16S rDNA-RFLP에 의한 Spirastrella abata와 Spirastrella panis 해면에 서식하는 배양가능한 공생세균 군집의 비교

조현희,박진숙,Cho, Hyun-Hee,Park, Jin-Sook 한국미생물학회 2009 미생물학회지 Vol.45 No.2

계통적으로 근연하며 지리적 분포가 유사한 두 종의 Spirastrella 속의 해양 해면, S. panis와 S. abata의 배양 가능한 공생세균 군집구조를 16S rDNA-RFLP 방법에 의해 분석하였다. 공생세균의 배양은 해면추출물 3%를 포함하는 MA 배지를 사용하였다. 증폭된 16S rDNA의 RFLP (restriction fragment length polymorphism) 분석을 위한 제한효소로 HaeIII와 MspI을 이용하였으며, 그 결과 24개의 RFLP type을 구별할 수 있었다. 각 패턴별로 1~5개의 분리균주를 선별하여 부분 염기서열 분석 결과, 알려진 세균 종과 98.4% 이상의 유사도를 나타내었으며 2종의 Spirastrella 해면으로부터 분리된 세균들은 모두 Alphaproteobacteria, Gammaproteobacteria, Firmicutes, Actinobacteria 4개의 강(class)에 포함되었다. Alphaproteobacteria는 S. abata에서 39.3%, S. panis에서 47.6%가 관찰되어 두 해면에서 우점하는 세균 군집이었다. Gammaproteobacteria의 경우 S. abata에서 38.5%로 관찰된 반면 S. panis에서 1.6%의 아주 적은 비율로 관찰되었다. 또한 Bacillus (phylum Firmicutes) 종은 S. abata에서 9.7%를 나타낸 반면, S. panis에서는 44.3%의 분포를 나타내었다. Planococcus maritimus (8.1%, phylum Firmicutes)와 Psychrobacter nivimaris (28.9%, phylum Gammaproteobacteria)는 S. abata에서만 관찰되어 이들은 S. abata에 특이적인 세균 종임을 알 수 있었다. 같은 장소에 서식하는 계통적으로 근연한 두 종의 해면에서 공생세균의 군집 구조는 차이가 큰 것으로 나타났다. A cultivation-based approach was employed to compare the culturable bacterial diversity associated with two phylogenetically closely related marine sponges, Spirastrella abata and Spirastrella panis, which have geologically overlapping distribution patterns. The bacteria associated with sponge were cultivated using MA medium supplemented with 3% sponge extracts. Community structures of the culturable bacteria of the two sponge species were analyzed with PCR-RFLP (restriction fragment length polymorphism) based on 16S rDNA sequences. The RFLP fingerprinting of 16S rDNA digested with HaeIII and MspI, revealed 24 independent RFLP types, in which 1-5 representative strains from each type were partially sequenced. The sequence analysis showed >98.4% similarity to known bacterial species in public databases. Overall, the microbial populations of two sponges investigated were found to be the members of the classes; Alphaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria. The Alphaproteobacteria were predominant in the bacterial communities of the two sponges. Gammaproteobacteria represented 38.5% of bacterial community in S. abata. Whereas only 1.6% of this class was present in S. panis. Bacillus species were dominat in S. panis. Bacillus species were found to be 44.3% of bacterial species in S. panis, while they were only 9.7% in S. abata. It is interesting to note that Planococcus maritimus (8.1%, phylum Firmicutes) and Psychrobacter nivimaris (28.9%, phylum Gammaproteobacteria) were found only in S. abata. This result revealed that profiles of bacterial communities from the sponges with a close phylogenetic relationship were highly species-specific.

