‘후지’, ‘시나노 스위트’ 및 ‘갈라’ 품종에서의 약제적화

조광현(Kwang-Hyun Cho),한수곤(Su-Gon Han),윤태명(Tae-Myung Yoon) 한국원예학회HST 2011 원예과학기술지 Vol.29 No.별호1

건선 피부병소에서 표피세포의 증식 및 분화 - 건선 표피에서 DNA 합성세포 및 분화 표식자 발현의 검색 -

조광현(Kwang Hyun Cho),장승호(Seung Ho Chang),김영걸(Young Gull Kim),윤재일(Jai Il Youn),박상철(Sang Chul Park),함의근(Eui Keun Ham) 대한피부과학회 1992 대한피부과학회지 Vol.30 No.3

DNA replicating cells were detected in the psoriatic epidermis by anti BrdU antibody after incubating tissue sections biopsied in acute and chronic areas with BrdU. The expression pattern of No. 1 keratin, involucrin. filaggrin, loricrin and transglutaminase E was studied in the paraffin embedded sections of psoriatic specimen which showed various histopathological findings. The results are as follows 1) The labeling index of BrdU in lesional psoriatic skin was significantly greater than that in normal skin. The labeling index was greater in the clinically active lesion of psoriasis than that of the chronic lesion, which suggest that psoriatic lesions are composed of distinct lesions differing in activity. 2) In the epidermis of the psoriatic plaques with extensive parakeratosis or microabscesses at the base of the stratum corneum and absent granular layers, the expression pattern of five epidermal proteins appeared as follows : No. 1 keratin which is found in normal epidermis immediately above the basal layer, appeared several layers higher. Involucrin was detected in most of the suprabasal layers. Fillaggrin, loricrin and transglutaminase E showed negative expression. The results obtained in the present study suggest that the increment of DNA replicating cells and aberrant, maturation pathway of epidermis appear in active psoriatic plaques. (Kor J Dermatol 1992; 30(3): 291-302)

피지선 모반의 표피에서 케라틴 및 Involucrin 발현

조광현(Kwang Hyun Cho),김영걸(Young Gull Kim),조미경(Mi Kyung Cho),지제근(Je Geun Chi) 대한피부과학회 1992 대한피부과학회지 Vol.30 No.3

Thirty two cases of nevus sebaceus were studied by immunohistochemical staining employing three anticytokeratin antibodies(34bE, 34bB, CAM 5.2) and anti-involucrin antibody in order to clarify the biochemical characteristics of the covering epidermis of nevus sebaceus. An attempt was made to compare the expression pattern of these proteins in the epidermis of nevus sebaceus with that in normal skin and in epidermal nevus. Serial sections in all cases were also stained with PAS in attempt to correlate these protein expression with the amount of glycogen in the epidermis of nevus sebaceus. The following results are obtained. 1. The expression of cytokeratin and involucrin in the epidermis of nevus sebaceus was changed as the age of the lesions increased, but we could not identify a uniform pattern according to the age of lesions. 2. Several CAM 5.2 positive cells were found in the basal layer of the epidermis of nevus sebaceus lesion obtained from patients over 10 years of age. 3. The expression of cytokeratin and involucrin did not show any correlation with glycogen content. These results suggest that the covering epidermis of the lesion of nevus sebaceus may not be nevoid proliferation of epidermal nevus. (Kor J Dermatol 1992; 30(3): 279-290)

Bazin의 경결홍반

조광현 ( Kwang Hyun Cho ) 대한피부과학회 2010 대한피부과학회지 Vol.48 No.12

Erythema induratum of Bazin (EIB) belongs to a group of diseases characterized by inflammatory nodules of the leg which primarily involve the lower legs. EIB is considered to be a reactive disorder related to several etiologic factors. In Korea, most dermatologists accept the tuberculosis as one of the most common etiology of EIB. As the number of EIB cases is decreasing in Korea in accordance with the decreased incidence of tuberculosis, EIB can be easily mistaken for any of the disease that produces chronic nodular eruptions on the legs. In Korea, important factors to make a correct diagnosis of EI include clinicopathologic observations coupled with the identification of hypersensitivity to tuberculous antigen and successful treatment of anti-tuberculous medication. (Korean J Dermatol 2010;48(12):1045∼1051)

안면 및 두피에 발생한 혈관육종

조광현(Kwang Hyun Cho),강승주(Seung Joo Kang),이유신(Yoo Shin Lee),김철우(Chul Woo Kim) 대한피부과학회 1986 대한피부과학회지 Vol.24 No.6

We report a case of angiosarcoma of the face and scalp in a 76-year-old man. Immunochemical staining with factor 8-related antigen and ultrastructural find ings confirm that this tumor was origined from endothelium. The patient had a good initial response to treatment with radiotherapy.

