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Molecular signatures of transgenerational response to ocean acidification in a species of reef fish
Schunter, Celia,Welch, Megan ,J.,Ryu, Taewoo,Zhang, Huoming,Berumen, Michael ,L.,Nilsson, Gö,ran ,E.,Munday, Philip ,L.,Ravasi, Timothy Nature Publishing Group, a division of Macmillan P 2016 Nature climate change Vol.6 No.11
<P>The impact of ocean acidification on marine ecosystems will depend on species capacity to adapt(1,2). Recent studies show that the behaviour of reef fishes is impaired at projected CO2 levels(3,4); however, individual variation exists that might promote adaptation. Here, we show a clear signature of parental sensitivity to high CO2 in the brain molecular phenotype of juvenile spiny damselfish, Acanthochromis polyacanthus, primarily driven by circadian rhythm genes. Offspring of CO2-tolerant and CO2-sensitive parents were reared at near-future CO2 (754 mu atm) or present-day control levels (414 mu atm). By integrating 33 brain transcriptomes and proteomes with a de novo assembled genome we investigate the molecular responses of the fish brain to increased CO2 and the expression of parental tolerance to high CO2 in the offspring molecular phenotype. Exposure to high CO2 resulted in differential regulation of 173 and 62 genes and 109 and 68 proteins in the tolerant and sensitive groups, respectively. Importantly, the majority of differences between offspring of tolerant and sensitive parents occurred in high CO2 conditions. This transgenerational molecular signature suggests that individual variation in CO2 sensitivity could facilitate adaptation of fish populations to ocean acidification.</P>
Chun, Honggu,Dennis, Patty J.,Ferguson Welch, Erin R.,Alarie, Jean Pierre,Jorgenson, James W.,Ramsey, J. Michael Elsevier 2017 Journal of chromatography Vol.1523 No.-
<P>The development and application of polyelectrolytic gel electrodes (PGEs) for a microfluidic photothermal absorbance detection system is described. The PGEs are used to measure changes in conductivity based on heat generation by analytes absorbing light and changing the solution viscosity. The PGEs are suitable for direct contact conductivity measurements since they do not degrade with exposure to high electric fields. Both a 2-electrode system with DC voltages and a 3-electrode system with AC voltages were investigated. Experimental factors including excitation voltage, excitation frequency, laser modulation frequency, laser power, and path length were tested. The limits of detection for the 3-electrode and 2-electrode systems are 500 nM and 0.55 nM for DABSYL-tagged glucosamine, respectively. In addition, an electrokinetic separation of a potassium, DABSYL-tagged glucosamine, Rhodamine 6G, and Rhodamine B mixture was demonstrated. (C) 2017 Elsevier B.V. All rights reserved.</P>
CCQM K6.2 determination of total cholesterol in human serum
Wise, Stephen A,Phinney, Karen W,Duewer, David L,Sniegoski, Lorna T,Welch, Michael J,Pabello, Guiomar,Caldero, Marco A Avila,Qinde, Liu,Kooi, Lee Tong,Rego, Eliane,Garrido, Bruno,Allegri, Gabriella,Cr BUREAU INTERNATIONAL DES POIDS ET MESURES 2018 METROLOGIA -BERLIN- Vol.55 No.1