해면 Spirastrella abata와 Cinachyrella sp.의 공생 세균의 계통학적 다양성

조현희,심은정,박진숙,Cho, Hyun-Hee,Shim, Eun-Jung,Park, Jin-Sook 한국미생물학회 2010 미생물학회지 Vol.46 No.2

2009년 4월 제주도의 운진항에서 채집한 해양 해면 Spirastrella abata와 Cinachyrella sp.의 공생 세균의 주요 군집구조를 16SrDNA-denaturing gradient gel electrophoresis (DGGE) 방법을 이용하여 비교 분석하였다. S. abata와 Cinachyrella sp.는 각각 7개와 8개의 DGGE 밴드를 나타내었으며 이들을 적출하여 염기서열을 분석한 결과, NCBI에 등록된 서열들과 92-100%의 유사도를 나타내었다. S. abata의 주요 공생세균은 Alphaproteobacteria와 Deltaproteobacteria에 속하였으며, Cinachyrella sp.의 경우 Alphaproteobacteria, Gammaproteobacteria와 Actinobacteria에 속하는 세균으로 구성되었다. 두 종의 해면에서 공통되는 공생세균 그룹은 Alphaproteobacteria였으며 이 세균 그룹은 두 종의 해면 모두에서 우점하였다. Deltaproteobacteria는 S. abata에서, Actinobacteria와 Gammaproteobacteria는 Cinachyrella sp.에서만 관찰되었다. 동일지역에서 채집한 서로 다른 두 종의 해면은 각각 다른 공생세균 군집구조를 나타내었다. The bacterial community structure of two marine sponges, Spirastrella abata and Cinachyrella sp. collected from Jeju Island, in April 2009, was analyzed by 16S rDNA-denaturing gradient gel electrophoresis (DGGE). DGGE banding patterns indicated 8 and 7 bands for Spirastrella abata and Cinachyrella sp., respectively. Comparative sequence analysis of variable DGGE bands revealed from 92% to 100% similarity to the known published sequences. The bacterial groups associated with Spirastrella abata were Alphaproteobacteria and Deltaproteobacteria. The bacterial community of Cinachyrella sp. consisted of Alphaproteobacteria, Gammaproteobacteria, and Actinobacteria. Alphaproteobacteria was common and predominant in both the sponge species. Deltaproteobacteria was found only in Spirastrella abata while Actinobacteria and Gammaproteobacteria were found only in Cinachyrella sp. The results revealed that though the common bacterial group was found in both the sponges, the bacterial community profiles differed between the two sponge species obtained from the same geographical location.

16S rDNA-RFLP에 의한 Spirastrella abata와 Spirastrella panis 해면에 서식하는 배양가능한 공생세균 군집의 비교

