말단비대증을 동반한 뇌하수체 선종들의 면역조직화학적 및 임상적 특성의 비교

이명식,김성훈,김광원,민용기,고은미,정재훈,김경아,안규정,김병준,김진석,이문규,김종현,서연림,나동규 대한내분비학회 1998 Endocrinology and metabolism Vol.13 No.3

Background: It assumed that plurihormonal pituitary adenomas in acromegaly, which were immunohistochemically stained with other pituitary hormones in addition to GH and prolactin, would be originated from poorly differentiated cells. Therefore, we speculated that they might have higher growth rates and worse prognosis than monohormonal adenomas. To verify this speculation, we analyzed the frequency of plurihormonal adenomas and compared the clinical parameters and radiological invasiveness between plurihormonal adenoma and GH-prolactin adenoma in acromegaly. Methods: We studied 38 patients with acromegaly (22 males and 16 females, mean age 40.7 years) who were underwent surgical removal of pituitary adenomas by TSA from January 1995 to February 1998. We performed immunohistochemical staining in these tumors using avidinbiotin peroxidase complex method. An adenoma was considered as immunoreactive when above 50 percents of tumor cells were stained with anti-hormonal antibodies. Invasiveness of tumors were evaluated by preoperative MRI findings on the basis of Hardys classification. Results: The frequencies of plurihormonal and GH-prolactin adenomas were 42% and 58%, respectively. Plurihormonal adenoma included an adenoma which was not stained with prolactin, but with GH and other hormones. Prolactin immunoreactivity was found in 97%(37/38) of the tumors. Immunoreactivities to FSH, ACTH, LH, and TSH were found in 37.8%, 13.1%, 2.6% and 2.7%, respectively. There were no significant differences in age, basal serum GH and IGF-1 concentrations between plurihormonal and GH-prolactin adenomas. There were also no significant differences in response to TRH & LH stimulation tests and somatostatin & bromocriptine suppression tests between two groups. There were no differences in radiological invasiveness between two groups (plurihormonal adenoma, grade I 2, grade II 3, grade III 7, grade IV 4; GH-prolactin adenoma, grade I 3, grade II 6, grade III 9, grade IV 4). Conclusion: Plurihormonal adenomas were 44% and immunoreactivity to prolactin was 97% in pituitary adenomas in acromegaly. There were no significant differences in clinical parameters and radiological invasiveness between plurihormonal and GH-prolactin adenomas in acromegaly (J Kor Soc Endocrinol 13:324-330, 1998).

Developing rice mutants having superior quality through gene editing technology

Ah-Rim Lee,Backki Kim,Hyunjin Park,Tae-ho Ham,Joohyun Lee,Soon-Wook Kwon 한국육종학회 2023 한국육종학회 공동학술발표집 Vol.2023 No.-

Screening for depression and anxiety disorder in children with headache

Lee, Sang Mi,Yoon, Jung-Rim,Yi, Yoon Young,Eom, Soyong,Lee, Joon Soo,Kim, Heung Dong,Cheon, Keun-Ah,Kang, Hoon-Chul The Korean Pediatric Society 2015 Clinical and Experimental Pediatrics (CEP) Vol.58 No.2

Purpose: The purpose of this study was to investigate the importance of initial screening tests for depression and anxiety disorders in children with headache. In addition, this study evaluated whether the Children's Depression Inventory (CDI) and Revised Children's Manifest Anxiety Scale (RCMAS) are suitable for screening symptoms of depression and anxiety. Methods: A retrospective chart review was conducted of 720 children aged 7-17 years who had visited a pediatric neurology clinic for headaches and were referred to a pediatric psychiatric clinic for psychiatric symptoms from January 2010 to December 2011. All patients completed the CDI and RCMAS. Among them, charts of patients with clinically significant total scores (cutoff>15) for psychiatric symptoms, as defined by the CDI and RCMAS scoring scales, were reviewed. Results: Nineteen patients had headaches and clinically significant total scores for psychiatric symptoms. The mean age at headache diagnosis was 11.7 years, and 57% were male. Mean duration of headache was 11.5 months. Two point eight percent of the patients were diagnosed with psychiatric disorders including major depression (1.7%), generalized anxiety disorder (1.1%), and bipolar disorder (0.1%). Four patients (0.6%) were diagnosed with attention deficit/hyperactivity disorder (ADHD). Total mean CDI and RCMAS scores of patients referred to the psychiatric clinic were 18.8 and 22.2, respectively. There was no correlation between CDI or RCMAS total scores and headache frequency, duration, or severity. Conclusion: We recommend that all patients with headache should be screened for depression and anxiety by CDI and RCMAS scores.

