http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 허재봉 (검색결과 9 건)
- 무료
- 기관 내 무료
- 유료
-
-
임동윤,허재봉,박유환,Lim Dong-Yoon,Heo Jae-Bong,Park Yoo Han The Korean Society of Pharmacology 1995 대한약리학잡지 Vol.31 No.1
저산소 상태에서는 부신수질로부터 카테콜아민 (CA) 유리작용이 활성화되지만 반면에 소의 배양 chromaffin cell에서는 고통도의 $K^+$에 의한 CA 분비작용이 억제된다고 알려져 있다. 본 연구에서는 적출 흰쥐 관류부신에서 콜린성 자극과 막탈분극에 의한 CA 분비작용에 대한 저산소증의 영향을 검색하고 그 작용기전을 규명코자 하였다. 본 연구목적을 위하여, 적출 흰쥐 관류부신을 이용, 저산소증이 니코틴($N_1$), 무스카린($M_1$) 수용체 흥분약, 막탈분극 약물, 칼슘채널 활성화 약물, 세포내 칼슘유리 약물 및 ACh에 의한 CA 분비에 미치는 영향을 연구하였으며, 저산소증은 95% 질소 및 5% 이산화탄소 혼합가스를 Krebs액에 주입하여 유발시켰으며, $3{\sim}4$시간동안 유지하였다. 저산소증 유발시, DMPP ($100{\mu}M$), McN-A-343 ($100{\mu}M$), ACh (5.32 mM), Bay-K-8644 ($10{\mu}M$) 및 high $K^+$ (56 mM)에 의한 CA 분비작용을 시간의존적으로 점차 유의성인 감소를 나타내었다. 그러나, cyclopiazonic acid ($10{\mu}M$)에 의한 CA 분비반응에는 하등의 영향을 일으키지 못하였다. 또한 저산소증 자체가 CA의 기초분비 작용에는 영향을 미치지 않았다. 이와같은 실험결과로 보아, 저산소증시 콜린성 자극 및 막탈분극에 의한 CA 분비 작용이 억제되며, 이러한 억제작용은 chromaffin cell내로 $Ca^{++}$ 유입을 직접적으로 억제시키는 결과에 기인되며, 세포내 칼슘저장고로부터 칼슘유리작용과는 관계없는 것으로 사료된다. It has been known that, during hypoxia, the adrenal medulla is activated to release catecholamines (CA) while hypoxia also inhibits high $K^+$ -induced CA secretion in the cultured bovine adrenal chromaffin cells. The present study was attempted to examine the effect of hypoxia on CA secretion evoked by chlinergic stimulation and membrane-depolarization from the isolated perfused rat adrenal glands and also to clarify its mechanism of action. For this purpose, using the isolated rat adrenal glands, the effects of hypoxia on CA release evoked by nicotinic ($N_1$) and muscarinic ($M_1$) receptor agonists, membrane-depolarizing agent, $Ca^{++}$-channel activator, intracellular $Ca^{++}$-releaser and ACh were determined. Experiments were carried out, perfusing Krebs solution pre-equilibrated with a gas mixture of 95% N_2$ and 5% $CO_2$. Hypoxia was maintained for $3{\sim}4$ hours through the experiments. Hypoxia gradually caused a time-dependent seduction in CA secretion evoked by DMPP ($100{\mu}M$), McN-A-343 ($100{\mu}M$), ACh (5.32 mM), Bay-K-8644 ($10{\mu}M$) and high $K^+$ (56 mM) respectively. How-ever, it did not affect CA secretion evoked by cyclopiazonic acid ($10{\mu}M$). Hypoxia itself also did fail to produce any influence on spontaneous secretory response of CA. These experimental results suggest that hypoxia depresses CA release evoked by both cholinergic stimulation and membrane-depolarization from the isolated rat adrenal medulla, and that this inhibitory activity may be due to the result of the direct inhibition of $Ca^{++}$ influx into the chromaffin cells without any effect on the calcium mobilization from the intracellular store.
