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      • KCI등재

        생쥐에서 치주조직 형성 과정에 따른 치주인대 세포-특이 PDLs17 단백질 발현 변화

        최치원,김은미,이명화,김흥중,박주철 조선대학교 구강생물학연구소 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.2

        PDLs17, PDL-specific gone, had previously identified the cDNA for a novel protein from cultured PDL fibroblasts using subtraction hybridization between gingival fibroblasts and PDL fibroblasts. The purpose of this study was to observe the immunohistochemical localization of PDLs17 on the developing periodontium of mice. Developing mice (post-natal mice; P4, P10, P15) were recovered and decapitated, and the heads were fixed overnight at in a freshly prepared solution of 4% paraformaldehyde. Some specimens were decalcified for 2-4 weeks in a solution containing 10% of the disodium salt of ethylenediamine-tetraacetic acid (EDTA). Next, tissues were dehydrated, embedded in paraffin and sectioned serially at 6㎛ in thickness. Polyclonal antiserum raised against PDLs17 peptides. SEAVHETDLHDGC, were made, and immunostained the tooth, periodontium, developing bone and bone marrow of developing mice. The results were as follows. 1. Immunohistochemical analysis showed that PDLs17 is Preferentially expressed in the developing tooth follicle, but not in tooth pulp and overlying gingival mucosa of 4-day-old developing mouse. PDLs17 also expressed in preosteoblast-likec ells in the developing alveolar bone of 4-day-old mouse. 2. PDLs17 expressions were observed in developing tooth follicle, tooth pulp and alvolar bone of 10-day-old developing mouse. 3. PDLs17 protein were observed in PDL cells, tooth pulp and surrounding alveolar bone of 15-day-old tooth-eloping mouse. However, there were no PDLs17 expression in ameloblasts and odontoblasts. These results suggested that PDLs17 might expressed at the initial stage of differentiation when the undifferentiated mesenchymal cells in the bone marrow and PDL, differentiatiation from tooth follicle. However, more research needs to be performed to gain a better understanding of the exact function of PDLs17 during the differentiation of tooth pulp cells and PDL cells.

      • 지문정보를 이용한 안전한 암호키 저장 시스템 개발

        최은화,서창호,임종태,김석우 公州大學校 工學硏究院 生産技術硏究所 2003 論文集 Vol.11 No.-

        Biometric encryption refers to a process of secure key management. Biometric encryption does not directly provide a mechanism for the encryption/decryption of data, but rather provides a replacement to typical passcode key-protection protocols. Although the process of biometric encryption can be applied to any biometric image, the initial implementation was achieved using fingerprint images.

      • 건강인에서 결핵균 30-kDa항원이 말초혈액 임파구증식에 미치는 영향

        최대경,고석신,김화중,조은경,백태현,신철식 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

        T lymphocytes are thought to play a central role in cell mediated immune response. To Study the T lymphocyte proliferative response to 30-kDa antigen purified from Mycobacterium tuberculosis H37Rv, peripheral blood lymphocytes isolated from healthy subjects were stimulated with the 30kDa antigen, crude antigen or PHA. Healthy subjects were divided into three groups so that PPD(+), PPD(±) and PPD(-). The proliferations of lymphocytes were measured by the incroporation of ^3H-thyrnidine. The results were summarized as follows: 1. For the PPD(+) person, at concentration of < 1 ㎍/ml, the proliferative response to 30-kDa antigen resulted in a mild reduction but crude antigen showed relatively rapid reduction. For the PPD(-) person, decreasing the concentration of the both antigens showed rapid reduction of proliferation 2. The lymphocytes proliferations to 30-kDa antigen and crude antigen were significantly increased in PPD(+) group when compared to those in PPD(-) group, but PHA response was no significant difference. 3. The sensitivity and specificity of lymphocyte proliferation assay to 30-kDa antigen and crude antigen when compared with skin test results were 92.3%, 100% and 76.9%, 50%, respectively.

