Acid phosphatase activity in an Acanthamoeba species isolate

Chen, Ko-Hsien,Min, Duk-Young,Kim, Kyung-Min 한양대학교 의과대학 1994 한양의대 학술지 Vol.14 No.2

이 연구에서는 우리나라에서의 담수어에서 분리한 Acanthamoeba species의 생리기능적 구조에 대한 기초적 연구로 세포질내 acid(AcPase)활성능을 관찰하여 다음과 같은 결과를 얻었다. 광학현미경상에서 아메바의 AcPase의 활성을 관찰한 결과 세포내 공포로 여겨지는 부위에서 과립상의 반응을 보였다. 투과전자현미경으로 아메바의 AcPase 활성을 관찰한 결과 대부분의 공포 및 REP에서 AcPase 양성반응을 관찰할 수 있었으며 Golgi-compelx 및 미토콘드리아에서는 AcPase 양성반응을 관찰할 수 없었으나 항혈청으로 처치된 아메바에서는 일부 미토콘드리아에서 Acpase 양성반응을 나타내는 것으로 관찰되었다. 이 때 양성 반응을 보이는 공포들은 (a) 크기가 작으며 구조 전체가 강한 양성 반응을 보이는 lysosome (b) 크기가 크며 한계막 및 내용물이 강한 양성 반응을 보이는 식포들 (c) 전체적으로 한계막과 내용물의 반응이 약한 식포들 (d) 내용물이 있으나 전혀 양성 반응이 없는 식포들 및 (e) 내용물도 없고 음성 반응을 보이는 식포 및 수축포들 등으로 구분할 수 있었다. 또한 강한 Acpase 양성 반응을 보이는 lysosome 또는 vesicle등은 서로 융합하여 커다란 식포를 형성하고 있음이 관찰되었으며 일부, AcPase 양성인 식포는 또 다른 식포 주변에 접해 있거나 융합 또는 연결되어 있음을 관찰하였다. 이상의 결과로 미루어 이 연구에서 관찰된 Acanthamoeaba species는 소화기능에 있어서 lysosome이 서로 융합되거나 다른 식포에 융합, 연결되어 포식된 내용물을 소화하는 것으로 여겨진다.

고콜레스테롤 식이의 급여에 의해 고지혈증이 유도된 흰쥐의 신령버섯 자실체의 항고지혈증 효과

이태수,진가헌 한국버섯학회 2018 한국버섯학회지 Vol.16 No.2

In this study, we aimed to investigate the effects of dietary supplementation with fruiting body of Agaricus brasiliensis (AB) mushroom on the lipid profiles of serum and histological patterns of liver of high cholesterol-fed rats. Five-week-old, female Sprague-Dawley albino rats were divided into three groups of 8 rats each, including a normal control-diet (NC) group, a highcholesterol diet (HC) group, and a group fed high-cholesterol diet supplemented with 5 % powder of Agaricus brasiliensis fruiting bodies (HC+AB). Total serum cholesterol, low density lipoprotein (LDL), and triglyceride (TG) concentrations in the HC+AB group were significantly reduced when compared with those in the HC group. Body weight in the HC+AB group was significantly lower than that in the HC group, whereas no adverse effects were observed on the levels of plasma albumin, creatinine, blood urea nitrogen, uric acid, glucose, and total protein. In the HC+AB group, liver enzyme activities related to liver function, such as GOT and GPT, presented values lower than those in the HC group and were very similar to the ones in the NC group. Excretion of total lipid and cholesterol in feces in the HC+AB group was significantly higher than that in the NC and HC groups, indicating that mushroom feeding inhibits the absorption of lipid cholesterol in the intestine. Liver histopathological analyses revealed that rats fed with HC diet developed fat liver disease, whereas only small amounts of fat were deposited in the livers of the HC+AB group. In conclusion, the results suggest that fruiting body powder of A. brasiliensis provides health benefits to high-cholesterol-fed rats by lowering body weight and the risk of atherogenic lipid profile.

