인공간 시스템 적용을 위한 돼지 간세포의 대량 분리 및 배양

정유정,이혁준,고영택,김상범,김성훈,최석호,이남준,장성환,양은란,서경석,이윤신,이건욱 대한간학회 2002 Clinical and Molecular Hepatology(대한간학회지) Vol.8 No.3

목적: 전격성 간부전 환자의 유일한 치료법인 간 이식술은 공여간의 부족으로 제한된 환자에게서만 시술이 가능하다. 이러한 이유로 여러 가지 간기능 보조 장치가 개발 중인데 이중 체외 순환형 인공간 시스템 연구가 가장 활발히 진행되고 있다. 인공간 시스템의 연구에 있어서는 간 고유의 기능을 장기간 유지할 수 있는 간세포의 분리, 배양법이 집중적으로 연구되고 있다. 이에 본 연구진은 돼지에서 간세포 분리 및 배양 방법을 확립하고 단층 배양과 구 상체 배양의 기능을 비교 분석하고자 하였다. 방법: 10kg내외의 암퇘지에서 전간을 적출한 후 2단계 효 소관류 분리 방법에 의해서 간세포를 분리한 후 생존율을 검사하고 단층배양과 구상체 배양을 시도하였다. 암모니아 농도와 요소 농도를 측정해서 배양된 간세포의 기능을 규명하였다. 결과: 총 23마리 돼지 실험 결과 86.84± 8.04%의 생존율과 7.82± 5.40×109개의 세포 수를 얻었고, 단층 배양과 구상 체 배양 결과 암모니아 제거율과 요소 생성능에 있어서 구상체 배양이 단층 배양에 비해 우수하였음을 확인할 수 있었다. 결론: 본 연구를 통해 대량의 돼지 간세포 분리법을 확립하였고, 배양한 간세포는 기능상으로 안정된 세포로 인공간에 적용 가능하였다. 하지만 이상적인 인공간의 세포 충진 형태인 구 상체 간세포를 적용하기 위한 더욱 다양한 시도가 요구되리라 사료된다. Background/Aims: Acute hepatic failure is a serious problem. Its mortality reaches up to 80%. Only liver transplantation has been accepted as a definite treatment for patients with hepatic failure but shortage of donor organs is the main obstacle of this approach. A possible solution to this problem is a bioartificial liver system, perfusion of patients blood to isolated hepatocyte. In this study, we performed the isolation and culture of pig hepatocyte in large scale for the application of bioartificial liver system. Methods: Hepatocyte isolation was performed by two-step collagenase method via portal vein perfusion in 10kg female pigs. After that, we compared the functional differences of the spheroid culture to the monolayer culture of hepatocyte. The viability and the function of hepatocyte were assessed using trypan-blue exclusion test and the measurement of the rate of ureagenesis and ammonia removal. Results: The average viability and yield of hepatocyte were 86.8±8.0 % and 7.8±5.4×109, respectively. The spheroid culture was superior to the monolayer culture in functional aspect of hepatocyte, and their differences, especially for ammonia removal, were more apparent in parallel with culture time. Conclusions: For hepatocyte isolation, we obtained sufficient viability and yield of hepatocyte for clinical usage of bioartificial liver system. The function of hepatocyte seems to be better in the spheroid culture than in the monolayer culture. Further studies are needed for application of bioartificial liver system in clinical setting. (Korean J Hepatol 2002;8:249-255)

Nicotine exacerbates tacrolimus-induced renal injury by programmed cell death

( Yu Ji Jiang ),( Sheng Cui ),( Kang Luo ),( Jun Ding ),( Qi Yan Nan ),( Shang Guo Piao ),( Mei Ying Xuan ),( Hai Lan Zheng ),( Yong Jie Jin ),( Ji Zhe Jin ),( Jung Pyo Lee ),( Byung Ha Chung ),( Bum 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.6

Background/Aims: Cigarette smoking is an important modifiable risk factor in kidney disease progression. However, the underlying mechanisms for this are lacking. This study aimed to assess whether nicotine (NIC), a major toxic component of cigarette smoking, would exacerbates tacrolimus (TAC)-induced renal in-jury. Methods: Sprague-Dawley rats were treated daily with NIC, TAC, or both drugs for 4 weeks. The influence of NIC on TAC-caused renal injury was examined via renal function, histopathology, oxidative stress, mitochondria, endoplasmic reticulum (ER) stress, and programmed cell death (apoptosis and autophagy). Results: Both NIC and TAC significantly impaired renal function and histopathology, while combined NIC and TAC treatment aggravated these parameters beyond the effects of either alone. Increased oxidative stress, ER stress, mitochondrial dysfunction, proinflammatory and profibrotic cytokine expressions, and programmed cell death from either NIC or TAC were also aggravated by the two combined. Conclusions: Our observations suggest that NIC exacerbates chronic TAC nephrotoxicity, implying that smoking cessation may be beneficial for transplant smokers taking TAC.

