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김소희,김순선,이규식,손경희,곽승준,채수영,안상미,허만욱,박귀례 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-
임신중에 만성적으로 음주한 산모의 태아는 중추신경계 손상으로 인한 특징적인 이상증상인 소두증 정신발육장애 등 태아 알코을 증후군(fetal alcoho3 syndrome, F,LSB이 나타나는 것으로 알려져 있으냐 분자생물학적 측면에서의 유발기전은 명확하게 밝혀져 있지 않다. 든 연구예서는 in vitro 단기 발생독성시험법 중 하나인 전배자배양댑을 사용하여 랫드 배자의 )1관 발생기에 알코을 및 알코올의 주 군사 체인 아세트알데히드를 처리하여 기형 유발 여부를 관찰하였다. 또한 이들 물질의 기형 유발 기전을 규명하고자 microarray 깡법으로 발현이 변화되는 유전자를 검색하였으며, 알코을 처리로 발현이 변후 되는 유전자의 정확한 확인을 위하여 northern blot analysis 실시 중이다. 임신 9.5일의 랫드 배자에 0.1, 0.5, 1.0% 에란올을 처리하였을 때 성장부진, 뒤틀리거나 꼬렬라진 꼬리, open caudal neura tube, open neural tube, 불완전찬 반전, 불규칙적이고 비정상적인 체절과 짧은 꼬리 등의 이상이 나티났다. 임신 9.5일의 랫드 배자에 Sr10-4, 8.3×10-4, 2.SxIO-3% 아세트알데히드를 처리시 래antois caudal neura3 tube의 발달저해, somite 수 감소, 꺽인 꼬리 등의 이상이 나타났다. Control과 에탄을 처리한 배자로부터 total RNA를 추출하고 poll· A--RNA를 분리한 후, reverse transcriptase를 사용하여 C?3 혹은 Cl·5 형광염료로 tag된 nucleotide로 표지된 probe를 만들어 등량을 시판중인 'toxicotogy chip'의 틴991개의 CDNA 형태의 유전자와 hybr겨iaation시켰다. 에탄을 처리에 의해 'serum deprivation response' gene등의 발현이 유도되었으며, 'rat transcription factor MaflmRNA'등의 발현은 저해되었다. It is reported that alcohol drinking by pregnant mothers often leads to abnormal fetal development including fetal alcohol syndrome(FAS) in both humans and experimental animals. The FAS is characterized by prenatal and postnatal growth retardation, distinct craniofacial dysmorphology, and central nervous system dysfunctions such as mental retardation. However, molecular mechanisms for FAS is not completely elucidated as yet. In this study, developmental toxicity of ethanol and its metabolite, acetaldehyde were evaluated using whole embryo culture. In addition genes inducing FAS were investigated by the technique of cDNA microarray. Embryos treated with ethanol showed growth retardation, abnormal tail torsion, open neural tube, open caudal neural tube and abnormal somite at concentrations of 0.1∼0.5%. Also embryos treated with acetaldehyde showed growth retardation including allantois, open caudal neural tube, reduction number of somite and abnormal tail torsion at concertrations of 5×10^(-4)∼2.5×10^(-3)%. RNAs from embryos of control and ethanol treatment were reverse transcribed into cDNA tagged with Cy3 or Cy5 and hybridized with cDNA probe of rat in 'toxicology chip'. Ethanol 0.5% treatment resulted in alteration in expression of several genes including 'serum deprivation response', 'Cide-b' and 'check point suppressor 1'. Northern blot analysis is conducted for identification of genes related to FAS.