지역사회 주민의 시력장애 이환율 및 영양소 섭취수준

최혜정,진가헌,김영옥 한국식품영양학회 2000 韓國食品營養學會誌 Vol.13 No.5

This study was conducted to estimate the prevalence of ametropias such as myopia and hyperopia from the community based population study. Another purpose of this study was to determine the association between ametropia and nutrient intake. The study subjects was 492 residents over 20 years of age residing in Kuri City. Ametropia was tested based on refrector error using auto referactor. Nutrient intake was estimated from the dietary survey using 24 hour recall method. As a result of data analysis, the prevalence of myopia was 57% and that of hyperopia was 11%. Such a high rate of ametropia prevalence may suggest the increasing risk of eye health in a Korean society. Nutrient intake of myopia group was 5∼15% higher than that of normal group in most of the nutrients. Whereas intake of most nutrient except calcium, retinal, fiber for the hyperopia group was lower than that of normal group. The result may suggest that inbalance of nutrient intake has been related with ametropia.

조짐편두통 환자와 무조짐편두통 환자 간의 평균혈소판용적 차이

반수지,이준범,진가헌,김원용 대한가정의학회 2019 Korean Journal of Family Practice Vol.9 No.4

연구배경: 평균혈소판용적(mean platelet volume)은 혈소판이 활성화될 때 증가하며 편두통 환자에서 평균혈소판용적이 증가되는 것으로 알려져 있다. 본 연구는 조짐편두통 환자와 무조짐편두통 환자의 혈소판 수와 평균혈소판용적을 비교하고자 하였다. 방법: 2012년 1월부터 2017년 6월까지 홍익병원 외래로 내원하여 편두통을 진단받은 환자의 의무기록을 조사하였다. 편두통환자는 조짐편두통 환자와 무조짐편두통환자로 분류하였고, 두 군 간의 혈소판 수와 평균혈소판용적을 비교하였다. 또한 뇌경색, 심혈관 질환 등의 동반 유무도 조사하였다. 결과:조짐편두통환자 46명과무조짐편두통환자 77명이연구에포함되었다. 조짐편두통 환자군이 무조짐편두통 환자군보다 평균혈소판용적이 유의하게 컸고(migraine with aura [MA] 8.92±0.17 fL vs. migraine without aura [MO] 6.32±0.28 fL, P=0.034), 뇌경색 및 심혈관질환의 동반 빈도가 높았다(ischemic stroke, MA 15.2% vs. MO 7.8%,P=0.027; cardiovascular disease, MA 10.9% vs. MO 6.5%, P=0.018). 결론: 평균혈소판용적은 무조짐편두통 환자보다 조짐편두통 환자에서 더 크다. 이는 두 질환 간에 병태 생리적인 차이와 관련이 있을것이다. Background: Mean platelet volume (MPV) increases when platelets are activated, and it is known to increase in migraine patients. The aim of this study is to investigate whether there is a difference in MPV or platelet count between migraine patients with (MA) and without aura (MO). Methods: Migraine patients were recruited from the out-patient department of a hospital between January 2012 and June 2017. Patients were divided into MA and MO groups. Platelet count and MPV were compared between groups, and the frequency of comorbidities such as ischemic stroke and cardiovascular disease, was investigated in both groups. Results: Of the 123 patients, 46 were classified as MA, and 77 were classified as MO. The MPV of the MA group was significantly higher than that of the MO group (8.92±0.17 fL, 6.32±0.28 fL, respectively) (P=0.034). However, platelet count showed no significant difference between groups. Cardiovascular disease and ischemic stroke incidences were significantly higher in the MA group than in the MO group (ischemic stroke: 15.2%, 7.8%, respectively, P=0.027; cardiovascular disease: 10.9%, 6.5%, respectively, P=0.018). Conclusion: Mean platelet volume was significantly greater in the MA group than in the MO group. This may be related to the pathophysiological differences between the two conditions.

