데페록사민 전처치가 토끼 심근경색 크기의 감소에 미치는 효과

양관모,오동렬,박승현,박규남,이원재,김형국,황두영,최승필,채장성 대한응급의학회 1998 대한응급의학회지 Vol.9 No.4

Background: Reperfusion of ischemic myocardium has been postulated to result in a specific oxygen radical mediated tissue injury. Iron may liberate during ischemia and we hypothesized that administration of the iron chelator, deferoxamine during ischemia would result in improved recovery after postischemic reperfusion. Purpose: To test whether iron-catalyzed processes contribute to myocardial necrosis during ischemia and reperfusion, deferoxamine was administered to block iron catalyzed hydroxyl radical formation in rabbits. Methods: Eleven rabbits were divided into two groups : control group (n=5) and deferoxamine pretreatment group (n=6). The left circumflex coronay artery was ligated for 30 minutes and reperfused for 180 minutes. Area at risk (AR) was measured by non-stained area with methylene blue injection into left atrium after left circumflex coronary artery ligation. Infarct size was measured by weighing after triphenyltetrazolium chloride staining. Heart rate was measured using electrocardiographic recording and systemic blood pressure was monitored by pressure transducer connected to the catheter in the left ventricle. Results: 1. There was no significant difference of heart rate and blood pressure in deferoxamine pretreatment group compared with control group. 2. There was significant decrease of serum iron concentration after continuous infusion of deferoxamine compared with serum iron concentration before ligation of coronary artery(P<0.05). 3. There was no significant difference of area at risk between control and deferoxamine pretreatment group. 4. Area at necrosis to area at risk was significantly reduced in deferoxamine pretreatment group compared with control group(P<0.05). The results suggest that deferoxamine infusion prior to coronary artery occlusion has a significant benefit in reducing infarct size in this model.

참깨 立枯性 病原菌에 관한 硏究

李斗珩,梁壯錫,韓東旭 서울市立大學校 1986 論文集 Vol.20 No.2

Damping-off and wilting diseases in sesame plant(Sesamum indicum L.) occurring severely in Korea have been studied for incidences of disease from seeds, diseased plants and soils. Alternaria longissima, A. tenuis, Aspergillus. spp, Cephalosporium sp., Cuvuaria lunata, Drechslera sp.,sp., Fusarium equiseti, F. moniliforme, F. semitectum, F. solani, Mucor sp., Myrothecium roridum Penicillium spp., Phoma sp., Rhizopus sp., were saprophytic fungi and Alternaria sesami, A. sesamicola, Macrophomina phaseolina, Rhizoctonia solani were pathogenic fungi detected from 66 seed samples of sesame. Initial symptoms caused by pathogenic fungi on seedlings from naturally infected seed by the test tube agar method have been described and discussed. Except Alternaria sesamicola, A. tenuis and Macrophomina phaseolina, all fungi were completey wiped out the infection by pretreatment with Benlate-T. The pathogenic fungi isolated from sesame soil were Fusarium oxysporum and Rhizoctonia solani. F.oxysporum occurred less frequently in upland rice and ground nut soils than in sesame soil, but R. solani not decreased by upland rice and ground nut soils. The differences of the isolation frequency of F.oxysporum and the incidence of the wilting plants were found among the fields which varied in their cropping histories. F.oxysporum isolated most frequently from stems and roots of wilting sesame plants followed by R. solani. Nematodes also isolated from the wilting sesame plants.

Clomiphene citrate에 의한 배란유도 후의 임신율에 관한 연구

임춘근,이민용,권경익,박세출,이두룡,남동호,양숙경 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.3

From March 1990 to February 1993, we evaluated completely follow-uped 68 cases of amenorrhea, oligomenorrhea, infertility(anovulation). The ratio of primary to secondary is 31: 37. Ovulation rate and pregnancy rate of 68 cases which were completely treated with clomphene citrate is 72%, 71.8% respectively. Among these 23 pregnat cases, 19 cases were full term delivered, 1 case is ongoing pregnant, and 3 cases were spontaneously aborted. No one of multiple pregnancy and congenital abnormality was noted.

