백서에서 신 허혈성 손상에 미치는 칼슘 길항제의 효과

문철웅,정종훈,박천국,이승일,배학연,장경식,김만우,정춘해,홍순표,이병래,김호중 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Renal ischemia is one of the most common causes of acute renal failure. Four factors related to the pathogenesis of acute renal failure are vasoconstriction, decreased glomerular filtration rate, tubular back leak of filtrate, and intratubular obstruction. The cellular response to renal ischemic insults include decreased content of adenosine trihosphate, lipid peroxidation induced membrane degradation, alteration in cellular pH, and calcium or phospholipase induced mitochondrial dysfunction. Much attention has been given to the role of increased cellular calcium as a pathogenetic contributor to cell injury during ischemia. Author studied the protective effects of calcium antagonists on cellular injury during renal ischemia in rat. To investigate the protective role of these agents, author measured the amount of malondialdehyde(MDA) and the enzyme activities of free radical scarvengers-superoxide dismutase(SOD), catalase and glutathione peroxidase from non-pretreated group and calcium antagonists pretreated groups in control, ischemia and reflow subgroups. The results were summerized as follows: 1) The amount of MDA in non-pretreated group was higher in the reflow compared with the control(<p<0.01). But, in all pretreated groups, there was no statistically difference in the amount of MDA. 2) The SOD activity in non-pretreated group was lower in both the ischemia and the reflow compared with the control (P<0.05). But, in both verapamil and trifluoperazine-pretreated groups, there was no statistically difference in the SOD activity. 3) Both catalase and glutathione peroxidase activities in non-pretreated group were lower in both the ischemia and the reflow compared with the control (P<0.05). But in all pretreated groups, there was no statically difference in both catalase and glutathione peroxidase activities. These results suggest that free radical mediated ischemic injury by renal artery clamp in rat can be protected by intraperitoneal pretreatment with calcium antagonists. As trifluoperazine has a protective effect in renal ischemia, the calcium activated calmodulin dependent enzyme may play a role in renal ischemic injury.

기내배양에 의한 Panax속(屬) 종자의 발아 및 생장특성

정찬문 충북대학교 연초연구소 2006 煙草硏究 Vol.20 No.-

인삼속의 종간비교시험의 일환으로 고려인삼, 미국삼 그리고 죽절삼을 공시하여 이들의 종자 특성을 비교하였다. 그리고 기내에서 배배양 방법에 의해 발아율 및 생장을 검정하였다. 종자는 미국삼이 납작한 형태의 편형(扁形)이고 고려인삼이 납작하면서 둥근형태의 편구형(扁球形)이며 죽절삼이 둥근 형태의 구형(球形)이었다. 그리고 배생장 및 발아는 고려인삼과 양호하였고 미국삼과 죽절삼은 불량하였다. 그리고 환경에 따른 발아율은 기내배양, 생육상 그리고 포장의 순으로 양호하였으며 종간의 발아율은 고려인삼이 가장 양호하였다. 특히 배배양은 종자의 활성이 잘 나타나므로 기내배양에 의한 종자의 발아능 테스트를 위한 간이 검정법으로 이용할 수 있을 것으로 사료된다. This study was carried out to investigate the characteristics and growth of seed in genus of panax using Korean ginseng(Panax ginseng), American ginseng(Panax quinquefolium) and Jukjeal ginseng(Panax japonuicum) through field and in vitro examination. Seed have three morphological type that was observed flat in American ginseng, flat and globular in Korean ginseng and globular shape in Jukjeal ginseng. Korean ginseng was good growth of embryo and germination but American ginseng was not. As for rate of germination, Korean ginseng was the best compared American and Jukjeal ginseng. In culture condition, In vitro culture was good than other growth chamber and field. Using Embryo culture, It is possible to test activity of seed and capacity of germination as simple test method of seed.

