

아스파르트산 킬레이트 철분의 철분 결핍쥐에서의 생물학적 유용성

박명규(Myoung-Gyu Park),하태열(Tae-Yul Ha),신광순(Kwang-Soon Shin) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.12

본 연구에서는 아스파르트산, 탄산칼슘 및 황산철을 반응 시켜 아스파르트산 킬레이트 철분(aspartic acid chelated iron, Asp-Fe)을 제조하고 Asp-Fe의 철분결핍 쥐(iron-deficient rat, ID)에서의 생물학적 유용성을 확인하였다. 시험군은 철분이 함유된 식이를 섭취한 정상군(NC), 철분 결핍 식이를 1개월간 투여하여 철분 결핍 상태를 유도한 쥐(ID)에 생리식염수를 공급한 결핍 대조군(ID+C), 철분 결핍 쥐에 햄철(heme-Fe) 투여군(ID+heme-Fe) 및 Asp-Fe 투여군 (ID+Asp-Fe)으로 나누어 실시하였다. 그 결과 식이섭취량, 장기무게, 체중증가 정도에서 각 군에 따른 차이가 없는 것으로 나타났다. 7일간 투여 후 혈액 중 철분의 함량을 측정한 결과 결핍쥐에 Asp-Fe 투여군(175.2 μg/dL)과 heme-Fe 투여군(140.8 μg/dL)은 결핍 대조군(96.1 μg/dL)보다 유의적인 수준으로 증가하였다. 총 철분 결합능(total iron binding capacity, TIBC)를 측정한 결과 Asp-Fe 투여군(735.4 μg/ dL)은 결핍 대조군(841.9 μg/dL)보다 유의적 수준으로 정상화되었다. 헤마토크리트(HCT) 수치를 측정한 결과에서 Asp- Fe 및 heme-Fe 모두 결핍 대조군보다 증가하는 경향은 보였지만 유의적인 차이는 없었다. 흡수율에서는 heme-Fe의 경우 21.3%인 반면에 Asp-Fe의 경우 50.2%로 약 2.3배 높은 것으로 나타났으며, 혈청에서의 철분농도 및 transferrin saturation( TS)는 heme-Fe 투여군 및 결핍 대조군에 비하여 Asp-Fe 투여군이 유의하게 높은 수준을 유지하였다. 이상의 결과로 미루어 볼 때 아미노산 킬레이트 철분은 heme-Fe과 유사한 수준의 생체 이용율을 가지고 있으며, 철분 결핍을 회복시키는데 매우 효과적인 보충제로서 이용될 수 있을 것으로 사료된다. Aspartic acid chelated iron (Asp-Fe) was synthesized by a new method using calcium carbonate, aspartic acid, and ferrous sulfate. This study was carried out to investigate the bioavailability of Asp-Fe in iron-deficient rats. We divided the rats into four experimental groups. The first was the normal diet control group, or NC. The second was the no treated control group of iron-deficient (ID) rats, or ID+C. The third was the heme-iron (heme-Fe) treated group of ID rats, ID+heme-Fe. And the fourth was the Asp-Fe treated group of ID rats, or ID+Asp-Fe. There were no differences among any of the experimental groups in diet consumption, change of body weight, or the weight of the livers, kidneys, or spleens. After 7 days of feeding, the iron content in the sera of the ID+Asp-Fe group (175.2 μg/dL) and the ID+heme-Fe group (140.8 μg/dL) were significantly higher than that of the ID-C group (96.1 μg/dL). The total iron binding capacity (TIBC) of the ID+Asp-Fe group (735.4 μg/dL) was significantly normalized compared to the ID+C group (841.9 μg/dL) or ID+heme-Fe group (824.6 μg/dL). The hematocrit level of the ID+Asp-Fe group was increased to normal levels, but there was no statistical difference among ID groups. The absorption ratio of heme-Fe was 21.3% and that of Asp-Fe was 50.2%, which indicates a 2.3 times higher ratio in comparison with heme iron. With the above results we found that Asp-Fe seems to be an efficient form of iron to supply iron deficient rats in order to cure them of anemia. Thus, these findings suggest that aspartic acid chelated iron has the potential to serve as a functional food related to iron metabolism.

