고강도 인터벌 트레이닝이 D-Gal/LPS로 유도된 마우스의 급성간 부전에 미치는 효과

조진경 ( Jin-kyung Cho ),박수현 ( Soo-hyun Park ),강현식 ( Hyun-sik Kang ) 한국운동생리학회(구 한국운동과학회) 2017 운동과학 Vol.26 No.3

PURPOSE: This study investigated the protective role of high-intensity interval training against acute liver injury induced by D-galactos-amine (D-Gal)/lipopolysaccharide (LPS). METHODS: A total of 30 male BALB/c mice aged 5-week were randomly assigned to high-intensity, interval training group (EX, n=10) or control group in cage (Non-EX, n=20) for 10 weeks. Peritoneal injection of D-Gal (700 mg/kg body weight) and LPS (10 μg/kg body weight) was applied to induce acute liver injury, and liver tissue was harvested 6 hours after the injection. Hematoxylin and Eosin (H&E) staining was used for liver histology. Real-time PCR was used to quantify expression of pro-inflammatory and anti-inflammatory genes in the liver. RESULTS: The liver histology showed that D-Gal/LPS treatment resulted in hepatic damage and increased number of neutrophils in conjunction with upregulation of hepatic IL-6 and TNF-α mRNAs and downregulation of hepatic PPARα and SIRT1 mRNAs. On the other hand, the 10-week interval training resulted in a significant improvement in cardiorespiratory fitness assessed as run time to exhaustion on a treadmill. In addition, the interval training attenuated the D-Gal/LPS-induced liver damage and increased number of neutrophil in conjunction with downregulation of hepatic IL-6 and TNF-α mRNAs and upregulation of hepatic PPARα and SIRT1 mRNAs. CONCLUSIONS: This study suggests that high-intensity interval training suppresses the D-Gal and LPS-induced acute liver damage and inflammatory responses.

운동이 중년여성 말초단핵구세포의 reverse cholesterol transport pathway 유전자 발현에 미치는 영향

조진경 ( Jin Kyung Cho ),김영민 ( Young Min Kim ),진영윤 ( Young Yoon Jin ),하창덕 ( Chang Duk Ha ),최규환 ( Kyu Hwan Choi ),홍혜련 ( Hye Ryun Hong ),이신호 ( Shin Ho Lee ),강현식 ( Hyun Sik Kang ) 한국운동생리학회(구-한국운동과학회) 2012 운동과학 Vol.21 No.1