해면 Spirastrella abata와 Cinachyrella sp.의 공생 세균의 계통학적 다양성

조현희,심은정,박진숙,Cho, Hyun-Hee,Shim, Eun-Jung,Park, Jin-Sook 한국미생물학회 2010 미생물학회지 Vol.46 No.2

2009년 4월 제주도의 운진항에서 채집한 해양 해면 Spirastrella abata와 Cinachyrella sp.의 공생 세균의 주요 군집구조를 16SrDNA-denaturing gradient gel electrophoresis (DGGE) 방법을 이용하여 비교 분석하였다. S. abata와 Cinachyrella sp.는 각각 7개와 8개의 DGGE 밴드를 나타내었으며 이들을 적출하여 염기서열을 분석한 결과, NCBI에 등록된 서열들과 92-100%의 유사도를 나타내었다. S. abata의 주요 공생세균은 Alphaproteobacteria와 Deltaproteobacteria에 속하였으며, Cinachyrella sp.의 경우 Alphaproteobacteria, Gammaproteobacteria와 Actinobacteria에 속하는 세균으로 구성되었다. 두 종의 해면에서 공통되는 공생세균 그룹은 Alphaproteobacteria였으며 이 세균 그룹은 두 종의 해면 모두에서 우점하였다. Deltaproteobacteria는 S. abata에서, Actinobacteria와 Gammaproteobacteria는 Cinachyrella sp.에서만 관찰되었다. 동일지역에서 채집한 서로 다른 두 종의 해면은 각각 다른 공생세균 군집구조를 나타내었다. The bacterial community structure of two marine sponges, Spirastrella abata and Cinachyrella sp. collected from Jeju Island, in April 2009, was analyzed by 16S rDNA-denaturing gradient gel electrophoresis (DGGE). DGGE banding patterns indicated 8 and 7 bands for Spirastrella abata and Cinachyrella sp., respectively. Comparative sequence analysis of variable DGGE bands revealed from 92% to 100% similarity to the known published sequences. The bacterial groups associated with Spirastrella abata were Alphaproteobacteria and Deltaproteobacteria. The bacterial community of Cinachyrella sp. consisted of Alphaproteobacteria, Gammaproteobacteria, and Actinobacteria. Alphaproteobacteria was common and predominant in both the sponge species. Deltaproteobacteria was found only in Spirastrella abata while Actinobacteria and Gammaproteobacteria were found only in Cinachyrella sp. The results revealed that though the common bacterial group was found in both the sponges, the bacterial community profiles differed between the two sponge species obtained from the same geographical location.

보비탕(補脾湯)이 methotrexate로 유발(誘發)된 흰쥐의 면역기능저하(免疫機能低下)에 미치는 영향(影響)

조현희,윤혜진,서정민,백정한,Cho, Hyun-Hee,Yun, Hye-Jin,Seo, Jung-Min,Baek, Jung-Han 대한한방소아과학회 2007 대한한방소아과학회지 Vol.21 No.1

Objectives : In order to investigate the effect of Bobitang on SD rats with deteriorated immunity caused by methotrexate. Methods : The test sample were dosed once a day for 14 days by gastric gavage at a dosage 1,000, 500 and $250mg/kg/10m{\ell}$ from 2 days after last MTX-dosing, and the changes on body weight and gains, spleen weight and total blood leukocyte numbers, total lymphocyte numbers, the percentage of B-cell, T-cell, CD3+CD4+ T-cell, CD3+CD8+ T-cell and CD4+/CD8+ T-cell ratios in the blood and spleen were observed. Results : The changes on body weight gains, the spleen weight, the total blood leukocyte numbers, the total lymphocyte numbers in the blood and spleen, the ratio of T-cell in the blood and spleen, the ratio of CD3+CD4+ T-cell in the blood and spleen were increased significantly in BBT Extracts groups as compared with control group. The ratio of B-cell in the blood and spleen was not increased significantly in BBT Extracts groups as compared with control group. The percentage of CD3+CD8+ T-cell in the blood and spleen was decreased significantly in BBT Extracts groups as compared with control group. The ratio of CD4+/CD8+ T-cell in blood and spleen was increased significantly in BBT Extracts group as compared with control group. Conclusions : According to the above results, Bobi-Tang has an effect of increasing immune responses on SD rats with deteriorated immunity caused by methotrexate.

