It was attempted to observe the effect of garlic on the hepatic xanthine oxidase activity in this study. Xanthine oxidase which catalyze the oxidation of many purine and pyrimidine derivatives was further purified by centrifugation, heating, (NH₄)...
It was attempted to observe the effect of garlic on the hepatic xanthine oxidase activity in this study. Xanthine oxidase which catalyze the oxidation of many purine and pyrimidine derivatives was further purified by centrifugation, heating, (NH₄)₂SO₄fraction, acetone fraction and dialysis, hydroxyapatite column. Allicin and heat-treated allicin fraction powerfully inhibited xanthine oxidase activity in vitro. When the enzyme was incubated in reaction mixture containing garlic butanol fraction, it was found that xanthine oxidase activity was increased. Garlic bulbs and seeds ethanol extracts increased the enzyme activity, whereas leaves and roots extracts did not affected its activity in vitiro.