      • KCI등재

        2004년 7월 봇돌바다의 썰물, 전류 및 밀물시 수온과 염분 분포

        최용규,조은섭,이용화,이영식 한국환경과학회 2005 한국환경과학회지 Vol.14 No.2

        Based on the observation on 20, 23 and 26 July 2004, the distributions of temperature, salinity and stratification was investigated in relation to ebb, turn of tide and flood. The results are as follows: 1) The high temperature and low saline water with 23.5-24.0℃ and 32.4~33.0psu existed at Naro Island. 2) The cold surface water below 21.00 and 33.0~33.4psu appeared in the area near Gae Island and Geumo Island. 3) The cold and saline water, below 24.0℃ at the surface and 17.0℃ near the bottom, 32.8~33.8psu at the surface and 33.8~34.0psu near the bottom, existed in Sori Island. These waters were more saline compared to the South Sea Coastal Water with about 31.8psu. This suggests that the oceanic saline water intruded into the Bottol Bada through the area near Sori Island. The stratification appeared during all the observation periods due to a high solar radiation of 22MJ/m², and a weak wind speed of 2.9m/s on the average while the mean speed of wind in July is around 3.9 m/s. It qualitatively suggested that the stratification was maintained during the observation periods because of a high solar radiation, a weak wind speed and intrusion of saline oceanic water.

      • KCI등재

        2004년 7월 봇돌바다의 영양염과 Chlorophyll-a의 단기 변동

        최용규,조은섭,권기영,이용화,이영식 한국환경과학회 2005 한국환경과학회지 Vol.14 No.4

        In order to study the temporal variations of nutrients and chlorophyll-a in the Bottol Bada, three field observations were carried out on 20, 23 and 26 July, 2004. The low N:P values exhibit nitrogen deficiency during the periods of observation. This result is not representative of typical summer environment in the southern coast of Korea. The possible mechanisms are as follows: 1) The freshwater inflow was not sufficient for the supply of nitrogen because the total precipitation was 11.9 mm in July, 2004. This amount is no more than 5% in normal precipitation in July. 2) There was an inflow of oceanic water under the subsurface into the Bottol Bada. Even though the oceanic water comprises more nutrients, it produces the stratification between the surface and the subsurface water and seems to prevent the supply of nutrinets to the surface layer. 3) The high chlorophyll-a concentration of 1.2 ㎍/L was shown near the narrow channel between Gae-do and Geumo-do. This seems to be resulted from the inflow of water from Gamak Bay.

