Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

FDM에서 Build Orientation이 쾌속조형물의 표면에 미치는 영향

전재억,서진호,권광진,권혁준,정진서,하만경 한국공작기계학회 2002 한국공작기계학회 춘계학술대회논문집 Vol.2002 No.-

Fused deposition (FD) modeling by Stratasys Inc., is one of the material deposition subfamilies of solid freeform fabrication (SFF) technologies. In this process, build material in the form of a flexible filament, is heated to a semi-liquid state and extruded from a controlled deposition head onto a fixtureless table in a temperature controlled environment. The position of nozzle is computer controlled relative to the base, which allows geometric complex models to be made to precise dimensions. FDM provide what the part was directly tested by the worker. It provide believable data. This Study is identify to surface roughness by build orientation adjustment. So, the paper is the study on surface roughness by build orientation at FDM.

제주도 지하수 질산염 농도의 시·공간적 변화 특성: 장기(1993-2015) 모니터링 자료의 평가

김호림(Ho-Rim Kim),오준섭(Junseop Oh),도현권(Hyun-Kwon Do),이경진(Kyung-Jin Lee),현익현(Ik-Hyun Hyun),오상실(Sang-Sil Oh),감상규(Sang-Kyu Kam),윤성택(Seong-Taek Yun) 대한자원환경지질학회 2018 자원환경지질 Vol.51 No.1

1993년부터 2015년까지 관측된 제주도 지하수 장기모니터링 관측정(N = 4,835)에서 수집된 지하수 수질자료(N = 21,568)를 기반으로 질산성질소의 시공간적 변동 특성을 평가하였다. 제주도 지하수의 질산성질소 농도의 중앙값은 2.5 mg/L로서 다른 국가나 대륙의 조사 결과에 비해 다소 높거나 유사한 것으로 나타났다. 또한 지하수 용도, 행정구역 및 고도 별로 유의한 차이를 보였다. 특히, 산간 지역에 비해 저지대 해안가에 위치한 농업 및 주거지역에서 농도가 높음을 확인하였다. Mann-Kendall 및 Sen’s slope 분석을 활용한 질산성질소 농도의 추세 분석 결과, 하류 저지 대에 비해 중산간지역에서의 질산성질소 농도 증가 경향이 뚜렷하였다. 제주도 내 토지 피복의 시계열 변화 특성과 결부 지어 보면, 중산간지역의 오염 증가 추세는 농업지역의 확장 등 인위적 활동 증가에 기인한 결과로 판단된다. 반면,기지정된 지하수자원특별관리구역에서는 전반적으로 질산성질소 농도의 감소 경향이 나타났는데, 이는 지하수 관리 측면에서 수질관리를 위한 적극적인 정책이 유효함을 시사한다. 본 연구에서는 제주도 지하수의 질산성질소 오염관리를 위한 적정 방안을 제안한다. The spatio-temporal variations of nitrate concentrations in groundwater of Jeju Island were evaluated by an analysis of time series groundwater quality data (N = 21,568) that were collected from regional groundwater monitoring (number of wells = 4,835) for up to 20 years between 1993 and 2015. The median concentration of NO 3 -N is 2.5 mg/L, which is slightly higher than those reported from regional surveys in other countries. Nitrate concentrations of groundwater in wells tend to significantly vary according to different water usage (of the well), administrative districts, and topographic elevations: nitrate level is higher in low-lying agricultural and residential areas than those in high mountainous areas. The Mann-Kendall trend test and Sen’s slope analysis show that nitrate concentration in mid-mountainous areas tends to increase, possibly due to the expansion of agricultural areas toward highland. On the other hand, nitrate concentrations in the Specially Designated Groundwater Quality Protection Zones show the temporally decreasing trend, which implies the efficiency of groundwater management actions in Jeju. Proper measures for sustainable groundwater quality management are suggested in this study.

체중감량이 유도선수의 Renin, 면역기능 및 유·무산소 운동능력에 미치는 영향

권영우,전승훈,김진해,민경선,민진아 한국스포츠리서치 2001 한국 스포츠 리서치 Vol.12 No.4

The purpose of this study was to investigate the effect of short terms weight reduction period on body composition and body fluid. Seven Judo athletes (21±1.21 yrs) were matched and randomly placed. Body weight reduction period consists of 7 day. Each groups reduced body weight by 3%(2th day from pre weight reduction), 5%(4th day from pre weight reduction, 7%(7th day) and investigated the recovery period on 3 hour after breakfast, lunch, and 1day, 2day after 7% body reduction. Weight reduction reduced weight during weight reduction period then returned to baseline during recovery(p<.05) and decreased LBM, electrolyte, and immune function and then increased in recovery period to baseline. In conclusion, weight reduction of Judo athletes caused the change biological variable and performance and STWR may detrimental effects body' homeostasis.

