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삼환계 항우울제(Dothiepin)가 우울증 환자의 수면에 미치는 영향 : 수면 다원 검사에 의한 연구 A Polysomnographic Study
홍승철,한진희,이성필,방승규 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.4
우울증환자군에서 삼환계 항우울제인 dothiepin 투여 후의 수면이 변화양성을 알아보며, 치료 후의 호전정도를 예측할 수 있는 치료 전과 치료 후의 수면변인을 조사하기 위하여 본 연구를 시행하였다. 수면상태는 수면다원검사를 실시하여 평가하였고, 우울증상의 심각도와 임상양상의 정도는 Hamilton 우울척도를 이용하여 평가하였다. 연구대상은 최소 14일 이상 약물을 투여 받지 않은 우울증 환자 16명(남자 7명, 여자 9명, 연령분포 23∼64세)으로 구성되었다. 수면다원검사는 약물을 투여하기 전과 약물투여 후 1주와 3주에 시행하여 다음과 같은 결과를 얻었다. 1) 항우울제 투여 1주와 3주 후 수면 연속성은 기준수면에 비하여 총수면 시간이 유의하게 증가하였고 수면잠재기는 단축되었으며, 수면도중 각성시간은 감소하였고, 수면효율은 증가되었다. 2) 항우울제 투여 1주와 3주 후 수면구조에서는 기준수면에 비하여 2단계 수면이 유의하게 증가하였다. 3) 항우울제 투여 1주와 3주 후 급속안구운동 수면은 기준수면에 비하여 유의하게 감소하였고, 급속안구운동 수면잠재기는 증가하였다. 4) 항우울제 투여 1주 후와 3주 후의 수면을 비교시 수면잠재기가 3주에 유의하게 감소된 외에는 수면 변인의 차이를 보이지 않았다. 5) 우울증상의 평가를 위하여 약물투여 1주 후와 3주 후에 시행한 Hamilton 우울척도 점수는 약물투여 전에 비하여 줄어들어 우울증상이 호전됨을 나타내었다. 6) 항우울제 투여 3주 후 치료반응이 좋았던 군의 수면특징은 기준수면에서 수면도중 각성시간이 증가된 경향을 나타내었고, 약물투여 1주 후의 수면에서는 4단계 수면의 양이 유의하게 감소되었다. 이상의 결과를 볼 때, 우울증환자에서 삼환계 항우울제인 dothiepin 투여 후의 수면변화는 치료초기에 나타났다. 기준수면에서 각성기간의 증가와 1주 후의 수면에서 4단계 수면의 감소는 약물치료에 좋은 반응과 관련된 수면변인으로 생각되었다. Objectives : This study was designed to investigate 1) sleep changes after antidepressant (dothiepin) treatment, and 2) sleep variables which seem to be associated with clinical response in the depressed patients. Methods : The subjects consisted of 16 patients who fullfilled the criteria for major depression by the Diagnostic and statistical Manual, (4th edition). Their sleep was recorded using polysomnography at the baseline and after one week and three weeks of dothiepin treatment. All subjects were further interviewed using Hamilton Rating Scale for Depression (HRSD) to rate the severity of their depression. High response to the drug was defined as a reduction of more than 50% of the HRSD score. Results : The results were as follows : 1) Depressed patients after dothiepin treatment showed more total sleep time(p=0.019), shorter sleep latency(p=0.05), less awake time(p=0.033), more sleep efficiency(p=0.018), more stage 2 sleep(p=0.002), less REM time(p=0.000), and longer REM sleep latency(p=0.004) than before treatment. 2) There were no differences in sleep variables between those who received 1 week and 3 weeks of dothiepin treatment except for the shortening of sleep latency after 3 weeks(p<0.05). 3) Depressive symptom scores on HRSD were reduced after 1 week and 3 weeks of dothiepin treatment as compared with the baseline. 4) High responders showed a tendency of increased wake time(p=0.054), while their stage 4 sleep decreased after 1 week of dothiepin treatment as compared with the low responders(p=0.0136). Conclusion : These results suggest that sleep of the depressed patients after dothiepin treatment tends to be normalized and sleep changes seem to appear early in the treatment phase. In addition, clinical response might be associated with greater wake time at the baseline and lesser stage 4 sleep after 1 week of dothiepin treatment.