조현희,박진숙,Cho, Hyun-Hee,Park, Jin-Sook 한국미생물학회 2009 미생물학회지 Vol.45 No.2

계통적으로 근연하며 지리적 분포가 유사한 두 종의 Spirastrella 속의 해양 해면, S. panis와 S. abata의 배양 가능한 공생세균 군집구조를 16S rDNA-RFLP 방법에 의해 분석하였다. 공생세균의 배양은 해면추출물 3%를 포함하는 MA 배지를 사용하였다. 증폭된 16S rDNA의 RFLP (restriction fragment length polymorphism) 분석을 위한 제한효소로 HaeIII와 MspI을 이용하였으며, 그 결과 24개의 RFLP type을 구별할 수 있었다. 각 패턴별로 1~5개의 분리균주를 선별하여 부분 염기서열 분석 결과, 알려진 세균 종과 98.4% 이상의 유사도를 나타내었으며 2종의 Spirastrella 해면으로부터 분리된 세균들은 모두 Alphaproteobacteria, Gammaproteobacteria, Firmicutes, Actinobacteria 4개의 강(class)에 포함되었다. Alphaproteobacteria는 S. abata에서 39.3%, S. panis에서 47.6%가 관찰되어 두 해면에서 우점하는 세균 군집이었다. Gammaproteobacteria의 경우 S. abata에서 38.5%로 관찰된 반면 S. panis에서 1.6%의 아주 적은 비율로 관찰되었다. 또한 Bacillus (phylum Firmicutes) 종은 S. abata에서 9.7%를 나타낸 반면, S. panis에서는 44.3%의 분포를 나타내었다. Planococcus maritimus (8.1%, phylum Firmicutes)와 Psychrobacter nivimaris (28.9%, phylum Gammaproteobacteria)는 S. abata에서만 관찰되어 이들은 S. abata에 특이적인 세균 종임을 알 수 있었다. 같은 장소에 서식하는 계통적으로 근연한 두 종의 해면에서 공생세균의 군집 구조는 차이가 큰 것으로 나타났다. A cultivation-based approach was employed to compare the culturable bacterial diversity associated with two phylogenetically closely related marine sponges, Spirastrella abata and Spirastrella panis, which have geologically overlapping distribution patterns. The bacteria associated with sponge were cultivated using MA medium supplemented with 3% sponge extracts. Community structures of the culturable bacteria of the two sponge species were analyzed with PCR-RFLP (restriction fragment length polymorphism) based on 16S rDNA sequences. The RFLP fingerprinting of 16S rDNA digested with HaeIII and MspI, revealed 24 independent RFLP types, in which 1-5 representative strains from each type were partially sequenced. The sequence analysis showed >98.4% similarity to known bacterial species in public databases. Overall, the microbial populations of two sponges investigated were found to be the members of the classes; Alphaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria. The Alphaproteobacteria were predominant in the bacterial communities of the two sponges. Gammaproteobacteria represented 38.5% of bacterial community in S. abata. Whereas only 1.6% of this class was present in S. panis. Bacillus species were dominat in S. panis. Bacillus species were found to be 44.3% of bacterial species in S. panis, while they were only 9.7% in S. abata. It is interesting to note that Planococcus maritimus (8.1%, phylum Firmicutes) and Psychrobacter nivimaris (28.9%, phylum Gammaproteobacteria) were found only in S. abata. This result revealed that profiles of bacterial communities from the sponges with a close phylogenetic relationship were highly species-specific.

보비탕(補脾湯)이 methotrexate로 유발(誘發)된 흰쥐의 면역기능저하(免疫機能低下)에 미치는 영향(影響)

조현희,윤혜진,서정민,백정한,Cho, Hyun-Hee,Yun, Hye-Jin,Seo, Jung-Min,Baek, Jung-Han 대한한방소아과학회 2007 대한한방소아과학회지 Vol.21 No.1

Objectives : In order to investigate the effect of Bobitang on SD rats with deteriorated immunity caused by methotrexate. Methods : The test sample were dosed once a day for 14 days by gastric gavage at a dosage 1,000, 500 and $250mg/kg/10m{\ell}$ from 2 days after last MTX-dosing, and the changes on body weight and gains, spleen weight and total blood leukocyte numbers, total lymphocyte numbers, the percentage of B-cell, T-cell, CD3+CD4+ T-cell, CD3+CD8+ T-cell and CD4+/CD8+ T-cell ratios in the blood and spleen were observed. Results : The changes on body weight gains, the spleen weight, the total blood leukocyte numbers, the total lymphocyte numbers in the blood and spleen, the ratio of T-cell in the blood and spleen, the ratio of CD3+CD4+ T-cell in the blood and spleen were increased significantly in BBT Extracts groups as compared with control group. The ratio of B-cell in the blood and spleen was not increased significantly in BBT Extracts groups as compared with control group. The percentage of CD3+CD8+ T-cell in the blood and spleen was decreased significantly in BBT Extracts groups as compared with control group. The ratio of CD4+/CD8+ T-cell in blood and spleen was increased significantly in BBT Extracts group as compared with control group. Conclusions : According to the above results, Bobi-Tang has an effect of increasing immune responses on SD rats with deteriorated immunity caused by methotrexate.