타액선 악성 종양의 임상적 연구

권혁진,김경욱 단국대학교 치의학연구소 1996 논문집 Vol.8 No.1

Malignant tumors of the salivary glands account for a relatively small number(4%) of head and neck tumors, but generate considerable interest because of their remarkable variation in clinical presentation, histologic appearance, and behaviors. Successful treatment of these lesions is a major challenge because of the disposition of these tumors to recur locally as well al distantly. In my study, 46 cases treated from 1982 to 1995 at Korea cancer center hospital are analyzed retrospectively & clinically. The obtained results were as followings. 1. The mean age of patients was 52.7 years old and male & female ratio was 6:4. 2. The common symptoms were mass(45.6%), pain(19.5%), ulcer(13%) and the duration of chief complaints almost was within 6 months. 3. According to histopathology, adenoid cystic carcinoma was most common (43%), and according to the criteria of the AJCC on staging, stages were as followings: stage 1 10.8%, stage 2 23.9%, stage 3 26%, stage 4 39.1%. 4. In treatment modalities, modified triple modalities(chemotherapy + radiation therapy + surgery) was most common(28%), surgery alone was 21.7%, and surgery combined radiation therapy was 19.5%. 5. Among all 46 patients, there were 17%(37%) recurrences. Locoregional recurrence alone occured in 10 patients, distant metastasis alone in 11 patients, and locoregional recurrence with distant metastasis in 2 patients. 6. Overall survival rates of entire group by Kaplan-Meier method was 50.1% at 5 years. 7. The 5 year survival rate was 77.1% in early staged patients, in contrast to 35.3% in advanced patients(P<0.05). 8. The 5 year survival rate for patients without cervical lymph node metastasis was 56.6%, in contrast to 36.9% for patients presented with nodal disease initially. 9. In 5 year survival rates according to histopathology 46.6% in mucoepidermoid carcinoma, 49.2% in adenoid cystic carcinoma, 40% in adenocarcinoma & malignant mixed tumor & malignant myoepithelioma, 100% in terminal duct carcinoma were seen. 10. In 5 year survival rates according to various modalities of treatment, 20% in untreated patients and 83.8% in surgery alone, 68.5% in surgery combined radiation therapy, 46.6% in modified triple modalities (chemotherapy + radiation therapy + surgery) were seen(P<0.01).

中等學校 科學敎育의 實態에 관한 調査 硏究

權寧駿,成辰慶 공주대학교 사범대학 과학교육연구소 1992 과학교육연구 Vol.23 No.1

The purpose of this study is to provide the science teachers with fundamental sources to ameliorate the condition of science education. This study was carried out by a following process-to approach and identify the problems of science education-the statistical comparison and analysis of the materials from the surveying questions concerning the current issues which the science teachers of secondary school have been suffering. Teh issues are surveyed by the classified questions as below: of curriculum, especially experimentation, educational environment, students and teachers. Under the targets of the random sampling investigation was 430 secondary schools, which were located in the direct control city of Taejon, Chungchong nam-do and Chungchong puk-do. Simultaneouely posted on the 16-th day of september, 1991, 319 of the inquiry papers, which were sent back thanksfully. The rate of the replies amount to 74.2 percentage. The remarked materials were compared and analyzed with those of 1974 and 1983. All of the data as below are described by means of the numerical value of percentage and average. The out-put results are represented as below: 1. Until recently, science curricular goals are illustrated to be so desirable, while little difference was made in the contents of science education between the two decaded. The learning methods of students have been greatly altered from the learning by testing to the learning by discovery. Nevertheless the teaching methods of teachers have been clung to the cramming system of education so far. Moreover, school hours in a week are explained to be quite less than demanded. What is needed, most of all, is to fabricate the contents of curriculum, to add school hours in a week, to give priority to the environment education and the environment science, and to expand the special intelligence education in the field of science education. 2. In the science curriculum, the heterogeneous groups are managed through the massed learning and the aspects of experimentation are transfered to the group experiment. On the other hand, the system of learning by inquiry is not well-operated as usual. Hence, it is so much requested theat we should reform the entrance examination system and normalize the science curriculum. 3. Education facilitaies are, if anything, poorly-equipped. It is needless to say that eductional enviorment should be well-equipped with comfortable classroom, school, and convenient condition for experimentation. 4. There is a tendency that most of the students think a science subject as one of the most difficult. Almost students hopes their classes will be carried out by experimentation. Accordingly, the contents of science education should be understandably trans-figured for students to be attracted. 5. The school career of science teacher truned out to be growing higher. Although having more chances to join the research study courses, many of the teachers think they have fewer than hope. Many of them are not satisfied with their teaching professions. Burdensome tasks are of course ascribed to their discontent. Consequently, the problems how we can help the science teachers to uphold the professional faculty, necessary to the practical teaching activities and the accomodation of future society, should be settled as below; to establish the advanced research study cources, to decrease the burdensome tasks of science teachers, and to build up the society so that the teachers may be cordially respected, and to accomplish the institutionalized system so that the teachers may receive everyone`s hospitality.

