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중합효소연쇄반응을 이용한 도말양성 폐결핵 환자에서의 결핵균 DNA 검출방법에 관한 연구
이종진,김애경,조해정,한표성,홍석철,김주옥,김선영 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2
Polymerase chain reaction (PCR) is the most sensitive and rapid method for the diagnosis of M.tuberculosis infection. Many reports have shown different methods for mycobacterial DNA extraction, but revealed many differences in simplicity and time-consumption. We studied which method was the best among 7 different ones for DNA extraction from 32 smear-positive sputa, using PCR of targeting 123bp DNA fragment of IS6110. The methods were the following : SDS-Microwave oven method, NaOH Lysis method, Triton X-100, Proteinase K method, Lysis buffer method. SDS-Proteinase K method, Bead Beater method, and Bead Beater/CTAB method. The amplification products were identified by electrophoresis on ethidium bromide-stained agarose gels, and confirmed by restriction enzyme, Southern blot hybridization, and DNA sequencing. All 32 cases were positive (100%) by the 7 different methods. It took 1 hour or more to detect DNA in SDS-Microwave oven method, NaOH Lysis method, and Bead beater/CTAB method. It took 2 hours or less in the others. SDS-Proteinase K method and Bead Beater/CTAB method were simpler and more convinient than the others. These results suggest that SDS-Proteinase K method and Bead Beater/CTAB method might be better than the others for the extraction of mycobacterial DNA in terms of simplicity and time-consumption, although all the methods were sensitive.
김윤정,오영숙,표한경 이화여자대학교약학회 1992 梨花藥學硏究 Vol.- No.31
We have conducted the experiment in order to investigate the effect of estrogen on rat uterine growth and the possible mechanism of action of estrogen on rat uterine growth. The result of time course study of estrogen-17β in immature female rat uterus showed the time dependent stimulation of rat uterine growth. Also the result of dose response study with E 17β in immature rat uterus showed the dose dependent increase in the uterine weight. Monitoring the total protein from the both time course study and dose response study indicates that the increase of rat uferine weight by estrogen correlates with the increase of total protein in rat uterus. Therefore Estrogen saems to stimulate rat uterine growth via the increase of protein synthesis
Micronesia, Chuuk Lagoon에 서식하는 파랑돔(Pomacentrus coelestis)의 생식년주기
김한준 ( Han-jun Kim ),한정훈 ( Jeonghoon Han ),허성표 ( Sung-pyo Hur ),이균우 ( Kyun-woo Lee ),최영웅 ( Young-ung Choi ) 한국수산과학회(구 한국수산학회) 2021 한국수산과학회지 Vol.54 No.2
This study was conducted to investigate the reproductive characteristics of the Neon damselfish Pomacentrus coelestis in Chuuk lagoon (7°27′ N, 151°53′ E), Micronesia, Equatorial Pacific Ocean. The specimens used in this study were collected from August 2009 to September 2010 from around the coral reef area at a depth of 3-10 m in the northwest coast of Weno Island. Overall, 228 individuals were collected over 14 months from this area, of which 115 were females and 108 were males. Among 108 males, 30 were found to be hermaphrodites and the sex of 5 individuals could not be determined. The overall sex ratio was approximately 1.06:1 (female:male), and the ratio of sexually active individuals was 0.79:1. The gonadosomatic index (GSI) fluctuated and showed the highest value of 8.43±1.21 in December 2009 and the lowest value of 3.36±0.71 in September 2009 in sexually active females. Males showed the highest GSI value of 2.64±0.93 in June 2010 and the lowest value of 0.35±0.06 in July 2010. Based on the fluctuation of GSI, the trends in the appearance of monthly sexually active individuals, and biopsy of the gonads, P. coelestis was found to be a hermaphrodite species of protogynous type and is considered to be reproductively active throughout the year.
전두 탈모증 환자에서 DPCP 치료 중에 발생된 Stevens - Johnson 증후군
한경표 ( Kyoung Pyo Han ),이선영 ( Sun Young Lee ),김영조 ( Young Jo Kim ),최규철 ( Kyu Chul Choi ) 대한피부과학회 1998 대한피부과학회지 Vol.36 No.2
Topical immunotherapy with diphenylcyclopropenone(DPCP) has been used for the treatment of alopecia areata. Due to the therapeutic principle of producing a contact eczema, itching, erythema and scaling are inevitable or even desired side effects. However, erythema multiforme-like reactions following topical DPCP treatment have been rarely reported with an estimated incidence of 1.2%. We present herein a case of Stevens-Johnson syndrome, the severe form of erythema multiforme as a side effect of topical DPCP. A 57-year old male patient visited us for the treatment of alopecia totalis. After sensitization with 0.2% DPCP in acetone, we applied DPCP on the scalp once a week for three weeks. Following the 3rd challenge of DPCP, iris-shaped lesions, erosions, vesicles, and bullae developed with fever. Also, he had vesicles and erosions in the oral cavity. The patient was treated with systemic antibiotics, steroids, and antihistamines. The cutaneous lesions were cleared with hyperpigmentation, and pronounced hair regrowth was observed. (Korean J Dermatol 1998;36(2): 326-330)
한범구,김정근,백제현,이우진,윤경한,주동표,조도현 한국키틴키토산학회 1998 한국키틴키토산학회지 Vol.3 No.4
We proposed a quantitative empiric equation for the effect of reaction time and reaction temperature on chemical deacetylation of chitin. The mathematic formula was proved to be in good agreement with the experimental values within 1.94% of AAD. According to the estimated results by the equation, optimal reaction temperature and reaction time were 413-433K and 60 minutes or less, respectively.
Deep Learning Model for Smartphone-recorded Cough Sounds: A Prospective Study in Predicting Asthma
( Kyoung Min Moon ),( Hyoungshin Choi ),( Sanghoon Han ),( Jinhee Jeon ),( Choongki Min ),( Kyungnam Kim ),( Donghoon Kim ),( Myung Pyo Kim ),( Young Mi Park ) 대한결핵 및 호흡기학회 2023 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.136 No.0