꼬리뼈하 천골고정술: 자궁탈출증 치료의 단기성적보고

조현희 ( Hyun Hee Cho ),김미란 ( Mee Ran Kim ),황성진 ( Sung Jin Hwang ),김민정 ( Min Jung Kim ),김은중 ( En Jung Kim ),이진우 ( Jin Woo Lee ),김장흡 ( Jang Heub Kim ),김진홍 ( Jin Hong Kim ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.6

목적: 자궁탈출증 및 질말단부탈출증으로 꼬리뼈하 천골고정술을 시행받은 34명의 여성의 6개월 단기추적결과를 보고하고자 한다. 연구방법: 자궁탈출증 및 질말단부탈출증이 있는 여성 34명에서 꼬리뼈하 천골고정술을 시행하여 수술 중/ 수술 후 나타날 수 있는 합병증과 수술 6개월 뒤 재발 여부에 대해 추적검사를 실시하였다. 결과: 꼬리뼈하 천골고정술 후 혈색소의 변화는 1.7±0.9로 나타났으며, 도뇨관 유치기간은 3.1±1.0로 수술 후 평균 2.1일에 도뇨관을 제거하고 자가배뇨가 가능하였다. 입원기간은 5.2±1.0로 수술 후 평균 3.2일이면 퇴원이 가능하였다. 6개월 재발율은 2.9%였다. 수술 전 환자들의 자궁/질말단부 탈출증의 정도는 평균 2.7±0.7 (stage)였으며, 수술 후 6개월에 확인한 탈출증의 정도는 0.4±0.6 (stage)이었다. 결론: 꼬리뼈하 천골고정술은 자궁적출술 없이 효과적으로 자궁탈출증을 치료할 수 있는 방법이다. Objective: To investigate the short term results of infracoccygeal sacropexy for the management of uterine prolpase or stump prolapse. Methods: Thirty-four Women who underwent infracoccygeal sacropexy for the management of uterine prolapse or stump prolpase were enrolled this study. Intraoperative or postoperative complications and results of the operation were evaluated and the recurrence rate of pelvic organ prolapse was checked 6 months after operation. Results: Changes of hemoglobin after infracoccygeal sacropexy was 1.7±0.9, mean duration of bladder drainage was 3.1±1.0 (days). After 2.1 days of operation, foley catheter was removed. Mean inpatient day was 5.2±1.0, patient discharged 3.2 days after operation. Recurrence rate after 6 months was 2.9%. Preoperative mean stage of prolapse was 2.7±0.7 (stage) and it decreased to 0.4±0.6 (stage) 6 months after operation. Conclusions: Infracoccygeal sacropexy was effective method for the management of uterine/ stump prolapse.

탄소나노튜브(CNT)-PVDF 막을 이용한 자연용존유기물 제거

조현희 ( Hyun Hee Cho ),차민환 ( Min Whan Cha ),박재우 ( Jae Woo Park ) 한국물환경학회 2012 한국물환경학회지 Vol.28 No.1

In this research, the application of carbon nanotubes (CNTs) modified PVDF (polyvinylidene fluoride) membrane was tested as a simply and beginning attempt to overcome membrane fouling because CNTs importantly affect the transport of natural organic matter (NOM). Suwannee River fulvic acid (SRFA) as the representative of NOM was selected and its sorption results with single-walled CNT (SWCNT), multi-walled CNT (MWCNT), and oxidized MWCNT (O-MWCNT) were obtained through the batch experiment. SRFA sorption isotherms had a strong nonlinearity and its sorption capacity followed the order O-MWCNT < MWCNT < SWCNT. The adsorbed mass of SRFA on each CNT decreased as a function of pH due to their charge repulsion. For the CNT-PVDF membrane filtration experiments, the suspended CNT solution (10 mg/40 mL) was incorporated into 0.45 μm-PVDF membrane and 5 mg/L of SRFA solution was monitored using UV detector connected with high pressure pump after passing through CNT-PVDF membrane. The SRFA removal efficiency by MWCNT-PVDF membrane was the strongest among other modified membranes. This suggests that the CNT modified microfiltration (MF) membrane might effectively and selectively apply to treat the contaminated water including organic compounds in the presence of NOM.