고려인삼의 주아 발달 경로에 대하여

정찬문,임흥빈,이이,정열영,전병록 충북대학교 연초연구소 2009 煙草硏究 Vol.22 No.-

본 시험은 인삼의 채종종자의 조직을 관찰하여 잠아의 실체를 조사하였고 묘삼의 잠아특성을 산지별로 조사하여 다경이 되는 잠아의 발달경로를 알아보았다. 그리고 묘삼을 본포에 이식하여 3년생을 채굴하여 4년생이 될 주아의 생성경로를 조사하였다. 1. 묘삼의 주아는 발아종자의 경근천이부에서 피층조직이 융기하여 돔(dome)형태의 원잠아 또는 측잠아가 형성된 후 이들 조직이 분화하여 발달하였다. 2. 묘삼의 단뇌는 90%가 원잠아에서 유래하였고 쌍뇌는 원잠아와 2%정도의 측잠아1개가 발달되어 형성되었으며 3뇌는 원잠아와 측잠아 2개가 발달하여 형성되었다. 3. 본포에서 2년생과 3년생은 주아가 단뇌로 형성되어 있어 2경개체가 발생하지 않으나 4년생은 3년생에서 발달한 쌍뇌 이상의 주아가 있어 2경개체이상이 관찰되는 최초의 연생이었다. 4. 4년생의 주아는 종자에서 형성된 원잠아, 측잠아와 2-3년생에서 뇌두부위에서 형성된 피층잠아에서 유래하는 것으로 고년생으로 갈수록 쌍뇌 또는 3뇌는 원잠아에 비해 피층잠아 유래의 주아가 많았다. We studied the substance of latent bud by surveying the embryo of harvested seed and developmental pathway of latent bud by analysing the characteristics of latent bud of local ginseng lines. We transplanted one year old ginseng seedlings in the field and harvested it out two years later. And then We investigated developmental pathway of the main bud which would be the shoot of ginseng in the fourth year. Main bud of seedling was formed from the region between root and shoot of germinating seed. Primary and axillary latent buds protruded in dome shape from cortex tissue and differentiated into main bud. Ninety percent of single main bud was derived from primary latent bud, twin main buds were derived from primary latent bud and one axillary bud, and triple main buds were derived from primary latent bud and two axillary latent buds. In the field, We could not find 2-stem plant from 2-3 years old plant, however, We found 2-stem plant from 4-year-o1d plant because twin main buds developed from 3-year-o1d plant. Therefore 2-stem plant was observed obviously from the plant at least 4 years old. Main buds of 4-year-old plant were formed from primary and axillary latent bud of seed and cortex latent bud of rhizome from 2-3 years old plant. In older plants, twin and triple main buds were derived more from cortex latent bud than primary latent bud.

고려인삼의 홍삼 품질평가요인 분석

정찬문,엄유리,김상민,이이,이문순 忠北大學校 農業科學硏究所 2012 農業科學硏究 Vol.28 No.2

This study was conducted to evaluate the elements affect on the quality of red ginseng. For that purpose, we surveyed the relationship between the red ginseng quality and the properties of material ginseng, processing methods, root year, root size, production location, and cultivation methods. Six-year old raw ginseng caused higher inside cavity than younger one did and larger sized ginseng increased outside cracking and inside cavity but decreased inside white than smaller sized one did. The production location did not affect the quality of red ginseng. The red ginseng processed with the ginseng grown on upland field showed lower outside cracking and inside cavity than the red ginseng processed with the ginseng grown on paddy field did. In terms of cultivation method, transplanting method showed lower inside cavity and inside white rates than direct sowing method did. In conclusion, ginseng cultivation location and cultivation method affected on the quality of the red ginseng more than the root age, root size and production location did.