동두천지역 고등학생의 안굴절상태 조사 및 연구 II

최혜정,차정원,박문찬,진가헌,Choi, Hae Jung,Cha, Jung Won,Park, Moon Chan,Chen, Ko Hsein 한국안광학회 1998 한국안광학회지 Vol.3 No.1

현재 국내의 고등학생에 대한 굴절이상에 관한 조사는 아주 미흡한 상태이다. 특히 동일집단의 시력을 수년에 걸쳐서 추적 조사한 사례는 거의 전무한 설정이므로 동두천지역 중 고등학생의 3년 전과 현재의 안굴절 상태를 비교 분석하여 근시, 원시, 난시 등 굴절이상의 발생분포 현황을 조사하고, 또한 앞서 보고한 바 있는 일반성인의 시력에 관한 역학조사와 동일한 장비를 이용하여 학생들의 시력을 측정함으로서 성인과 학생의 시력을 비교분석하였다. 시력을 spherical equivalent 기준으로 분류할 때 정시안이 총 대상안의 36.0%, 굴절이상안이 총 대상안의 64.0%(근시 60.6%, 원시 4.4%)를 차지하는 것으로 나타났다. 1995년 학생에 비하여 1998년 학생의 근시안 비율이 증가하였으며, 1998년 학생들이 1997년 일반성인보다 정시안의 비율이 7.4%, 근시안의 비율이 4% 높게 나타났고, 원시안의 비율은 성인에 비하여 매우 낮게 나타났다. 굴절이상안의 종류별 분포는 전체 대상안 1212안 중 단순근시가 전체 대상안 중 28.6%로 가장 많았고, 근시성 복난시가 22.4%로 두 번째로 많았으며, 그 다음은 근시성단난시가 14.4%, 원시성단난시 3.1%, 혼합난시 1.7%, 단순원시 1.2%, 원시성복난시 1.2%의 순서로 나타났다. 난시안의 분포는 Cyl-0.25Dptr를 난시에 포함시켰을 때 전체 대상안의 76.7%를 차지하였으며, -025Dptr를 제외하였을 때는 전체 대상안의 45.6%를 차지하였으며, 학생의 직난시가 도난시의 약 4.5배가량 많은 것으로 나타났다. 우안과 좌안의 굴절력을 비교한 결과 우안이 더 근시 쪽의 이상을 나타내었으며 이는 성인의 경우와 동일한 것으로 조사되었으며, 남녀의 굴절이상에서는 유의한 차이점이 발견되지 않았다. Until now, the study of eye refractive error examination for high school students are a few report that in Korea. In particular, there is few report about the visual power for same group during several years. We compared the study of refractive error done this year with that reported three years ago at the same high school. From the these data, the distribution of ammetropia was investigated. The study of refractive error for high school students was also compared with those reported before for the Adults and the middle school student. When the visual power is refered to spherical equivalent, the 36% of the whole students examined above had emmetropia and the other part of them fumed out to be ammetropia which is classify to 60.6% belonged to myopia and 4.4% belonged to hyperpia. The ratio of myopia for this year students is increased in comparision with the students in 1995. The ratio of emmetropia for this year students is 7.4% higher, and the ratio of myopia for this year students is 4% higher, but the ratio of hyperopia is very lower than that for the adults in 1997. In the kind of refractive error, it is classified that a simple myopia is shown to highest ratio as a 28.6% of 1212 eyes examined, a compound myopic astigmatism to the next high ratio as a 22.4%, a simple myopic astigmatism as 14.4%, a simple hyperopic astigmatism as 3.1%, a mixed astigmatism as a 1.7%, a simple astigmatism as 1.2%, a compound hyperopic astigmatism as 1.2%, respectively. The percentage of an astigmatism is a 76.7% of total eyes examined if Cyl-0.25 Dptr is included to an astigmatism. On the other hand, The percentage of an astigmatism is a 45.6% of total eyes examined if Cyl-0.25 Dptr is excluded to an astigmatism. In the kind of astigmatism, the number of students had an astigmatism with the rule is about 4.5 times than that of astigmatism against the rule. From the result of comparision the right eye with the left eye, the right eye of the students had more a myopic refractive error than the left eye, which is same as adults' case, there is no difference in a refractive error between the boy students and the girl students.