고정관념과 편견의 근원을 찾아서

조현희(Cho, Hyunhee) 학습자중심교과교육학회 2017 학습자중심교과교육연구 Vol.17 No.18

본 연구는 자신이 가진 고정관념과 편견의 근원에 대한 예비교사들의 인식과 경험을 통해 문화적비판의식의 발달 양상 및 관련 요인들을 탐구하는 데 목적이 있다. 수도권 지역 K대학에 개설된 다문화교육 강의에 참여한 예비교사 31명의 자기성찰 보고서를 내러티브 탐구의 절차와 방법에 따라 수집, 분석한 결과는 다음과 같다. 첫째, 예비교사들이 인식한 고정관념과 편견의 근원은 (1) 타인과의 직접적인 만남과 경험, (2) 가정, 학교, 지역사회 등 자신이 속한 집단이나 미디어를 통한 학습, (3) 고정관념과 편견을 생산, 유통한 주류집단 등 개인적, 사회적인 수준에 걸쳐 다양하게 나타났으며, 이러한 근원에 대한 예비교사들의 인식을 유형화함으로써 문화적비판 의식 발달 양상이 도출되었다. 둘째, 가정, 학교, 미디어 등을 통해 기존의 고정관념과 편견에 도전하는 정보나 사례를 접한 경험, 역위치성(소수화)의 경험, 자기성찰 경험 등은 고정관념과 편견의 근원에 대한 예비교사들의 인식을 확장시키며 이들의 문화적비판의식 발달을 촉진하였다. 연구 결과들을 토대로 향후 다문화교사교육을 위한 시사점을 제안하였다. This study sheds light on South Korean prospective teachers’ cultural critical consciousness by exploring how they perceive origins of their stereotypes and biases and how these conception are related to their prior experiences. Guided by narrative inquiry, I analyzed data collected from 31 prospective teachers’ self-reflection notes. The findings highlight that the prospective teachers attributed their stereotypes and biases to (1) their face-to-face experiences with others, (2) the sites or paths of socialization such as home, school, community, and mass media, and/or (3) the agents of socialization; and by combining each of the different attributions three patterns of cultural critical consciousness were emerged. The findings also revealed three interconnected experiences that had a significant impact on deepening the prospective teachers’ cultural critical consciousness: facing counter-cases within/through home, school, and/or mass media; experiencing reversed power dynamics (being marginalized); and being involved in self-reflections. Recommendations for teacher education were also provided.

폐경 여성에서 호르몬 요법이 인체 내 미네랄 농도와 삶의 질에 미치는 영향

조현희 ( Hyun Hee Cho ),정재은 ( Jae En Jung ),정인철 ( In Cheul Jung ),김민정 ( Min Jung Kim ),김수연 ( Sue Youn Kim ),황성진 ( Sung Jin Hwang ),김미란 ( Mee Ran Kim ),김장흡 ( Jang Heub Kim ),김진홍 ( Jin Hong Kim ) 대한폐경학회 2007 대한폐경학회지 Vol.13 No.3