인삼의 변종 간 교잡에 의한 종자특성

정찬문,신주식 충북대학교 연초연구소 2006 煙草硏究 Vol.20 No.-

고려인삼의 변종 자경종, 황숙종, 청경종 그리고 KG107을 교배모본으로 하여 정역간 교잡을 실시하여 이들의 결실률, 발아특성을 조사하였다. 인삼 변종의 전체 결실률 80.5-86.7 %로서 자경종이 가장 높았고 KG107이 가장 낮았다. 교배조합의 결실율은 자경종과 황숙종을 모본으로 하였을 경우 결실률은 50 % 이상으로 차이가 없으나 청경종과 KG107을 모본으로 하였을 경우 40 %이하로 교배조합 간에 차이가 많았다. 열매의 길이 및 무게의 경우 KG107이 불량하였고 변종의 종자 수는 2개 이상이 60 %이상이었다. 그러나 교배조합은 1립종자와 2립종자의 구성 비율이 교배조합에 따라 차이가 있었다. 발아율은 대체로 교배조합이 교배모본 70 %에 비해 높았으나 KG107의 교배조합은 발아율이 저조하였다. 따라서 변종 및 교배조합의 결실률, 종자품질 그리고 발아율은 모본과 교배조합에 따라 차이가 많았다. This study was conducted to investigate the rate of fertilization, characteristics of germination in variants of Panax ginseag(violet-stem, yellow-berry, green-stem and KG107) through reciprocal crossing. In rate of fertility, Mother plant was 80.5-86.7 %. Violet stem variant was good compared other variants but KG107 was not and the cross combination was over 50 %. Especially Violet-stem and yellow-berry variant as mother plant were not difference but green-stem variant and KG107 as mother plant was under 40%. Therefor rate of fertility in variants and cross combination had much difference. On the other hand, KG107 was bad fruit of length and weight. Number of seed of variant had double seed over 60 % but cross combination had difference in single and double seed according to cross combination. Rate of germination of mother plant and cross combination was higher than 70 % but some of cross combination was bad when was used mother and father plant as KG 107. In variants and cross combination, rate of fertility, quality of seed and rate of germination had difference according to among mother plant and cross combination.

수종 복합레진 내의 bis-GMA와 TEGDMA의 구성비가 레진 인레이 법에 의한 부가적 열처리식 복합레진의 표면 및 내부의 중합률 변화에 미치는 영향

정찬문,박성호 大韓齒科保存學會 1996 Restorative Dentistry & Endodontics Vol.21 No.2

인삼에 있어 변조 장뇌삼 판별에 대하여

정찬문,신주식,이이,장세영 충북대학교 연초연구소 2004 煙草硏究 Vol.19 No.-

시장에서 시판되고 있는 장뇌삼을 재료로 하여 변조 특성 및 이들 변조삼의 진위여부를 판별하고자 그 특성을 조사하였다. 장뇌삼의 변조는 뇌두와 뿌리부위를 주로 변조하는 것으로 나타났다. 뇌두부위는 경흔적수와 수근수를 늘리던가 아니면 뇌두를 장뇌로 변조하였고 뿌리 부위는 지근수 및 지근장을 늘리는 것으로 나타났다. 변조한 뇌두부위의 특징은 먼저 뇌두하단에 있는 동체의 중심주를 요(凹)모양으로 만들고 변조한 뇌두를 철(凸)모양으로 깍아 심지 박듯이 맞추는 것이었다. 또한 각기 다른 인삼의 뇌두를 교묘히 잇대어 장뇌로 만들었고 여기에 수근을 접착하여 오래된 진짜 산삼 형태와 유사하게 변조하였다. 그리고 뿌리부위는 접착면을 절편 자르듯 사선으로 잘라 접착면이 많게 하였고 후에 이들 접착면을 비벼 흔적을 없애는 수법이었다. 변조에 사용된 재료는 인삼 및 출처불명의 약초뿌리를 사용하였고 접착제는 공업용으로 접착성이 강한 것을 사용하였다. For identification of altering methods and distinction of altered long-rhizome ginseng, we studied the properties of long-rhizome ginseng which is being sold in market. Artificial long-rhizome ginsengs were made by modification of rhizomes and roots. Artificial long rhizomes were made by increasing the number of stem vestige root and rhizome root or by modification of rhizome to long-rhizome. Roots were modified by increasing of the number and the length of secondary roots. Artificial long rhizomes were made by carving the rhizome in concave shape and attaching convex shaped modified another rhizome. Those who make artificial ginseng made the altered long-rhizome ginseng looks like real long-aged wild ginseng by elongation of the rhizome by attaching many ginseng rhizomes and attaching of secondary roots. They cut the attaching surface obliquely for increased number of attaching point and removed the vestige by scrubbing the attaching region. They used ginseng, medicinal plant from doubtful origin, and industrial strong adhesives for making altered long-rhizome ginseng.