사이버 가정학습체제 구축 모형 개발

권성호,최인호,심현애,이승진,김경현,장상현 한국교육정보미디어학회 2003 교육정보미디어연구 Vol.9 No.4

본 연구는 초·중등 사이버 가정학습체제의 구축 모형 개발을 목적으로 한다. 이를 위해 첫째, 사교육비 관련 현황 및 우리나라의 사회·현실적 요구를 분석하고, 둘째, 사이버 공간에서의 학습을 지원하는 학습이론을 탐색하여 얻은 시사점을 바탕으로 사이버 가정학습체제 개발 원리를 도출하였으며, 셋째, 학습이론적 적용의 틀을 기반으로 사이버 가정학습체제 구축 모형을 개발하고, 각 모형에 대한 세부적인 설명과 시행 시 고려해야 할 사항을 제시하였다. 사이버 가정학습체제 구축 모형은 체제 구성요소와 각 요소들 간의 관계를 도식화한 체제 구성도를 바탕으로 사이버 가정학습체제를 운영하는 활동 주체들의 역할을 정의한 운영 모형, 사이버 가정학습체제 내에서의 학습 유형과 흐름을 보여주는 학습 모형, 사이버 가정학습체제 내 학습 콘텐츠의 구성 기준을 제시하는 콘텐츠 모형 그리고 사이버 가정학습체제 내에서의 학습에 대한 학업성취도 평가와 체제 평가를 위한 평가 모형으로 구성되었다. 각 유형별 모형은 필요에 따라 하위 모형으로 분류되어 총 17개의 사이버 가정학습체제 모형을 제시하였다. 또한 사이버 가정학습체제가 사교육비 절감과 공교육 지원이라는 궁극적인 목적을 달성하기 위해 고려되어야 할 시사점으로 환경자원, 학습자원, 인적자원, 홍보 마케팅 등의 네 가지 측면으로 나누어 제시하였다. The study is purposed to develop a set of construction models for Cyber Home Study System(CHSS) to support the students' self study through internet at the level of elementary, middle and high school students. This is intended to reduce the over spending of the private educational payment, causing the social conflict and antagonism in Korea, and finally to support the public education. The study problems are as follows: 1. Analysis on the social and authentic needs for reducing the private educational payment which causes the social conflict and antagonism in Korea. 2. Setting up the principles to develop the construction model for CHSS to apply the learning theories in support of the learning in the cyber space. 3. Development of a set of construction models for development and construction for CHSS, based on the theoretical developmental principles. The construction model for CHSS developed in this study contains a systemic diagram of the whole CHSS, and 4 major models: a model for management, the model for learning process, the model for content design, and the model for evaluation. The systemic diagram for CHSS demonstrates various components, which consist of the whole system, and the relationship among the components. The model of management identifies the role of each organization by which the CHSS is operated. The Learning process model is classified with 9 sub-models converging on the three factors. These factors are the learner subject, the existence of tutor support, and the relationship to the local school. The models for content design proposes the guideline for design and production of learning content. The evaluation model is composed of three sub models: evaluation for individual learning achievement, evaluation for learning achievement of the tutor supported calss members, and the system evaluation. Finally, 4 kinds of suggestions is suggested to accomplish the purpose of CHSS in terms of environmental resources, learning resources, man power, and advertising and marketing strategies.

