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새로 발생한 13 q32.2 → qter 결손과 동반된 13 번 환상염색체 환아 1 예
황성진(Seong Jin Hwang),이지현(Jee Hyun Lee),박인양(In Yang Park),문희봉(Hee Bong Moon),오준환(Joon Hwan Oh),이귀세라(Gui Se Ra Lee),이종승(Chong Seung Yi),신종철(Jong Chul Shin),김수평(Soo Pyung Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.2
We report a case of ring chromosome 13 with a distal deletion of 13q32.2→qter observed in a fetus who was referred to our institution at term due to severe growth restriction and multiple congenital malformations on ultrasonographic examination. This boy was born by vaginal delivery at 39 weeks in gestation. His weight, head circumference and height were less than the 3 percentile of gestational age. Apgar score was 7 at 1 minute and 9 at 5 minutes. He showed microcephaly, large forehead, low set ears, hypertelorism, flat nasal bridge, and micrognathia. The genitalia was ambiguous, showing severe hypoplasia of the penis. The anus was ectopic, displaced anteriorly from its normal position but with a normal opening and function. Neurologic examination was normal. Echocardiogram done at 2 weeks of life showed a persistent foramen ovale and a ventriculoseptal defect (type II) with increased pulmonary hypertension. MRI examination of the brain showed poorly demarcating corpus callosum suspecting agenesis of corpus callosum. Also, cerebellar vermis was small and hypoplastic, mimicking a variant form of Dandy-Walker malformation. MRI of the pelvis showed a tubular structure in pelvic cavity, suspicious of uterine remnant, between urinary bladder and rectum, and a inguinal hernia was noted in the left side. In the abdominal cavity enlarged adrenal glands were noted, and hormonal study showed elevated 17-α-OH-progesterone (168.9 ng/ml) with normal 17-KS and 17-OHCS levels. Gastrointestinal and urogenital system were otherwise normal. Cytogenetic analysis of the parents were both normal but the newborn showed 46, XY, r(13), de novo, with deletion points q32.2→qter. Our findings are in line with previous reports about chromosome 13 deletions, in which loss of the critical point leads to major malformations like brain anomalies and ambiguous genitalia.
Calcite 필러를 함유한 유리 복합체의 표면형상과 반사율
전재승,황성진,안지환,김형순,Jeon, Jae-Seung,Hwang, Seong-Jin,Ahn, Ji-Hwan,Kim, Hyung-Sun 한국재료학회 2009 한국재료학회지 Vol.19 No.8
Reflection properties, such as specular reflection and diffuse reflection, are very important optical properties for the reflector, which has high reflectance in the display and architecture industry. Calcite is lowcost, nontoxic, and stable over a wide temperature range. Therefore, it is one of the most widely using fillers in many industries and has some advantages over titania as a filler to improve reflectance. However, optical properties, especially those of ceramic-filled composites, have not been analyzed. We studied the reflectance of calcite composites with their surface roughness. The reflectance of the composites was determined using a UV-visible spectrometer. The surface morphology and the micro-structure of the composites were investigated by atomic force microscope. The reflectance of the composites was improved by increasing the content of calcite in the calcite-frit composite. The reflectance is related with the surface roughness in the composites. However, the reflectance depends on the calcite contents in materials with similar surface roughness.
조현준(Hyeon-Jun Cho),황성진(Sung-Jin Hwang),이재윤(Jea-Yun Lee),김승구(Seung-Ku Kim),박경환(Kyung-Hwan Park) 한국정보과학회 2004 한국정보과학회 학술발표논문집 Vol.31 No.2Ⅲ
기존 항공물류 산업의 시스템들은 많은 이질적인 시스템들과 비효율적이고 비표준화된 데이터로 이루어져 있다. 이러한 데이터의 통합문제를 해결하기 위한 해결방안이 많이 제시되고 있으며, XML 웹서비스 또한 해결책으로 제시되었다. 본 논문에서는 XML 웹서비스를 항공물류 산업에 적용시켜 항공물류 시스템을 통합하는 방법을 제시하고 현업에서 사용 가능한 XML 웹서비스 기반의 항공물류 공급망 시스템을 개발하였다.
