환경시료 중의 다이옥신류 분석방법에 관한 연구

김종국,신은섭 全北大學校 附設 都市및環境硏究所 1997 都市 및 環境硏究 Vol.12 No.1

An analytical method was investigated for the isomer-specific determination of tetra to octa chlorinated dibenzo-p-dioxins and dibenzofurans in environmental samples A series of column chromatographic clean up, silicagel, alumina, activated carbon, were discussed. Identification and quantification were performed using high resolution gas chromatography/high resolution mass spectrometry. The result shows that the toxicologically important 2,3,7,8-substituted isomers can be separated from other isomers with two GC columns of DB-5 and DB-17. The range of internal standard recoveries were 71.3∼91.9% with 5.7∼11.3% standard deviations for environmental samples. The detection limits were 0.01∼0.1pg/g dry weight, for semident and soil samples, and 0.01∼0.06pg/g wet weight for organism samples.

위장관 출혈을 일으킨 공장의 형활근종 1예

김병수,장성종,부귀범,조운택,박찬국,김만우,변주남,김정용,전호종 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

Small bowel leiomyoma is a rare tumor which may occur wherever smooth muscle is present. The small bowel is the most common site and it makes up 20~30% of all benign small intestinal neoplasms. A spectrum of symptoms are common ranging from nausea, vomiting, abdominal pain, to massive intestinal bleeding. Bleeding is the most common symptom and is usually gross and recurrent. Occasionally it may be life threatening. But, the most serious problem is both the absence of specific clinical symptoms, and the difficulties shown in radiological diagnostics of the small bowel. If conventional diagnostic methods fail to reveal any positive findings for gastrointestinal bleeding, the diagnosis of intestinal leiomyoma should be suspected and an SMA angiography shoud be performed. The angiographic criteria of small bowel leiomyoma is very characteristic. We present a case of leiomyoma with considerable and prolonged gastrointestinal bleeding, which was diagnosed by angiography. A 61-year old woman was admitted with a history of melena over the last 10 days. An SMA angiography showed a hypervascular mass with a diameter of 3×3cm. A segmental resection was performed under the impression of the gastrointestinal bleeding due to leiomyoma of the small bowel. Microscopic examination of the lesion shows interlacing or whorl bundles of spindle shaped ces and no mitosis.

위장관의 원발성 B 세포 악성 림프종의 재분류와 bcl-2, cyclin D1, bcl-6 및 p53 발현의 의미

서정균,김태원,김병수,조은택,박찬국,김만우,이미자,전호종 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : In recent years, the REAL (Revised European American Lymphoma) classification provided a new category of MALT (mucosa-associated lymphoid tissue) lymphoma and mantle cell lymphoma in B cell lymphomas. We have analyzed the expression of bcl-2, cyclin D1, bcl-6 and p53 and correlated with the subtypes and histologic grade of primary gastrointestinal B cell lymphoma. Also we investigated the usefulness of immunophenotypic features in diagnosis of low grade B cell lymphoma. Materials and Methods : Twenty-two cases of primary gastrointestinal B cell lymphoma were recategorized in low grade MALT lymphoma, low/high grade MALT lymphoma, high grade MALT lymphoma, and diffuse large cell lymphoma according to the morphological findings. We investigated the expression of bcl-2, cyclin D1, bcl-6 and p53 by immunohistochemical method. Results : The bcl-2 protein expression was higher in the low grade MALT lymphoma than in the high grade lymphoma. The cyclin D1 protein expression was negative in all cases. The expression of bcl-6 and p53 protein was negative in all low grade MALT lymphoma. Conclusion : The results suggest that we can differentiate the low grade lymphoma from the high grade lymphoma by immunohistochemical staining for bcl-2, bcl-6, and p53 protien.