체세포복제배아 줄기세포의 최근 연구 동향과 관련 윤리지침

권복규,안경진 이화여자대학교 생명의료법연구소 2007 생명윤리정책연구 Vol.1 No.1

NT-hESC research has been receiving much attention, because it holds an immense potential to cure incurable diseases. However, many ethical debates are waged concerning NT-hESC. The debates center around the issue of destroying embryos and acquiring egg donations, because in order to get stem cell lines, the artificial making and destroying of embryos are necessary. Many stem cell scientists around the world continue NT-hESC research within the boundaries of their country’s laws, policies and ethical guidelines. In this article, I first examine the recent NT-hESC research and the naturally accompanying ethical debates regarding the moral status of embryos. Differences in the interpretation and understanding of the moral status of embryos stemming from various cultures, religions and social structures enhance the debate as current scientific technology requires the artificial creation and destruction of embryos in order to get stem cell lines. Second, I examine the ethical issues related to the acquisition of research materials such as egg donation. I explore the National Academy of Science’s “Guidelines for Human Embryonic Stem Cell Research” which contains detailed articles related to policy and ethical considerations. Based on this, I propose that Korea extend the basic ethical and practical regulations in determining ethical and policy guidelines for NT-hESC research.

음향방출을 이용한 저어널 베어링의 조기파손감지(Ⅱ) : Part Ⅱ : Intervention of Foreign Particles in Lubrication 윤활유 이물질 혼입의 영향 및 감시

권오양,윤동진,김경웅,정민화 韓國非破壞檢査學會 1994 한국비파괴검사학회지 Vol.14 No.2

일반적으로 회전기기의 저어널 베어링 부분은 윤활유 공급의 부족이나 윤활층에 이물질이 혼입되면 시스템의 고장이나 가동중단 등의 원인이 되기도 한다. 따라서 베어링 손상에 기인하는 사고와 관련된 안전운전 문제와 유지비용의 절감을 위해 여러가지 파괴 및 비파괴시험법들이 사용되어 왔다. 본 연구에서는 저어널 베어링에서 가장 발생하기 쉬운 윤활층에의 이물질이 혼입에 의해 야기되는 베어링 파손의 조기검출을 위해 음향방출 기술을 적용하였으며, 전보의 연구에 이어 좀더 정량적이고 체계적인 실험을 수행하였다. 실험실용으로 직접 제작한 모의베어링 시스템을 이용하여 여러 형태의 인위적인 이물질 흔입 상태를 만들어 실험하였으며 베어링 손상 및 결함 형태의 해석을 위해 AE rms level, 파형분석, AE 변수 등의 여러 파라메터를 사용하여 분석 고찰하였다. 그 결과 AE rms level의 변화가 이물질 흔입의 영향에 민감함을 보여주었으며 AE변수등 다른 정보들로부터 손상 원일별로 신호형태를 확인할 수 있었다. Journal bearings in the rotating machineries are vulnerable to the contamination or the insufficient supply of lubricating oil, which is likely to be the cause of unexpected shutdown or malfunction of these systems. Various destructive and nondestructive testing methods had been used for the reduction of maintenance cost and the operational safety problems due to the accidents related to bearing damages. In this experimental approach, acoustic emission monitoring in employed to the detection of incipient failure caused by intervention of foreign particles most probable in the journal bearing systems. Experimental schedules for the intervention of foreign particles was composed to be more quantitative and systematic than last study in consideration of minimum oil film thickness and particle size . The experiment was conducted under such designed conditions as inserting alumina particles to the lubrication layer in the simulated journal bearing system. Several parameters such as AE rms level, waveform, AE energy distribution and other AE event parameter are used for analysis and characterization of damage source. The results showed that the history of damage was well correlated with the changes of AE rms level and the types of damage source signal can be verified using other informations such an waveform, distribution of AE parameter etc.

FDM 장치에서 주사량과 시제품의 경사가 표면거칠기에 미치는 영향

권광진,전재억,정진서,김광희,하만경 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

Competitive power is rapidly manufacture product required consumers. Therefore, modern industry have changed from small item many production to many item small production. rapid production is necessary thing at the product development. Thus, rapid prototyping is appeared. If the graphic model was made by CAD, the production can be made in short term. That provide what the part was directly tested by the worker. It provide believable data. This study is experiment on surface roughness of part at FDM