황색 조갑 증후군

조광현(Kwang Hyun Cho),이주홍(Joo Heung Ree),이유신(Yoo Shin Lee),한성구(Sung Koo Han) 대한피부과학회 1988 대한피부과학회지 Vol.26 No.4

We report a 58 year old man who complained that his nails didn't grow for a year and developed yellowish discoloration. On physical examination his nails showed yellow greenish discoloration, increased corvexity, loss of lunulae and cuticles. C]iest X ray revealed atelectasis on right lower lung field and bronchiectasis on left lower lung field. Mild restrictive pattern was observed in pulmoniry function test. We gave him 800IU of Vitamin E claily for 3 months, but rio remarkable changes have been observed as yet..

상피내 말단흑자흑색종

조광현 ( Kwang Hyun Cho ),박현선 ( Hyun Sun Park ) 대한피부과학회 2010 대한피부과학회지 Vol.48 No.7

Acral lentiginous melanoma (ALM) is the most common subtype of melanoma in Asian countries including Korea. ALM was considered more aggressive than other subtypes, but recent reports suggest that it was because of late detection or delayed diagnosis, rather than a true biological difference. Therefore, early diagnosis of ALM is important for a better prognosis but its diagnosis is not always easy. There are scarce reports upon ALM in situ, probably due to its rarity and diagnostic difficulty, which makes in depth understanding and proper management of ALM in situ complicated. Therefore, we have reviewed our experience for twenty years with acral pigmented lesions showing intraepidermal proliferation of melanocytes which was diagnosed or suspected as ALM in situ. (Korean J Dermatol 2010;48(7):551∼557)

새로운 항암제에 의한 피부부작용

조광현 ( Kwang Hyun Cho ) 대한피부과학회 2010 대한피부과학회지 Vol.48 No.4

Chemotherapeutic agents may induce cutaneous toxicity. Although the cutaneous side effects of chemotherapeutic agents are rarely life threatening, they can impact the physical, psychological, and social well-being of patients receiving these therapies. Recently, a number of new anti-cancer agents have been approved for the treatment of cancer. These new anti-cancer agents manifest a variety of cutaneous reactions. This article reviews the cutaneous toxicity of these agents, particularly those which dermatologists are likely to be consulted about. (Korean J Dermatol 2010;48(4):257~265)

피부과 자문

조광현 ( Kwang Hyun Cho ) 대한피부과학회 2010 대한피부과학회지 Vol.48 No.3

토마토 뿌리조작에서 분리한 마이크로솜의 Ca2+ 흡수 특성

조광현(Kwang Hyun Cho),김영기(Young Kee Kim) 한국응용생명화학회 1999 Applied Biological Chemistry (Appl Biol Chem) Vol.42 No.2

In order to characterize the property of Ca^(2+) transport in plant cell, microsomes were prepared from the roots of tomato and microsomal ^(45)Ca^(2+) uptake was measured. When 1 mM vanadate, a selective inhibitor of P-type ATPases, 50 mM NO₃^-, a specific inhibitor of vacuolar H^+-ATPase, and both of these inhibitors were treated, the microsomal ^(45)Ca^(2+) uptakes were inhibited by 20, 33 and 47%, respectively. The inhibitory effects of these two inhibitors were investigated by using a protonophore, gramicidin. When the chemical gradient of H^+ was relieved by gramicidin, the uptake was decreased by 30%, implying the presence of Ca^(2+)/H^+ antiporter in the microsomal membrane. In the ^(45)Ca^(2+) uptake experiment, the effect of gramicidin was independent of vanadate-induced inhibition. However, when the activity of vacuolar H^+-ATPase was inhibited by NO₃^-, the effect of gramicidin was severely decreased. Meanwhile, thapsigargin, a specific antagonist of ER/SR-type Ca^(2+)-ATPase, inhibited the microsomal ^(45)Ca^(2+) uptake and the maximum inhibitory effect was obtained at 10 μM. The effect of thapsigargin was blocked by NO₃^- and gramicidin, but not by vanadate. These results imply that vanadate directly inhibits the activity of Ca^(2+)-ATPase; however, NO₃^- and thapsigargin block the activity of Ca^(2+)/H^+ antiporter by inhibiting the vacuolar H^+-ATPase. In conclusion, the microsomal ^(45)Ca^(2+) uptakes are mediated by two major enzymes, Ca^(2+)-ATPase and Ca^(2+)/H^+ antiporter in tomato root tissue.