본 연구의 목적은 건강한 중년 여성(n=B)을 대상으로 급성운동과 지속적 인 운동 트레이닝을 통하여 운동이 혈중 지질 개선과 RCT pathway에 미치는 효과를 검정하는 것으로 하였다. 급성운동은 모든 피험자가 운동처치와 통제기간을 모두 수행하도록 single-group cross-over design을 이용하여 최대산소섭취량의 65% 강도에서 l회 400 kcal, 총 3일간 1,200 kcal 운동 에너지 소모하도록 한 후 3일간의 통제기간을 가졌다. 급성운동과 4주 운동 트레이닝의 모든 측정은 공복상태에서 전체 피험자를 대상으로 신체구성측정(체중, 체질량지수, 체지방, 허리둘레), 혈액채취(중성지방, 총 콜레스테롤. 고밀도지단백콜레스테롤, apolipoprotein A-1. RCT pathway 관련 mRNA 정량화(ABCAl mRNA. ABCGl mRNA. SR-Bl mRNA) 등을 실시하였다. 반복이 있는 일원변량분석과 대응표본 t-test를 이용하여 자료 분석을 실시한 결과 급성 운동은 혈중 중성지방을 유의하게 감소시키는 효과를 유도했지만 다른 혈액 변인이나 RCT pathway 유전자 발현에는 유의한 영향을 미치지 못한 것으로 나타났다. 반면 4주 운동트레이닝은 혈중 고밀도지단백콜레스테롤과 apoA-l을 유의하게 증가시킬 뿐만 아니라 RCT pathway 유전자 중에서 ABCGl mRNA 유전자 발현이 운동 트레이닝에 의해 유의하게 증가했으며 ABGAl mRNA 유전자 발현도 증가하는 경향이 있는 것으로 나타났다. 이러한 연구 결과를 종합하여 판단할 때, 급성운동과는 달리 지속적인 운동 트레이닝은 RCT pathway 유전자 발현과 혈중 고밀도지단백콜레스테롤 및 apoA-l에 긍정적인 효과를 미치는 것으로 확인되었다. The pllrJXl5e of the present study was to investigate the effects of both acute exercise and exercise training on blood lipids and mRNAs expression of RCT pathway in middle-aged women (n=8). In a single-group cross-over design, subjects performed a 3-day consecutive exercise at an intensity of 65% V02max targeting at energy expenditure of 1.200 kcal (400 kcal/day) and a 3-day control period. And they were further asked to participate in a 4-week exercise training at the same intensity of 65% VQ2max with a frequency of 3 days per week. The measured outcomes of the study inducted body composition (i.e., weight, body mass index. body fat. waist circumference), blood lipoprotein lipids (i.e., triglycerides. total cholesterol, high-density lipoprotein cholesterol, apolipoprotein A-1) and RCT pathway mRNAs (i.e., ABCAI mRNA. ABCGl mRNA, SR-Bl mRNA) in peripheral blood mononuclear cells (PBMC). One-way RM ANOVA followed by LSD post-hoc and paired Hest were utilized to test any significant changes in the outcomes of the acute exercise and exercise training, respectively. The findings of the study showed that 1) acute exercise induced a significant decrease in serum triglycerides, with no other significant changes in any of the measured variables and 2) unlike acute exercise, exercise training resulted in significant increases in high-density lipoprotein cholesterol and apolipoprotein A-l as well as a significant increase in PBMC ABCGl mRNA. In summary, the current findings of the study suggest that aerobic exercise training can contribute to improved RCT pathway functioning in middle-aged women as shown in increases in HDLC, apoA-l, and ABCGl mRNA.

3T3-L1 지방전구세포에서 합토글로빈에 의한 염증성 cytokine 발현 조절

조진경(Jin-Kyung Cho),김남훈(Nam-Hoon Kim),오미경(Mi-Kyung Oh),박선주(Seon-Joo Park),김인숙(In-Sook Kim) 한국생명과학회 2008 생명과학회지 Vol.18 No.4

백색지방조직(white adipose tissue)은 에너지 저장뿐만 아니라 다양한 adipokines을 분비하는 중요한 내분비 기관이다. 급성기반응 단백질로 알려져 있는 합토글로빈(haptoglobin)도 adipokine의 한 종류로서 지방세포에서 합성되고 분비된다. 그러나 adipokine으로서의 기능과 지방조직에서의 역할은 아직까지 규명되지 않았다. 본 연구에서는 3T3-L1 지방전구세포를 합토글로빈 유전자로 transfection시켜 합토글로빈을 과발현하는 세포를 만들고 세포증식, 염증관련 인자들의 발현조절 및 단구세포의 유인성을 조사하였다. 그 결과, 합토글로빈은 3T3-L1 세포의 성장에는 별 영향을 미치지 않았으나 IL-6와 COX-2 발현을 저해하고 HO-1 합성을 증가하였다. 또한 THP-1 단구세포를 이용한 invasion assay에서는 합토글로빈이 단구세포의 이동을 저해하였다. 이러한 결과들은 합토글로빈이 지방조직에서 항염증 반응에 관여함을 시사한다. 만성적 염증상태(chronic low-grade inflammatory state)로 인식되고 있는 비만은 염증관련 인자들에 의한 인슐린저항성이 유도되는 바, 합토글로빈은 비만 관련 인슐린저항성을 방지하고 인슐린민감성을 향상시키는 데에도 기여할 것으로 생각된다. White adipose tissue is now recognized as an important endocrine organ which secretes various signal factors and proteins termed ‘adipokine’. Haptoglobin (Hp), which has been known as an acute phase protein, belongs to the adipokine. However, the function of Hp in adipose tissue remains unclear. To verify the role of Hp in preadipocytes, in this study, 3T3-L1 preadipocyte cells were stably transfected with human Hp gene and Hp-overexpressing cells were made. The Hp had no effect on cell growth of preadipocytes. By RT-PCR and Western blot analysis, the Hp inhibited gene expression of IL-6 and COX-2 and enhanced HO-1 synthesis in preadipocytes. Moreover, invasion assay showed the Hp suppressed migration of monocytes to preadipocytes. These findings suggest that the Hp may inhibit an inflammatory reaction in adipose tissue by regulating the expressions of pro-inflammatory and anti-inflammatory mediators, and by repressing monocytes/macrophages infiltration.