연구목적: 인체 조직내의 미네랄은 인체의 스트레스 반응과 면역체계, 에너지 생산을 조절하며, 혈액 내에서 보다는 조직에서 쉽게 불균형을 나타내어 질병의 조기지표 (early indicator) 로서의 의미를 갖는다. 폐경 후 증가하는 미네랄의 불균형 상태는 환자의 질병 전 상태를 예측하고 삶의 질을 저하시키는 원인으로 작용한다. 연구재료 및 방법: 본 연구에서는 정기검진을 위해 병원을 방문한 45∼60세의 폐경 여성을 호르몬 대체요법을 받은 군과 받지 않은 군, 나이가 45∼55세인 군과 55∼60세인 군, 호르몬 대체요법을 3년 미만으로 받은 군과 3년 이상 받은 군으로 나누어 군별로 SF-36 설문지의 점수가 차이가 있는 지를 조사하였다. 또한 이들에게서 조직 미네랄 검사와 혈액검사, SF-36 설문지를 작성하게 하였으며 설문지의 점수와 모발 미네랄 검사상의 미네랄 수치 (Ca, Mg, Na, K, Cu, Zn)가 어떠한 관계를 갖는지 확인하였다. 결과: 환자의 나이가 45∼55세인 군은 55∼60세 인 군에 비해 신체적 기능 (physical function), 신체활동점수 (summary measures of physical)와 정신건강점수 (summary measures of mental health)에서 더 좋은 점수를 나타냈다 (P<0.01). 호르몬 치료를 하는 사람과 하지 않는 사람의 비교에서는 호르몬 치료를 받는 군에서 통증 (Bodily pain), 일반건강 (General health),신체적 기능 (Physical function), 신체적 역할제한 (Role limitation - physical), 감정적 역할제한 (Role limitation - emotion), 신체활동점수 (summary measures of physical)에서 더 좋은 점수를 나타냈다 (P<0.01). 조직내 미네랄 함량을 기준으로 비교한 결과, 조직 마그네슘과 아연이 정상인 군이 비정상군에 비해 일반건강 (General health) 점수가 더 우수한 것으로 나타났다 (P<0.01). 결론: 폐경 여성에서 호르몬 요법의 유무에 따라 환자가 느끼는 건강수준에 차이가 있는 것으로 나타났으며, 조직 내 아연과 마그네슘의 수치가 일반건강 수준에 영향을 주는 것으로 나타났다. Objectives: Minerals in human tissue are involved in regulation of stress reaction, immune reactivity and energy production. They also serve as an important early indicator of disease because the imbalance in tissue minerals usually proceeds the mineral change in blood. In postmenopausal women, increased mineral imbalance helps to predict the subclinical diseased state and provides a factor to decrease quality of life. Often postmenopausal women complain decreased general health condition and decreased sense of well-being without noticeable diseases. There are controversial published reports that it might be related to postmenopausal hormonal changes. Methods: In this study, postmenopausal women aged between 45 and 60 who visited our hospital for routine check up were enrolled. Tissue mineral assay and blood mineral assay were performed and SF-35 questionnaires were completed. Relationship between questionnaire scores and tissue mineral levels from patient`s hair (Ca, Mg, Na, K, Cu, Zn) was evaluated. They were compared between hormone therapy user and nonuser groups, aged 45∼55 and aged 55∼60 groups, and receiver of hormone therapy for more than 3 years and receiver for hormone therapy started more than 3 years after menopause groups. Results: Younger group (45∼55 years) showed better status than aged group (55∼60 years) in Physical function, Summary measures of physical health, and Summary measures of mental health (P<0.01). Hormone therapy user group showed better status than nonuser group in Bodily pain, General health, Physical function, Role limitation - physical, Role limitation - emotion and Summary measures of physical (P<0.01). Groups with normal tissue mineral levels within reference range of zinc and magnesium levels showed better scores than out of range group in General health (P<0.01). Conclusion: For conclusion, postmenopausal hormone therapy increases well-being sense after menopause, and abnormal levels of tissue zinc and magnesium affect general health status.

초등 예비교사의 영어 언어유희 텍스트에 대한 반응과 인식

조현희(Cho, Hyunhee) 언어과학회 2021 언어과학연구 Vol.- No.99

This study inquired about primary pre-service teachers" responses to reading children"s English language play texts and explored the implications for teaching English in primary classrooms. Seven primary pre-service teachers attending an educational university participated in the study. A group interview and an individual interview with each of them comprised the data source. The analysis of the interview data revealed that first, the participants" recognition of the playful features of language was secondary to their comprehension of the literal meaning of words and sentences. Second, the forms of language play that the participants were most familiar to was rhyme and rhythm. Third, the participants" responses to language play texts were found to be somewhat related to their English learning experiences. The findings of the study has implications for English education and teacher education.

