악성 흉막삼출액에서 Bleomycin을 이용한 흉막유착설의 시술효과

박정현,김학렬,양세훈,정은택 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.1

목적: 악성 흉막삼출액이 반복적이고 지속적으로 발생하는 경우, 호흡곤란, 흉통등의 증상을 유발시키므로 흉막경화제에 의한 흉막유착술을 시행할 수 있다. 흔히 사용되던 talc의 심각한 부작용이 보고되고 있고, doxycycline 주사제도 국내에서 발매되지 않고 있다. 이에 연자들은 항암제로 소개된 bleomycin을 이용한 흉막유착술의 단기와 중기효과, 부작용 및 생존율 등을 전향적으로 확인하였다. 방법: 2000년 1월부터 2001년 12열까지 원광대학교 부속병원에 내원하여 반복적이고 지속적인 악성 흉막삼출액으로 흉막유착술이 필요한 26명의 환자를 대상으로 특별한 전 처치 없이 bleomycin 60 unit를 식염수와 함께 흉강 내 주입 후, 1일 후의 흉관에 의한 배액량을 확인하고 부작용을 관찰하였으며, 1개월 후에 흉부 방사선상으로 재 저류 유무를 확인하였다. 또한 시술 반응군과 비반응군으로 나누어 중앙생존기간을 비교분석 하였다. 결과: 시술 1일 후 26예중 10예(38%)에서 배액이 없었고, 8예(31%)에서 배액량이 100ml이하였으며, 8예(31%)는 100ml이상이었으나 시술 전보다 감소하였으며 증가된 경우는 없었다. 유의할만한 부작용은 4예(고열 3예, 흉통 1예)에서 발생하였으나, 대증요업으로 조절되었다. 시술 1개월 후 흉부 방사선과 26예중 9예(35%)는 재 저류액이 없었고, 10예(38%)는 저류액이 있으나 시술 전보다 감소하였으며, 1예(4%)는 이전과 변화가 없었고, 6예(23%)는 시술 전보다 저류액이 증가하였다. 시술 후 단기 및 중기효과를 반응군과 비반응군으로 나누어 중앙생존기간으로 양군간을 비교하였을 때, 반응군에서 연장이 되었으나 통계적으로 유의한 차이는 없었다. 결론: 이상의 결과로서 bleomycin에 의한 흉막유착술은 다른 경화제보다 특별한 전 처치가 필요 없는 간편하고, 비교적 부작용이 적은 안전한 방법이며, 효과적인 치료성적을 보이는 시술로 사료된다. Background : Malignant pleural effusions are common and significant problems in patients with advanced malignancy. Repeated thoracentesis provides temporary symptomatic improvement, but most patients progressively cause dyspnea or pleuritic pain. So pleurodesis with sclerosing agent is effective as a palliative treatment. There are many drugs used as pleural sclerosing agents. But doxycycline hasn't been used in Korea since 1999. And recently talc has been reported serious side effects. In this study, pleurodesis with bleomycin was prospectively investigated for its short term and middle period effects and its side effects. Methods : Twenty-six patients with malignant pleural effusion were prospectively analyzed to estimate the effects of bleomycin pleurodesis. Without special management before the procedure, 60 units of bleomycin in 50-100ml of normal saline were instilled into the pleural space via a small bore catheter(8-10 F). Andthen repeated positional changes and rotations were done for 2 hours. We measured the drainage amount and evaluated the side effects after 24 hours and checked the existence of the effusion by chest radiography after 1 month. Results : After 24 hours, the drainage amount showed that the responder group(none or ≤ 100ml) was 69%, and that the nonresponder group(> 100ml) was 31%. Side effects (high fever in 3 cases and severe pleuritic pain in 1 case) were 15%(4/26), but patients recovered easily by symptomatic management. After 1 month, chest radiography showed that the response rate(complete or partial response) was 73%. Differences of survival times between responder and nonresponder group was not statistically significant. Conclusions : We are suggest that bleomycin as a pleural sclerosing agent is a relatively simple, safe and effective agent. But a prospective study with a larger number of patients must be warranted.