종설 : 노인의 근감소성 비만 예방 및 치료를 위한 단백질 섭취 전략

조진경 ( Jin Kyung Cho ),강현식 ( Hyun Sik Kang ),윤진환 ( Jin Hwan Yoon ) 대한비만학회 2013 The Korean journal of obesity Vol.22 No.2

인구의 고령화는 수명 연장, 출산율 저하, ‘베이비 붐’ 세대의 고령화로 인해 전 세계적으로 급격한 증가 추세에 있다. 노화는 신체구성에 다양한 변화를 일으키며 이로 인한 질병 중근감소성 비만이 대표적인 예이다. 노화로 인한 근감소성 비만은 좌식생활과 불충분한 단백질 섭취와 같은 생활 습관 요인과 관련 있다. 선행연구에 의하면 근감소성 비만은 생활 습관 중재로 예방 및 치료가 가능한 것으로 나타났다. 따라서 노인들은 1일 단백질 섭취 권장량(0.8~1.6 g/kg)을 충족하는 것이 근육량과 근력 손실을 최소화 하는데 중요한 영양 섭취 전략이 될 것이다. 따라서 노화에 따른 근감 소성 비만의 발생을 지연시키기 위해 저항성 운동과 더불어 충분한 단백질 섭취가 병행 되어 져야 한다. Population aging is occurring worldwide due to increased longevity, declining fertility, and the aging of “baby boom” generations. The aging process involves numerous changes in body composition that affect health among which sarcopenic obesity is of clinical and functional significance. Aging-related sarcopenic obesity is closely related to lifestyle factors, including physical inactivity and inadequate dietary intake of proteins. Considerable evidence suggests that sarcopenia is a reversible cause of disability and could benefit from a lifestyle intervention. Thus, increasing protein intake (ranging 0.8 g/kg/day to 1.6 g/kg/day) in elderly population, especially among frail elderly population, is currently recommended to minimize declines in muscle mass and strength. Further, increased dietary intake of proteins combined with resistance exercise is widely accepted as the most effective intervention to slow the sarcopenic process of aging.

요가수련 참가여부에 따른 청소년의 신체적 자기개념이 심리적 안녕감에 미치는 영향

조진경(Cho Jin-Kyung),하상원(Ha Sang-Won),김종진(Kim Jong-Jin) 한국체육과학회 2009 한국체육과학회지 Vol.18 No.4

The purpose of this study was to investigate the influence of physical self-description on psychological well-being according to yoga participants of adolescents. The data collected from the 515 middle school and high school students by convenience sampling method was analyzed. With the collected data, t-test and multiple regression analysis by SPSSWIN 12.0. The results of the analysis were summarized as follows. First, regarding the physical self conceptualization by adolescents, in the factors of appearance, flexibility, sense of sports capability, muscular strength, self-respect, health, as well as overall physical well-being, the results for students participating in the yoga training were higher than those for non-participating students, while there were no observed differences in the factors of positive physical activity and body fat levels. Second, examining the psychological well-being of adolescents, it was observed that positive interpersonal relations, self-acceptance, sense of life purpose, dominance over the environment and individual growth factors were stronger in participating students than in non-participating students, although there were no differences in terms of autonomy. Third, when explaining the psychological well-being of students who participated in yoga training, it was shown that partial influence was exercised on all sub-factors relating to physical self-conceptualization, while on the other hand, when explaining the psychological well-being of non-participating students, partial influence was enacted on sub-factors of physical self-conceptualization excluding physical activity and muscular strength.

