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웹 서비스 기반의 지방자치단체 민원서비스 통합방안에 관한 연구
강임철,김용일 호남대학교 2004 호남대학교 학술논문집 Vol.25 No.2
As information technology is prevailing, the integration of business processes has been expanded from private to public sector. Korean government introduced e-government and began to provide civil services electronically. However, current system is neither efficient nor convenient enough because the system is not supporting one-stop portal service. We suggest a model of the integrated electronic civil service using web service technology that can offer service integration under the distributed environment, also implement a prototype system for managing application to display placards.
( So Ri Kim ),( Yong Chul Lee ),( Dong Im Kim ),( Yang Keun Rhee ),( Heung Bum Lee ),( Seoung Ju Park ),( Chi Ryang Chung ),( Seung Yong Park ),( Mi Ran Kang ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
Oxidative stress is well known to be implicated in the development of asthma. The mitochondrial respiratory chain is a major site of intracellular reactive oxygen species (ROS) generation and, at the same time, an important target for the damaging effects of ROS. Mito-Tempo is a specific mitochondrial ROS inhibitor and it is known to be associated with opening of mi-tochondrial permeability transition pore and inhibition of cell necroptosis or apoptosis. However, there is little information on the protective effects of Mito-Tempo on the inflammatory airway disorders including bronchial asthma and its acute exacerbation. We investigate the effects of Mito-tempo on the allergic airway inflammation and hyperresponsiveness using the mice sensitized with OVA and LPS and then challenged with OVA (OVALPS-OVA mice). The OVALPS-OVA mice showed the typical features of neutrophilic asthma; increased airway inflammatory cells, the pathologic changes, the increased levels of Th2 cytokines in lungs of OVALPS-OVA mice, increased mitochondrial ROS generation, and increased bronchial hyperresponsiveness. Interestingly, we found that in OVALPS-OVA mice, Mito-Tempo, a novel mitochondrial targeting agent significantly reduced the increases in inflammatory cytokines, mitochondrial ROS generation, airway inflammation, and bron-chial hyperresponsiveness. These findings indicate that mitochondrial dysfunction including oxidative damage may be im-plicated in the pathogenesis of bronchial asthma and provide the therapeutic potential of a mitochondrial targeting agent, Mito-Tempo, for bronchial asthma.
The Relationship between Mitochondrial ROS and ER Stress in Allergic Airway Diseases
( So Ri Kim ),( Yong Chul Lee ),( Dong Im Kim ),( Mi Ran Kang ),( Yang Keun Rhee ),( Heung Bum Lee ),( Seoung Ju Park ),( Chi Ryang Chung ),( Seung Yong Park ),( Hee Jung Kim ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
Mitochondria and the Nox family of NADPH oxidase are the two major sources of reactive oxygen species (ROS) that are induced by external stimuli, and the mitochondria respiratory chain is considered as an important site of ROS production within most cells. Recent evidence has demonstrated that various biological stimuli increase accumulation of unfolded or mis-folded proteins in ER lumen, which is referred to as "ER stress". Moreover, these various pathologic stimuli have been reported to provoke oxidative stress as well as ER stress. In this study, we used the mice sensitized with OVA and LPS and then challenged with OVA (OVALPS-OVA mice) for elucidation of the relationship between mitochondrial ROS and ER stress in bronchial asthma. The OVALPS-OVA mice showed that the expression of ER stress markers and the protein levels of un-folded-protein response (UPR)-related marker in lung tissues were significantly increased after OVA challenge. In addition, we visualized the localization of mitochondrial ROS in BAL cells isolated from OVALPS-OVA mice using confocal microscopy; the significant increase in mitochondrial ROS in BAL cells was observed after OVA challenge. Our results also showed that Necrox-5 or 4-PBA significantly reduced the increases in ER stress, mitochondrial ROS, inflammatory cytokines, airway in-flammation, and bronchial hyperresponsiveness. These findings suggest that mitochondrial ROS and ER stress plays an im-portant role in the induction and maintaining allergic airway diseases synergistically.
Kang, Min-Jung,Lee, Hye-Im,Shin, Sang-Mi,Yoo, Hye-Hyun,Kwon, Oh-Seung,Jin, Chang-Bae,Chung, Bong-Chul Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.10
The objective of this study was to validate performance characteristics of the Access 2 (Beckman coulter) system for hCG assays for use as a confirmation test for doping control. The Access 2 assay was linear up to 500 IU/L. The correlation coefficient was higher than 0.999, and the sensitivity of the linearity was 0.82. There were no false positive or false negative cases. LOD was 0.59 IU/L. The method was robust when performed by different people. Repeatability and reproducibility were below 7%. We compared reproducibility and recoveries of Access 2 and Elecsys 2010. Access 2 demonstrated higher reproducibility than Elecsys 2010. Recoveries (accuracy) of Access 2 were between 85 and 105%. Recoveries for Elecsys 2010 were between 91 and 104%.