금강 수계 퇴적물 중 PCDD/Fs 및 Co-PCBs의 농도 분포와 발생원 해석

박종은(Jong Eun Park),김종국(Jong Guk Kim),안성윤(Sung Yun Ahn) 大韓環境工學會 2011 대한환경공학회지 Vol.33 No.12

본 연구에서는 금강 수계 퇴적물 중 PCDD/Fs 및 Co-PCBs의 농도 분포를 파악하고 발생원을 추정하기 위해 17개 지점에서 총 52개의 샘플을 채취하여 분석하였다. 금강 수계 퇴적물 표층에서 검출된 PCDD/Fs 및 Co-PCBs의 농도 수준은 각각 불검출~193.47(평균 84.52) pg/g-d.w.와 0.34~359.19(평균 114.65) pg/g-d.w.를 나타내었으며, TEQ 값은 각각 불검출~5.12(평균 0.88)pg I-TEQ/g-d.w와 불검출~0.58(평균 0.09) WHO₂005-TEQ pg/g-d.w.을 나타내었다. 유역별로는 하수종말처리장, 분뇨처리장, 매립장 등이 위치한 상류 지역에서 가장 높은 농도를 보였으며, 하천 하류 방향으로 갈수록 감소하는 경향을 보였다. 다이옥신이 검출된 지점에서는 고염소 화합물이 특징적으로 높은 패턴을 나타내었으며, Co-PCBs는 모든 퇴적물 시료의 이성체 패턴이 PCBs제품의 패턴과 유사한 경향을 보였다. Co-PCBs의 발생원 해석을 위해 통계 분석을 수행한 결과 상업적 PCBs 제품에 의한 영향이 큰 것으로 나타났다. To investigate concentration and sources of PCDD/Fs and Co-PCBs in sediments, total 52 sediment samples were measured at 17 sites in Geum River. As a result of analysis, total concentrations of PCDD/Fs and Co-PCBs were ranged from not detected (N.D.)~193.47 pg/g-d.w. (mean value: 84.52 pg/g-d.w) and 0.34~359.19 pg/g-d.w (mean value: 114.65 pg/g-d.w.) respectively. Also, toxic equivalent (TEQ) concentration of PCDD/Fs and Co-PCBs were ranged from N.D.~5.12 (mean value: 0.88) pg I-TEQ/ g-d.w and N.D.~0.58 (mean value: 0.09) WHO₂005-TEQ pg/g-d.w. respectively. The upstream indicate the highest concentration and as it goes to the downstream, it has tendency to decrease. Higher chlorinated compounds were dominant in most of site which detected PCDD/Fs. Occurrence of Co-PCBs isomer patterns appear similar to each sampling site was found. The isomer patterns showed similar between sediment and PCB products. The principal component analysis also showed that the samples contained the characteristics of PCBs products.

청주시 토양 중 Dioxin-like PCBs 농도 및 특성

박종은 ( Jong Eun Park ),김성준 ( Sung Joon Kim ),김종국 ( Jong Guk Kim ) 한국환경분석학회 2010 환경분석과 독성보건 Vol.13 No.2

To investigate concentration and characteristics of Dioxine-like PCBs in soil, Cheongju city, total 28 soil samples were collected. The total and TEQ concentration of 12 DL-PCBs ranged from 10.82 to 234.84 pg/g dry weight (mean conc. 65.07) and from 0.002 to 0.399 pg TEQ/g dry weight (mean conc. 0.062), respectively. These levels are lower or similar that of various soil which were conducted by ministry of environment of Korea from 2005 to 2007. The TEQ concentration as land use was high as follows; industrial area > green area > residential and commercial area > farm land. In terms of congener distribution, the congener profile suggested that DL-PCBs sources could be related to commercial products of PCB.