Response of Grafted Watermelon Leaf Proteome Changes to Rootstock Genotypes

Dong Dong Yang,Hong Mei Du,Dan Feng Huang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Serum Carbohydrate Antigen 19-9 as an Indicator of Liver Metastasis in Colorectal Carcinoma Cases

Dong, Hang,Tang, Jie,Li, Long-Hao,Ge, Jun,Chen, Xin,Ding, Jing,Men, Hai-Tao,Luo, Wu-Xia,Du, Yang,Li, Cong,Zhao, Feng,Chen, Ye,Cheng, Ke,Liu, Ji-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Purpose: The liver is the organ to which colorectal carcinomas (CRCs) most commonly metastasize, and surgical resection has been established as the most effective and potentially curative treatment for CRC with liver metastasis (LM). Therefore, surveillance of LM is vital for improvement of prognosis of CRC patients. In this study, we aimed to explore the potential value of carbohydrate antigen 19-9 (CA 19-9), carcinoembryonic antigen (CEA), and marker enzymes in indicating LM with CRC. Methods: Three groups of eligible patients with metastatic cancers were retrospectively included: CRC patients with LM (CRC-LM) or without LM (CRC-NLM), and non-CRC patients with LM (NCRC-LM). All metastatic lesions were identified by CT or MRI. Data on characteristics of the patients, the primary site, the locations of metastasis, CA 19-9, CEA, and biochemical parameters were collected for analysis. Results: A total of 493 patients were retrospectively included. More alcohol consumption was found in CRC-LM than CRC-NLM. Some biochemical enzymes were found to be significantly higher in groups with LM than without (CRC-LM or NCRC-LM v.s CRC-NLM). Both CEA and CA 19-9 were much higher in CRC-LM than CRC-NLM or NCRC-LM. For CRC patients, CA 19-9, ${\gamma}$-glutamyl transpeptidase, CEA and alcohol consumption were identified as independent factors associated with LM. Conclusion: Our analysis suggested the CA 19-9 might be a potential valuable indicator for LM of CRC in the clinic.

Omi inhibition ameliorates neuron apoptosis and neurological deficit after subarachnoid hemorrhage in rats

Du Yuanfeng,Yang Dingbo,Dong Xiaoqiao,Du Quan,Wang Ding,Shen Yongfeng,Yu Wenhua 한국유전학회 2021 Genes & Genomics Vol.43 No.12

Background Subarachnoid hemorrhage (SAH) is a severe neurological emergency, resulting in cognitive impairments and threatening human's health. Currently, SAH has no efective treatment. It is urgent to search for an efective therapy for SAH. Objective To explore the expression of Omi protein after subarachnoid hemorrhage in rats. Methods SAH rat model was established by injecting blood into the prechiasmatic cistern. Neurological defcit was assessed by detecting neurological defcit scores and brain tissue water contents. Apoptotic cells were evaluated by TUNEL staining and IHC staining. Omi and Cleaved caspase 3 expressions in nerve cells were determined by double staining using IF. Apoptosis-related proteins were measured by Western blotting assay. Results SAH rat model was successfully established, showing more apoptotic cells and high neurological defcit scores in SAH rat. In SAH rat model, Omi expression in nerve cells was elevated and the upregulation of Omi mainly occurred in cytoplasm, accompanied by the degradation of XIAP and the increased cleaved caspase 3/9 and cleaved PARP. Once treated with UCF-101, a specifc inhibitor of Omi, the increased cell apoptosis, left/right brain moisture contents and neurological defcits were notably reversed in SAH rat brain. Of note, SAH-induced the increases of apoptosis-related protein in nerve cells were also rescued by the administration of UCF-101. Conclusions UCF-101-mediated Omi inhibition decreased the degradation of XIAP and subsequently inhibited the activation of apoptosis-related proteins, decreased nerve cell apoptosis, leading to the improvement on early brain injury in SAH rat. UCF-101-based Omi inhibition may be used to treat SAH with great potential application.

