알톨 대사에 미치는 인삼의 영향

허근,최종원,Huh, Keun,Choi, Chong-Won 대한약학회 1984 약학회지 Vol.28 No.1

After pretreatment with ginseng followed by induction of acute intoxication of alcohol, the activities of alcohol dehydrogenase (ADH), microsomal ethanol-oxidizing system (MEOS) and aldehyde dehydrogenase(Ald DH) increased respectively compared to the groups treated with alcohol alone. In case that ginseng was given to rats fed with 5% alcohol instead of water for 60 days, the activities of ADH and MEOS increased compared to the groups treated. On the contrary, the activity of Ald DH in mitochondrial fraction decreased to an extent of about 35% in chronic alcoholism, but after pretreatment of ginseng the activity was restored to the control level. On the other hand, the catalase activity was not significantly affected by either treatment. Ginseng butanol fraction significantly increased the serum isocitrate dehydrogenase activity which is inhibited by alcohol-treated in rat. Alcohol-induced lactate dehydrogenase activity was decreased to control level in liver by ginseng treatment. And the serum level of lactic acid also decreased by ginseng treatment in alcohol-intoxicated rat. Ginseng butanol fraction markedly decreased the xanthine oxidase activity in the ethanol-treated rat liver. It was also observed that ginseng reduced the blood concentration of uric acid on experimentally reduced hyperuricemia by alcohol treatment. Uricase activity was not affected by either treatment. Ginseng butanol fraction decreased the hepatic aniline hydroxylase activity which was induced by alcohol-treated rat. These results suggest that the treatment with ginseng can be promoted the recovery from alcohol intoxication and some therapeutic effect on alcoholinduced metabolic disease.

Effect of Panax Ginseng in Mouse Liver with Ethnol-Induced Monoamine Oxidase Activity

허근,이상일,송민익,신억섭,박종민,Huh, Keun,Lee, Sang-Il,Song, Min-Ik,Shin, Uk-Seob,Park, Jong-Min The Korean Society of Pharmacology 1989 대한약리학잡지 Vol.25 No.1

본 실험에서는 ethanol 및 acetaldehyde가 유도하는 MAO 활성변동에 인삼 butanol 분획이 미치는 영향을 검토하였다. Ethanol(1 g/kg)을 mouse 복강내로 투여하였을 때 간장 중 MAO 활성이 현저히 증가하였으며 이러한 작용은 인삼butanol 분획 전처리로 개선되었다. 또한 pyrazole(200mg/kg)을 전처리하였을 때 ethanol에 의해 증가된 간장중 MAO활성이 정상수준으로 감소되었다. 만성적으로 ethanol을 투여한 경우에 있어서도 MAO활성이 증가되었으며 인삼 butaonl분획 전처리로 MAO 활성이 감소됨이 관찰되었다. Ethanol의 대사산물인 acetaldehyde(100mg/kg)을 복강내로 투여하고 관찰한 실험에서도 간장중 MAO활성이 현저하게 증가되었으며 인삼 butanol분획을 전처리함으로써 정상수준 가깝게 MAO활성이 감소되었다. 이 연구결과로 ethanol에 의한 간장중 MAO활성증가는 ethanol의 대사산물인 acetaldehyde에 기인된 것으로 생각되어지며 인삼 butanol분획이 acetaldehyde에 의해 유도되는 간장중 MAO활성 변동을 조절하고 있을 것으로 사료된다. Effect of ginseng butanol fractions on the hepatic mitochondrial monoamine oxidase activity with ethanol treatment was investigated in this experiment. Ethanol treatment, either acutely or chronically, increased the hepatic mitochondrial monoamine oxidase activity compared to control group. Whereas, treatment with ginseng butanol fractions lowered the ethaol-induced monoamine oxidase activity. Acetaldehyde, the major metabolite of ethanol, significantly increased the hepatic mitochondrial monoamine oxidase activity more than ethanol did. It was also observed that ginseng butanol fractions reduced the increase of the hepatic mitochondial monoamine oxidase activity by acetaldehyde. From these results, it is suggested that ginseng butanol fractions may be associated with the modulation of the hepatic mitochondrial monoamine oxidase activity in ethanol-treated animals.

마늘성분이 간 Xanthine Oxidase 활성변화에 미치는 영향

허근,이상일,박종민 ( Keun Huh,Sang Il Lee,Jong Min Park ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.3

It was attempted to observe the effect of garlic on the hepatic xanthine oxidase activity in this study. Xanthine oxidase which catalyze the oxidation of many purine and pyrimidine derivatives was further purified by centrifugation, heating, (NH₄)₂SO₄fraction, acetone fraction and dialysis, hydroxyapatite column. Allicin and heat-treated allicin fraction powerfully inhibited xanthine oxidase activity in vitro. When the enzyme was incubated in reaction mixture containing garlic butanol fraction, it was found that xanthine oxidase activity was increased. Garlic bulbs and seeds ethanol extracts increased the enzyme activity, whereas leaves and roots extracts did not affected its activity in vitiro.