아스파르트산 킬레이트 칼슘의 칼슘 결핍쥐에서의 생물학적 유용성

박명규(Park Myoung-Gyu),하태열(Ha Tae-Yul),신광순(Shin Kwang-Soon) 韓國營養學會 2011 Journal of Nutrition and Health Vol.44 No.6

Calcium (Ca) is an essential element to maintain body homeostasis. However, many factors disturb calcium absorption. Aspartic acid chelated calcium (AAC) was synthesized by new methods using calcium carbonate and aspartic acid. This study was carried out to investigate the bioavailability of AAC in Ca-deficient rats. The experimental groups were as follows: NC; normal diet control group, CD-C; untreated control group of Ca-deficient (CD) rats, CD-CaCO3; CaCO3 treated group of CD rats, CD-AAC; AAC treated group of CD rats, and CD-SWC; and seaweed-derived Ca treated group of CD rats. The Ca content of various types of Ca was held constant at 32 mg/day, and the four CD groups were fed for 7 days after randomized grouping. Ca content in serum, urine, and feces within feeding periods were analyzed to confirm Ca absorption. Serum Ca content was significantly higher in the CD-AAC (11.24 mg/dL) and CDSWC (10.12 mg/dL) groups than that in the CD-C (8.6 mg/dL) group 2 hours following the first administration. The Ca content in feces was significantly lower in the CD-AAC (35.4 mg/3 days) and CD-SWC (71.1 mg/3 day) groups than that in the CD-CaCO3 (98.7 mg/3 days) group (p > 0.05). AAC had a 2.3-fold higher absorption rate of Ca than that of SWC. No differences in fibula length were observed in the NC and CD groups. The fibula weights of the CD-AAC (0.33 g) and CD-SWC (0.33 g) groups increased compared to those in the CD-C (0.27 g) group; however, no significant difference was observed between the CD groups. We conclude that bioavailability of AAC is higher than that of seaweed-derived Ca or inorganic Ca. Thus, these findings suggest the AAC has potential as a functional food material related to Ca metabolism. (Korean J Nutr 2011; 44(6): 474~480)

자동차 앞 유리의 mist 생성 방지를 위한 자동제어

박종명(Jong-Myoung Park),김남훈(Nam-Hoon Kim),한명철(Myung-Chul Han),박원규(Warn-Gyu Park),장기룡(Ki-Lyong Jang) 대한기계학회 2002 대한기계학회 기타 간행물 Vol.2002 No.11