피오렌자의 성서 해석학에 관한 연구

조진경(Cho Jin-Kyung) 한국여신학자협의회 1995 한국여성신학 Vol.- No.23

누가복음서 기자의 여성관

조진경(Cho Jin-Kyung) 한국여신학자협의회 1994 한국여성신학 Vol.- No.17

트레드밀 운동이 당뇨쥐 골격근 및 심근의 p-AMPK, p-ACC 및 Malonyl-CoA 발현에 미치는 영향

조진경(Jin-Kyung Cho),지용석(Yong-Suk Ji),현광석(Kwang-Seok Hyun) 한국생활환경학회 2016 한국생활환경학회지 Vol.23 No.2

This study was to investigated the effects of treadmill exercise on p-AMPK, p-ACC, and Malonyl-CoA expression of skeletal muscle and cardiac muscle in a streptozotocin (STZ)-induced diabetic rats. At 7 weeks of age, S.D rats (n=36) were induced diabetes by administering STZ. A STZ-induced rats were randomly assigned to a control (DM+C, n=12), moderate-intensity exercise group (DM+LE, n=12) and high-intensity exercise group (DM+HE, n=12). The rats were trained progressively on a treadmill, five days per weeks for eight weeks following treadmill running programs: moderate-intensity (15m/min for 60mins) and high-intensity (25m/min for 60mins). After 48-h of the last bout of exercise, blood and tissues of skeletal muscle and heart were harvested. Treadmill running for 8 weeks significantly reduced fasting glucose levels and HOMA-IR in diabetes rat models, regardless of exercise intensity. Both exercise groups significantly increased p-AMPK and p-ACC protein levels. Malonyl-CoA levels were significantly reduced in both exercise groups. The current findings suggest that treadmill running on a STZ-induced diabetes rats can provide an effective means to combat an insulin resistance and improve fatty-acid oxidation (i.e., p-AMPK, p- ACC and malonyl-CoA) in skeletal muscles and heart.

노인의 UCP3유전자 다형과 근감소증 지표 및 골밀도의 관련성

조진경(Jin-Kyung Cho),이인환(In-Hwan Lee),강현식(Hyun-Sik Kang) 한국발육발달학회 2017 한국발육발달학회지 Vol.25 No.2

[Purpose] The purpose of this study was to investigate whether or not the UCP3 polymorphism was associated with sarcopenia and bone mineral density in Korean older adults. [Methods] We assessed two variants of the UCP3 gene in a sample of 296 Korean older adults aged 73.4±6.6 years. Body mass index (BMI), appendicular skeletal muscle mass (ASM) index and bone mineral density (BMD) were analyzed with a dual-energy X-ray absorptiometry (DXA). Hand grip and gait speed were measured with a standardized protocol. One-way ANOVA was used to compare group differences in measured variables across the UCP3 genotypes. [Results] Two variants (rs15763 C/T and rs1800849 C/T) of the UCP3 gene were in the Hardy-Weinberg equilibrium. We found that mean values in hand grip, ASM index and gait speed were not significantly different across the UCP3 rs15763 C/T and UCP3 rs1800849 C/T variants. Lumber and femur BMD were not significantly different according to the UCP3 genotypes. [Conclusion] The current findings suggest that the UCP3 genotypes were not significantly associated with the risks for sarcopenia and bone mineral density in Korean older adults.

Identification of NMR Data for Diol-saponins of Panax ginseng, Ginsenosides Rb₁, Rb₂, Rc, and Rd

Jin-Gyeong Cho(조진경),Jae-Woong Lee,Min-Kyung Lee,Hee-Jeong Park,Dae-Young Lee,Youn-Hyung Lee,Deok-Chun Yang,Nam-In Baek 고려인삼학회 2009 고려인삼학회 학술대회 Vol.2009 No.12