장결핵의 감별진단에 있어서 3 개월 추적 대장내시경 검사의 의의

연종은(Jong Eun Youn),박이병(Ie Byung Park),권소영(So Yung Kwon),김재선(Jae Seon Kim),변관수(Kwan Soo Byun),박영태(Young Tae Bak),김종극(Jong Guk Kim),이창홍(Chang Hong Lee),김진호(Jin Ho Kim) 대한내과학회 1996 대한내과학회지 Vol.50 No.2

N/A Objectives: A therapeutic trial with antituberculous drugs is reasonable if the clinical and colonoscopic features are compatible, in countries with a high prevalence of intestinal tuberculosis. But Crohn's disease, lymphoma and other infectious disease should be differentiated from the disease. There have been no established monitoring modalities to assess the efficacy of the therapeutic trial and to differentiate these diseases yet. Therefore, this study was done to assess the usefulness of follow-up colonoscopy at 3 months of antituberculous treatment as a monitoring modality. Methods: 42 patients with tentative diagnosis of intestinal tuberculosis which was made on the basis of clinical and colonoscopic findings were enrolled, The patients were divided into 2 groups according to the symptomatic improvement after the therapy : (A) 30 patients with improved symptom : (B) 12 patients with persistent symptom. Colonoscopy with biopsy was performed before and at 3 months of therapeutic trial. Standard four-drug antituberculous chemotherapy was done at least for 9 months and patients were followed for more than a year after completion of therapy. Final diagnoses were made on the basis of satisfactory outcome of therapeutic trial, operative findings and another follow-up colonoscopy. Results: 1) colonscopic findings at 3 months of therapy improved in 31 cases. 27 of them were finally diagnosed as tuberculosis, 1 was as ulcerative colitis and 3 were lost. Among 11 endoscopically unimproved cases, 7 were finally diagnosed as Crohn's disease, 2 were as tuberculosis and 2 were lost. 2) 27 of 29 patients with final diagnosis of intestinal tuberculosis improved colonoscopically at 3 months of therapy. 2 unimproved cases had a complicated disease(stenosis) or an atypical mycobacterial infection, respectively. 3) In group A, 1 was diagnosed as ulcerative colitis and in group H, 3 were diagnosed as tuberculosis finally. Conclusion: Follow-up colonoscopy at 3 months of antituberculous therapeutic trial in patients with tentative diagnosis of intestinal tuberculosis seems to be a valuable modality for monitoring the efficacy of therapy and differentiating intestinal tuberculosis from other diseases.

국내 주요 수계 표층 퇴적물 중 HCB와 DDTs의 농도분포 특성에 관한 연구

박종은(Jong Eun Park),이상천(Sang Chun Lee),홍종기(Jong Ki Hong),김종국(Jong Guk Kim) 大韓環境工學會 2012 대한환경공학회지 Vol.34 No.5

본 연구에서는 우리나라 대표적인 4대강 수계의 퇴적물을 대상으로 HCB와 DDTs 농도분포 특성에 대해 검토하였다. HCB 농도는 한강 0.41~3.80(평균 1.58) ng/g, 낙동강 0.08~6.09(평균 0.90) ng/g, 금강 0.02~0.97(평균 0.30) ng/g, 영산강 0.28~0.59(평균 0.42) ng/g, 섬진강 0.23~0.48(평균 0.32) ng/g으로 검출되었다. DDTs 농도는 한강 0.67~14.20(평균 4.76) ng/g, 낙동강 N.D.~10.36(평균 1.81) ng/g, 금강 N.D.~7.26(평균 1.87) ng/g, 영산강 N.D.~3.12(평균 1.08) ng/g 및 섬진강에서 0.02~2.04(평균 0.56) ng/g으로 검출되었다. 우리나라 하천 퇴적물 중 HCB와 DDTs 농도는 다른 나라의 강이나 하구언 퇴적물 농도와 비교해도 상대적으로 낮게 나타났다. 퇴적물 시료에서 검출된 농도를 미국해양기상연구소가 제시한 퇴적물권고기준치와 비교한 결과 HCB의 농도는 모두 ERL 값에 크게 미치지 않는 것으로 조사되었다. DDTs의 경우 46개 시료에서 ERL(1.58 ng/g)을 초과하는 농도를 나타내어 현재 퇴적물 중 DDTs 농도가 저서생물에 위해한 영향을 줄 것으로 예상되는 수준은 아니나 지속적인 관찰이 요구되는 것으로 판단되었다. Hexachlorobenzene (HCB) and Dichloro-Diphenyl-Trichloroethane (DDT) were determined in surface sediments collected from main rivers of Korea. Concentration of HCB in sediments ranged from 0.41 to 3.82 (average 1.58) ng/g, 0.08 to 6.09 (average 0.90) ng/g, 0.02 to 0.97 (average 0.30) ng/g, 0.28 to 0.59 (average 0.42) ng/g and 0.23 to 0.48 (average 0.32) ng/g in Han river, Nakdong river, Geum river, Yeongsan and Seomjin river respectively. The DDTs concentration was ranged from 0.67 to 14.20 (average 4.76) ng/g, N.D. to 10.36 (average 1.81) ng/g, N.D. to 7.26 (average 1.87) ng/g, N.D. to 3.12 (average 1.08) ng/g and 0.02 to 2.04 (average 0.56) ng/g in Han river, Nakdong river, Geum river, Yeongsan and Seomjin river respectively. In comparison with the concentration of HCB and DDTs in other studies, the values in sediments of this study were lower than those of other countries. Comparison with that Sediment quality guideline (SQG) of National Oceanic and Atmospheric Administration (NOAA), the HCB levels of this study were very lower than Effect Range Low (ERL) value. In the case of DDTs, the concentrations of 46 points were higher than ERL (1.58ng/g). It have not harmful effect on ecosystem of the sediment, however ongoing monitoring of sediments is deemed necessary.