전염성 조혈기 괴사 바이러스 Staphylocccus epidermidis 의 생물학적 및 생화학적 특성

손상규,박정우,박명애,정영기 한국어병학회 1994 한국어병학회지 Vol.7 No.1

우리나라에 존재하며 외국에서 분리된 것과 다른 특성을 보이는 IHNV를 대상으로 하여 이 바이러스의 면역유도단백질을 확인하고자 하였다. 먼저 우리나라에서 분리된 4종류의 IHNV를 미국에서 분리된 3종류의 IHNV(OSV, SRCV 및 RB-76)와 SDS-PAGE상에서 구조단백질의 크기와 혈청학적 특성 등을 비교하였다. 그 결과 우리나라에서 분리된 4종류의 IHNV중 2종류(PRT, MRT)의 IHNV는 미국에서 분리된 IHNV와 차이가 있었으며 (Park et al., 1993), IHNV-PRT를 대상으로 면역유도단백질을 확인하기 위해 IHNV-PRT에 대한 monoclonal antibodies(MAbs)를 만들었다. 이들 중 4종류의 hybridoma를 선택하여 hybridoma cell들이 분비하는 MAbs가 어떤 class인지를 ELISA 실험을 통하여 확인한 결과 4종류 모두 IgG class에 속하는 것으로 확인되었다. 이와같이 만들어진 4종류의 MAbs가 IHNV-PRT 구조단백질들 중 어떤 것에 대한 것인지를 western blotting 실험을 통해 확인한 결과, 2종류의 MAbs는 G단백질과 특이성이 있는 것들이었고, 나머지 2종류는 G보다 조금 큰 size의 단백질에 대한 것들이었다. 다음은 IHNV-PRT에 감염된 무지개송어의 혈청을 뽑아 여기에 존재하는 IHNV-PRT에 대한 항체를 western blotting 방법으로 분석을 한 결과 G, M₁, M₂ 및 G 보다 조금 큰 size의 단백질에 대한 항체가 존재하는 것으로 나타났다. 이상의 결과로부터 IHNV-PRT의 구조단백질들 중 G, M₁, M₂ 및 G 보다 조금 큰 size의 단백질들이 면역 유도 특성이 있음을 확인할 수 있었다. To identify the immunogens of a PRT strain of Infectious Hematopoietci Necrosis Virus (IHNV) isolated from cultrued fish in Korea (Park et al, 1993). a panel of 4 monoclonal antibodies (MAbs) against IHNV-PRT strain and two polyclonal antisera from rainbow trout survived IHN disease were prepared. Proteins of purified IHNV-PRT strain were analysed on 10% SDS-PAGE and transferred onto NC paper and were incubated with the antibody solutions. With the polyclonal antibodies, four bands (M₁, M₂, G and 90Kd) were detected and the band density was in the order of M₂>90Kd>M₁>G. However, with the MAbs, only two bands(G and 90Kd) were detected. The origin of 90Kd protein was not clear but maybe cell. All the results represented that among the five proteins of IHNV-PRT strain (Park et al., 1993), M₂, M₁ and G proteins were immunogens and M₂ protein was the strongest one.

자동차 앞 유리의 mist 생성 방지를 위한 자동제어

박종명(Jong-Myoung Park),김남훈(Nam-HoonKim),한명철(Myung-ChulHan),박원규(Warn-Gyu Park),장기룡(Ki-Lyong Jang) 한국자동차공학회 2002 한국자동차공학회 Symposium Vol.2002 No.11

This paper describes the development of the automatic control device for demisting. The control is started from<br/> the mist possibility (MP) 0, Which is defined as the temperature difference between dew point and glass surface<br/> temperature.<br/> To find the efficient automatic demisting strategy, the changes of MP value in terms of interior and exterior<br/> temperature and relative humidity have been experimentally performed.<br/> Based on these experiments, the suitable automatic demisting control unit has set up and it has been found that the<br/> developed automatic demisting control unit could effectively eliminate the mist on the glass.

한국에서 분리된 전염성 조혈괴저바이러스의 M 단백질의 유전자 클로닝과 염기서열 분석

박정민,김현주,문창훈,조화자,차승주,윤원준,박정재,이은희,박명애,손상규,박정우,Park, Jeong-Min,Kim, Hyun-Ju,Mun, Chang-Hoon,Cho, Wha-Ja,Cha, Seung-Ju,Yoon, Won-Joon,Park, Jeoug-Jae,Lee, Eun-Hee,Park, Myoung-Ae,Sohn, Sang-Gyu,Park, Jeon 한국미생물학회 1998 미생물학회지 Vol.34 No.1