적외선 흡수 분광법 및 DPC를 이용한 치과용 수복재의 광중합 거동 분석

정찬문,김중곤,최준호,장두옥,김경남,김광만 대한치과기재학회 1998 대한치과재료학회지 Vol.25 No.1

Mechanical properties of compomers would be obtained mainly by photopolymerization by visible light irradiation. Photopolymerization behavior of two commercial compomers was investigated by FT-IR spectral analysis and differential photocalorimetry(DPC). The results obtained were as follows: 1. From FT-IR analysis, it was found that the compomer products were photocured mainly by radical polymerizations of multifunctional methacrylate monomers. Final conversion values of the polymerizations after exposure to visible light for 60 sec were 67% for Dyract and 59% for Compoglass. 2. Upon exposure the compomers exhibited autoacceleration and autodeceleration, which are usually observed in bulk polymerizations of multifunctional monomers. 3. Dyract showed higher rate of polymerization and conversion of double bond than Compoglass.

통기방식에 의한 고려인삼의 기관분화 특성

정찬문,신주식,이이,장병용 충북대학교 연초연구소 2004 煙草硏究 Vol.19 No.-

기내 배양환경 개선방안의 일환으로 강제통기방식을 이용한 치상배의 발아유형과 생장특성 등을 구명하였다. 강제 통기방식에 의한 배양용기는 기존의 삼각플라스크 배양에 비해 결로 현상이 적었을 뿐 치상절편의 기관분화 및 생장에는 효과가 없었다. 그러나 기내의 공기를 환기시키고 GA등 양액을 추가 공급하여 배양체의 생장을 도모할 수 있는 장점이 있는 반면 오염율은 높았다. 발아유형별 기관분화는 자엽 전개가 양호한 B type이 좋았으나 자엽이 전개하지 않은 A Type과 상배축이 전개한 C Type은 불량하였다. 배(embryo)의 부위별 callus 생장은 자엽과 경근이동부가 좋았고, 배를 전체배양 했을 때 callus 노화가 쉽게 일어났다. 절편체에서 shoot 분화는 자엽과 경근이동부가 양호하였다. For the improvement of in vitro incubation conditions, we analyzed the germination and growth of zygotic embryo explant using forced ventilation. Forced ventilation did not show significant difference in organ formation and growth of zygotic embryo explant compared to triangular flask except for lower dew formation. Ventilation and GA addition to the medium increased not only the growth rate but also the contamination rate. We tested the organ formation rate according to the germination type. B type, which has good cotyledon formation, showed higher organ formation rate, but A type, which has no cotyledon, and C type, which has hypercotyl, showed lower organ formation rate. We tested the callus growth rate according to the part of embryo. Calli from cotyledon and the region between root and stem showed higher growth rate. Calli induced from whole embryo showed early senescence. Cotyledon and the region with root and shoot showed good explant shoot formation.

냉간 압연용 작업롤의 제조 기술에 따른 특성

전언찬,김문경,김순경,이충엽 동아대학교 공과대학부설 생산기술연구소 1997 生産技術硏究所硏究論文集 Vol.2 No.1