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( Chan Kwon Park ),( Nahyeon Kang ),( Su Yeon Choi ),( Bit Na Kim ),( Chang Dong Yeo ),( Jin Woo Kim ),( Sang Haak Lee ),( Young Kyoon Kim ),( Dong Soo Lee ),( Tae-jung Kim ),( Seong-beom Lee ),( Sug 대한결핵 및 호흡기학회 2017 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.124 No.-
A hypoxic microenvironment leads to increase the invasiveness and the metastatic potential of cancer cells within tumors via epithelial- mesenchymal transition (EMT) and cancer stemness acquisition in multiple cancers. However, hypoxia-induced changes in expression and function of candidate stem cell markers and their possible molecular mechanism has not well been understood. Normal and cancerous lung cell lines (BEAS-2B, A549, H292, H226 and H460) were analyzed in normoxic or hypoxic (less than 1% O2) conditions. For screening of changes in expression among stem cell markers, transcriptome analysis using next-generation sequencing was performed by HiSeq 2500 system. For validation, stem cell characteristics were analyzed with RT-PCR, western blotting, immunofluorescence staining, soft agar colony and sphere formation assays, and an in vivo mouse tumor model. EMT was assessed with western blotting, as well as wound healing and Matrigel invasion assays. To determine whether epigenetic mechanism is involved, cell lines were treated with a DNA methyltransferase inhibitor (AZA), and methylation-specific PCR and bisulfite sequencing were performed. Next-generation sequencing revealed CXCR4 expression was significantly increased after hypoxic condition which functionally resulted in EMT and cancer stemness acquisition. The acquisition of EMT and stemness properties was inhibited by treatment with CXCR4 siRNA. CXCR4 was activated by either hypoxic condition and treatment with AZA.
Comparison of different impression techniques for edentulous jaws using three-dimensional analysis
Jung, Sua,Park, Chan,Yang, Hong-So,Lim, Hyun-Pil,Yun, Kwi-Dug,Ying, Zhai,Park, Sang-Won The Korean Academy of Prosthodonitics 2019 The Journal of Advanced Prosthodontics Vol.11 No.3
PURPOSE. The purpose of this study was to compare two novel impression methods and a conventional impression method for edentulous jaws using 3-dimensional (3D) analysis software. MATERIALS AND METHODS. Five edentulous patients (four men and one woman; mean age: 62.7 years) were included. Three impression techniques were used: conventional impression method (CI; control), simple modified closed-mouth impression method with a novel tray (SI), and digital impression method using an intraoral scanner (DI). Subsequently, a gypsum model was made, scanned, and superimposed using 3D analysis software. Mean area displacement was measured using CI method to evaluate differences in the impression surfaces as compared to those values obtained using SI and DI methods. The values were confirmed at two to five areas to determine the differences. CI and SI were compared at all areas, while CI and DI were compared at the supporting areas. Kruskal-Wallis test was performed for all data. Statistical significance was considered at P value <.05. RESULTS. In the comparison of the CI and SI methods, the greatest difference was observed in the mandibular vestibule without statistical significance (P>.05); the difference was < 0.14 mm in the maxilla. The difference in the edentulous supporting areas between the CI and DI methods was not significant (P>.05). CONCLUSION. The CI, SI, and DI methods were effective in making impressions of the supporting areas in edentulous patients. The SI method showed clinically applicability.
<i>In vivo</i> corrosion mechanism by elemental interdiffusion of biodegradable Mg–Ca alloy
Jung, Jae‐,Young,Kwon, Sang‐,Jun,Han, Hyung‐,Seop,Lee, Ji‐,Young,Ahn, Jae‐,Pyoung,Yang, Seok‐,Jo,Cho, Sung‐,Youn,Cha, Pil‐,Ryung,Kim, Yu‐,Chan,Seo Wiley Subscription Services, Inc., A Wiley Company 2012 Journal of biomedical materials research. Part B, Vol.b100 No.8
<P><B>Abstract</B></P><P>We elucidated the <I>in vivo</I> corrosion mechanism of the biodegradable alloy Mg–10 wt % Ca in rat femoral condyle through transmission electron microscope observations assisted by focused ion beam technique. The alloy consists of a primary Mg phase and a three‐dimensional lamellar network of Mg and Mg<SUB>2</SUB>Ca. We found that the Mg<SUB>2</SUB>Ca is rapidly corroded by interdiffusion of Ca and O, leading to a structural change from lamellar network to nanocrystalline MgO. In contrast to the fast corrosion rate of the lamellar structure, the primary Mg phase slowly changes into nanocrystalline MgO through surface corrosion by O supplied along the lamellar networks. The rapid interdiffusion induces an inhomogeneous Ca distribution and interestingly leads to the formation of a transient CaO phase, which acts as a selective leaching path for Ca. In addition, the outgoing Ca with P from body fluids forms needle‐type calcium phosphates similar to hydroxyl apatite at interior and surface of the implant, providing an active biological environment for bone mineralization. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.</P>