-
The Mode of Action of Pentazocine on Catecholamine Secretion from the Perfused Rat Adrenal Medulla
임동윤,김봉한,허재봉,최철희,김진호,장영,이재준,Lim, Dong-Yoon,Kim, Bong-Han,Heo, Jae-Bong,Choi, Cheo-Hee,Kim, Jin-Ho,Jang, Young,Lee, Jae-Joon The Korean Society of Pharmacology 1994 대한약리학잡지 Vol.30 No.3
Pentazocine은 opioid 수용체에 대한 흥분작용과 길항작용을 겸유한 opioid계 약물로 알려져 있다. 본 연구에서 흰쥐 적출 관류부신으로 부터 pentazocine의 catecholamine(CA) 분비작용을 관찰하여 그 기전을 규명하고 또한 다른 opioid의 작용과 비교하여 얻어진 결과는 다음과 같다. Pentazocine$(30{\sim}300\;ug)$을 부신정맥내에 주사하였을때 현저한 용량의 존성의 CA 분비작용을 나타내었다. Pentazocine의 이러한 CA 분비작용은 chlorisondamine $(10^{-6}\;M)$, naloxone $(1.22{\times}10^{-7}\;M)$, morphine $(1.73{\times}10^{-5}\;M)$, enkephalin $(9.68{\times}10^{-6}\;M)$, nicardipine $(10^{-6}\;M)$ 및 TMB-8$(10^{-5}\;M)$등의 전처치로 뚜렷이 억제되었으나 pirenzepine (2과$10^{-6}\;M)$의 전처치에 의해서는 영향을 받지 않았다. $Ca^{++}$-free Krebs 용액으로 30분간 관류한 후에 pentazocine의 CA 분비작용은 현저한 감소를 나타냈었다. Pentazocine $(1.75{\times}10^{-4}\;M)$을 20분간 관류시킨 후에 ACh $(5.32{\times}10^{-3}\;M)$과 DMPP $(10^{-4}\;M)$에 의한 CA 분비작용이 의의 있게 감약되었다. 이상과 같은 연구결과를 종합하면, pentazocine은 횐쥐 적출 관류부신에 투여시 현저한 CA 분비작용을 일으키고 있는 칼슘의존성 exocytotic mechanism에 의한 것으로 생각되며, 이러한 pentazocine의 CA 분비작용은 부신 chromaffin cell에 있는 opioid 수용체의 활성화를 통하여 나타나며, 또한 부신의 nicotine 수용체의 흥분작용과도 관련성이 있는 것으로 사료된다. The present study was attempted to investigate whether pentazocine, which is known to possess both opioid agonistic and antagonistic properties, produces catecholamines (CA) secretion from the isolated perfused rat adrenal gland, and to establish the mechanism of its action, and also to compare its action with that of some opioids. Pentazocine (30 to 300 ug) injected into an adrenal vein caused a dose-dependent secretory response of CA from the rat adrenal medulla. The pentazocine-evoked secretion of CA was remarkably diminished by the preloading with chlorisondamine $(10^{-6}\;M)$, naloxone $(1.22{\times}10^{-7}\;M)$, morphine $(1.7{\times}10^{-5}\;M)$, met-enkephalin $(9.68{\times}10^{-6}\;M)$, nicardipine $(10^{-6}\;M)$ and TMB-8 $(10^{-5}\;M)$ while was not influenced by the pretreatment of pirenzepine $(2{\times}10^{-6}\;M)$. The perfusion of $Ca^{++}$-free Krebs solution for 30 min into the gland also led to the marked reduction in CA secretion evoked by pentazocine. Furthermore, the CA release evoked by ACh and/or DMPP was greatly inhibited by the pretreatment with pentazocine $(1.75{\times}10^{-4}\;M)$ for 20 min. From these experimental results, it is thought that pentazocine causes markedly the increased secretion of CA from the isolated perfused rat adrenal medulla by a calcium-dependent exocytotic mechanism. The secretory effect of pentazocine appears to be mediated through activation of opioid receptors located on adrenal chromaffin cells, which may be also associated with stimulation of cholinergic nicotinic receptors.
-
김태원,조은택,조기섭,정재용,허재봉,장영,박유환,정춘해 조선대학교 1995 The Medical Journal of Chosun University Vol.20 No.1
A retrospective clinical and histologic study of 30 non-Hodgkin's lymphomas diagnosed at the Department of Hemato-oncology of the Chosun University from January 1989 to December 1993 is presented. Among total 30 cases whose sexes were mentioned, male were 18 and female were 12 and the ratio is 1.5:1. The peak incidence of non-Hodgkin's lymphoma was found in the third decade. The case distributions by Working formulation were summarized in table 3. The most common histopathologic type of non-Hodgkin's lymphoma was the diffuse large cell type of intermediate grade. The presenting symptoms at the time of first visit to the hospital were summarized in table 4. The most common symptoms were palpable mass. Lymph node enlargements were found in 87 %, and most frequently on cervical area(35%). The incidence of primary nodal lymphoma is more frequent than extranodal lymphoma, 1.2:1. The most common primary site of extranodal lymphoma is the gastrointestinal tract. In the laboratory findings, the data of hemoglobin and hematocrit were summarized in table 8, 9, Values of hemoglobin and hematocrit of female patients greater than those of male patients. The results of the immunophenotypings of nodal and extranodal lymphoma indicates that the B-cell lymphoma are predominant(B:T=1.4:1). The clinical stage was Ⅱ in 9 cases(30%) Ⅲ in 9 cases(30%), Ⅳ in 8 cases(26.7%), Ⅰin 4 cases(13.3%), 28 cases were treated with CHOP regimen. Among them, 17 cases(60.8%) achieved a complete remission. 5 cases had a partial response. The most frequent toxicities were toxic hepatitis.
