패랭이꽃 N-Butanol 추출물의 자궁수축작용에 관한 연구

허근(Keun Huh),류항목(Hang-Mook Ryu),이상일(Sang-Il Lee),박종민(Jong-Min Park),송재웅(Jae-Woong Song),신억섭(Uk-Seob Shin) 한국생약학회 1988 생약학회지 Vol.19 No.4

The present studies were investgated to find out the uterus contractive components and action mechanism of contractive components. We observed that the contractive components of Dianthi Herba were extracted with methanol and dissolved in butanol, The butanol extract of Dianthi Herba increased uterus contractility, and fraction 2 obtained from butanol extract was more powerful than other fractions. This action was not blocked by atropine, papaverine, prazosin, propranolol, chlorphe niramine, methysergide and diltiazem in vitro.

흰쥐 간 Glutathione peroxidase 활성에 미치는 Diallyl disulfide의 영향

허근(Keun Huh),이상일(Sang Il Lee),박종민(Jong Min Park) 대한약리학회 1986 대한약리학잡지 Vol.22 No.2

마늘성분중의 하나인 diallyl disulfide가 간 glutathione peroxidase 활성에 어떠한 영향을 주는가를 관찰하여 다음과 같은 성적을 얻었다. 여러 농도의 diallyl disulfide를 흰쥐에 투여 하였을때 혈청 transaminase의 활성은 변동이 없었으며 또한 간 glutathione의 함량도 변화되지 않았다. 그러나 간 가용성분획의 glutathion peroxidase 활성은 현저히 증가되었다. 시험관 내에서 간 가용성분획의 glutathione peroxidase의 활성은 15μg/㎖ 이상의 diallyl disulfide를 첨가 하였을때 현저히 증가되었으며, 동력학적인 측면에서 관찰하였을때 diallyl disulfide(20μg/㎖)에 의해 Km치는 별 변화가 없었으나 Vmax치는 현저히 증가되었다. 이상의 실험결과에서 마늘 성분 중에 glutathione peroxidase 활성을 증가시키는 성분은 allicin의 분해산물인 diallyl disulfide에 기인된 것으로 사료되며 또한 이 성분은 효소의 기질결합 부위가 아닌 다른 부위에서 효소의 활성을 조절해 줄 것으로 생각되어진다. Glutathione peroxidase might play an important role in the protection of cellular structures against oxidative challange by hydrogen peroxide and several organic hydroperoxides. It is widely accepted that allicin is biological active component of garlic, and allicin is easily degraded to diallyl disulfide and other components. This study was attempted to elucidate the effect of diallyl disulfide on some biological activities. It was observed that the activity of serum transaminase and glutathione level in liver were not changed by the treatment of diallyl disulfide. The liver cytosolic glutathione peroxidase activity was significantly enhanced. Whereas, mitochondrial enzyme activity was slightly increased. In the presence of diallyl disulfide in vitro, V_max value of glutathione peroxidase for hydrogen peroxide was increased. On the other hand, Km value was not changed.

Pyrazinamide와 그 대사산물이 유발하는 고뇨산 혈증에 미치는 Pyridoxal의 영향

허근,박종민,신억섭,김석환,Huh, Keun,Park, Jong-Min,Shin, Uk-Seob,Kim, Suk-Hwan 대한약학회 1987 약학회지 Vol.31 No.3

Pyrazinamide, an amide of pyrazinoic acid, is widely used in combination with other drugs for the treatment of tuberculosis. It was attempted to observe the effect of pyridoxal on the pyrazinamide induce hyperuricemia in this study. It was observed that the values of serum transminases were not changed in mice injected pyrazinamide and pyrazinoic acid, respectively (100, 200, and 300mg/kg) compared with control. Blood urate levels were increased in mice treated with these drugs in a dose dependent manner. After pyrazinoic acid was administered intraperitoneally at a dose of 300mg/kg pretreated with 50mg/kg of pyridoxal once daily for 4 days, the blood levels of uric acid and pyrazinoic acid were decreased significantly.