한국여성에서 조직 미네랄 농도에 따른 생리전증후군의 임상증상

조현희 ( Hyun Hee Cho ),정인철 ( In Chul Jung ),정재은 ( Jae En Jung ),최세경 ( Sae Kyung Choi ),김수연 ( Su Yeun Kim ),김미란 ( Mee Ran Kim ),임용택 ( Yong Taek Lim ),김은중 ( En Jung Kim ),김진홍 ( Jin Hong Kim ) 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.1

목적: 생리전증후군이란, 황체 후반기에 특정한 정신적, 육체적 증상이 주기적으로 나타나는 질환을 말하며, 발생율은 80%에 이르나 원인은 명확하게 밝혀져 있지 않다. 프로게스테론과 에스트로겐이 체내 미네랄의 균형에 영향을 주기 때문에 생리전증후군 환자들에서 나타나는 프로게스테론의 감소와 에스트로겐이 우세 현상은 조직 내 미네랄의 균형에 이상을 초래할 수 있으며, 이로 인해 생리전증후군의 여러 특이 증상이 유발될 가능성이 있다. 이에 저자는 환자의 조직 미네랄 검사를 통해, 생리전증후군이 있는 환자에서 미네랄 수치와 증상과의 연관성을 알아보고자 본 연구를 시도하였다. 방법: 생리전증후군에 대한 모발검사 연구 지원자는 공고를 통해 모집된 여성을 대상으로 MMDQ 설문지를 작성하도록 하여 총점 10점 이상인 여성 120명으로 대상으로 하였다. 모발을 이용한 조직미네랄 검사는 최근 3주간 염색이나 파마를 하지 않고 3시간 이내에 세정이나 모발관련 제품을 사용하지 않은 상태에서 시행되었으며, 미국 TEI에 분석을 의뢰하였다. 통계분석은 SPSS (Ver. 14.0)를 사용하였다. 결과: 조직 내 마그네슘 농도가 정상인 군의 MMDQ 총점은 비정상인 군보다 유의하게 높았으며 (22.5±17.8 vs. 13.9±11.1), 나트륨 농도가 정상인군의 행동장애 점수는 비정상군보다 유의하게 낮게 나타났다 (2.2±1.7 vs. 3.4±2.2). 또한 구리 농도가 정상인군의 MMDQ 총점은 비정상인 군보다 유의하게 낮았다 (15.7±11.9 vs. 24.9±16.9). 고찰: 조직 내 마그네슘과 구리의 농도에 따라 MMDQ 총점에서 차이를 보였으며, 조직 내 나트륨 농도는 행동장애 점수에 영향을 주는 것으로 나타났다. 그러나 이러한 미네랄 불균형이 생리전증후군의 증상을 악화시키는 원인으로 작용하는지에 대해서는 좀 더 자세한 연구가 필요할 것으로 사료된다. Objective: Premenstrual syndrome (PMS) is a disease with specific psychologic and physical symptoms on luteal phase. Its incidence is variable in 20~80%, but its cause is not definitely proved. Because progesterone and estrogen affect the balance of the body mineral, the alteration of progestorone and estrogen in the patients with PMS may effect the imbalance of tissue mineral, that can induce the specific symptoms of PMS. This study examines the relationship between symptoms of PMS and mineral count by tissue mineral test. Methods: Women who volunteered for the tissue mineral test completed MMDQ questionnaire and checked blood test for Ca, Mg, Na, K, Cu, Zn. The tissue mineral test used the hair not treated within 3 weeks and not washed within 3 hours. The hair was send to TEI for the analysis. We used SPSS (14.0) for statistical analysis. Results: The MMDQ score of the normal Mg group is significantly higher than the high Mg group (22.5±17.8 vs. 13.9±11.1), and the behavioral disorder score of the normal Na group is significantly lower than the low Na group (2.2±1.7 vs. 3.4±2.2). The MMDQ score of the normal Cu group is significantly lower than abnormal group (15.7±11.9 vs. 24.9±16.9). Conclusions: Total score of MMDQ showed difference according to magnesium and copper concentrations in tissue, and scores of behavioral disorder was affected by sodium concentration of tissue. Additional study about cause and effect relationship is required.