MicroRNA-27a Inhibits Cell Migration and Invasion of Fibroblast-Like Synoviocytes by Targeting Follistatin-Like Protein 1 in Rheumatoid Arthritis

Dong-liang Shi,Gui-rong Shi,Jing Xie,Xu-zhao Du,Hao Yang 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.8

Fibroblast-like synoviocytes (FLS) with aberrant expres-sion of microRNA (miRNA) are critical pathogenic regula-tors in rheumatoid arthritis (RA). Previous studies have found that overexpression or silencing of miRNA can contribute to the development of miRNA-based therapeutics in arthritis models. In this study, we explored the effects of miR-27a on cell migration and invasion in cultured FLS from RA patients. We found that miR-27a was markedly downregulated in the serum, synovial tissue, and FLS of RA patients. Meanwhile, the expression of follistatin-like protein 1 (FSTL1) was upregulated, which suggests that FSTL1 plays a key role in RA development. The results of a Transwell assay showed that miR-27a inhibited FLS migration and invasion. However, miR-27a inhibition promoted the migration and invasion of FLS. In addition, the down-regulated expression of matrix metalloproteinases (MMP2, MMP9, and MMP13) and Rho family proteins (Rac1, Cdc42, and RhoA) was detected after treatment with miR-27a in RA-FLS by quantitative reverse transcription-PCR and western blot analysis. Then, a luciferase reporter assay validated that miR-27a targeted the 3-untranslated region (3-UTR) of FSTL1. Moreover, miR-27a caused a significant decrease of FSTL1. In addition, the expression of TLR4 and NFκB was inhibited by miR-27a but increased by FSTL1 overexpression. In conclusion, we found that miR-27a inhibited cell migration and invasion of RA-FLS by targeting FSTL1 and restraining the TLR4/NFκB pathway.

Analysis and Detection of Transmission Lines Based on Twin Reality

Dong Yang,Bolin Du,Yan Lu,Suxin Zhang,Chengjun Xu,Jian Zhang 한국멀티미디어학회 2023 The journal of multimedia information system Vol.10 No.1

Now life has been transformed and upgraded to the direction of intelligence, digitalization and informatization along with the advancement of scientific and technological information. The traditional detection of transmission lines is completed by the professional maintenance per-sonnel of the power grid through manual detection of lines. This method is inefficient and has a certain threat to the life safety of detection personnel. The development of deep learning and computational vision supplies a fresh idea for detection of transmission lines. Therefore, this paper analyzes the traditional Faster R-CNN based on twin reality scene modeling technology. Then aiming at the shortcomings of traditional algorithms, the Cascade R-CNN algorithm is proposed to complete the detection of transmission lines based on twin reality system. It com-pares the accuracy and other indicators of Cascade R-CNN and Faster R-CNN algorithms. The effectiveness of two algorithms for analysis and detection of transmission lines is verified through experiments. Finally, the results indicates that the system using Cascade R-CNN has higher prediction accuracy, and has better practicability for analysis and detection of transmission lines.

Difference of Volatile Compounds According to Fatty Acid Contents of Three Different Coffee

Du-Min Jo,Dong-Gyu Yoo,Ha-Min Ji,Hye-Ryang Park,Dong-Lee Hong,Yang-Bong Lee 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Preparation of Optically Active Poly(N-propargylamide) Gels and Their Application in Chiral Recognition

Dong Liu,Xiaoying Du,Jianping Deng,Wantai Yang 한국고분자학회 2011 Macromolecular Research Vol.19 No.7

Optically active gels based on helical N-propargylamide polymers were prepared using a one-pot twostep methodology: the pre-formation of helical N-propargylamide copolymer (defined as N=N-HP) and the preparation of gels bearing the helical copolymers. To prepare N=N-HP, N-propargylamide M1, which contained -N=Nfunctional group, and M2, which provided helical polymer chains for the resulting copolymers, underwent copolymerizations in the presence of (nbd)Rh^+B^-(C_6H_5)_4 as a catalyst. -N=N- groups in N=N-HP acted as an initiator to induce the free radical polymerization of styrene, yielding the designed gels. Trimethylol propane triacrylate (TMPTA) was used to improve the cross-linking degree of the gels. The obtained gels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and circular dichroism (CD) spectroscopy measurements. The gels showed high optically activity, resulting from the helical structures of the N-propargylamide polymer chains. Preferential adsorption towards (R)-(+)-1-phenylethylamine in the two enantiomers was observed in the obtained gels, demonstrating the potentials of such gels in chiral recognition and chiral resolution.