FSCB phosphorylation in mouse spermatozoa capacitation

( Shun Li Liu ),( Bing Ni ),( Xiang Wei Wang ),( Wen Qian Huo ),( Jun Zhang ),( Zhi Qiang Tian ),( Ze Min Huang ),( Yi Tian ),( Jun Tang ),( Yan Hua Zheng ),( Feng Shuo Jin ),( Yan Feng Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.8

It is generally accepted that spermatozoa capacitation is associated with protein kinase A-mediated tyrosine phosphorylation. In our previous study, we identified the fibrous sheath CABYR binding protein (FSCB), which was phosphorylated by PKA. However, the phosphorylation status of FSCB protein during spermatozoa capacitation should be further investigated. To this aim, in this study, we found that phosphorylation of this 270-kDa protein occurred as early as 1 min after mouse spermatozoa capacitation, which increased over time and remained stable after 60 min. Immunoprecipitation assays demonstrated that the tyrosine and Ser/Thr phosphorylation of FSCB occurred during spermatozoa capacitation. The extent of phosphorylation and was closely associated with the PKA activity and spermatozoa motility characteristics. FSCB phosphorylation could be induced by PKA agonist DB-cAMP, but was blocked by PKA antagonist H-89.Therefore, FSCB contributes to spermatozoa capacitation in a tyrosine-phosphorylated format, which may help in further elucidating the molecular mechanism of spermatozoa capacitation. [BMB reports 2011; 44(8): 541-546]

Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus

Tian-Min Qiao,Jing Zhang,Shu-Jiang Li,Shan Han,Tian-Hui Zhu 한국식물병리학회 2016 Plant Pathology Journal Vol.32 No.5

Eucalyptus dieback disease, caused by Cylindrocladiumscoparium, has occurred in last few years in largeEucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniquesare desired for the early detection of Eucalyptus diebackof C. scoparium prior to formulation of efficientcontrol plan. For this purpose, three PCR-based methodsof nested PCR, multiplex PCR, loop-mediatedisothermal amplification (LAMP) were developed fordetection of C. scoparium based on factor 1-alpha (tef1)and beta-tubulin gene in this study. All of the threemethods showed highly specific to C. scoparium. Thesensitivities of the nested PCR and LAMP were muchhigher than the multiplex PCR. The sensitivity of multiplexPCR was also higher than regular PCR. C. scopariumcould be detected within 60 min from infectedEucalyptus plants by LAMP, while at least 2 h wasneeded by the rest two methods. Using different Eucalyptustissues as samples for C. scoparium detection,all of the three PCR-based methods showed muchbetter detection results than regular PCR. Base on theresults from this study, we concluded that any of thethree PCR-based methods could be used as diagnostictechnology for the development of efficient strategiesof Eucalyptus dieback disease control. Particularly,LAMP was the most practical method in field applicationbecause of its one-step and rapid reaction, simpleoperation, single-tube utilization, and simple visualizationof amplification products.

Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus

Qiao, Tian-Min,Zhang, Jing,Li, Shu-Jiang,Han, Shan,Zhu, Tian-Hui The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.5

Eucalyptus dieback disease, caused by Cylindrocladium scoparium, has occurred in last few years in large Eucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniques are desired for the early detection of Eucalyptus dieback of C. scoparium prior to formulation of efficient control plan. For this purpose, three PCR-based methods of nested PCR, multiplex PCR, loop-mediated isothermal amplification (LAMP) were developed for detection of C. scoparium based on factor 1-alpha (tef1) and beta-tubulin gene in this study. All of the three methods showed highly specific to C. scoparium. The sensitivities of the nested PCR and LAMP were much higher than the multiplex PCR. The sensitivity of multiplex PCR was also higher than regular PCR. C. scoparium could be detected within 60 min from infected Eucalyptus plants by LAMP, while at least 2 h was needed by the rest two methods. Using different Eucalyptus tissues as samples for C. scoparium detection, all of the three PCR-based methods showed much better detection results than regular PCR. Base on the results from this study, we concluded that any of the three PCR-based methods could be used as diagnostic technology for the development of efficient strategies of Eucalyptus dieback disease control. Particularly, LAMP was the most practical method in field application because of its one-step and rapid reaction, simple operation, single-tube utilization, and simple visualization of amplification products.