마우스의 아세트알데히드 대사에 미치는 인삼 부탄올 분획의 영향

허근,박종민,이상일,최종원,Huh, Keun,Park, Chong-Min,Lee, Sang-Il,Choi, Chong-Won 대한약학회 1985 약학회지 Vol.29 No.1

The present study was undertaken to investigate the possible effect of ginseng butanol fraction on the hepatic acetaldehyde metabolism. Experimental animals were used for the subject of the study. When, in case of mitochondrial aldehyde dehydrogenase (Ald DH), ginseng butanol fraction was added, enzyme activity was increased in a small dose, while, in a large dose, it showed inhibitory effect. In terms of kinetic aspect, ginseng butanol fraction has the effect to decrease the Km values of Ald DH. In vivo studies, the activity of Aid DH increased by induction of acute intoxication of ethanol was further increased through pretreatment with ginseng butanol fraction. When ginseng butanol fraction was given to mice fed with 5% ethanol instead of water for 60 days, the activity of Ald DH in mitochondrial fraction decreased to about 35% in chronic alcoholism, but after pretreatment of ginseng butanol fraction the activity was restored to the control level. By the pretreatment with disulfiram, the Ald DH activity was inhibited in normal and alcohol-treated groups, but after the treatment with ginseng butanol fraction the activity was restored to the control level. The results suggest that ginseng butanol fraction enhance the Ald DH activity inhibited by the treatment of disulfiram with no relation to NAD. It was observed that ginseng butanol fraction markedly decrease the acetaldehyde levels in plasma and liver. All these observations suggested that reduction of acetaldehyde in blood and liver should be dependent upon increased activity of mitochonclrial Ald DH. It is concluded that the recovery from alcohol intoxication should be prompted by treatment with ginseng.

흰쥐에서 에탄올이 유도한 간 Aniline Hydroxylase 활성에 미치는 인삼의 영향

허근(Keun Huh),이상일(Sang ll Lee),임상규(Sung Kyu Lim),최종원(Chong Won Choi),박종민(Chong Min Park) 영남대학교 자원문제연구소 1986 資源問題硏究 Vol.5 No.-

지질과산화 반응과 Free Radical 생성계 효소활성에 미치는 Testosterone의 영향

허근(Keun Huh),신억섭(Uk Seob Shin),박종민(Jong Min Park) 대한약학회 1994 약학회지 Vol.38 No.2

Sex hormones not only regulate external sexual characteristics but several internal biochemical processes. It is well accepted that life-span of female is longer than that of male. Life-span is closely related with aging process in which free radicals are known to be involved. We investigated the effect of testosterone on free radical generating systems and lipid peroxidation based on the sexual difference. Lipid peroxide levels of male and female mouse were increased proportionately with age, especially in male mouse. Increase in enzyme activity of aldehyde oxidase with age was observed in male mouse, while no siginificant change in enzyme activity was found in female mouse. Enzyme activity of xanthine oxidase also showed similar results. It, however, was not significant statistically. Lipid peroxide level and xanthine oxidase type conversion ratio of male and female mouse liver homogenate incubated at 37oC , increased remarkably in proportion to incubation time, especially in male mouse. Lipid peroxide level and aidehyde oxidase activity were measured in normal male mouse, castrated mouse and testosterone treated-castrated mouse. Castrated mouse group showed decrease in lipid peroxide level and aidehyde oxidase activity compared with normal group. Treatment of castrated mouse with testosterone, however turned the level of lipid peroxide and aldehyde oxidase activity back to normal. From the above results, it might be concluded that testosteron could increase the activities of free radical generating enzymes which would result in the formation of lipid peroxide, consequently leading to aging.

Purine 대사과정에 미치는 마늘 수침액의 영향

허근(Keun Huh),이상일(Sang Il Lee),박종민(Jong Min Park),김석환(Suk Hwan Kim) 대한약학회 1986 약학회지 Vol.30 No.2

It was attempted to observe the effect of garlic on the hepatic purine metabolizing enzymes in this study. The activities of adenosine deaminase, guanase, and uricase in rats were not changed significantly following the feeding of 5% garlic juice. Whereas, garlic juice inhibited significantly the hepatic xanthine oxidase activity compared to control group with the lapse of treated-period. The urate level of serum in rats was significantly decreased by the treatment of garlic juice. The above inhibitory effect of garlic was greater in boiled garlic juice than fresh garlic juice-treated group. These results indicated that, according to the chemical properties of allicin which is unstable in heat, other components than allicin in garlic may regulate the hepatic purine metabolizing enzymes.