Effects of post anneal for the INZO films prepared by ultrasonic spray pyrolysis

Lan, Wen-How,Li, Yue-Lin,Chung, Yu-Chieh,Yu, Cheng-Chang,Chou, Yi-Chun,Wu, Yi-Da,Huang, Kai-Feng,Chen, Lung-Chien Techno-Press 2014 Advances in nano research Vol.2 No.4

Indium-nitrogen co-doped zinc oxide thin films (INZO) were prepared on glass substrates in the atmosphere by ultrasonic spray pyrolysis. The aqueous solution of zinc acetate, ammonium acetate and different indium sources: indium (III) chloride and indium (III) nitrate were used as the precursors. After film deposition, different anneal temperature treatment as 350, 450, $550^{\circ}C$ were applied. Electrical properties as concentration and mobility were characterized by Hall measurement. The surface morphology and crystalline quality were characterized by SEM and XRD. With the activation energy analysis for both films, the concentration variation of the films at different heat treatment temperature was realized. Donors correspond to zinc related states dominate the conduction mechanism for these INZO films after $550^{\circ}C$ high temperature heat treatment process.

Seismic damage detection of a reinforced concrete structure by finite element model updating

Yu, Eunjong,Chung, Lan Techno-Press 2012 Smart Structures and Systems, An International Jou Vol.9 No.3

Finite element (FE) model updating is a useful tool for global damage detection technique, which identifies the damage of the structure using measured vibration data. This paper presents the application of a finite element model updating method to detect the damage of a small-scale reinforced concrete building structure using measured acceleration data from shaking table tests. An iterative FE model updating strategy using the least-squares solution based on sensitivity of frequency response functions and natural frequencies was provided. In addition, a side constraint to mitigate numerical difficulties associated with ill-conditioning was described. The test structure was subjected to six El Centro 1942 ground motion histories with different Peak Ground Accelerations (PGA) ranging from 0.06 g to 0.5 g, and analytical models corresponding to each stage of the shaking were obtained using the model updating method. Flexural stiffness values of the structural members were chosen as the updating parameters. In model updating at each stage of shaking, the initial values of the parameter were set to those obtained from the previous stage. Severity of damage at each stage of shaking was determined from the change of the updated stiffness values. Results indicated that larger reductions in stiffness values occurred at the slab members than at the wall members, and this was consistent with the observed damage pattern of the test structure.

한우 Band 3 및 CHS 유전병의 분자유전학적 기초연구

정행진,유성란,상병찬,이준헌 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.1

Many bovine genetic diseases are currently unidentified in Korea because of the relatively low monitoring systems in the livestock farms. The molecular detection system using PCR-RFLP of two genetic diseases, namely Band 3 (Erythrocyte Membrane Protein Band Ⅲ) and CHS (Chediak-Higashi Syndrome), have been identified in Japan and used for screening large number of cattle whether each individual has the genetic disease or not. Using the 22 unrelated Korean cattle (Hanwoo) individuals, molecular detection system based on PCR-RFLP have been investigated, which can be distinguishable carriers for the genetic diseases. Even though we could not found the causative mutations for two genetic diseases, the PCR-RFLP techniques used in this study are very valuable for the screening the genetics diseases in Korean cattle, especially for the proven or candidate bulls.