Raman Lidar for the Measurement of Temperature, Water Vapor, and Aerosol in Beijing in the Winter of 2014

Min Tan,Zhen Shang,Chenbo Xie,Hui Ma,Qian Deng,Xiaomin Tian,Peng Zhuang,Zhanye Zhang,Yingjian Wang 한국광학회 2018 Current Optics and Photonics Vol.2 No.1

To measure atmospheric temperature, water vapor, and aerosol simultaneously, an efficient multi-function Raman lidar using an ultraviolet-wavelength laser has been developed. A high-performance spectroscopic box that utilizes multicavity interference filters, mounted sequentially at small angles of incidence, is used to separate the lidar return signals at different wavelengths, and to extract the signals with high efficiency. The external experiments are carried out for simultaneous detection of atmospheric temperature, water vapor, and aerosol extinction coefficient in Beijing, under clear and hazy weather conditions. The vertical profiles of temperature, water vapor, and aerosol extinction coefficient are analyzed. The results show that for an integration time of 5 min and laser energy of 200 mJ, the mean deviation between measurements obtained by lidar and radiosonde is small, and the overall trend is similar. The statistical temperature error for nighttime is below 1 K up to a height of 6.2 km under clear weather conditions, and up to a height of 2.5 km under slightly hazy weather conditions, with 5 min of observation time. An effective range for simultaneous detection of temperature and water vapor of up to 10 km is achieved. The temperature-inversion layer is found in the low troposphere. Continuous observations verify the reliability of Raman lidar to achieve real-time measurement of atmospheric parameters in the troposphere.

The conception about Gods of Chinese Traditional Culture : According to the Thought of Gods Will Being Included in

Tian-min Wang 한국마음학회 2013 한국마음학회 학술대회논문집 Vol.2013 No.1

Confucianism is the major school of Chinese traditional culture. Lun Yu as the foundational and dominant literature of Confucianism represents the particularity and the basic idea of Chinese traditional culture. Abundant thought of Gods will included in Lun Yu provides unquestionable evidence and valuable resources for exploring and analyzing the conception about Gods of Chinese traditional culture. The Gods ontology which is the trinity of mind, Gods will and Gods, the function of Gods that different Gods are responsible for the different transactions, and the psychic concept performed by exceptional people or in special moment that show in Lun Yu have a profound impact on contemporary Chinese Gods concept and the awareness of beliefs. Therefore, researching the conception about Gods of Chinese traditional culture based on the thought of Gods included in Lun Yu has important significance both in theory and practice.

The preservation effect of Metschnikowia pulcherrima yeast on anthracnose of postharvest mango fruits and the possible mechanism

Tian, Ya-qin,Li, Wen,Jiang, Zi-tao,Jing, Min-min,Shao, Yuan-zhi 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.1

This study aimed to determine the effects of Metschnikowia pulcherrima yeast on storage quality of 'Tainong' mango, and elucidate it's possible anti-disease mechanism. The results showed that M. pulcherrima could inhibit the changes in peel colour, fruit firmness, the contents of total soluble solids, total acid and vitamin C, and maintain the storage quality of mango fruits. An investigation of the mechanism showed that M. pulcherrima competed not only for the primary carbon source, but also for living space with Colletotrichum gloeosporioides. In addition, M. pulcherrima promoted the activities of defence-related enzymes, including ${\beta}$-1,3-glucanase(GLU) and chitinase (CHT), and secreted a small amount of antimicrobial substances composed of volatile and nonvolatile anti-fungal compounds. The results strongly demonstrated that antagonistic yeast M. pulcherrima could be applied as a biocontrol agent for deducing the spoilage and decay of mango fruit.

Simultaneous quantitation and validation of method for the quality evaluation of Eucommiae cortex by HPLC/UV

( Bing Tian Zhao ),( Su Yang Jeong ),( Tae In Kim ),( Eun Kyoung Seo ),( Byung Sun Min ),( Jong Keun Son ),( Mi Hee Woo ) 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-

A new HPLC/UV method has been developed for the simultaneous quantitative determination of four major components in Eucommiae cortex, namely geniposidic acid (1), geniposide (2), pinoresinol di-O-β-D-glucopyranoside (3), and liriodendrin (4). Simultaneous separations of these four components were achieved on a J``sphere ODS CI8 column (250 x 4.6 mm, 4 mm), The elution was done using water with 0.1 % phosphoric acid (A) and acetonitrile with 0.1 % phosphoric acid (B) in a two-step elution of the mobile phase at a flow rate of 1.0 mL/min and a wavelength of 230 nm. The method was validated for linearity, recovery, precision, accuracy, stability and robustness. All calibration curves showed good linear regression (r² > 0.999) within the test ranges. This method showed good recovery and repro-ducibility for the quantification of these four components in 85 species of Eucommiae cortex. The intra-day and inter-day precisions were lower than 0.53 % (as a relative standard deviation, RSD) and accuracies between 93.00 and 106.28 % for all standards. The results indicate that the established HPLC/UV method is suitable for quantitation and quality evaluation of Eucommiae cortex.