Purine 대사과정에 미치는 마늘 수침액의 영향

허근(Keun Huh),이상일(Sang ll Lee),김석환(Seok Hwan Kim),박종민(Jong Min Park) 영남대학교 자원문제연구소 1987 資源問題硏究 Vol.6 No.-

Ethanol이 유도한 간장중 MAO 활성변동에 미치는 인삼의 영향

허근(Keun Huh),이상일(Sang-Il Lee),송민익(Min-Ik Song),신억섭(Uk-Seob Shin),박종민(Jong-Min Park) 대한약리학회 1989 대한약리학잡지 Vol.25 No.1

본 실험에서는 ethanol 및 acetaldehyde가 유도하는 MAO 활성변동에 인삼 butanol 분획이 미치는 영향을 검토하였다. Ethanol(1 g/kg)을 mouse 복강내로 투여하였을 때 간장 중 MAO 활성이 현저히 증가하였으며 이러한 작용은 인삼butanol 분획 전처리로 개선되었다. 또한 pyrazole(200mg/kg)을 전처리하였을 때 ethanol에 의해 증가된 간장중 MAO활성이 정상수준으로 감소되었다. 만성적으로 ethanol을 투여한 경우에 있어서도 MAO활성이 증가되었으며 인삼 butaonl분획 전처리로 MAO 활성이 감소됨이 관찰되었다. Ethanol의 대사산물인 acetaldehyde(100mg/kg)을 복강내로 투여하고 관찰한 실험에서도 간장중 MAO활성이 현저하게 증가되었으며 인삼 butanol분획을 전처리함으로써 정상수준 가깝게 MAO활성이 감소되었다. 이 연구결과로 ethanol에 의한 간장중 MAO활성증가는 ethanol의 대사산물인 acetaldehyde에 기인된 것으로 생각되어지며 인삼 butanol분획이 acetaldehyde에 의해 유도되는 간장중 MAO활성 변동을 조절하고 있을 것으로 사료된다. Effect of ginseng butanol fractions on the hepatic mitochondrial monoamine oxidase activity with ethanol treatment was investigated in this experiment. Ethanol treatment, either acutely or chronically, increased the hepatic mitochondrial monoamine oxidase activity compared to control group. Whereas, treatment with ginseng butanol fractions lowered the ethaol-induced monoamine oxidase activity. Acetaldehyde, the major metabolite of ethanol, significantly increased the hepatic mitochondrial monoamine oxidase activity more than ethanol did. It was also observed that ginseng butanol fractions reduced the increase of the hepatic mitochondial monoamine oxidase activity by acetaldehyde. From these results, it is suggested that ginseng butanol fractions may be associated with the modulation of the hepatic mitochondrial monoamine oxidase activity in ethanol-treated animals.

천마의 항경련작용기전 연구

허근(Keun Huh),이수진(Su Jin Yi),신억섭(Uk Seob Shin),박종민(Jong Min Park) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.3

Gastrodia elata is a medicinal plant which has been used as anticonvulsant in Kurea, Japan and China. This study was conducted to examine the action mechanism of Gastrodia elata centering around the change of GABA and glutamic acid level in brain while observing the anticonvulsive effect in PTZ-induced seizure model. Seizures were reduced effectively by pretreatment of ether soluble part of methanol extract of Gastrodia elata. The pretreatment of ether soluble part inhibited not only the decrease of brain GABA level but also the increase of brain glutamic acid level observed in PTZ model of convulsive dose. Although there was not any change in glutamic acid level, the same development was also observed in the model of subconvulsive dose. From above results, it seems that the anticonvulsive component of Gastrodia elata is lipophilic, and its action mechanism is originated from both control action of GABA level and inhibition of glutaminergic neurotransmission.