Acute Posterior Multifocal Placoid Pigment Epitheliopathy 1예

권오웅(Oh Woong Kwon),진가헌(Ka Heon Chin),김기창(Ki Chang Kim),김관식(Kwang Shik Kim) 대한안과학회 1985 대한안과학회지 Vol.26 No.5

한국 성인의 굴절이상에 관한 역학조사

최혜정(Hae Jung Choi),진가헌(Ko Hsien Chen),차정원(Jung Won Cha) 한국안광학회 1997 한국안광학회지 Vol.2 No.1

A population-based study of people aged above 20 years showed that 32% had emmetropia and 68% had ammetropia(myopia 56.6%, hyperopia 11.4%) city in Korea. The percentage of ammetropia in population based study is higher than that of clinic(O.P.D.) based. A 83.3% of the ammetropia had myopia, which is higher than 76.3% of 1968 and 76.9% of 1975 years. A 16.7% of the ammetropia had hyperopia, which is lower than 19.4% of 1968 and 17.3% of 1975 years. In the kind of refractive error, 32.1% of 985 eyes examined had compound myopic astigmatism, 18.2% had simple myopic astigmatism, 14.2% had simple myopia, 6.8% had simple hyperopic astigmatism, 5.0% had mixed astigmatism, 4.7% had compound hyperopic astigmatism and 3.6% had simple hyperopia. In the difference of binocular refractive error, 29~? had 0.50∼2.00 Dptr difference and 3.6% had difference above 2.00 Dptr. In age related myopic refractive error, 76.7% of people aged 20∼29 years and 74.0% of 30∼39 years had myopia. It is due to overstudy for entrance into a university that the percentage of myopia is higher than that of abroad. In age related hyperopic refractive error, 2.9% of people aged 20∼29 years, 0.6% of 3039 years, 6.3% of 40∼49 years, 16.0% of 50-59 years and 63.9% of 60∼69 years had hyperopia. It shows that the age related hyperopic refractive error was significantly increased at aged 40∼49 years. The right eye had more myopic refractive error than left eye.

동두천지역 고등학생의 안굴절상태 조사 및 연구2

박문찬(Moon Chan Park),진가헌(Ko Hsein Chen),최혜정(Hae Jung Choi),차정원(Jung Won Cha) 한국안광학회 1998 한국안광학회지 Vol.3 No.1

Until now, the study of eye refractive error examination for high school students are a few report that in Korea. In particular, there is few report about the visual power for same group during several years. We compared the study of refractive error done this year with that reported three years ago at the same high school. From the these data, the distribution of ammetropia was investigated. The study of refractive error for high school students was also compared with those reported before for the Adults and the middle school student. When the visual power is refered to spherical equivalent, the 36 % of the whole students examined above had emmetropia and the other part of them fumed out to be ammetropia which is classify to 60.6 % belonged to myopia and 4.4 % belonged to hyperpia. The ratio of myopia for this year students is increased in comparition with the students in 1995. The ratio of emmetropia for this year students is 7.4 % higher, and the ratio of myopia for this year students is 4 % higher, but the ratio of hyperopia is very lower than that for the adults in 1997. In the kind of refractive error, it is classified that a simple myopia is shown to highest ratio as a 28.6 % of 1212 eyes examined, a compound myopic astigmatism to the next high rartio as a 22.4 %, a simple myopic astigmatism as 14.4 %, a simple hyperopic astigmatism as 3.1 %, a mixed astigmatism as a 1.7 %, a simple astigmatism as 1.2%, a compound hyperopic astigmatism as 1.2%, respectively. The percentage of an astigmatism is a 76.7 % of total eyes examined if Cyl-0.25 Dptr is included to an astigmatism. On the other hand, The percentage of an astigmatism is a 45.6 % of total eyes examined if Cyl-0.25 Dptr is excluded to an astigmatism. In the kind of astigmatism, the number of students had an astigmatism with the rule is about 4.5 times than that of astigmatism against the rule. From the result of comparition the right eye with the left eye, the right eye of the students had more a myopic refractive error than the left eye, which is same as adults` case, there is no difference in a refractive error between the boy students and the girl students

각막굴절력과 시력의 관련성에 관한 고찰

차정원(Jung Won Cha),진가헌(Ko Hsein Chen) 한국안광학회 1999 한국안광학회지 Vol.4 No.1

학술발표 3 : UV meter용 패브리-페로 간섭필터 제작 및 광학특성연구

박문찬,정부영,최혜정,진가헌,황보창권,김형두 한국안광학회 2004 2004년 국제학술심포지엄 Vol.- No.-