Dynamic analysis method for the progressive collapse of long-span spatial grid structures

Li-Min Tian,Jian-peng Wei,Ji-Ping Hao,Xian-tie Wang 국제구조공학회 2017 Steel and Composite Structures, An International J Vol.23 No.4

In the past, the progressive collapse resulting from local failures during accidents has caused many tragedies and loss of life. Although long-span spatial grid structures are characterised by a high degree of static indeterminacy, the sudden failure of key members may lead to a catastrophic progressive collapse. For this reason, it is especially necessary to research the progressive collapse resistance capacity of long-span spatial grid structures. This paper presents an evaluation method of important members and a novel dynamic analysis method for simulating the progressive collapse of long-span spatial grid structures. Engineering cases were analysed to validate these proposed method. These proposed methods were eventually implemented in the progressive collapse analysis of the main stadium for the Universiade Sports Center. The roof of the structure was concluded to have good resistance against progressive collapse. The novel methods provide results close to practice and are especially suitable for the progressive collapse analysis of long-span spatial grid structures.

Simultaneous analysis of seven marker compounds from Saposhnikoviae Radix, Glehniae Radix and Peucedani Japonici Radix by HPLC/PDA

U. Min Seo,Bing Tian Zhao,김영호,Jong Seong Kang,Jong Keun Son,Mi-Hee Woo 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.5

A new combination of high performance liquidchromatography (HPLC) method coupled with photodiodearray (PDA) analysis has been developed for the simultaneousquantitative determination of seven major components inSaposhnikoviae Radix (SR), Glehniae Radix (GR) and PeucedaniJaponici Radix (PR), namely peucedanol 7-O-b-Dglucopyranoside(1), prim-O-glucosylcimifugin (2), cimifugin(3), 40-O-b-D-glucosyl-5-O-methylvisamminol (4), bergapten(5), sec-O-glucosylhamaudol (6), and imperatorin (7). Clearseparation of these seven components were achieved on aPhenomenex Kinetex C18 (250 9 4.6 mm, 5 lm) column bygradient elution of water (A) and methanol (B) as mobilephase. The flow rate was 1.0 mL/min and the UV detectorwavelength was set at 254 nm. The method was successfullyused in the analysis of SR, GR, and PR with relatively simpleconditions and procedures, and the results were satisfactory forlinearity, recovery, precision, accuracy, stability and robustness. The results indicate that the established HPLC/PDAmethod is suitable for the classification of SR, GR, and PR.

Knockdown of Chloride Channel-3 Inhibits Breast Cancer Growth In Vitro and In Vivo

Fang-Min Zhou,Yun-Ying Huang,Tian Tian,Xiao Yan Liu,Yong-Bo Tang 한국유방암학회 2018 Journal of breast cancer Vol.21 No.2

Purpose: Chloride channel-3 (ClC-3) is a member of the chloride channel family and plays a critical role in a variety of cellular activities. The aim of the present study is to explore the molecular mechanisms underlying the antitumor effect of silencing ClC-3 in breast cancer. Methods: Human breast cancer cell lines MDAMB- 231 and MCF-7 were used in the experiments. Messenger RNA and protein expression were examined by quantitative realtime polymerase chain reaction and western blot analysis. Cell proliferation was measured by the bromodeoxyuridine method, and the cell cycle was evaluated using fluorescence-activated cell sorting. Protein interaction in cells was analyzed by co-immunoprecipitation. Tumor tissues were stained with hematoxylin- eosin and tumor burden was measured using the Metamorph software. Results: Breast cancer tissues collected from patients showed an increase in ClC-3 expression. Knockdown of ClC-3 inhibited the secretion of insulin-like growth factor (IGF)-1, cell proliferation, and G1/S transition in breast cancer cells. In the mouse xenograft model of human breast carcinoma, tumor growth was significantly slower in animals injected with ClC- 3-deficient cells compared with the growth of normal human breast cancer cells. In addition, silencing of ClC-3 attenuated the expression of proliferating cell nuclear antigen, Ki-67, cyclin D1, and cyclin E, as well as the activation of extracellular signalregulated protein kinases (ERK) 1/2, both in vitro and in vivo. Conclusion: Together, our data suggest that upregulation of ClC- 3 by IGF-1 contributes to cell proliferation and tumor growth in breast cancer, and ClC-3 deficiency suppresses cell proliferation and tumor growth via the IGF/IGF receptor/ERK pathway.