치유된 대상포진 부위에 발생한 혈관육종 Wolf`s Isotopic Response

조현희 ( Hyun Hee Cho ),백승환 ( Seung Hwan Paik ),변희진 ( Hee Jin Byun ),조광현 ( Kwang Hyun Cho ) 대한피부과학회 2012 대한피부과학회지 Vol.50 No.4

Angiosarcoma is a rare malignant vascular tumor of endothelial origin. It can involve any organ, including the skin, soft tissue, liver, bone, spleen, and orbit. Skin is the most frequent site of primary angiosarcoma, however, metastatic skin involvement of angiosarcoma is exceptional. Wolf`s isotopic response implies that the new disease appeared at the site of an already healed skin disease. Several types of cutaneous lesions have been described, occurring within cleared cutaneous herpes zoster, or herpes simplex lesions. These lesions include comedones, granulomatous diseases, tinea, furunculosis, wart, psoriasis, pseudolymphoma, leukemia, lymphoma, basal cell carcinoma, squamous cell carcinoma and cutaneous metastases, from internal carcinoma. Herein, we report a case of cutaneous angiosarcoma of unknown origin that has developed at the site of a scar, after herpes zoster in a 79-year-old man as isotopic response. (Korean J Dermatol 2012;50(4):367∼370)

초등영어 교사의 교수,학습에 대한 신념의 양상과 역할

조현희 ( Hyun Hee Cho ),김형준 ( Hyung Jun Kim ) 한국현대영어영문학회 2010 현대영어영문학 Vol.54 No.1

This study explored four elementary school teachers` beliefs about teaching English and discovered how their beliefs influenced their teaching practice. An in-depth individual interview was conducted for the study. The study demonstrates that teachers had some beliefs in common, while other beliefs differed between one another. Their common beliefs were shaped by the collective experience of teaching English in the elementary school. The beliefs that differed between individual teachers were formed by their personal teaching and learning experiences as well as local contexts of the classroom. It was discovered that the teachers made efforts to put their beliefs into practice using whatever resources that were available to them. They could not implement what they believed, however, when it involved global social context. This study suggests that teachers` voice should be heard in order to improve elementary English education, and that more attention should be paid to teachers` beliefs in teacher education programs. (Daegu National University of Education, Daegu Cheong-rim Elementary School)

과학문화에 대한 확장적 개념화와 해외 바우처 사례분석을 통한 과학문화 바우처 활성화 방안 탐색: 다학제적 접근

조현희(Cho, Hyun hee),우라미(Woo, Ra mi),장신호(Jang, Shin ho),김근희(Kim, Keun hee),나훈희(Na, Hun hee) 한국전시산업융합연구원 2021 한국과학예술융합학회 Vol.39 No.2