Poster Session 2 : Integration and biodistribution studies on eGFP retroviral vector in mouse
( Gyu Seek Rhee ),( Ji Hyun Seok ),( Soon Sun Kim ),( Bo Ra Kim ),( Seung Jun Kwack ),( Rhee Da Lee ),( Soo Young Chae ),( Young Hyuk Won ),( Seung Shin Yu ),( Dae Hyun Cho ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-
Chae, Myounghee,Rhee, Gyu-Seek,Jang, Ik-Soon,Kim, Kwangsoo,Lee, Ji-Hae,Lee, Seung-Yeul,Kim, Minjung,Yang, Junyoung,Park, Junsoo,Lee, Seung-Hoon Springer-Verlag 2009 Molecules and cells Vol.27 No.5
<P>The amphetamine derivative 3, 4-methylenedioxymethamphetamine (MDMA) has become a popular recreational drug, and has also been shown to cause serotonergic neurotoxicity. This report shows that MDMA impairs brain development in a whole mouse embryo culture. The results of quantitative real-time PCR analysis showed that autophagy-related protein 5 (Atg5) expression is elevated in mouse embryo and neuroblastoma cells after MDMA treatment. This elevated Atg5 expression interferes with the neuronal differentiation of neuroblastoma cells such as SH-SY5Y and PC12 cells. Thus, our results suggest that the use of MDMA during pregnancy may impair neuronal development via an induction of Atg5 expression.</P>
Myounghee Chae,Gyu-Seek Rhee,장익순,김광수,Ji-Hae Lee,Seung-Yeul Lee,Minjung Kim,Junyoung Yang,박준수,이승훈 한국분자세포생물학회 2009 Molecules and cells Vol.27 No.5
The amphetamine derivative 3, 4-methylenedioxymetham- phetamine (MDMA) has become a popular recreational drug, and has also been shown to cause serotonergic neurotoxicity. This report shows that MDMA impairs brain development in a whole mouse embryo culture. The results of quantitative real-time PCR analysis showed that autophagy-related protein 5 (Atg5) expression is elevated in mouse embryo and neuroblastoma cells after MDMA treatment. This elevated Atg5 expression inter-feres with the neuronal differentiation of neuroblastoma cells such as SH-SY5Y and PC12 cells. Thus, our results suggest that the use of MDMA during pregnancy may impair neuronal development via an induction of Atg5 expression.
Potential Estrogenic and Antiandrogenic Effects of Permethrin in Rats
KIM, Soon-Sun,LEE, Rhee-Da,LIM, Kwon-Jo,KWACK, Seung-Jun,RHEE, Gyu-Seek,SEOK, Ji-Hyun,LEE, Geun-Shik,AN, Beum-Soo,JEUNG, Eui-Bae,PARK, Kui-Lea 家畜繁殖硏究所 2005 Journal of Reproduction and Development Vol.51 No.2
<P>Many environmental chemicals including pesticides have been reported to possess hormonal activities, and thus are classified as endocrine disruptors. Permethrin, a synthetic pyrethroid insecticide, is used worldwide, which provides potential environmental exposure. However, relatively few studies have reported on hormonal activities, particularly estrogenic and androgenic activities of permethrin, and the results of these studies are in some respects contradictory. Therefore, this study investigated the potential estrogenic and androgenic activities of permethrin <I>in vitro</I> and <I>in vivo</I>. We conducted an uterine Calbindin-D<SUB>9k</SUB> (CaBP-9k) gene expression assay and an uterotrophic assay for estrogenic activity, and a Hershberger assay for androgenic activity. The CaBP-9k gene, one of the intracellular calcium binding proteins, is estrogen-responsive in the uterus. The rat uterotrophic and Hershberger assays are generally used as <I>in vivo</I> short-term screening assays for detecting the estrogenic and androgenic activities of chemicals, although these assays are still being validated by the Organization for Economic Cooperation and Development (OECD). Northern blot analysis showed the induction of uterine CaBP-9k mRNA level in response to permethrin as well as co-administration of permethrin with E2. In the uterotrophic assay using 18-day-old female rats, subcutaneous treatments with permethrin (10 to 800 mg/kg) for three days increased relative uterine wet weights, and E2-induced uterine weights. These effects were statistically significant at 800 and 200 mg/kg, respectively. Moreover, permethrin-induced uterine weights were inhibited by the co-administration of ICI 182,780, an antiestrogen. In the Hershberger assay, the administration of permethrin orally to testosterone propionate-treated castrated male rats led to statistically significant reductions in androgen-dependent sex accessory tissue (ventral prostate, seminal vesicles, levator ani and bulbocavernosus muscles, Cowper's gland and glans penis) weights at all doses tested (10, 50 and 100 mg/kg). These results suggest that permethrin might have estrogen-like effects on female rats, but antiandrogen-like effects on males.</P>
Kim, Hack-Seang,Rhee, Gyu-Seek,Oh, Ki-Wan The Korean Society of Ginseng 1995 Journal of Ginseng Research Vol.19 No.3
We have previously reported that the antagonism of U-50,488H-induced antinociception in mice pretreated with ginseng total saponins (GTS) Ivas abolished by pretreatment with a serotonin precursor, 5-hydroxytryptophan (5-HTP), but not by a noradrenaline precursor, L-dihydroxyphenylalanine (L-DOPA) in the tail flick test. In the present experiments, the effect of the same GTS on the development of tolerance to U-50,488H-induced antinociception was determined. GTS inhibited the development of tolerance to U-50,488H-induced antinociception. The inhibitory effect of GTS on the development of tolerance to U-50,488H-induced antinociception was reversed by 5-HTP, but not by L-DOPA. These findings suggest that the inhibitory effect of GTS on the development of tolerance to U-50,488H-induced antinociception is dependent on serotonergic mechanisms. Key words Ginseng total saponin, U-50,488H, tolerance, serotonin.