Bacillus sp. FF-7에 의한 항산화물질 생산조건과 항산화 활성
차재영,김효정,전방실,박진철,옥민,조영수 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.3
발효식품 시료로부터 분리된 세균을 DPPH(α,α¹-diphenyl-β-picrylhydrazyl) 전자공여능으로 항산화 활성을 측정하여 가장 활성이 강한 균주를 선별하여 형태학적, 생화학적, 생리학적 특성 및 16S rRNA 염기서열을 조사한 결과 Bacillus sp.으로 판명되어 FF-7로 명명하였다. DPPH 전자공여능법에 의한 Bacillus sp. FF-7이 생산하는 항산화물질의 최적 생산 배지조건은 탄소원 2% galactose와 질소원 1% tryptone 첨가였다. Bacillus sp. FF-7에 의해 생성된 항산화 물질의 활성을 DPPH 전자공여능, 흰쥐 각 조직 microsomal 실험계 및 linoleic acid 과 산화지질 실험계에서 malondialdehyde를 thiobarbituric acid(TBA)방법으로 측정하였다. 흰쥐 각 조직 microsomal 실험계에서 지질과산화에 대한 항산화 효과는 뇌(97.50%)>심장(79.95%)>신장(77.84%)>비장(77.47%)>고환(69.96%)>간장(62.45%) 순이였다. Linoleic acid의 과산화지질률 TBA법으로 측정한 결과 반응 4일째까지 억제 효과가 강하게 나타났으며, 동시에 대조구로 사용한 0.05% BHT 첨가구에서도 실험종료시까지 항산화 활성이 강하게 나타났다. The antioxidative activity of antioxidative substances produced from several bacterial strains isolated from fermented foods were tested by DPPH (α,α'-diphenyl-β-picrylhydrazyl) free radical scavenging activity. One of the strains showing the highest antioxidative activity was identified as Bacillus sp. based on the morphological, biochemical, physiological characteristics, and 165 rRNA sequence, and named FF-7. The molt optimal medium condition for the production of antioxidative substance from Bacillus sp. FF-7 was 2% galactose as carbon source and 1% tryptone as nitrogen source. The antioxidative substance produced from FF-7 in these cultural medium was also tested by in vitro experimental models, the peroxidation of linoleic acid and the peroxidation of rat tissues microsomes by using thiobarbituric acid (TBA) for assay of free rnalondialdehyde production. The antioxidative avtivity against lipid peroxidation of rat tissues microsomes was shown in the following order; brain 97.50% > heart 79.95% > kidney 77.84% > spleen 77.47% > testis 69.96% > liver 62.45%. The antioxidative substance produced from FF-7 on linoleic acid peroxidation by TBA method was effectively inhibited during four days, and 0.05% BHT(butylated hydroxytoluene) used comparative control was also effectively inhibited. Results showed that the highest antioxidative activity by DPPH method of antioxidative substance produced from Bacillus sp. FF-7 was obtained by supplementing 2% galactose as carbon source and 1% tryptone as nitrogen source in cultured medium, this substance effectively inhibited the formation of TBARS in brain microsome in vitro system and in linoleic acid peroxidation.
Melanogenesis inhibitory pregnane glycosides from <i>Cynanchum atratum</i>
Jin, Qinghao,Han, Xiang Hua,Yun, Cheong-Yong,Lee, Chul,Lee, Jin Woo,Lee, Dongho,Lee, Mi Kyeong,Jung, Sang-Hun,Hong, Jin Tae,Kim, Youngsoo,Hwang, Bang Yeon Elsevier 2018 Bioorganic & medicinal chemistry letters Vol.28 No.7
<P><B>Abstract</B></P> <P>Bioassay-guided fractionation of the methanolic extract from the roots of <I>Cynanchum atratum</I> has resulted in the isolation of three new pregnane glycosides (<B>1</B>–<B>3</B>) along with four known compounds (<B>4</B>–<B>7</B>). Their structures were identified by analysis of the spectroscopic data including extensive 2D NMR. All of the isolates were evaluated for their potential to inhibit the melanin production in α-melanocyte stimulating hormone (α-MSH)-activated B16 melanoma cells. Of these, compounds <B>4</B>–<B>7</B> dose-dependently inhibited the melanin production with the IC<SUB>50</SUB> values ranging from 4 μM to 33 μM.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Two New <i>C</i>‐<i>seco</i> Limonoids from the Fruit of <i>Melia azedarach</i>
Jin, Qinghao,Lee, Chul,Woo ,Lee, Jin,Yeon ,Choi, Jeong,Tae ,Hong, Jin,Kim, Youngsoo,Kyeong ,Lee, Mi,Yeon ,Hwang, Bang WILEY‐VCH Verlag 2014 Helvetica chimica acta Vol.97 No.8
<P><B>Abstract</B></P><P>A phytochemical investigation of the fruits of <I>Melia azedarach</I> led to the isolation of two new C‐<I>seco</I>‐nimbolinin‐type limonoids, 3<I>α</I>‐acetoxy‐1<I>α</I>,7<I>α</I>‐dihydroxy‐12<I>α</I>‐methoxynimbolinin (<B>1</B>) and 3<I>α</I>‐acetoxy‐1<I>α</I>,12<I>α</I>‐dihydroxy‐7<I>α</I>‐(2‐methylprop‐2‐enoyl)nimbolinin (<B>2</B>), together with eleven known compounds, <B>3</B>–<B>13</B>. Their structures were elucidated on the basis of extensive spectroscopic analysis, including <SUP>1</SUP>H‐ and <SUP>13</SUP>C‐NMR, HMQC, HMBC, NOESY, and HR‐FAB‐MS.</P>