Treatment with bisphenol A and methoxychlor results in the growth of human breast cancer cells and alteration of the expression of cell cycle-related genes, cyclin D1 and p21, via an estrogen receptor-dependent signaling pathway.

Lee, Hye-Rim,Hwang, Kyung-A,Park, Min-Ah,Yi, Bo-Rim,Jeung, Eui-Bae,Choi, Kyung-Chul UNKNOWN 2012 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.29 No.5

<P>Various endocrine disrupting chemicals (EDCs) are exogenous compounds found in the environment and have the potential to interfere with the endocrine system and hormonal regulation. Among EDCs, bisphenol?A (BPA) and 1,1,1-trichloro-2,2-bis(4-methoxyphenol)-ethane [methoxychlor (MXC)] have estrogenic activity resulting in a variety of dysfunctions in the E2-mediated response by binding to estrogen receptors (ERs), causing human health problems such as abnormal reproduction and carcinogenesis. In this study, we investigated the effects of BPA and MXC on cell proliferation facilitated by ER signaling in human breast cancer cells. MCF-7 cells are known to be ERα-positive and to be a highly E2-responsive cancer cell line; these cells are, therefore, a useful in?vitro model for detecting estrogenic activity in response to EDCs. We evaluated cancer cell proliferation following BPA and MXC treatment using an MTT assay. We analyzed alterations in the expression of genes associated with the cell cycle in MCF-7 cells by semi-quantitative reverse-transcription PCR following treatment with BPA or MXC compared to EtOH. To determine whether BPA and MXC stimulate cancer cell growth though ER signaling, we co-treated the cells with agonists (propyl pyrazoletriol, PPT; and diarylpropionitrile, DPN) or an antagonist (ICI 182,780) of ER signaling and reduced ERα gene expression via siRNA in MCF-7 cells before treatment with EDCs. These studies confirmed the carcinogenicity of EDCs in?vitro. As a result, BPA and MXC induced the cancer cell proliferation by the upregulation of genes that promote the cell cycle and the downregulation of anti-proliferative genes, especially ones affecting the G1/S transition via ERα signaling. These collective results confirm the carcinogenicity of these EDCs in?vitro. Further studies are required to determine whether EDCs promote carcinogenesis in?vivo.</P>

A new multifunctional Schiff base as a fluorescence sensor for Al³⁺ and a colorimetric sensor for CN⁻ in aqueous media: an application to bioimaging

Lee, Seul Ah,You, Ga Rim,Choi, Ye Won,Jo, Hyun Yong,Kim, Ah Ram,Noh, Insup,Kim, Sung-Jin,Kim, Youngmee,Kim, Cheal The Royal Society of Chemistry 2014 Dalton Transactions Vol.43 No.18

<P>A multifunctional fluorescent and colorimetric receptor <B>1</B> ((<I>E</I>)-<I>N</I>′-((8-hydroxy-1,2,3,5,6,7-hexahydropyrido[3,2,1-<I>ij</I>]quinolin-9-yl)methylene)benzohydrazide) for the detection of both Al<SUP>3+</SUP> and CN<SUP>−</SUP> in aqueous solution has been developed. Receptor <B>1</B> exhibited an excellent selective fluorescence response toward Al<SUP>3+</SUP>. The sensitivity of the fluorescent based assay (0.193 μM) for Al<SUP>3+</SUP> is far below the limit in the World Health Organization (WHO) guidelines for drinking water (7.41 μM). In addition, receptor <B>1</B> showed an excellent detection ability in a wide pH range of 4–10 and also in living cells. Moreover, receptor <B>1</B> showed a highly selective colorimetric response to CN<SUP>−</SUP> by changing its color from colorless to yellow immediately without any interference from other anions.</P> <P>Graphic Abstract</P><P>A new multifunctional Schiff-base receptor <B>1</B> which exhibits an excellent cell-permeable fluorescence for Al<SUP>3+</SUP> and a color change for CN<SUP>−</SUP> in aqueous media has been prepared. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4dt00361f'> </P>