한국산 가시톡토기과의 분류학적 검토

이병훈,이원구,김학렬 全北大學校 生物學硏究所 1984 生物學硏究年報 Vol.5 No.-

A preliminary test concerning variabilities of some Tomoceridae species of collembola has been made using scanning electron microscopy and enzyme analysis. No definite taxono mical characters were detected in the integument of 6 species belonging 2 subgenera of Tomocerus genus. The zymograms obtained by electrophoresis, however, revealed that th subgeneric characters at times should be questioned for its monophyletic meaning as suggested by those of Tomocerus (Tomocerus) kinoshitai showing a particular pattern and Tomocerus (Tomocerina) liliputanus for its apprectable variability. The distinctive zymograms of T.laxalamella might also justify the creation of a new subgenus when considered in combination with the morphological characteristics of mucron. Neither population nor interspecific difference were observed, however, in and between T. cuspidatus and T. ocreatus, which would remain problematic and necessitate further works to confirm. Tomocerus laxalamella and T. liliputanus showed more of less variabilites whereas Homidia koreana of Entomobryidae, used as out-group for comparison, displayed a prominent individual variations in a few loci.

폐암으로 오인된 이물질에 의한 기관지 육아종증 1예

정종훈,김소영,김휘정,김학렬,양세훈,정은택 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

자발성 기흉을 동반한 폐림프관평활근종증 1예

안용환,정종훈,김소영,김휘정,김학렬,양세훈,최순호,문형배,조향정,정은택 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

Resection of an endobronchial hamartoma by cryotherapy

( Hak Ryul Kim ),( Keum Ha Choi ),( Eun Taik Jeong ),( Ki Eun Hwang ) 대한내과학회 2016 The Korean Journal of Internal Medicine Vol.31 No.4

Purification and Characterization of a Novel Thermostable Alpha-amylase from Korean Pine Seeds

Hak Ryul Kim,Md. Abul Kalam Azad,Jae Han Bae,Min Jung Suh 한국응용생명화학회 2008 Applied Biological Chemistry (Appl Biol Chem) Vol.51 No.5

The Korean Academy of Tuberculosis and Respiratory Diseases : Slide Session ; OS-066 : Lung Cancer ; Down-Regulated SIRT1 by Non-Steroidal Anti- Infl ammatory Drugs Is to Inhibit TGF-ß1-Induced Epithelial-Mesenchymal Transition and to Suppress Migration a

( Hak Ryul Kim ),( Ki Eun Hwang ),( Eun Taik Jeong ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Non-steroidal anti-in ammatory drugs (NSAIDs) has been reported to suppress lung cancer invasion and metastasis. However, the mechanism in lung cancer epithelial-mesenchymal transition (EMT) by NSAIDs is not clearly known. The class IIIdeacetylase sirtuin 1 (SIRT1) possesses both pro- and anticarcinogenic properties. In this study, we investigated the role of NSAIDs as an inhibitor of TGF-ß1-induced EMT, and the underlying mechanisms of suppressing lung cancer migration and invasion by celexiband sulindac. Methods: We evaluated the effi cacy of celecoxib and sulindac in TGF-ß1-induced EMT. EMT-related molecular alterations were detected by western blotting. Electric cell-substrate impedance sensing (ECIS) was used to evaluate the migration and invasion of A549 cells. The matrix metalloproteinase (MMP) gelatinolytic activity was determined by gelatin zymography, and protein expression was measured by western blotting. In addition,SIRT1 was knocked down or overexpressed to determine its role in preventing TGFß1- induced EMT by celecoxib or sulindac. Results: Celecoxib was more effective in preventing TGF-ß1-induced EMT, as compared with sulindac treatment, indicating upregulation of the epithelial marker, E-cadherin, anddownregulation of N-cadherin, mesenchymal and transcription factors. Moreover, ECIS assay showed that celecoxib and sulindac could inhibit TGF-ß1-enhanced migration andinvasion of A549 cells. Increment in MMP-9 expression compared to that of MMP-2 following activation of TGF-ß1 was also observed. However, treatment with celecoxib orsulindac inhibited MMP-9 expression. SIRT1 downregulation enhanced reverse of TGFß1- induced EMT by celecoxib or sulindac. In contrast, SIRT1 upregulation had a relevant role in TGF-ß1-induced EMT.Conclusions: Celecoxib and sulindac can inhibit TGF-ß1-induced EMT and suppress lungcancer migration and invasion via down-regulation of SIRT-1. SIRT1 is positive regulator of TGF-ß1-induced EMT and potential therapeutic target to reverse EMT and to prevent lung cancer progression.

