초등학교 아동의 세균학적 우식활성검사 결과와 우식경험도와의 상관관계에 관한 연구

조민정,김미형 광주보건대학 1999 論文集 Vol.24 No.-

Author investigated 42 children's caries experience who were in 1st grade of elementary school and performed Alban's test, cariescreen SM test and cariostat test for the purpose of obtaining correlation between the bacteriologic caries activity test and caries experience. Estimated correlation coefficient showed following results. 1.-Showed a very high correlation (P<0.01) between Alban's test result and dft index -Showed a significant correlation (P<0.05) between Alban's test result and fdt index 2.-Showed a significant correlation (P<0.05) between dft index and the results of cariescreen SM test and cariostat test -Showed a very high correlation (P<0.01) between sdt index and above-mentioned two tests 3. Showed a significant correlation (P<0.05) only between caries experience of permanent teeth and Cariostat test 4. Among above-mentioned three tests, Alban's test results of 48 hours and that of cariescreen SM test marked a significant correlation (P<0.05) with each other.

대학생의 우식활성 검사결과와 치아우식경험도와의 상관성에 대한 연구

조민정,김미형 광주보건대학 2000 論文集 Vol.25 No.-

Authors examined 55 college girl students' dental caries experience and performed Alban's test/Cariostat test/salivary flow rate test/ oral glucose clearance time test in order to obtain correlation between caries activity test and dental caries experience. Estimated correlation coefficients showed the following results. 1. showed a very high correlation(P<0.01) between Alban's test result and DMFT rate/FT index/DMFT index. 2. showed no correlation between dental caries experience and Cariostat test result/oral glucose clearance time test result. 3. showed a significant correlation between the result of salivary flow rate test and those of DMFT rate/DT index/DMFT index. 4. showed no correlation among the results of caries activity tests.

영아에서 수포 및 농포성 병변을 보인 과면역글로블린 E 증후군 1예

조재훈,김미연,박영민,김형옥 대한피부과학회 2004 大韓皮膚科學會誌 Vol.42 No.1

The hyper-IgE syndrome is characterized by marked elevated levels of IgE, recurrent cutaneous and systemic staphylococcal infections, peripheral eosinophilia and defective neutrophil chemotaxis. The characteristic cutaneous findings of hyper-IgE syndrome have been reported as an eczematous or atopic dermatitis-like eruption with multiple skin abscesses, whereas the initial presentation of hyper-IgE syndrome in infancy as a papulopustular or vesicular eruption. We report a case of hyper-IgE syndrome featuring a vesiculopustular eruption in infancy. A 15-month-old baby presented with recurrent vesiculopustules on the face and hands. The biopsy specimen revealed eosinophilic spongiotic dermatitis with an eosinophil-predominant perivascular lymphocytic infiltration. These findings might lead to an earlier recognition of hyper-IgE syndrome and an institution of appropriate treatment. (Korean J Dermatol 2004;42(1):108~110)

지루각화증과 유사한 임상양상을 보인 색소성 Bowen 병 1예

조유경,김미연,박영민,김형옥,안성열 대한피부과학회 2004 大韓皮膚科學會誌 Vol.42 No.3

Pigmented Bowen's disease is characterized by microscopically increased melanin pigment in the epidermis and/or papillary dermis in addition to the typical findings of Bowen's disease. Pigmented Bowen's disease is often confused clinically with pigmented lesions, such as seborrheic keratosis, melanocytic nevus, pigmented basal cell cacinoma and superficial spreading melanoma. A 74-year-old man presented with a black hyperkeratotic nodule ovrlying a solitary dark brownish patch on the scalp, this was clinically diagnosed as seborrheic keratosis. Histopathologic examination revealed pigmented Bowen's disease. We, herein, report an unusual case of pigmented Bowen's disease, which is the first reported case to have occurred on the scalp and to clinically resemble seborreic keratosis. (Korean J Dermatol 2004;42(3):381~383)

Mycobacterium abscessus에 의한 피부감염증 1예

조재훈,김미연,박영민,김형옥 대한피부과학회 2004 大韓皮膚科學會誌 Vol.42 No.4

We report a case of cutaneous Mycobacterium abscessus infection in a 30-year-old woman who had two, tender, erythematous subcutaneous nodules on the upper extremitiy. A skin biopsy specimen revealed a neutrophilic and histiocytic lobular, with septal panniculitis as well as some acid-fast bacilli in the dermis. The culture from a tissue specimen on Ogawa media for 7 days, yielded reaction. The patient was treated with incision and drainage, followed by administration of proper antibiotics. After 2 months the skin lesions were resolved without recurrence. (Korean J Dermatol 2004;42(4):512~515)

