Sludge를 이용한 자기 연마재 개발

김희남,윤여권,김상백,최희성,안효종 한국안전학회 2004 한국안전학회지 Vol.19 No.2

The magnetic polishing is the usefiil method to finish using magnetic power of magnet. This method is one of precision polishing techniques and has an aim of the clean technology using for the pure of gas and inside of the clean pipe for transportation. The magnetic abrasive polishing method is not so common for machine that it is not spreaded widely. There are rarely researcher in this field because of non-effectiveness of magnetic abrasive. Therefore, in this paper deals with development of the magnetic abrasive using sludge. In this development, abrasive grain WA and GC used to resin bond fabricated low temperature. And magnetic material was fabricated from the sludge which were crused into 200 mesh and average diameter 01.2mm ball type. The XRD analysis result show that only WA and GC abrasive and sludge crystal peaks detected which explains resin bond was not any more chemical reaction. From SEM analysis it is found that WA and GC abrasive and sludge were strong bonding with each other by bond.

6-(3,4-디클로로페닐)아미노-7-클로로-5,8-퀴놀린디온의 항진균작용 및 안전성 평가

윤여표,김동현,이병무,허문영,정해문,강혜영,최정아,김도희,유충규 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

6-(3.4-Dichlorophenyl)amino-7-chloro-5.8-quinolinedione (RCK50) was tested for antifungal activities in mice systemically infected with Candida albicans. The therapeutic potential of RCK50 was also assessed in comparison with ketoconazole. RCK50 had ED_50 0.22±0.01 ㎎/㎏. Ketoconazole as a positive control had ED_50 6.00±1.70 ㎎/㎏. Intraperitoneally administered RCK50 at the ED_50 for 7 days and 14 days reduced Candida albicans colony count in the kidneys and liver. And administered RCK50 at the ED_50 for 14 days improved survival rates. The genotoxicities of RCK50 had been evaluated. RCK50 was negative in Ames test with Salmonella typhimurium and chromosomal aberration test in CHL cells. RCK50 did not show any clastogenic effect in mouse peripheral blood and was negative in mouse micronucleus assay. These results indicate that RCK50 has no genotoxic potential under these experimental conditions. Acute oral toxicity studies of RCK50 were carried out in ICR mice of both sexes. RCK50 did not show acute oral toxicities and LD_50 values were over 2.850 ㎎/㎏ in ICR mice.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

소나무 및 편백나무 수용성 정유를 이용한 Trichoderma spp.의 생장억제 활성

여희동 ( Hee Dong Yeo ),정지영 ( Ji Young Jung ),남정빈 ( Jung Bin Nam ),김지운 ( Ji Woon Kim ),김희규 ( Hee Kyu Kim ),최명석 ( Myung Suk Choi ),양재경 ( Jae Kyung Yang ),Glen Alm,Danny Lee Rinker 韓國木材工學會 2009 목재공학 Vol.37 No.6

신갈나무로부터 유래된 추출물의 Botrytis cinerea 균주에 대한 항균활성

여희동 ( Hee Dong Yeo ),이형철 ( Hyung Chul Lee ),임부국 ( Bu Kug Lim ),김희규 ( Hee Kyu Kim ),최명석 ( Myung Suk Choi ),양재경 ( Jae Kyung Yang ) 한국목재공학회 2008 목재공학 Vol.36 No.1

목질자원으로부터 Botrytis속 곰팡이병에 대하여 항균활성을 나타내는 추출물을 탐색하고자 본 연구는 시도되었다. 신갈나무를 고압 전처리하고 이로부터 열수, 메탄올, 에탄올을 이용하여 목질 추출물을 획득하였다. Botrytis속 곰팡이 3종은 20℃와 25℃의 생장온도에서 최적 생장을 하였으며 pH 5.0과 7.0에서 가장 양호한 균사 생장을 나타냈다. 열수 추출물이 Botrytis속에 대하여 가장 우수한 항균활성을 나타내는 것을 확인하였다. 열수추출물로부터 항균물질을 분리하기 위하여 n-hexane : chloroform : ethyl acetate : formic acid (12 : 17 : 8 : 0.2, v/v/v/v)를 용제로 하는 silica-gel column chromatography와 TLC를 사용하였으며, 3종 추출물들은 HPLC 분석을 통하여 화합물을 확인하였으며 UV Spectrophotometer를 이용하여 열수 추출물로부터 6개의 fraction group을 분리 하였다. 6개의 fraction group의 항균활성 test결과, fraction group Ⅰ과 Ⅱ는 Botrytis cinerea에 대하여 가장 높은 항균활성을 나타냈다. Fraction group의 HPLC 분석결과, 3개의 유효물질을 확인하였으며 이 3가지 물질이 항균활성에 영향을 미친다고 추측된다. The aim of this study was to attempt the efficacy of antifungal activity of the wood extracts against Botrytis grey mold. Wood chip derived from Quercus mongolica was obtain from steam explosion process and extracted by hot water and methanol and ethanol. The conidial germination was maximum growth with the application temperature for 20 and 25℃. In pH test, we observed the maximum growth in pH 5.0 and 7.0. Antifungal activity was the best in the hot water extractives against Botrytis cinerea. The separation of the antifungal substances was performed using a silica-gel column (n-hexane : chloroform : ethyl acetate : formic acid = 12 : 17 : 8 : 0.2, v/v/v/v), TLC and UV-Spectrophotometer, and isolated 6 fraction group. The result of antifungal activity in 6 fraction group, fraction group Ⅰ and fraction group Ⅱ were the highest antifungal activity against grey mold with the present study. Three peaks in fraction group Ⅰ and Ⅱ were detected by HPLC and this compounds were suppose to effective of antifungal activity.