마늘성분이 간 aldehyde oxidase 활성변화에 미치는 영향

허근,이상일,박종민,김영문,김석환 ( Keun Huh,Sang Il Lee,Young Moon Kim,Jong Min Park Seok Hwan Kim ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.1

Effects of garlic components on the hepatic aldehyde oxidase activity were studied in this experiment. Aldehyde oxidase which catalyzes the oxidation of many purine, pyrimidine and pteridine derivatives was purified by centrifugation, heat treatment, ammonium sulfate fractionation, acetone fractionation and dialysis, and hydroxyapatite column chromatography. Allicin, heat-treated allicin and garlic butanol fraction powerfully inhibited aldehyde oxidase activity in vitro. It was also observed that aldehyde oxidase activity was inhibited in mice fed a 5% garlic juice in vivo.

Effects of Garlic on the Hepatic Aldehyde Oxidase in Mice and Rabbits

허근,이상일,박종민,김영문,김석환,Huh, Keun,Lee, Sang-Il,Kim, Young-Moon,Kim, Young-Moon,Kim, Seok-Hwan Korean Society for Biochemistry and Molecular Biol 1987 한국생화학회지 Vol.20 No.1

간 aldehyde oxidase 활성에 마늘 성분이 어떼한 영향을 주는가를 관찰할 목적으로 in vivo 및 in vitro 실험을 하였다. 마늘 수침액을 mouse에 섭취케 하였을때 간 aldehyde oxidase의 활성은 유의성 있게 감소하였다. 이와같은 마늘성분에의한 aldehyde oxidase 활성억제작용이 어떤 성분에 기인된 것인지를 확인하기 위하여 마늘로부터 추출한 allicin분획과 allicin을 열분해시킨 분획 및 butanol분획을 이용하여 토끼간으로부터 정제한 aldehyde oxidase의 활성을 비교 관찰 하였을때 3분획 모두 본 효소의 활성을 현저히 억제시켰으며 이와 같은 억제작용은 열처리한 allicin분획에서 더 강하게 나타났다. 한편 본 효소와 기질간의 반응속도에 마늘성분이 어떠한 영향을 미치는가를 검토하였을때 상기 3분획 모두 $V_{max}$에는 영향이 없었으나 $K_m$치를 증가시키는 경쟁적 저해현상을 나타내었다. Effects of garlic components on the hepatic aldehyde oxidase activity were studied in this experiment. Aldehyde oxidase which catalyzes the oxidation of many purine, pyrimidine and pteridine derivatives was purified by centrifugation, heat treatment, ammonium sulfate fractionation, acetone fractionation and dialysis, and hydroxyapatite column chromatography. Allicin, heat-treated allicin and garlic butanol fraction powerfully inhibited aldehyde oxidase activity in vitro. It was also observed that aldehyde oxidase activity was inhibited in mice fed a 5% garlic juice in vivo.

위성통신용 Ku-Band 믹서에 관한 연구

허근,류연국,홍의석,Her, Keun,Ryou, Yeon-Guk,Hong, Ui-Seok 한국통신학회 1993 韓國通信學會論文誌 Vol.18 No.6

본 논문은 마이크로웨이브 시뮬레이션 CAD, LINMIC+을 이용하여 소신호 산란계수로 Ku-band용 FET 주파수혼합기를 설계 및 구현하였다. 입력주파수 14GHz에서 구현한 주파수혼합기의 성능을 국부발진 전력레벨이 +1dBm일때 중간주파수 1GHz에서 9.88dB의 변환이득을 얻었다. 또 중간주파수 1.1GHz에서 최대변환이득 11.71dB를 얻었다. 결국 신호주파수 증폭기나 중간주파수 증폭기의 필요성이 낮아진다. 또 낮은 국부발진 전력레벨로 원하는 변환이득을 유지하면서 주파수의 혼합이 가능함을 입증하였다. 변환이득(conversion gain)은 가용이득보다 큰 것을 실험적으로 확인하였다. In this paper a FET mixer is designed realized by small-signal S-parameter using microwave CAD, LINMIC + at Ku-band. The mixer has conversion gain 9.88dB at 14GHz RF, 1GHz IF, and + 1dBm LO imput. The maximum conversion gain is obtained 11.71dB at 1.1GHz. The result shows that the FET mixer does not need pre-and/or IF amplifier. The mixer maintains the desired conversion gain with low LO power level. The conversion gain of the mixer is higher than the available gain of a amplifier, which is experimentally verified.

Metallothionein이 지질과산화반응과 Aldehyde Oxidase활성에 미치는 영향

허근,신억섭,박종민,Huh, Keun,Shin, Uk-Seob,Park, Jong-Min 한국독성학회 1995 Toxicological Research Vol.11 No.1

The effects of metallothionein and cadmium ion on the hepatic aldehyde oxidase activity and brain lipid peroxidation were tested in vitro. The content of brain lipid peroxide at the condition of normal or $300{\mu}M$ Fe(II)-induced was remarkably reduced by the addition of metallothionein in the incubation mixture. The induced content of lipid peroxide by cadmium $(30{\mu}g/ml)$ ion was reduced by metallothionein $(100{\mu}g/ml)$. The activity of aldehyde oxidase was not affected by metallothionein, but cadmium ion $(8.38{\mu}g/ml)$ increased the activity of aldehyde oxidase about 80% compared to the control. The cadmium-induced activity of aldehyde oxidase was restored to the control level by metallothionein or penicillamine.