한국에서 분리된 전염성 조혈괴저바이러스(infectious hematopoietic necrosis virus, IHNV)인 IHNV-PRT의 특성을 규명하기 위하여 IHNV-PRT의 matrix 단백질인 M1 및 M2를 암호화하고 있는 cDNA를 클로닝하여 이들의 염기서열을 분석하였다. M1은 693 bp 크기의 open reading frame을 포함하였으며 이로부터 230개의 아미노산으로 구성된 25.9 kDa의 분자량을 가진 단백질이 합성될 수 있다. M2는 588 bp 크기의 open reading frame을 지니고 있으며 195개의 아미노산으로 구성된 21.9 kDa의 분자량을 지닌 단백질이 합성될 수 있다. IHNV-PRT의 M1과 M2의 아미노산 서열을 외국에서 분리된 IHNV들과 비교 분석한 결과 M1은 92-93%, M2는 97%의 상동성을 보였다. 이러한 사실은 IHNV의 M 단백질 유전자들은 IHNV의 strain에 관계없이 매우 보존되어 있음을 나타내준다. In order to identify the characteristics of a Korean isol ate of infectious hematopoietic necrosis virus(IHNV), IHNV-PRT, we have cloned and analyzed cDNAs coding for matrix protein M1 and M2 of the IHNV-PRT. The Ml gene contained 693 bp open reading frame and encoded a protein of 230 amino acids with a molecular weight of 25.9 kDa. The M2 gene had 588 bp open reading frame, encoding a protein of 195 amino acids with a molecular weight of 21.9 kDa. On the deduced amino-acid sequences, M1 and M2 of the IHNV-PRT were found to be 92-93% (M1) and 97% (M2) identical to those of foreign isolates of IHNV. These results indicate that M genes of the IHNV are highly conserved among different strains of IHNV.

Prediction of fracture behavior in hole expansion test using microstructure based dual-scale model

Park, Siwook,Jung, Jinwook,Kim, Sung II,Seo, Seok-Jong,Lee, Myoung-Gyu,Han, Heung Nam Institute of Physics Publishing Ltd 2018 Journal of Physics: Conference Series Vol.1063 No.-

전염성 조혈기 괴사 바이러스(IHNV)의 항원 유도 단백질 특성

박명애,손상규,박정우,정영기 동의대학교 기초과학연구소 1995 基礎科學硏究論文集 Vol.5 No.1

To identify the immunogens of a PRT strain of Infectious Hematopoietci Necrosis Virus(IHNV) isolated from cultrued fish in Korea (Park et al., 1993), a panel of 4 monoclonal antibodies (MAbs) against IHNV-PRT strain and two polyclonal antisera from rainbow trout survived IHN disease were prepared. Proteins of purified IHNV-PRT strain were analysed on 10% SDS-PAGE and transferred onto NC paper and were incubated with the antibody solutions. With the polyclonal antibodies, four bands (M₁, M₂, G and 90Kd) were detected and the band density was in the order of M₂$gt;90Kd$gt;M₁$gt;G. However, with the MAbs, only two bands(G and 90Kd) were detected. The origin of 90Kd protein was not clear but maybe cell. All the results represented that among the five proteins of IHNV-PRT strain (Part et al., 1993). M₂, M₁ and G proteins were immunogens and M₂ protein was the strongest one.

Synthesis, characterization, and biocompatible properties of alanine-grafted chitosan copolymers

Park, Gyu Han,Kang, Min-Sil,Knowles, Jonathan C,Gong, Myoung-Seon SAGE Publications 2016 Journal of biomaterials applications Vol.30 No.9

<P>In order to overcome major problems regarding the lack of affinity to solvents and limited reactivity of the free amines of chitosan, introduction of appropriate spacer arms having terminal amine function is considered of interest. L-Alanine-N-carboxyanhydride was grafted onto chitosan via anionic ring-opening polymerization. The chemical and structural characterizations of L-alanine-grafted chitosan (Ala-g-Cts) were confirmed through Fourier transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy (H-1 NMR). In addition, the viscoelastic properties of Ala-g-Cts were examined by means of a rotational viscometer, and thermal analysis was carried out with a thermogravimetric analyzer and differential scanning calorimetry. Morphological changes in the chitosan L-alanine moiety were determined by x-ray diffraction. To determine the feasibility of using these films as biomedical materials, we investigated the effects of their L-alanine content on physical and mechanical properties. The biodegradation results of crosslinked Ala-g-Cts films were evaluated in phosphate-buffered solution containing lysozyme at 37?. Proliferation of MC3T3-E1 cells on crosslinked Ala-g-Cts films was also investigated with use of the CCK-8 assay.</P>