꼬리뼈하 천골고정술: 자궁탈출증 치료의 단기성적보고

조현희 ( Hyun Hee Cho ),김미란 ( Mee Ran Kim ),황성진 ( Sung Jin Hwang ),김민정 ( Min Jung Kim ),김은중 ( En Jung Kim ),이진우 ( Jin Woo Lee ),김장흡 ( Jang Heub Kim ),김진홍 ( Jin Hong Kim ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.6

목적: 자궁탈출증 및 질말단부탈출증으로 꼬리뼈하 천골고정술을 시행받은 34명의 여성의 6개월 단기추적결과를 보고하고자 한다. 연구방법: 자궁탈출증 및 질말단부탈출증이 있는 여성 34명에서 꼬리뼈하 천골고정술을 시행하여 수술 중/ 수술 후 나타날 수 있는 합병증과 수술 6개월 뒤 재발 여부에 대해 추적검사를 실시하였다. 결과: 꼬리뼈하 천골고정술 후 혈색소의 변화는 1.7±0.9로 나타났으며, 도뇨관 유치기간은 3.1±1.0로 수술 후 평균 2.1일에 도뇨관을 제거하고 자가배뇨가 가능하였다. 입원기간은 5.2±1.0로 수술 후 평균 3.2일이면 퇴원이 가능하였다. 6개월 재발율은 2.9%였다. 수술 전 환자들의 자궁/질말단부 탈출증의 정도는 평균 2.7±0.7 (stage)였으며, 수술 후 6개월에 확인한 탈출증의 정도는 0.4±0.6 (stage)이었다. 결론: 꼬리뼈하 천골고정술은 자궁적출술 없이 효과적으로 자궁탈출증을 치료할 수 있는 방법이다. Objective: To investigate the short term results of infracoccygeal sacropexy for the management of uterine prolpase or stump prolapse. Methods: Thirty-four Women who underwent infracoccygeal sacropexy for the management of uterine prolapse or stump prolpase were enrolled this study. Intraoperative or postoperative complications and results of the operation were evaluated and the recurrence rate of pelvic organ prolapse was checked 6 months after operation. Results: Changes of hemoglobin after infracoccygeal sacropexy was 1.7±0.9, mean duration of bladder drainage was 3.1±1.0 (days). After 2.1 days of operation, foley catheter was removed. Mean inpatient day was 5.2±1.0, patient discharged 3.2 days after operation. Recurrence rate after 6 months was 2.9%. Preoperative mean stage of prolapse was 2.7±0.7 (stage) and it decreased to 0.4±0.6 (stage) 6 months after operation. Conclusions: Infracoccygeal sacropexy was effective method for the management of uterine/ stump prolapse.

예비 초등영어교사들의 영어학습 전략체계의 구성과 사용

조현희(Cho, Hyunhee) 한국초등영어교육학회 2011 초등영어교육 Vol.17 No.1

This study aimed at discovering how preservice primary English teachers systematically constructed and used metacognitive knowledge, metacognitive strategies, and cognitive strategies and what contexts came into play in their use of strategy systems. Four teachers college students in an English education program participated in this study. Their English-learning journals and a group interview comprised the data of the study. Analysis of the data revealed that the participants showed both differences and commonalities in the way they formed and used their strategy systems. In a highly organized learner, metacognitive knowledge, metacognitive strategies, and cognitive strategies were found to be linked together and used coherently, consistently, and reflectively; in the less organized learners, the three components were found to be used less so. It was also found that examining the social contexts surrounding the learners could give a better understanding of their construction and use of strategy systems. This study has a few implications for teaching and future research into strategies.