Cyp7a1 is continuously increased with disrupted Fxr-mediated feedback inhibition in hypercholesterolemic TALLYHO/Jng mice

Lee, Eun-Ah,Lee, Dong-In,Kim, Hee-Yoen,Ahn, Sung-Hoon,Seong, Hye-Rim,Jung, Won Hoon,Kim, Ki Young,Kim, Sungsub,Rhee, Sang Dal Elsevier 2018 Biochimica et biophysica acta, Molecular and cell Vol.1863 No.1

<P>TALLYHO/Jng (TH) mice reveal hypercholesterolemia at an early age before their plasma glucose levels have increased. The increased plasma cholesterol should be related to bile acids (BAs) metabolism, because cholesterol is the precursor of BAs and BAs change cholesterol metabolism in a feedback manner. We analyzed the BAs pool size, BAs composition, and expression levels of several proteins that have key roles in BAs synthesis, excretion, and reabsorption and compared them to those of C57BL/6 (B6) mice to study BAs metabolism in TH mice. TH mice exhibited an increased total BAs pool size, increased BAs content in the cecum feces, and an increased ratio of muricholic acid (MCA)/cholic acid (CA). The mRNA and protein levels of cholesterol 7 alpha-hydroxylase (Cyp7a1) and the ATP-binding cassette sub-family G member 5 (Abcg5) were elevated in the liver but not in the apical sodium bile acid transporter (Asbt) and organic solute transporters (Osts) in the ileum. These results indicate that synthesis and the excretion of BAs from the liver to the gallbladder might be elevated, but the reabsorption rate of BAs in the ileum might be reduced. The declined expression of fibroblast growth factor 15 (Fgf15) and fibroblast growth factor receptor 4 (Fgfr4) was respectively identified in the ileum and the liver, indicating the disrupted feedback inhibition of Cyp7al. Consequently, hypercholesterolemia in TH mice might increase the BAs amounts via the interrupted Fxr/Fgf15/Fgfr4-mediated feedback regulation of Cyp7a1.</P>

Prognostic factors for outcomes of allogeneic stem cell transplantation in chronic phase chronic myeloid leukemia in the era of tyrosine kinase inhibitors

Lee, Sung-Eun,Choi, Soo Young,Kim, Soo-Hyun,Jang, Eun-Jung,Bang, Ju-Hee,Byeun, Ji-Young,Park, Jin Eok,Jeon, Hye-Rim,Oh, Yun Jeong,Yahng, Seung-Ah,Cho, Byung-Sik,Eom, Ki-Sung,Kim, Yoo-Jin,Lee, Seok,Min Informa UK (TaylorFrancis) 2014 Hematology Vol.19 No.2

The aim of this study was to estimate the prognostic factors for the outcomes of chronic myeloid leukemia (CML) patients receiving allogeneic stem cell transplantation (SCT) in chronic phase (CP) in the era of tyrosine kinase inhibitors (TKIs). Ninety-seven patients who underwent allogeneic SCT in CP were analyzed. Forty-seven were TKI-naive at the time of transplant, and 50 received TKI(s) treatment before transplantation. After a median follow-up of 115.8 months, the 4-year overall survival and event-free survival were 80.4 and 58.8%, respectively. Multivariate analysis showed that there were no differences in survival outcomes based on prior TKI therapy. Older age was a prognostic factor for higher treatmentrelated mortality (TRM), and the type of graft source and younger age were associated with relapse, but prior TKI therapy and disease status at the time of transplant were not associated with either TRM or relapse. Additionally, a major molecular response at 1 month and an MR4.5 at 3 months were important predictors of favorable long-term outcomes. This study demonstrates the prognostic factors for the outcomes of allogeneic SCT in CP CML and shows that survival outcomes were not affected by the administration of long-term multi-TKI treatment prior to transplantation.