초등학교 아동의 소구치에 나타나는 교합면 이상결절의 발현에 관한 연구

김미형,조민정 광주보건대학 2000 論文集 Vol.25 No.-

The purpose of this study was to obtain the basic data of the developmental dental anomaly , dens evaginatus, of occlusal surface on maxillary and mandibular premolars. For this study, dental examinations had been taken for 900 elementary schoolchildren consisted of 11~13 year old 450 boys and 450 girls who were living in Damyang-gun and Kwangju city, Korea. Early recognition of this anomaly and timely treatment of dens eveginatus could preserve the pulpal vitality of these teeth. The results were as follows : 1. The comparision of persons with dens evaginatus on premolars : The mandibular premolars were more than maxillary premolars in male and female(P<0.05). 2. Reasons for failure to identify dens evaginatus on premolars were sound teeth, dental caries and filling teeth, thus maxillary sound teeth were more than mandibular teeth. 3. The number of dens evaginatus on premolars was not found(71.33%), one tooth(19.11%), two teeth(6.00%), three teeth(2.67%) and above four teeth(0.89%), but significant difference was not found in male and female(P>0.05). 4. Appearance of dens evaginatus on maxillary premolars: The 2nd premolars were more than the 1st premolars in male and female(P<0.05). 5. Appearance of dens evaginatus on mandibular premolars : The 2nd premolars were more than the 1st premolars in male and female(P<0.05).

급성 신우신염이 선행된 Escherichia coli 농흉 1예

차치운,조준형,김미진,오윤정,연재우,이성규,오미정,채지영,김수연 대한감염학회 2009 감염과 화학요법 Vol.41 No.5

We experienced a case of acute pyelonephritis which progressed to Escherichia coli bacteremia and later complicated by empyema in a 65-year-old female. She was successfully treated with intravenous antibiotic therapy and percutaneous drainage of empyema.

이황화탄소 중독자들의 노출중단 이후의 심박동수 변이

이상윤,조성일,백도명,변창범,김미정,박경근,임상혁,양길승,황창국,전형준 大韓産業醫學會 2006 대한직업환경의학회지 Vol.18 No.2

목적: 2000년도에 이황화탄소 중독증 환자들과 대조군의 심박동수 변이를 비교하여 이황화탄소 중독증 환자들의 심박동수 변이가 대조군에 비해 유의하게 낮다는 연구보고가 있었다. 당시의 연구가 이황화탄소 노출이 중단된 상태에서 이황화탄소 중독자들의 건강 문제를 연구했다는 점에 의의가 있으나 연구 결과를 해석하는데 있어 제한점들이 있었다. 이 연구는 2000년도에 수행하였던 연구의 제한점을 극복하고 이황화탄소 노출이 중단된 이후의 이황화탄소 중독자들의 심혈관계 상태를 심박동수 변이를 이용하여 평가해보고자 하였다. 방법: 이황화탄소 중독증으로 진단받은 환자 122명과 환자군의 연령대와 성별 빈도에 따라 무작위 추출한 112명을 대조군으로 선정하였다. 자기 기입식 설문지와 건강검진을 통해 연령, 성별, 신장, 체중, 흡연, 음주, 규칙적 운동, 질병력, 직업력, 흉부 방사선 촬영, 심전도 측정을 실시하였다. 연구 대상자들은 의자에 앉힌 상태에서 5분 동안 심박동수 변이를 측정하였다. 이 연구에서 측정한 심박동수 변이 지표들은 정상 RR간격의 표준편차(standard deviation of all norma-to-normal intervals, SDNN), 인접한 RR간격들의 차이를 제곱의 합의 평균의 제곱근(square root of the mean of the sum of squares of differences between adjacent normal-to-normal intervals, RMSSD). 저주파영역(low frequency power, LF: 0.04~0.15Hz), 고주파 영역 (high frequency power, HF:0.15~0.4Hz), 전체 주파수 강도(total power, TP), 저주파/고주파 비 (LF/HF ratio)이다. 결과: 단변량 분석에서 이황화탄소 중독자들의 모든 심박동수 변이 지표들이 대조군에 비하여 낮았다. 다중선형회귀분석에서 이황화탄소 중독은 RMSSD를 유의하게 감소시키는 것으로 나타났다.(P<0.05). 결론: 이 연구는 이황화탄소 노출이 중단된 상태에서도 이황화탄소 중독자들에게 심혈관계와 관련된 건강문제가 있을 수 있음을 시사한다. Objectives: A previous study conducted in 2000 measuring the heart rate variability (HRV) of carbon disulfide (CS_(2))-poisoned subjected suggested that their HRV was reduced after exposure cessation. However, the study was limited by the following procedural limitations: (1) only 71 CS_(2)-poisoned subjects participated, (2) no females participated, and (3) the CS_(2)-poisoned subjects were older than the controls. This study was therefore conducted to overcome these limitations of the earlier study. Methods: The study subjects comprised 122 retired workers with CS_(2) poisoning and the same number of age- and sex-matched controls. Information on individual age, sex, height, wight, smoking history, alcohol drinking, regular exercise, medical and occupational history, chest x-ray, and ECG recording of the two groups was collected though a self-administered questionnaire and a medical examination. Standard Deviation of NN intervals (SDNN), Root-Mean-Square of Successive Differences (RMSSD), Total Power (TP), Low Frequency (LF), High Frequency (HF), and LF/HF ratio were measured as HRV indices for 5 minutes in the sitting position. Results: Univariate analysis revealed that all HRV indices of CS_(2)-poisoned subjected were lower than those of the controls. Multiple linear regression analysis showed that CS_(2) poisoning had negative association with all HRV indices and that its association with RMSSD was statistically significant(P<0.05). Conclusions: This study suggests that CS_(2)-poisoned subjects continue to have reduced HRV, even though the exposure has ceased.