Free Paper Presentation : OS-66 ; Down-Regulated SIRT1 by Non-Steroidal Anti-Inflammatory Drugs Is to Inhibit TGF-β1-Induced Epithelial-Mesenchymal Transition and to Suppress Migration and Invasion in Lung Cancer

( Hak Ryul Kim ),( Ki Eun Hwang ),( Eun Taik Jeong ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-

Background: Non-steroidal anti-inflammatory drugs (NSAIDs) has been reported to suppress lung cancer invasion and metastasis. However, the mechanism in lung cancer epithelial-mesenchymal transition (EMT) by NSAIDs is not clearly known. The class III deacetylase sirtuin 1 (SIRT1) possesses both pro- and anticarcinogenic properties. In this study, we investigated the role of NSAIDs as an inhibitor of TGF-β1-induced EMT, and the underlying mechanisms of suppressing lung cancer migration and invasion by celexib and sulindac. Methods: We evaluated the efficacy of celecoxib and sulindac in TGF-β1-induced EMT. EMT-related molecular alterations were detected by western blotting. Electric cell-substrate impedance sensing (ECIS) was used to evaluate the migration and invasion of A549 cells. The matrix metalloproteinase (MMP) gelatinolytic activity was determined by gelatin zymography, and protein expression was measured by western blotting. In addition, SIRT1 was knocked down or overexpressed to determine its role in preventing TGF-β1-induced EMT by celecoxib or sulindac. Results: Celecoxib was more effective in preventing TGF-β1-induced EMT, as compared with sulindac treatment, indicating upregulation of the epithelial marker, E-cadherin, and downregulation of N-cadherin, mesenchymal and transcription factors. Moreover, ECIS assay showed that celecoxib and sulindac could inhibit TGF-β1-enhanced migration and invasion of A549 cells. Increment in MMP-9 expression compared to that of MMP-2 following activation of TGF-β1 was also observed. However, treatment with celecoxib or sulindac inhibited MMP-9 expression. SIRT1 downregulation enhanced reverse of TGF- β1-induced EMT by celecoxib or sulindac. In contrast, SIRT1 upregulation had a relevant role in TGF-β1-induced EMT. Conclusions: Celecoxib and sulindac can inhibit TGF-β1-induced EMT and suppress lung cancer migration and invasion via down-regulation of SIRT-1. SIRT1 is positive regulator of TGF-β1-induced EMT and potential therapeutic target to reverse EMT and to prevent lung cancer progression.

Comparison of Vitamin Contents and Organoleptic Characteristics of Powdered Cheongkukjang Dried by Different Drying Methods

Hak-Ryul Yang,Sang-Soon Park,Jang-Woo Lee,Keun-Bo Lee,Myung-Kyu Han 한국식품영양학회 2009 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2009 No.06

Carbohydrates and Inorganic Ions in Haemolymph and Fat Body during Metamorphosis of the Silkworm, Bombyx mori L.

Kim, Hak-Ryul,Yoe, Sung-Moon,Yu, Jeong-Heui,Kim, Kyung-Mi 한국곤충학회 1983 Korean journal of entomology Vol.13 No.2

누에의 변태 기작을 밝히기 위한 일환으로 종령유충에서 용시기에 걸쳐 총 탄수화물, Glycogen, Trehalose, Glucose 및 Sodium과 Potassium 농도를 혈림프와 지방체에서 측정, 비교하였다. 총 탄수화물 함량은 종령유충 6일과 용화후 5일에 혈림프에서 peak를 나타냈으며 지방체에서는 종령유충말과 용화 직후에 peak를 보여 주었다. Glycogen은 혈림프에서는 미량 존재하나 지방체에서는 종령유충말과 용화 직후에 peak를 나타냈다. Trehalose는 혈림프의 주요 탄수화물로써 전용기 및 용화후 5일에 peak를 보여 주었으나 지방체에서는 미량 존재하며, Glucose는 혈림프와 지방체에서 모두 미량 함유되어 있다. 혈림프내의 sodium 농도는 종령유충 및 전용기에 걸쳐 비교적 일정하나 용화 직후 약간 감소하고 용화후 5일에 peak를 나타낸 반면 potassium 농도는 용화유충기와 용기에 걸쳐 비교적 일정하게 높은 농도를 유지하였다. Total carbohydrates, glycogen, trehalose, glucose, sodium, and potassium were measured in haemolymph and fat body over the period of last larval to pupal stage of Bombyx mori to trace the metabolism of carbohydrate during pupation. Total carbohydrates content show the peaks at 6 day last larvae and 5 day pupae in haemolymph but late last larvae and early pupae in fat body. Glycogen is present very small in haemolymph but large in fat body with the peaks at late last larvae and newly ecdysed pupae. Trehalose is mostly present in hae-molymph with the peaks at pharate pupae and 5 day pupae but small in the fat body Glucose appears in very small amounts in haemolymph and fat body. Sodium remains relatively constant throughout the period of last larvae to early pupae with a little decrease just after pupations and the peak at 5 day pupae. However, potassium maintains relatively high constant level over the period of last larval to pupal stage.