-
저산소증이 흰쥐 관류부신에서 DMPP, McN-A-343, Excess K<sup>+</sup> 및 Ach의 카테콜아민 분비작용에 미치는 영향
임동윤(Dong-Yoon Lim),허재봉(Jae-Bong Heo),박유환(Yoo-Han Park) 대한약리학회 1995 대한약리학잡지 Vol.31 No.1
저산소 상태에서는 부신수질로부터 카테콜아민 (CA) 유리작용이 활성화되지만 반면에 소의 배양 chromaffin cell에서는 고통도의 K<sup>+</sup>에 의한 CA 분비작용이 억제된다고 알려져 있다. 본 연구에서는 적출 흰쥐 관류부신에서 콜린성 자극과 막탈분극에 의한 CA 분비작용에 대한 저산소증의 영향을 검색하고 그 작용기전을 규명코자 하였다. 본 연구목적을 위하여, 적출 흰쥐 관류부신을 이용, 저산소증이 니코틴(N<sub>1</sub>), 무스카린(M<sub>1</sub>) 수용체 흥분약, 막탈분극 약물, 칼슘채널 활성화 약물, 세포내 칼슘유리 약물 및 ACh에 의한 CA 분비에 미치는 영향을 연구하였으며, 저산소증은 95% 질소 및 5% 이산화탄소 혼합가스를 Krebs액에 주입하여 유발시켰으며, 3 ~ 4시간동안 유지하였다. 저산소증 유발시, DMPP (100μM), McN-A-343 (100μM), ACh (5.32 mM), Bay-K-8644 (10μM) 및 high K<sup>+</sup> (56 mM)에 의한 CA 분비작용을 시간의존적으로 점차 유의성인 감소를 나타내었다. 그러나, cyclopiazonic acid (10μM)에 의한 CA 분비반응에는 하등의 영향을 일으키지 못하였다. 또한 저산소증 자체가 CA의 기초분비 작용에는 영향을 미치지 않았다. 이와같은 실험결과로 보아, 저산소증시 콜린성 자극 및 막탈분극에 의한 CA 분비 작용이 억제되며, 이러한 억제작용은 chromaffin cell내로 Ca<sup>++</sup> 유입을 직접적으로 억제시키는 결과에 기인되며, 세포내 칼슘저장고로부터 칼슘유리작용과는 관계없는 것으로 사료된다. It has been known that, during hypoxia, the adrenal medulla is activated to release catecholamines (CA) while hypoxia also inhibits high K<sup>+</sup> -induced CA secretion in the cultured bovine adrenal chromaffin cells. The present study was attempted to examine the effect of hypoxia on CA secretion evoked by chlinergic stimulation and membrane-depolarization from the isolated perfused rat adrenal glands and also to clarify its mechanism of action. For this purpose, using the isolated rat adrenal glands, the effects of hypoxia on CA release evoked by nicotinic (N<sub>1</sub>) and muscarinic (M<sub>1</sub>) receptor agonists, membrane-depolarizing agent, Ca<sup>++</sup>-channel activator, intracellular Ca<sup>++</sup>-releaser and ACh were determined. Experiments were carried out, perfusing Krebs solution pre-equilibrated with a gas mixture of 95% N<sub>2</sub> and 5% CO<sub>2</sub>. Hypoxia was maintained for 3 ~ 4 hours through the experiments. Hypoxia gradually caused a time-dependent seduction in CA secretion evoked by DMPP (100μM), McN-A-343 (100μM), ACh (5.32 mM), Bay-K-8644 (10μM) and high K<sup>+</sup> (56 mM) respectively. How-ever, it did not affect CA secretion evoked by cyclopiazonic acid (10μM). Hypoxia itself also did fail to produce any influence on spontaneous secretory response of CA. These experimental results suggest that hypoxia depresses CA release evoked by both cholinergic stimulation and membrane-depolarization from the isolated rat adrenal medulla, and that this inhibitory activity may be due to the result of the direct inhibition of Ca<sup>++</sup> influx into the chromaffin cells without any effect on the calcium mobilization from the intracellular store.