전정기의 온도자극에 의한 위전도 변화

이문영,김민선,박병림,나용호,최석채,김진아,서검석 대한소화기학회 1998 대한소화기학회지 Vol.32 No.3

Background/Aims: It has been known that motion sickness cause gastrointestinal symptoms including nausea, vomiting, oculomotor and postural asymmetry. The purpose of this study was to characterize the changes of electrogastrographic patterns elicited by thermal stimulation of the vestibular system. Methods: Thirty three subjects (age:21-30, M: 20, F:13) were divided into three groups: the group I (n=11) was treated with single ice water stimulation (3℃) on unilateral vestibular system (ICE), group II (n=l 1) with single cold water (C, 30℃) and warm water (W, 43℃) stimulation (CW), and group III (n=11) with 2 times cold and warm water stimulation on bilateral vestibular systems (CCWW). Gastric electrical activity was recorded by using EGG with Ag-AgCl cutaneous electrodes. Results: Gastric symptoms such as gastric discomfort, nausca, and vomiting after these thermal stimulations were not observed in the group I. However, the symptoms developed in 4 subjects of the group II (36%) and 11 subjects of the group III (82%). Thermal stimulation in the group II and III increased percentage of tachygastria (goup II, 16.4±3.7 to 36.9±6.7; group III, 15.6±4.8 to 35.0±6.8) and decreased significantly percentage of 3 CPM (group II, 78.7±to 53.9±6.8; group III, 80.6±5.0 to 49.0±8.0)(p$lt;0.05). Canclusions: These results suggest that thermal stimulation of the peripheral vestibular system induce motion sickness symptoms, and tachygastria is more prominent in the presence of gastric symptoms.

A Potential Cytotoxic Principle of Zingiber cassumunar

Han, Ah-Reum,Lee, Eun-Jin,Min, Hye-Young,Kim, Hyang-Rim,Lee, Sang Kook,Seo, Eun-Kyoung 梨花女子大學校 藥學硏究所 2003 藥學硏究論文集 Vol.- No.12

A bioassay-guided fractionation of the active chloroform extracts of the rhizomes of Zingiber cassumunar Roxb. led to the isolation of a potential cytotoxic principle, curcumin (1), along with two inactive compounds, (E)-4-(3',4'-dimethoxyphenyl)but-3-en-l-ol (2) and (E)-4-(3',4'-dimethoxyphenyl)but-3-en-l-yl acetate (3). Curcumin (1) showed a significant cytotoxicity against several human cancer cell lines (Col2; 2.30, A549; 12.30, SNU638; IC_(50) 18.80㎍/㎖).

A novel antibacterial peptide in Apis mellifera against the enteric pathogenic Escherichia coli

Hyun Rim Oh,Hye-Kyung Kim,Mi Ra Lee,Man-young Lee,Ah Rang Kang,Soo Eun Kang,Tae Ho Oh,Seong Hee Kim,Yong-Soo Choi 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.10

Insect peptides have been extensively studied due to beneficial effects in the treatment of infectious diseases. Melittin, a fundamental component of honeybee venom produced by European honeybee Apis mellifera, has applied to prevent various inflammatory disease and bacterial infections in human. However, the therapeutic application of melittin is limited due to its low stability, hemolytic activity and expensive manufacturing costs. In this study, we aimed to discovery unknown peptides from the Apis mellifera and evaluate its antibacterial activity against Escherichia coli KACC 10005. A total 15,853 peptide sequences were diciphered using Illumina HiSeq 2500 next-generation sequencing (NGS) platform and analyzed based on the Apis mellifera official Gene Set Version 3.2 (amel_OGSv3.2) and the Collection of Anti-Microbial Peptides (CAMPR3) database. All the peptide sequences and annotation data sets were combined and sorted by physicochemical features of antimicrobial peptides (AMPs), such as short peptide length <=50, positive charge, isoelectric point (8.0<=pl<=12), and aggregation propensity (in-vitro: <=500, in-vivo: –40<= Na4vSS <=60). Among the screened peptides, four unknown peptide candidates, named AMP1-4, were chemically synthesized and tested for antimicrobial activity in comparison with a reference peptide, melittin. Inhibition of bacterial growth was observed in the AMP4 treated group from 6 hours to 48 hours post-treatment against E. coli. These results suggest that honeybee-derived peptide sequences can be applied as natural resources to acquire novel AMPs and the peptide sequences derived parameters are enough to recognize antibacterial peptides. In addition, the selected novel peptide candidate, AMP4, has antibacterial activity.