Anticancer Effects of Kimchi Inoculated with Leuconostoc mesenteroides in the Mouse Model of Colitis-Associated Colorectal Cancer

Kyung-Hee Lee,Hyun Ah Lee,Cui Meizi,Hee-Young Kim,Ji-Kang Jeong,Ji-Hee Hwang,Kyu-Young Yeo,Byung-Hee Ryu,Jung-Ho Choi,Kun-Young Park 한국식품영양과학회 2014 한국식품영양과학회 학술대회발표집 Vol.2014 No.10

장애인 공동생활가정 종사자의 업무환경이 직무만족에 미치는 영향

석여희(Yeo-Hee Seok),최희철(Hee-Chul Choi) 한국콘텐츠학회 2016 한국콘텐츠학회논문지 Vol.16 No.1

본 연구는 장애인그룹홈 종사자 148명을 대상으로 종사자의 업무환경이 직무만족에 어떠한 영향을 미치는지 분석한 것이다. 연구결과, 종사자들은 직장의 업무환경의 6개 하위영역 중 도전과 역할모호성에 대해서는 3.08점과 3.61점으로 보통 이상의 수준에서 인식을 하고 있었으나, 편안함, 금전적 보상, 역할갈등, 업무량에 대해서는 평균 1.80점에서 2.73점 사이로 보통 이하의 수준으로 상대적 취약성을 보였다. 다중회귀분석 결과 도전과 역할모호성이 유의미한 영향을 미치는 것으로 나타났다. 이러한 결과는 업무환경에서는 사회재활교사의 업무기준을 명시하고 다양한 방법으로 그룹홈 지원체계를 강화하여 종사자들의 자기개발과 도전의 기회를 높이고, 문제해결을 위해 필요한 지원이나 지지를 높여나가야 함을 제언하였다. This study analyzed how work environment of staff in group homes on their job satisfaction targeting 148 persons. As a result, this study found that the staff recognize challenge and role ambiguity at the level which is above level ranging from 3.08 to 3.61 points with respect to 6 subdomains of work environment, but showed a relative vulnerability regarding comfortability, monetary reward, role conflict and workload as below average ranging from 1.80 to 2.73 points. Results of multiple regression analysis show that challenge and role ambiguity exert a significant influence on their job satisfaction. The above results suggest that it is necessary to provide staff with an opportunity for self-development and challenge by specifying service standards for social rehabilitation teachers in work environment and strengthening group home support system in various ways, enhancing support or advocacy necessary for solving problems.

띠철근의 갈고리부분 정착 길이에 따른 철근콘크리트 기둥의 내진거동

최연희(Choi, Youn Hee),이여경(Lee, Yeo Kyeong),김희선(Kim, Hee Sun),신영수(Shin, Yeong Soo) 한국콘크리트학회 2019 한국콘크리트학회 학술대회 논문집 Vol.31 No.2

Life Science : 4-O-Methylhonokiol Attenuated Memory Impairment Through Modulation of Oxidative Damage of Enzymes Involving Amyloid-β Generation and Accumulation in a Mouse Model of Alzheimer`s Disease

( Im Seop Choi ),( Young Jung Lee ),( Dong Young Choi ),( Yong Kyung Lee ),( Yeun Hee Lee ),( Ki Ho Kim ),( Young Heui Kim ),( Young Ho Jeon ),( Eun Hee Kim ),( Sang Bae Han ),( Jae Kyung Jung ),( Yeo 영남대학교 약품개발연구소 2012 영남대학교 약품개발연구소 연구업적집 Vol.22 No.0