Kang, Eun-Sung,Kim, Chae-Young,Kim, Seon-Beom,Im, Se-Jin,Yang, Se-Hwan,Sung, Young-Chul,Kim, Byong-Moon 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.3
This study investigated the pharmacokinetic profile and biodistribution of HB-110, a novel HBV therapeutic vaccine candidate, in mice. HB-110 was rapidly degraded in the blood after i.v.injection with a half-life of 1.9${\pm}$0.083 min, and was no longer detected at 60 min except in one individual near the detection limit. In the i.m. injection, plasmid DNA was detectable at the injection site until ff days after administration, but the amounts were just above the detection limit. The blood concentration of HB-110 showed a maximum of 604 pg/mL at 15 min after i.m.injection, which was followed by degradation to undetectable levels at 90 min. The plasmid DNA in tissues peaked at 90 min after administration. The highest concentration of plasmid DNA was detected in the liver (24.172 pg/mg tissue), and considerable amounts were also observed in the lung (9.467 pg/mg tissue) and spleen (7.688 pg/mg tissue). The amount of plasmid DNA in tissues was 2 to 3 orders of magnitude lower than in the injection site at the same time points. The HB-110 concentration in tissues, including gonads, decreased rapidly and was undetectable 24 h after administration.
Synthesis and Cytotoxicity of New Aromatic Ceramide Analogs with Alkylsulfonamido Chains
Kang, Joo-Sung,Kim, Seung-Yong,Choi, Su-Hang,Lim, Se-Jin,Im, Chae-Uk,Yim, Chul-Bu,Kim, Kyoung-Won 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5
A series of D-erythro ceramide analogues, N-(2S,3R,4E)-1 ,3-dihydroxy-5-phenyl pent4-en-2-yl alkyl sulfonamides, were synthesized and evaluated for their in vitro cytotoxic activities against five human tumor ceil lines. The aromatic sullen amido ceramide analogue (10f)showed more potent cytotoxic activity than that of the B13, indicating that a sulfonamide group appears to serve as a bioisostere of an amide in drug design. Variations in the alkyl sulfonyl chain length signifcantly influenced the cytotoxic activity of the sulfonamido ceramide analogues, but the introduction of a para halogen on the phenyl ring of aromatic ceramide analogues had no affect on the activity.
Im, Hyung-Jun,Kim, Yong-Il,Paeng, Jin-Chul,Chung, June-Key,Kang, Soon-Beom,Lee, Dong-Soo The Korea Society of Nuclear Medicine 2012 핵의학 분자영상 Vol.46 No.1
Purpose : Retrocrural lymph nodes (RCLNs) communicate with retroperitoneal and posterior mediastinal LNs. It is possible that, when RCLNs are involved, supra-diaphragmatic extension will occur in abdomino-pelvic cancers. The authors investigated performance of $^{18}F$-FDG PET/CT to diagnose RCLN metastasis and whether RCLN metastases were associated with supra-diaphragmatic lymphatic metastases of ovarian cancer. Materials and methods : Sixty-seven patients with stage IV ovarian cancer who had undergone $^{18}F$-FDG PET/CT were included in this retrospective study. Diagnostic performance of $^{18}F$-FDG PET/CT for RCLN metastasis was evaluated. Patients were divided into two groups by presence or absence of supra-diaphragmatic LN metastasis. The prevalences of RCLN metastasis between the two groups were compared and the odds ratio was calculated. Results : Sensitivity and specificity of $^{18}F$-FDG PET/CT for RCLN metastasis were 96.3 and 100%, respectively. Of the 67 study subjects, 27 patients had RCLN metastases (40.3%). Fifty patients had supra-diaphragmatic LN metastases. $^{18}F$-FDG PET/CT showed 26 RCLN metastases in patients with supra-diaphragmatic LN metastases (54.5%), and only 1 in patients without supra-diaphragmatic LN metastasis (5.9%), and the difference between two groups was statistically significant (P<0.05). The odds ratio that patients with RCLN metastasis would have supra-diaphragmatic LN metastasis was 17.3 (95% confidence interval=2.1 to 140.9, P=0.008). Conclusion : Performance of $^{18}F$-FDG PET/CT to diagnose RCLN metastasis was excellent. RCLN metastasis revealed by $^{18}F$-FDG PET/CT was strongly associated with supra-diaphragmatic LN spread of ovarian cancer. Thus, RCLN metastasis could be used as a predictor of supra-diaphragmatic lymphatic metastasis of ovarian cancer.
미스트 분무 및 스프링클러 관수가 단동 플라스틱 하우스에 미치는 냉각효과
강정윤,임홍성,최동호,허종철 濟州大學校 産業技術硏究所 2000 산업기술연구소논문집 Vol.11 No.1
As crops recently can be cultivated all the year in greenhouses. we need to improve the environment in which crops are able to grow in the summer. Therefore. in this study. we quantitatively got the cooling effects of 4 single span plastic greenhouses in the following ways: by spraying mist or sprinkler. or by opening the side window. and with those variables. we simultaneously did experiments at 4 greenhouses under equivalent conditions to reduce the temperature of the greenhouses in the summer. The results of the experiments are as following : 1) The temperatures of the greenhouses were effectively reduced by both spraying mist and sprinkler. 2) When the mist is intermittently sprayed. the indoor temperature has decreased by 4 degrees centigrade. meanwhile. in the greenhouse to make experiments using sprinkler. the temperature of that has been reduced by 2 degrees centigrade. Therefore. we can see the cooling effects of smaller mist in size as more effective than those of sprinkler. 3) When the greenhouse is naturally ventilated by opening the side window. we have to apply another cooling system because the indoor temperature is higher than the outdoor one.