      • KCI등재

        Chlorhexidine 처리가 상아질 접착제의 미세인장결합강도에 미치는 영향

        오은화,최경규,김종률,박상진 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.2

        본 연구는 상아질 혼성층의 교원섬유를 가수분해하는 효소인 MMPs (Matrix metalloproteinses) 억제제로 알려진 chlorhexidine (CHX)을 적용 후 결합강도를 측정하였으며, 이를 각각 열순환 처리 후 결합강도를 측정하였다. 또한 주사전자현미경으로 접착계면메서의 파괴 양상을 비교 분석하였다. 우식이 없는 발거한 32 개의 제 3 대구치의 교합면 상아질을 노출시키고 GI그룹에서는 dentin conditioner를 처리 후 2% chlorhexidine을 적용시키고, 산부식 접착제 그룹에서는 인산 산부식을 시행하고 2 % chlorhexidine을 적용 후 3단계 산부식형 상아질 접착제 (Scotchbond Multipurpose, SM), 2단계 산부식형 상아질 접착제 (Single Bond, SB)를 도포하고, 자가부식 접착제 그룹에서는 2 % chlorhexidine 적용 후 자가부식 상아질 접착제 (Clearfil Tri-S, TS)를 도포한다. 이후 복합 레진 (Z-250)과 GI (Fuji-II LC)를 충전한 시편을 1 ㎟의 단면을 갖는 beam으로 제작하여 열순환 하지 않거나, 10,000 회 열순환 (5 ~ 55 ℃)하였다. Universal testing machine (EZ-test: Shimadzu, Japan)에서 cross head speed 1 mm/min로 인장력을 가하여 , 미세인장결합강도를 측정하였다. 실험 결과는 유의수준 0.05 1evel에서 two-way ANOVA를 이용하여 통계분석하였다. 그 후 파절된 시편의 파괴 양상을 현미경 (SEM)으로 관찰하여 다음과 같은 갈론을 얻었다; 1. 2 % CHX을 적용한 모든 실험군에서 상아질과의 미세인장결합강도가 증가하였고, 열순환은 상아질과의 미세인장 결합강도를 감소시켰다 (P>0.05). 2. CHX 적용 후 열순환 한 군은 CHX을 적용하지 않고 열순환한 군에 비하여, 상아질과의 미세인장결합강도가 높았으며 , 특히 GI와 TS군에서 유의한 차이를 나타내었다 (P<0.05). 3. 파괴 양상 분석 결과, 혼성층에서의 접착성 파괴를 보이며, CHX을 적용하면 혼성층 기저부에서 상부로 파괴 부위가 옮겨가는 양상을 나타내었다. 이상의 연구 결과를 토대로, MMPs 억제제인 2 % CHX은 글래스 아이오노머 시멘트와 상아질 접착제의 초기 미세인장결합강도에는 영향을 미치지 않으며 ,CHX 적용이 접착 내구성을 유지하는데 도움이 되었다. The purpose of this study was to evaluate the effect of chlorhexidine (CHX) on microtensile bond strength (μTBS) of dentin bonding systems. Dentin collagenolytic and gelatinolytic activities can be suppressed by protease inhibitors. indicating that MMPs (Matrix metalloproteinases) inhibition could be beneficial in the preservation of hybrid layers. Chlorhexidine (CHX) is known as an inhibitor of MMPs activity in vitro. The experiment was proceeded as follows : At first, flat occlusal surfaces were prepared on mid-coronal dentin of extracted third molars. GI(Glass lonomer) group was treated with dentin conditioner, and then, applied with 2 % CHX. Both SM (Scotchbond Multipurpose) and SB (Single Bond) group were applied with CHX after acid-etched with 37% phosphoric acid. TS (Clearfil Tri-S) group was applied with CHX, and then, with adhesives. Hybrid composite Z-250 and resin-modified glass ionomer Fuji-II LC was built up on experimental dentin surfaces. Half of them were subjected to 10,000 thermocycle, while the others were tested immediately. With the resulting data, statistically two-way ANOVA was performed to assess the μTBS before and after thermocycling and the effect of CHX. All statistical tests were carried out at the 95 % level of confidence. The failure mode of the testing samples was observed under a scanning electron microscopy (SEM). Within limited results, the results of this study were as follows; 1. In all experimental groups applied with 2 % chlorhexidine, the microtensile bond strength increased, and thermocycling decreased the microtensile bond strength (P > 0.05). 2. Compared to the thermocycling groups without chlorhexidine, those with both thermocycling and chlorhexidine showed higher microtensile bond strength, and there was significant difference especially in GI and TS groups.

      • 사용자 인식 스마트 홈 미러

        최건영,고성화,김종옥,박은서,김완규,백경동,박동규 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

        The Internet of Things, the core of the Fourth Industrial Revolution, is growing by incorporating various technologies into various fields. Among them, smart home systems that apply IoT technology to home environments are being actively studied. The home hub market, a means of communication for smart homes, is also developing at the same time. As the home hub market develops, security threats are also increasing accordingly. In this paper, 'Smart Home Mirror' is implemented by combining mirror and IoT technologies so that users can use them to communicate with smart home applications and other devices. To meet security issues, users in it are recognized by using face recognition technology and AES between all data communications in it is applied. The proposed device’s usefulness is approved by implementing it in smart home environments.

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