다양한 결손부를 가지는 휨지배 구조벽체의 구조성능 검증

유현진(Yu, Hyun Jin),강수민(Kang, Su Min),박홍근(Park, Hong-Gun),정란(Chung, Lan) 한국콘크리트학회 2018 한국콘크리트학회 학술대회 논문집 Vol.30 No.1

하수처리 : 막 결합형 생물 반응기에서 생물막 특성

김옥란 ( Yu Lan Jin ),김희윤 ( Hee Yoon Kim ),이우녕 ( Woo Nyoung Lee ),이정학 ( Chung Hak Lee ) 대한상하수도학회 2004 공동추계 학술발표회 논문집 Vol.2004 No.-

The development of Taiwan Fracture Liaison Service network

Lo-Yu Chang,Keh-Sung Tsai,Jen-Kuei Peng,Chung-Hwan Chen,Gau-Tyan Lin,Chin-Hsueh Lin,Shih-Te Tu,I-Chieh Mao,Yih-Lan Gau,Hsusan-Chih Liu,Chi-Chien Niu,Min-Hong Hsieh,Jui-Teng Chien,Wei-Chieh Hung,Rong-S 대한골다공증학회 2018 Osteoporosis and Sarcopenia Vol.4 No.2

Osteoporosis and its associated fragility fractures are becoming a severe burden in the healthcare system globally. In the Asian-Pacific (AP) region, the rapidly increasing in aging population is the main reason accounting for the burden. Moreover, the paucity of quality care for osteoporosis continues to be an ongoing challenge. The Fracture Liaison Service (FLS) is a program promoted by International Osteoporosis Foundation (IOF) with a goal to improve quality of postfracture care and prevention of secondary fractures. In this review article, we would like to introduce the Taiwan FLS network. The first 2 programs were initiated in 2014 at the National Taiwan University Hospital and its affiliated Bei-Hu branch. Since then, the Taiwan FLS program has continued to grow exponentially. Through FLS workshops promoted by the Taiwanese Osteoporosis Association (TOA), program mentors have been able to share their valuable knowledge and clinical experience in order to promote establishments of additional programs. With 22 FLS sites including 11 successfully accredited on the best practice map, Taiwan remains as one of the highest FLS coverage countries in the AP region, and was also granted the IOF Best Secondary Fracture Prevention Promotion award in 2017. Despite challenges faced by the TOA, we strive to promote more FLS sites in Taiwan with a main goal of ameliorating further health burden in managing osteoporotic patients.

하수처리 : 막 결합형 생물 반응기에서 생물막 특성

김옥란 ( Yu Lan Jin ),김희윤 ( Hee Yoon Kim ),이우녕 ( Woo Nyoung Lee ),이정학 ( Chung Hak Lee ) 한국물환경학회 ( 구 한국수질보전학회 ) 2004 공동 추계학술발표회 Vol.2004 No.-

댕댕이나무 줄기 추출물의 항산화 및 항암활성

Woo Jun Chung,Chan Ok Lee,Ju Hee Park,Eun Yeong Choi,Tae Kyoung Kim,So Hee Park,Nguyen Thi Lan Anh,Yong Soo Kwon,Chang Yeon Yu,Myong Jo Kim 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10

Background : Haskap berries commonly refer to fruits of Lonicera caerulea L., recognized by the Japanese aborigines as the “The elixir of life.”. Due to their recent arrival on the North American market, haskap berries have not yet been positioned among other berries and compared in terms of their phytochemical content. And haskap berries have higher ascorbic acid and anthocyanin content than other berries known for their health-promoting benefits, such as blueberries. However, no study has reported on the antioxidant and anti-cancer activity of Lonicera caerulea stem. The purpose of this study is to present the current research on the chemical content, antioxidant and anti-cancer activities of Lonicera caerulea stem. Methods and Results : The stem of Lonicera caerulea L. ware dried in the shade at room temperature and extracted with 100% methanol. The extract was suspended in deionized water and partitioned sequentially with n-hexane, chloroform, ethyl-acetate and butanol (water saturated BuOH) fractions. Antioxidant activities were measured by determination of antioxidants, DPPH (2,2-diphenyl-1-picrylhydrazyl). Cell viability was determined by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. All cell lines were purchased from the Korean Cell Line Bank (Seoul, Korea). All results were performed with three replications were processed statistically. By DPPH assay, the Lonicera caerulea L. the highest activity was obtained from the ethyl-acetate fraction (IC50=15.46 ㎍/㎖). By MTT assay, the chloroform fraction showed a significant growth inhibiting effect on MCF-7 (Human breast cancer, IC50=225.91 ㎍/㎖), COLO 205 (Human colon cancer, IC50=179.55 ㎍/㎖), but on AGS (Human stomach cancer) and other fractions it did not show effect. Conclusion : We demonstrated that Lonicera caerulea L. stem extract and fractions has antioxidant and antiproliferation activity in vitro. Further studies should identify the active constituents in Lonicera caerulea L stem to evaluate the potential in vitro antioxidant and antiproliferation activities of the extract.