Colonoscopy learning curves for colorectal surgery fellow trainees
Jung Ryul Oh,Kyung Su Han,Chang Won Hong,Byung Chang Kim,Bun Kim,Sung Chan Park,Min Jung Kim,Sang Jae Lee,Jae Hwan Oh,Changha Shin,Dae Kyung Sohn 대한외과학회 2018 Annals of Surgical Treatment and Research(ASRT) Vol.95 No.4
Purpose: This study aimed to analyze the learning curves for colorectal surgery fellows in a colonoscopy training program. Methods: Between May 2003 and February 2017, 60 surgical fellows joined our 1-year colonoscopy training program as trainees and performed 43,784 cases of colonoscopy. All trainees recorded their colonoscopy experiences prospectively into the database. After excluding 6 trainees, who had experience with performing more than 50 colonoscopies before participating in our training program or who discontinued our training program with experience performing less than 300 colonoscopies, this study included 54 trainees who had performed 39,539 colonoscopy cases. We analyzed the cecal intubation rate (CIR) and cecal intubation time (CIT) using the cumulative sum (Cusum) technique and moving average method to assess the technical colonoscopy competence. Results: Overall, the CIR by the trainees was 80.7%. The median number of cases of colonoscopy performed during the training period for each trainee was 696 (range, 322–1,669). The trainees were able to achieve a 90% CIR with 412 and 493 procedures when analyzed using the moving average and the Cusum, respectively. Using the moving average method, CIRs after 150, 300, and 400 procedures were 67.0%, 84.1%, and 89.2%, respectively. The CIT of trainees continuously decreased until 400 successful cases. Median CITs were 9.4, 8.3, and 7.4 minutes at 150, 300, and 400 successful cases, respectively. Conclusion: We found that more than 400 cases of experience were needed for technical competence in colonoscopy. Continuous teaching and monitoring is required until trainees become sufficiently competent.
Chan-Jung Lee,Eun-Ji Lee,Hye-Sung Park,Won-Sik Kong 한국버섯학회 2017 버섯 Vol.21 No.1
The button mushroom, Agaricus bisporus, is one of the major economical crops cultivated in Korea. This mushroom showed the 5th production to 11,493 M/T in 2014. Several fungus are known as the causal agents of diseases of the cultivated button mushroom (Agaricus bisporus) and oyster mushroom (Pleurotus ostreatus). Trichoderma harzianum is the causal agent of green mould disease of commercial mushrooms. Early symptoms were noticed as round, fleshy, brown lesions on mushroom caps. Late symptoms spread on the surface of the casing, and covered entirely fruiting bodies. A Gram-positive bacterium was isolated from mushroom media that markedly showed the antagonistic activity against Trichoderma harzianum, the destructive pathogen of cultivated mushrooms. The CH518 strain was selected as antagonistic bacterium by inhibition zone method and it was identified as Bacillus methylotrophicus by the cultural, morphological and physiological characteristics, and analysis of the 16S rDNA. The isolated bacterium is saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for Trichoderma harzianum cell, was sufficient for inhibition in vitro. Inoculation of the isolated bacterium prevents the development of bacterial disease in Trichoderma harzianum. Control efficacy of browning disease of strain CH518 treatment was 77.7% on Agaricus bisporus. The optimal culture medium for the antagonistic bacteria growth was determined as follows: 3.0% saccharose, 1.5% Soytone, 1% NH4H2PO4, 10 mM MgSO4, and 2.0% glutamic acid at pH 6.0 at 25°C. The suppressive bacterium may be useful in future for the development of biocontrol system and the construction of genetically modified edible fungi resistant to the disease caused by Trichoderma harzianum.
Jung, Chang Hwa,Jung, Hee,Shin, Yong-Cheol,Park, Jong-Hyeong,Jun, Chan-Yong,Kim, Hyung-Min,Yim, Hee-Sun,Shin, Min-Gyu,Bae, Hyun-Soo,Kim, Sung-Hoon,Ko, Seong-Gyu WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-
Eleutherococcus senticosus (Aratiaceae) is immunological modulator which has bcen successfully used for anti-inflammatory effectors on anti-rheumatic diseases in oriental medicine. Mitogen-activated protein kinases (MAPKs) and Akt modulate the transcnption of many genes involved in the indammatory process. In this study, we investigated the inhibitory effects of Eleutherococcus senticosus on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (C0X-2) in lipopolysaccharides (LPS)-activated macrophages. Finally, we studied the involvement of MAPKs and Akt signaling in the protective effect of Eteutherococcus senticosus in LPS-aclivated macrophages. Eteutherococcus senticosus significantly attcnuated LPS-induccd iNOS cxpression but not C0X-2 expression. In using Ihe standard inhibitors (MAPKs and Akl), our results show that Eteutherococcus senticosus downregulates inflammatory iNOS expression by blocking JNK and Akt activation.