과학문화에 대한 확장적 개념화와 해외 바우처 사례분석을 통한 과학문화 바우처 활성화 방안 탐색: 다학제적 접근

조현희(Cho, Hyun hee),우라미(Woo, Ra mi),장신호(Jang, Shin ho),김근희(Kim, Keun hee),나훈희(Na, Hun hee) 한국전시산업융합연구원 2021 한국과학예술융합학회 Vol.39 No.2

본 연구는 과학문화를 활성화하기 위한 기제로서 바우처가 지닌 가능성에 착안하여 시작되었다. 본 연구의 목적은 과학문화 바우처에 대한 이론적 논의와 해외 바우처 사업 운영사례에 대한 분석을 바탕으로 바우처를 활용한 과학문화 활성화 방안을 모색하는데 있다. 이를 위해 본 연구에서는 과학문화의 개념 및 바우처 사업 운영에 관한 문헌자료와 선행연구를 분석함으로써 과학문화를 이해하기 위한 개념적 틀을 도출하고, 해외 주요국의 성공적인 바우처 운영 사례를 살펴보았다. 연구 결과는 다음과 같다. 첫째, 바우처를 통한 과학문화의 양적 확대와 질적 성장을 도모하기 위해 기존 바우처 사업에 적용되어 온 유사시장론(quasi-market)을 넘어 서비스산업화와 신(新)산업 육성에 중점을 둔 사업 운영이 필요한 것을 알 수 있었다. 둘째, 해외 주요국(미국, 프랑스, 네덜란드)의 사례를 분석한 결과, 과학문화 바우처 사업 운영에 있어 통합성을 강화한 인프라 구축, 과학문화의 양적, 질적성장을 동시에 고려한 관계(network) 및 기술(technical) 차원의 지원, 자원봉사를 통한 범시민적 참여확대 등이 중요함을 알 수 있었다. 이러한 연구 결과를 바탕으로 과학문화의 양적, 질적 성장과 과학문화 산업의 육성을 도모하기 위한 과학문화 바우처 사업의 방향과 과제들을 논의하였다. This research is based on the potential of vouchers as a mechanism to stimulate the science culture of South Korea. The purpose of this study is to explore strategic plans to stimulate the science culture utilizing vouchers, based on theoretical discussion and overseas cases of culture vouchers. The concepts of science culture and administrative plans for vouchers are analyzed through the previous studies and literature. This research shows that existing voucher plans need to move beyond quasi-market framework toward advanced voucher plans focused on industrializing services and fostering new industries in order to achieve expansion and internal reinforcement of science culture. Based on the overseas cases of the United States, France, and the Netherlands, this research presents the importance of building integrated-infra networks, and technical and relational support policy for achieving expansion. The authors also address the necessity to focus on internal reinforcement of science culture, and encouraging citizens to participate in the science voucher programs. Based on the results, we expect to invigorate the science culture voucher policy and eventually expand prospective space for nurturing science culture industries throughout the nation.

초등영어 교사의 교수,학습에 대한 신념의 양상과 역할

조현희 ( Hyun Hee Cho ),김형준 ( Hyung Jun Kim ) 한국현대영어영문학회 2010 현대영어영문학 Vol.54 No.1

This study explored four elementary school teachers` beliefs about teaching English and discovered how their beliefs influenced their teaching practice. An in-depth individual interview was conducted for the study. The study demonstrates that teachers had some beliefs in common, while other beliefs differed between one another. Their common beliefs were shaped by the collective experience of teaching English in the elementary school. The beliefs that differed between individual teachers were formed by their personal teaching and learning experiences as well as local contexts of the classroom. It was discovered that the teachers made efforts to put their beliefs into practice using whatever resources that were available to them. They could not implement what they believed, however, when it involved global social context. This study suggests that teachers` voice should be heard in order to improve elementary English education, and that more attention should be paid to teachers` beliefs in teacher education programs. (Daegu National University of Education, Daegu Cheong-rim Elementary School)