Accumulations of amyloid-β (Aβ) and oxidative damage are critical pathological mechanisms in the development of Alzheimer`s disease (AD). We previously found that 4-O-methylhonokiol, a compound extracted from Magnolia officinalis, improved memory dysfunction in Aβ-injected and presenilin 2 mutant mice through the reduction of accumulated Aβ. To investigate mechanisms of the reduced Aβ accumulation, we examined generation, degradation, efflux and aggregation of Aβ in Swedish AβPP AD model (AβPPsw) mice pre-treated with 4-O-methylhonokiol (1.0 mg/kg) for 3 months. 4-O-methylhonokiol treatment recovered memory impairment and prevented neuronal cell death. Thismemory improving activity was associated with 4-O-methylhonokiol-induced reduction of Aβ1-42 accumulation in the brains of AβPPsw mice. According to the reduction of Aβ1-42 accumulation, 4-O-methylhonkiol modulated oxidative damage sensitive enzymes. 4-O-methylhonkiol decreased expression and activity of brain beta-site AβPP cleaving enzyme (BACE1), but increased clearance of Aβ in the brain through an increase of expressions and activities of Aβ degradation enzymes; insulin degrading enzyme and neprilysin. 4-O-methylhonkiol also increased expression of Aβ transport molecule, low density lipoprotein receptor-related protein-1 in the brain and liver. 4-O-methylhonkiol decreased carbonyl protein and lipid peroxidation, but increased glutathione levels in the brains of AβPPsw mice suggesting that oxidative damage of protein and lipid is critical in the impairment of those enzyme activities. 4-O-methylhonokiol treatment also prevented neuronal cell death in the AβPPsw mousee brain through inactivation of caspase-3 and BAX. These

식물공장내 양액의 EC가 적겨자와 청경채의 생육 및 품질에 미치는 영향

이상규(Sang Gyu Lee),최장선(Chang Sun Choi),이준구(Jun Gu Lee),장윤아(Yoon Ah Jang),남춘우(Chun Woo Nam),여경환(Kyung-Hwan Yeo),이희주(Hee Ju Lee),엄영철(Young Chul Um) (사)한국생물환경조절학회 2012 생물환경조절학회지 Vol.21 No.4

최근 식물공장에 대한 연구가 활성화 되면서 식물공장내 인공광을 이용한 쌈채소 재배가 점점 늘어나고 있다. 특히 쌈채소를 좋아하는 우리나라에서는 그 소비량이 매년 증가하고 있으며, 토양재배보다는 수경재배로 생산한 엽채류를 좋아하기 때문에 식물공장 시설을 이용하여 수경재배로 쌈채소를 생산하면 그 효과가 크리라 생각된다. 따라서 본 실험은 식물공장내에서 쌈채소로 많이 이용되고 있는 적겨자와 청경채를 대상으로 양액농도별 수량과 비타민 함량의 차이를 분석하기 위하여 실시하였다. 그 결과, 적겨자와 청경채 모두 초장은 EC에 따른 유의성은 없었고, 엽수는 적겨자의 경우 EC 가 높아질수록 감소하는 경향을 보였으나 청경채의 경우는 유의성이 없었다. 청경채의 경우 EC가 높아질수록 엽면적이 현저하게 증가하였고, 생체중은 두 작물 모두 EC가 증가할수록 증가하는 경향을 보였다. 광합성 능력은 적겨자의 경우 뚜렷한 경향을 보이지 않았으나 청경채는 EC가 높을 때 높은 경향을 보였다. Ascorbic acid 함량은 적겨자의 경우에 EC가 낮을수록 높게 나왔고, 청경채의 경우에는 EC 2.0dS · m<SUP>?1</SUP> 처리구에서 가장 높았고, 1.5, 2.5dS · m<SUP>?1</SUP> 처리구 순이었다. 이상의 결과를 종합해 보면, 인공광 이용형 식물공장에서 적겨자와 청경채를 생산하고자 할 때에는 양액농도를 2.0~2.5dS · m<SUP>?1</SUP>로 관리하는 것이 생체중과 Ascorbic acid 함량을 고려해볼 때 가장 적절한 양액농도 관리 방법이라고 생각된다. Recently, researches related to plant factory system has been activated and production of Ssamvegetables using artificial lighting has been increasing. In South Korea, Ssam-vegetables are very popular and the consumption is increasing every year. Because leaf vegetables cultivated under hydroponic systems are more preferable rather than those cultivated by soil culture in Korea, the plant factory system would be more effective in production of Ssam-vegetables. Therefore, this study was carried out in order to analyze the yield and vitamin C contents in red mustard (Brassica juncea L.) and pak-choi (Brassica campestris var. chinensis), which are used a lot for the Ssam-vegetables in South Korea, as influenced by different concentrations of the nutrient solution in a plant factory system. As a results, there was no significant differences in the plant height among the treatment of EC in the nutrient solution, but for red mustard plants, the number of leaves tended to decrease in the treatment with higher EC. Leaf area of pak-choi plants was significantly increased in the higher EC, while the fresh weight had a tendency to increase along with increasing EC in the nutrient solution for both crops. The photosynthetic rates did not show a distinct tendency by EC levels for red mustard plants, but for pak-choi plants, it tended to be higher at the high EC. The contents of ascorbic acid in leaves were higher with decreasing EC concentration in the nutrient solution for red mustard plants, while the content was the highest at EC 2.0 dS m?1 for pak-choi plants. In summary, considering the marketable yields and vitamin C at different nutrient concentrations in a plant factory, the optimal concentration for red mustard and pak-choi plants was thought to be EC 2.0~2.5 dS m<SUP>?1</SUP>.