( Chul Hwan Bang ),( Yun-mi Jeong ),( Hae Eun Park ),( Jin Woo Park ),( Ga Hee Jeong ),( Min Ji Park ),( Yunjae Lee ),( Songwan Jin ),( Ji Hyun Lee ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1
Background: Although advances have been made in in vitro technologies of culturing cells, there is limitation that microenvironment in which cells are cultured is not same as that in human skin, making it difficult to predict therapeutic response. Using 3D bioprinting in in vitro melanoma tissue culture would provide environments more similar to the physiologic conditions, realizing personalized medicine. Objectives: We investigated whether melanoma acquired from patient-derived xenograft model (PDX) is well maintained and proliferated when tissue culture is performed in 3D bioprinited skin. Methods: The melanoma acquired from PDX was sectioned into 0.2-cm diameter and 0.1-cm height. They were cultured on 3D bioprinted collagen, dermis, and full-thickness skin, and the size change and cell viability of melanoma tissues were measured. Immunohistostaining and western blotting for cell markers of melanoma were performed. Results: The novel tissue culture model was well maintained until the 21st day. There was no significant change in diameter or height and cell viability. New melanoma nests proliferation was observed both grossly and microscopically at the 21st day. The characteristic findings of melanoma were well observed with immunohistostaining and western blotting. These results were best seen in the melanoma tissue culture with 3D bioprinted dermis group. Conclusion: Melanoma tissue culture with 3D bioprinted skin may be a novel method for melanoma culture and in vitro model of melanoma.
Jin, Qinghao,Lee, Chul,Lee, Jin Woo,Yeon, Eung Tae,Lee, Dongho,Han, Sang Bae,Hong, Jin Tae,Kim, Youngsoo,Lee, Mi Kyeong,Hwang, Bang Yeon American Chemical Society and American Society of 2014 Journal of natural products Vol.77 No.7
<P>Two new 2-phenoxychromones <B>1</B> and <B>2</B> and two prenylflavonoids <B>3</B> and <B>4</B> along with 12 known compounds (<B>5–16</B>) were isolated from the CH<SUB>2</SUB>Cl<SUB>2</SUB>-soluble fraction of a methanol extract of <I>Epimedium koreanum</I>. Compounds <B>1</B>, <B>4</B>, <B>6</B>, <B>7</B>, <B>9</B>, <B>10</B>, <B>12</B>, and <B>15</B> exhibit inhibitory effects on nitric oxide production with IC<SUB>50</SUB> values ranging from 16.8 to 49.3 μM. Compounds <B>1</B>, <B>4</B>, <B>7</B>, and <B>12</B> also showed inhibitory effects on interleukin-1β production with IC<SUB>50</SUB> values ranging from 8.6 to 38.9 μM in RAW 264.7 macrophages.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2014/jnprdf.2014.77.issue-7/np400831p/production/images/medium/np-2013-00831p_0004.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np400831p'>ACS Electronic Supporting Info</A></P>
( Na Jin Kang ),( Dong Hwan Koo ),( Gyeoung Jin Kang ),( Sang Chul Han ),( Bang Won Lee ),( Young Sang Koh ),( Jin Won Hyun ),( Nam Ho Lee ),( Mi Hee Ko ),( Hee Kyoung Kang ),( Eun Sook Yoo ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.3
Macrophage-derived chemokine, C-C motif chemokine 22 (MDC/CCL22), is one of the inflammatory chemokines that controls the movement of monocytes, monocyte-derived dendritic cells, and natural killer cells. Serum and skin MDC/CCL22 levels are elevated in atopic dermatitis, which suggests that the chemokines produced from keratinocytes are responsible for attracting inflammatory lymphocytes to the skin. A major signaling pathway in the interferon-γ (IFN-γ)-stimulated inflammation response involves the signal transducers and activators of transcription 1 (STAT1). In the present study, we investigated the anti-inflammatory effect of dieckol and its possible action mechanisms in the category of skin inflammation including atopic dermatitis. Dieckol inhibited MDC/CCL22 production induced by IFN-γ (10 ng/mL) in a dose dependent manner. Dieckol (5 and 10 μM) suppressed the phosphorylation and the nuclear translocation of STAT1. These results suggest that dieckol exhibits anti-inflammatory effect via the down-regulation of STAT1 activation.