망막아세포종 세포주(Y-79)의 Arylalkylamine N-acetyltransferase 활성에 미치는 Dopamine의 영향

김석민,정미영,윤경식,진병관,백행운,백형환,조용호 慶熙大學校 1997 論文集 Vol.26 No.-

The arylalkylamine N-acetyltransferase(AA-NAT, EC 2.3.1.87) in vertebral pineal gland and retina is rate-limiting enzyme in the pathway of biosynthesis of melatonin. AA-NAT activities are remarkably increased during night with 10-150 folds. Neurohormone melatonin is thought to play roles in a broad range of physiological functions in human, primarily through effects on the biological clock in the suprachiasmatic nucleus of the hypothalamus, This rhythm reflects large changes in the activities of AA-NAT. So the important regulatory role of AA-NAT has made it central interest in research on melatonin biochemistry and neurochemical signal transduction. The neurotransmitter that regulates the AA-NAT activities is norepinephrine, which acts through a cyclic AMP mechanism. The effect of dopamine on AA-NAT in human retinoblastoma Y-79 cells was studied. the obtained results are as follows; AA-NAT activities in Y-79 cells were increased by cyclic AMP, and dopamine inhibited the AA-NAT activities stimulated by forskolin. The pattern of inhibition of dopamine on the AA-NAT activities in Y-79 cells was time and concentration dependent. The results suggest that dopamine participates in the regulation of the AA-NAT activities through the inhibitory mechanism in the human pineal and retinal tissues.

Escherichia coli(M15)을 이용한 serotonin N-acetyltransferase 유전자의 발현

김용만,정미영,윤경식,진병관,백행운,조용호,백형환 慶熙大學校 1997 論文集 Vol.26 No.-

AA-NAT cDNA was obtained by RT-PCR technique from total RNA of rat sacrified at night(02:00am). pCRNAT was cloned using the pCRII vector with insertion of AA-NAT cDNA(about 1.4 kb) at EcoRI site. For the expression of the gene, pQECNAT was subcloned, in which the coding region of AA-NAT was inserted into expression vector pQE30 at BamHI and HindIII sites. According to the experimental results, Escherichia coli strain M15, transformed by the expession vector pQECNAT, was selected as a host to express AA-NAT gene with the induction of isopropyl β-D-thiogalactoside(IPTG). Optimal condition for the expression of AA-NAT gene was achieve from the experimental results, showing that the expression of the protein was inducible much 19.800 ± 2,200 dpm per ml of culture volume in 4 hours at the concentration of 2 mM IPTG. Partial purification through the affinity column(Ni-NTA agarose) binding to the continuous 6 histidine residues of protein resulted in 5 times more increase in the specific activity of AA-NAT than that of the homogenate of bacterial pellet. These experimental results will provide basic data in further study for the enzymatic kinetics and antibody production of AA-NAT.