Pyrazinamide와 그 대사산물이 유발하는 고뇨산 혈증에 미치는 Pyridoxal의 영향

허근,박종민,신억섭,김석환,Huh, Keun,Park, Jong-Min,Shin, Uk-Seob,Kim, Suk-Hwan 대한약학회 1987 약학회지 Vol.31 No.3

Pyrazinamide, an amide of pyrazinoic acid, is widely used in combination with other drugs for the treatment of tuberculosis. It was attempted to observe the effect of pyridoxal on the pyrazinamide induce hyperuricemia in this study. It was observed that the values of serum transminases were not changed in mice injected pyrazinamide and pyrazinoic acid, respectively (100, 200, and 300mg/kg) compared with control. Blood urate levels were increased in mice treated with these drugs in a dose dependent manner. After pyrazinoic acid was administered intraperitoneally at a dose of 300mg/kg pretreated with 50mg/kg of pyridoxal once daily for 4 days, the blood levels of uric acid and pyrazinoic acid were decreased significantly.

마우스의 아세트알데히드 대사에 미치는 인삼 부탄올 분획의 영향

허근,박종민,이상일,최종원,Huh, Keun,Park, Chong-Min,Lee, Sang-Il,Choi, Chong-Won 대한약학회 1985 약학회지 Vol.29 No.1

The present study was undertaken to investigate the possible effect of ginseng butanol fraction on the hepatic acetaldehyde metabolism. Experimental animals were used for the subject of the study. When, in case of mitochondrial aldehyde dehydrogenase (Ald DH), ginseng butanol fraction was added, enzyme activity was increased in a small dose, while, in a large dose, it showed inhibitory effect. In terms of kinetic aspect, ginseng butanol fraction has the effect to decrease the Km values of Ald DH. In vivo studies, the activity of Aid DH increased by induction of acute intoxication of ethanol was further increased through pretreatment with ginseng butanol fraction. When ginseng butanol fraction was given to mice fed with 5% ethanol instead of water for 60 days, the activity of Ald DH in mitochondrial fraction decreased to about 35% in chronic alcoholism, but after pretreatment of ginseng butanol fraction the activity was restored to the control level. By the pretreatment with disulfiram, the Ald DH activity was inhibited in normal and alcohol-treated groups, but after the treatment with ginseng butanol fraction the activity was restored to the control level. The results suggest that ginseng butanol fraction enhance the Ald DH activity inhibited by the treatment of disulfiram with no relation to NAD. It was observed that ginseng butanol fraction markedly decrease the acetaldehyde levels in plasma and liver. All these observations suggested that reduction of acetaldehyde in blood and liver should be dependent upon increased activity of mitochonclrial Ald DH. It is concluded that the recovery from alcohol intoxication should be prompted by treatment with ginseng.

Effect of Sinigrin on the Hepatic Xanthine Oxidase and Free Radical Scavenging Enzyme Activities in Mice

허근,이상일,송민익,Huh, Keun,Lee, Sang-Il,Song, Min-Ik Korean Society for Biochemistry and Molecular Biol 1991 한국생화학회지 Vol.24 No.1

Ethanol을 투여한 mouse 간에서 free radical 생성효소인 xanthine oxidase와 분해효소 활성에 미치는 sinigrin의 영향을 관찰하였다. Ethanol 투여에 의해 간 xanthine oxidase 활성은 대조군에 비해 증가하였으나 $NAD^+$ 의존성 xanthine dehydrogenase 활성은 감소하였다. 한편 sinigrin의 전처리는 ethanol 투여에 의해 증가되는 간 xanthine oxidase의 활성 및 조직 중의 과산화지질 함량을 감소시켰다. Free radical 분해효소인 superoxide dismutase와 catalase 활성을 관찰한 실험에서 sinigrin을 전처리한 실험군은 ethanol 단독 투여군에 비하여 유의적인 효소활성 변동을 나타나지 않았다. The effect of sinigrin was investigated on xanthine oxidase and free radical scavenging enzymes in ethanol-treated mice. In vivo, the treatment of ethanol increased the activity of the $O_2$-dependent hepatic xanthine oxidase compared to those of the control group. Whereas the activity of the $NAD^+$-dependent xanthine dehydrogenase was decreased about 50 percent. The pretreatmemt of sinigrin reduced the increment of the activity of xanthine oxidase and hepatic lipid peroxide content in ethanol-treated mice. On the superoxide scavenging system, the superoxide dismutase activity with the treatment of ethanol showed the trend of increasing, whereas the sinigrin pretreatment did not affect the enzyme activity. The activity of catalase which is another scavenger was not influenced by the ethanol or sinigrin treatment.