급성관절염 환자에서 진단된 성인형 주기성 호중구감소증 1예

이명진 ( Myung Jin Lee ),유종진 ( Jong Jin Yoo ),강은하 ( Eun Ha Kang ),이상국 ( Sang Guk Lee ),신기철 ( Ki Chul Shin ),이은영 ( Eun Young Lee ),이은봉 ( Eun Bong Lee ),송영욱 ( Yeong Wook Song ),이윤종 ( Yun Jong Lee ) 대한류마티스학회 2013 대한류마티스학회지 Vol.20 No.1

저자들은 발열과 다발성 관절염으로 내원한 환자에서 성인형 주기성 호중구감소증을 진단하고 규칙적인 G-CSF 투약을 통해 치료한 증례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Cyclic neutropenia (CN) is a rare disorder characterized by repetitive episodes of neutropenia and is generally associated with fever, oral mucosal ulcers, and bacterial infections in the neutropenic episodes. It usually manifests initially in infancy or childhood as an autosomal dominant or sporadic condition; however, adult-onset CN may have an autoimmune etiology. Here, we report the first case of a 22-year old man with CN in Korea. He developed acute arthralgia and fever 4 weeks after an episode of lower gastrointestinal symptoms. Serial blood cell counts showed recurrent neutropenia at 3 week intervals. Further, laboratory examination for neutropenia, including neutrophil elastase gene sequencing, did not reveal any abnormality. His arthritis and periarthritis fluctuated during his course. Under the diagnosis of CN, he received regular G-CSF therapy with partial improvement.

Cultivation of Micractinium inermum in Liquid Fertilizer Mixed Medium using Flat-panel PBR System

Eun Kyung KIM,Gang-Guk CHOI,Jong-hee KWON,Ji-Won YANG 한국생물공학회 2013 한국생물공학회 학술대회 Vol.2013 No.10

[Brief Communication] Plagiorchis muris infection in Apodemus agrarius from northern Gyeonggi-do (Province) near the demilitarized zone

Jong-Yil CHAI,Jae-Hwan PARK,Sang-Mee GUK,Jae-Lip KIM,Hyo-Jin KIM,Won-Hee KIM,Eun-Hee SHIN,Terry A. KLEIN,Heung-Chul KIM,Sung-Tae CHONG,Jin-Won SONG,Luck-Ju BAEK 대한기생충학열대의학회 2007 The Korean Journal of Parasitology Vol.45 No.2