자궁경부암 치료에서 p53 종양억제유전자의 플라스미드와 아데노바이러스를 이용한 유전자 치료법의 개발
이준모(Jun Mo Lee),김승조(Seung Jo Kim),남궁성은(Sung Eun NamKoong),조성대(Sung Dae Cho),황성진(Seong Jin Hwang),박현라(Hyun Ra Park),한유진(You Jin Han),김상태(Sang Tae Kim),이헌영(Hun Young Lee),김동재(Dong Jae Kim),박용석(Yong Serk 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.9
연구배경: 악성종양의 치료법에서 근간을 이루고 있는 방법은 수술, 방사선과 항암요법이다. 이와같은 치료에도 불구하고 종양세포의 파괴를 주 목표로 하고 있는 현재의 치료법은 그 한계에 도달하고 있다. 따라서 앞으로 종양치료의 방향은 이미 언급한 고식적인 방법과 아울러 종양에 대한 인체의 면역 반응을 항진시켜 종양세포 손상을 유도하는 유전자 치료쪽으로 연구가 진행되어야 할 것으로 사료되어 연구를 시작하였다. 연구목적: 자궁경부암의 유전자 치료를 위하여 자궁경부암 세포주들에 리포솜을 이용한 종양억제 유전자의 직접 이입법 (pRcCMVp53/ lipofectin)과 아데노 바이러스 (AdCMVp53)를 이용한 간접이입법에 의한 종양세포의 성장억제효과를 보고자 하였다. 연구방법: 이 연구에 사용한 자궁경부암 세포주들은 억제 유전자인 야생형 p53유전자를 가지고 있고 인유두종 바이러스 (human papilloma virus : HPV) 16 양성을 나타내는 CaSki, SiHa 세포주, HPV 18 양성을 나타내는 HeLa, HeLaS3 세포주와 HPV 음성을 나타내는 C33A, HT3 세포주이었다. LacZ를 표지유전자로 사용하여 그 이입효율을 확인 하였으며 인체 자궁경부암세포주로의 유전자의 직접 이입법으로는 lipofectin을 매개로 한 방법(pRcCMVp53/lipofectin)을 사용하였고 간접이입법으로는 바이러스를 사용한 AdCMVp53를 사용하였다. 암세포 성장억제 효과는 세포수 측정을 통해 확인하였다. 결과: p53 유전자의 이입에의한 자궁경부암 세포주의 성장억제는 직접이입법에의한 (pRcCMVp53/lipofectin) 경우 CaSki에서는 88.5%, SiHa는 59.1%, HeLa는86.0%, HeLaS3는 78.0%, C33A는 91.3%, 그리고 HT3에서는 74.0%의 성장억제를 나타냈다. 간접이입법 (AdCMVp53)에 의한 세포수 측정에서 CaSki인 경우에는 97.4%, SiHa는 91.6%, HeLa는 95.8%, HeLaS3는 99.7%, C33A는 97.3%, 그리고 HT3에서는 87.4%의 성장억제를 나타냈다. 결론: 자궁경부암 세포주에 직, 간접 이입법을 통한 억제유전자 전달 후 세포성장 억제는 HPV 양, 음성 모든세포주에서 매우 유의하게 감소되었으며 CaSki, HeLa세포주인 경우 직·간접 이입법에서는 유의한 차이가 없었으나 SiHa, HeLaS3, C33A세포주에서는 간접법 HT3세포주에서는 직접법이 좋은 것으로 나타났다. 이와같은 결과는 향후 자궁경부암 환자에 있어서 억제유전자의 직·간접이입법에 의한 유전자 치료를 실시할 수 있는 새로운 장으로 그 의의가 클것으로 생각 되어진다. Background: The basic treatment of malignant tumors is surgery, radiotherapy, chemotherapy. Even though, the object of these treatments is to kill cancer cells, they have limitations. So, in future studies of treatment of cancer, we should look into increasing human immune response using gene therapy in order to induce damage to tumor cells. Objective: The cell growth inhibitory effect of cervical cancer cells was investigated by direct transfection using liposome(pRcCMVp53/lipofectin). and by indirect transfection using Adenovirus(AdCMVp53). Methods: The cervical cancer cell lines we used in this study were HPV16 positive, having inhibitory gene, wild p53 gene, CaSki, SiHa, HPV18 positive HeLa, HeLaS3 and HPV negative C33A, HT3, LacZ gene was used as the marker gene for the transfection efficacy. Direct transfection was done by using lipofectin (pRcCMVp53/lipofectin) and indirect transfection was done by using virus, AdCMVp53. The effect of tumor cell growth inhibition was measured by cell counting assay. Result: Inhibition of growth of cervical cancer cells in cell counts of direct transfection was CaSki(88.5%), SiHa(59.1%), HeLa(86.0%), HeLaS3(78.0%), C33A(91.3%) and HT3(74.0%). Inhibition of growth of cervical cancer cells in cell counts of indirect transfection was CaSki(97.4%), SiHa(91.6%), HeLa(95.8%), HeLaS3(99.7%), C33A(97.3%) and HT3(87.4%). Conclusion : The inhibition of cell growth of cervical cancer cells by direct and indirect transfection was significantly reduced, and showed little differences depending on the type of cells. These results will have a great meaning in treating cervical cancer patients using gene therapy by direct or indirect transfection