-
-
-
-
흰쥐 관류부신에서 Pentazocine의 카테콜아민 분비작용의 기전
임동윤(Dong-Yoon Lim),김봉한(Bong-Han Kim),허재봉(Jae-Bong Heo),최철희(Cheo-Hee Choi),김진호(Jin-Ho Kim),장영(Young Jang),이재준(Jae-Joon Lee) 대한약리학회 1994 대한약리학잡지 Vol.30 No.3
Pentazocine은 opioid 수용체에 대한 흥분작용과 길항작용을 겸유한 opioid계 약물로 알려져 있다. 본 연구에서 흰쥐 적출 관류부신으로 부터 pentazocine의 catecholamine(CA) 분비작용을 관찰하여 그 기전을 규명하고 또한 다른 opioid의 작용과 비교하여 얻어진 결과는 다음과 같다. Pentazocine(30 ~ 300 ug)을 부신정맥내에 주사하였을때 현저한 용량의 존성의 CA 분비작용을 나타내었다. Pentazocine의 이러한 CA 분비작용은 chlorisondamine (10<sup>-6</sup> M), naloxone (1.22 × 10<sup>-7</sup> M), morphine (1.73 × 10<sup>-5</sup> M), enkephalin (9.68 × 10<sup>-6</sup> M), nicardipine (10<sup>-6</sup> M) 및 TMB-8(10<sup>-5</sup> M)등의 전처치로 뚜렷이 억제되었으나 pirenzepine (2과 10<sup>-6</sup> M)의 전처치에 의해서는 영향을 받지 않았다. Ca<sup>++</sup>-free Krebs 용액으로 30분간 관류한 후에 pentazocine의 CA 분비작용은 현저한 감소를 나타냈었다. Pentazocine (1.75 × 10<sup>-4</sup> M)을 20분간 관류시킨 후에 ACh (5.32 × 10<sup>-3</sup> M)과 DMPP (10<sup>-4</sup> M)에 의한 CA 분비작용이 의의 있게 감약되었다. 이상과 같은 연구결과를 종합하면, pentazocine은 횐쥐 적출 관류부신에 투여시 현저한 CA 분비작용을 일으키고 있는 칼슘의존성 exocytotic mechanism에 의한 것으로 생각되며, 이러한 pentazocine의 CA 분비작용은 부신 chromaffin cell에 있는 opioid 수용체의 활성화를 통하여 나타나며, 또한 부신의 nicotine 수용체의 흥분작용과도 관련성이 있는 것으로 사료된다. The present study was attempted to investigate whether pentazocine, which is known to possess both opioid agonistic and antagonistic properties, produces catecholamines (CA) secretion from the isolated perfused rat adrenal gland, and to establish the mechanism of its action, and also to compare its action with that of some opioids. Pentazocine (30 to 300 ug) injected into an adrenal vein caused a dose-dependent secretory response of CA from the rat adrenal medulla. The pentazocine-evoked secretion of CA was remarkably diminished by the preloading with chlorisondamine (10<sup>-6</sup> M), naloxone (1.22 × 10<sup>-7</sup> M), morphine (1.7 × 10<sup>-5</sup> M), met-enkephalin (9.68 × 10<sup>-6</sup> M), nicardipine (10<sup>-6</sup> M) and TMB-8 (10<sup>-5</sup> M) while was not influenced by the pretreatment of pirenzepine (2 × 10<sup>-6</sup> M). The perfusion of Ca<sup>++</sup>-free Krebs solution for 30 min into the gland also led to the marked reduction in CA secretion evoked by pentazocine. Furthermore, the CA release evoked by ACh and/or DMPP was greatly inhibited by the pretreatment with pentazocine (1.75 × 10<sup>-4</sup> M) for 20 min. From these experimental results, it is thought that pentazocine causes markedly the increased secretion of CA from the isolated perfused rat adrenal medulla by a calcium-dependent exocytotic mechanism. The secretory effect of pentazocine appears to be mediated through activation of opioid receptors located on adrenal chromaffin cells, which may be also associated with stimulation of cholinergic nicotinic receptors.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