본 연구는 과학문화를 활성화하기 위한 기제로서 바우처가 지닌 가능성에 착안하여 시작되었다. 본 연구의 목적은 과학문화 바우처에 대한 이론적 논의와 해외 바우처 사업 운영사례에 대한 분석을 바탕으로 바우처를 활용한 과학문화 활성화 방안을 모색하는데 있다. 이를 위해 본 연구에서는 과학문화의 개념 및 바우처 사업 운영에 관한 문헌자료와 선행연구를 분석함으로써 과학문화를 이해하기 위한 개념적 틀을 도출하고, 해외 주요국의 성공적인 바우처 운영 사례를 살펴보았다. 연구 결과는 다음과 같다. 첫째, 바우처를 통한 과학문화의 양적 확대와 질적 성장을 도모하기 위해 기존 바우처 사업에 적용되어 온 유사시장론(quasi-market)을 넘어 서비스산업화와 신(新)산업 육성에 중점을 둔 사업 운영이 필요한 것을 알 수 있었다. 둘째, 해외 주요국(미국, 프랑스, 네덜란드)의 사례를 분석한 결과, 과학문화 바우처 사업 운영에 있어 통합성을 강화한 인프라 구축, 과학문화의 양적, 질적성장을 동시에 고려한 관계(network) 및 기술(technical) 차원의 지원, 자원봉사를 통한 범시민적 참여확대 등이 중요함을 알 수 있었다. 이러한 연구 결과를 바탕으로 과학문화의 양적, 질적 성장과 과학문화 산업의 육성을 도모하기 위한 과학문화 바우처 사업의 방향과 과제들을 논의하였다. This research is based on the potential of vouchers as a mechanism to stimulate the science culture of South Korea. The purpose of this study is to explore strategic plans to stimulate the science culture utilizing vouchers, based on theoretical discussion and overseas cases of culture vouchers. The concepts of science culture and administrative plans for vouchers are analyzed through the previous studies and literature. This research shows that existing voucher plans need to move beyond quasi-market framework toward advanced voucher plans focused on industrializing services and fostering new industries in order to achieve expansion and internal reinforcement of science culture. Based on the overseas cases of the United States, France, and the Netherlands, this research presents the importance of building integrated-infra networks, and technical and relational support policy for achieving expansion. The authors also address the necessity to focus on internal reinforcement of science culture, and encouraging citizens to participate in the science voucher programs. Based on the results, we expect to invigorate the science culture voucher policy and eventually expand prospective space for nurturing science culture industries throughout the nation.

거제시 수돗물불소농도조정사업 6년 경과 후의 영구치우식 예방효과

조현희 ( Hyun-hee Cho ),옥태영 ( Tae-young Ok ),김세연 ( Se-yeon Kim ),이정하 ( Jung-ha Lee ),김지수 ( Ji-soo Kim ),김현철 ( Hyeon-cheol Kim ),정승화 ( Seung-hwa Jeong ),김진범 ( Jin-bom Kim ) 대한예방치과·구강보건학회 2017 大韓口腔保健學會誌 Vol.41 No.4

Objectives: The water fluoridation program has been implemented since 2008, in a region of Geoje City. This study aimed to evaluate the effectiveness of the water fluoridation program on caries prevention in the permanent dentition. Methods: Evaluation surveys were conducted on 8-, 10- and 12-year-old children in the fluoridated and non-fluoridated regions of Geoje City, in 2015. The survey included 834 children from the fluoridated regions and 703 from the non-fluoridated regions. The data obtained from the survey were analyzed using the IBM SPSS statistical package version 23.0. The effectiveness of community water fluoridation in caries prevention was estimated by the differences in the decayed, missing, filled teeth (DMFT) and decayed, missing, filled surfaces (DMFS) scores, between the program and control groups. The mean numbers of fissure-sealed teeth and surfaces were adjusted to reduce their confounding effect. Results: The mean DMFT and DMFS scores (0.69 and 0.91, respectively) of 10-year-olds from the fluoridated regions, after adjusting for the numbers of fissure-sealed teeth and surfaces, were significantly lower than those of the 10 year olds (DMFT: 0.95, DMFS: 1.32) from the non-fluoridated regions. The effectiveness of water fluoridation on caries prevention in the permanent dentition, estimated based on the differences in the mean DMFT and DMFS scores, adjusted for the number of fissure-sealed teeth and surfaces, between the program and control groups of 10-year-old children, was 27.4% and 31.1%, respectively. Conclusions: These results suggest that the effectiveness of water fluoridation on caries prevention in the permanent dentition, in Geoje City was so high that this program should be implemented in other regions in Korea as well.