축산식품중의 중금속 함량에 관한 조사연구

정지헌 ( Ji Hun Jung ),황래홍 ( Lae Hwong Hwang ),윤은선 ( En Sun Yun ),김현정 ( Hyun Jung Kim ),한인규 ( In Kyou Han ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.1

This study was performed to investigate the contents of the heavy metals such as cadmium(Cd), copper(Cu), iron(Fe), lead(Pb) and zinc(Zn) in meat and meat products. For this experiment, each 20 samples of beef and pork were collected at slaughterhouse in Seoul areas and 45 samples of meat products(ham, sausage, processed meat and seasoned meat) were purchased from commercial food markets. The contents of the heavy metals was determined by atomic absorption spectrophotometry from Jan. to Nov. in 1998. The results obtained were summarized as follows 1. In Korean native beef, mean values of the heavy metals(mg/kg) were Pb: 0.14±0.10, Cd: 0.07±0.02, Cu: 1.73±0.95, Zn: 42.82±8.17 and Fe: 67.43±24.18, respectively. 2. In imported beef, mean values of the heavy metals(mg/kg) were Pb: 0.03±0.08, Cd: 0.06±0.02, Cu: 1.41±0.62, Zn: 42.93± 4.95 and Fe: 40.06± 12.77, respectively. 3. In pork, mean values of the heavy metals(mg/kg) were Pb: not detected, Cd: 0.09±0.02, Cu: 1.08±0.23, Zn: 19.49±6.02 and Fe: 30.21±10.51, respectively. 4. In ham, mean values of the heavy metals(mg/kg) were Pb: trace, Cd: trace, Cu:1.19± 0.46, Zn: 13.58±4.99 and Fe: 31.43±11.74, respectively. 5. In sausage, mean values of the heavy metals(mg/kg) were Pb: 0.15±0.34, Cd: 0.08±0.05, Cu: 1.15±0.46, Zn: 13.70±7.13 and Fe: 27.28±15.05, respectively. 6. In processed meat, mean values of the heavy metals(mg/kg) were Pb: 0.08±0.13, Cd: 0.09±0.04, Cu: 1.28±0.60, Zn: 14.72±7.09 and Fe: 24.04±10.33, respectively. 7. In seasoned meat, mean values of the heavy metals(mg/kg) were Pb:1.05±2.21, Cd: 0.13 ±0.03, Cu: 1.06± 0.16, Zn: 17.53± 8.72 and Fe: 26.76± 9.02, respectively.

서울시내 수계시설에서 분리된 Legionella spp.의 병원성에 대한 분자역학적 연관성

김진아,정지헌,김수진,진영희,오영희,한기영,Kim, Jin-Ah,Jung, Ji-Hun,Kim, Soo-Jin,Jin, Young-Hee,Oh, Young-Hee,Han, Gi-Young 한국미생물학회 2009 미생물학회지 Vol.45 No.2

Legionella spp. is the causative agent of Legionellosis, which induces a potentially fatal form of pneumonia. With a concentrated performance during the summer of 2008, we secured 73 isolates from the water systems of 25 wards in Seoul. We analysed serotypes, pathogenic genes (Dot/Icm), and patterns of pulsed-field gel electrophoresis (PFGE) in an attempt to confirm relationships among them. Different from the previous year's pattern (2007), among the isolates, 69 were Legionella pneumophila and 4 were Legionella spp. The serotype distribution of Legionella pneumophila was sg1 43, sg6 9, sg5 8, sg3 8, and sg2 1. The serotype for the 4 Legionella spp. was Legionella nautarum. Most of the Legionella pneumophila had several pathogenic genes. On the other hand, the 4 Legionella spp. were defective in pathogenicity in genomic terms. The PFGE patterns of the serotypes showed a tendency for diversity of Legionella pneumophila and a close correlation with genetic pathogenicity. 레지오넬라균은 치명적인 폐렴을 일으킬 수 있는 균으로서, 기후 온난화와 함께 노출의 수위가 높아지고 있는 병원성균이다. 본 연구는 2008년 6월부터 8월까지 서울시내 25개구 소재 수계시설에서 분리한 레지오넬라균 73개 주에 대해 혈청형, Dot/Icm라 일컬어지는 병원성 유전자 분석 그리고 Pulsed field gel electrophoresis (PFGE)를 실시하여 유전자형과 그들 사이의 상관관계를 파악하였다. 73개주 중 69주는 Legionella pneumophila로 혈청형의 분포는 sg1 43주, sg6 9주, sg5 8주, sg3 8주, sg2 1주로 파악되었으며, Legionella spp. 4개주는 Legionella nautarum였다. 분리된 Legionella pneumophila 대부분이 여러 개의 병원성 유전자를 보유하고 있었으며, 반면에 Legionella nautarum은 병원성 유전자가 많이 결핍되어있었다. PFGE pattern을 분석해 볼 때, Legionella pneumophila가 동일한 혈청형안에서 다양하게 분화되어감을 볼 수 있었으며, 병원성 유전자의 분포와 깊은 상관관계를 보였다.

서울시 수계시설에서 분리된 Legionella pneumophila의 분자역학적 특성

전수진,정지헌,승현정,김창규,진영희,오영희,최성민,채영주,Jeon, Su Jin,Jung, Ji Hun,Seung, Hyun Jung,Kim, Chang Kyu,Jin, Young Hee,Oh, Young Hee,Choi, Sung Min,Chae, Young Zoo 한국환경보건학회 2013 한국환경보건학회지 Vol.39 No.2

Objectives: The genus Legionella is common in aquatic environments. Some species of Legionella are recognized as potential opportunistic pathogens for human, notably Legionella pneumophila that causes, Legionellosis. Thus, we investigated the contamination of Legionella pneumophila on water supply systems in Seoul, including cooling towers, public baths, hospitals and fountains. Methods: The existence of 16S rRNA and mip gene of L. pneumophila was confirmed in the genome of the isolated strains by PCR. Results: During the summer season of 2010 and 2011, Legionella pneumophila were detected from 163 samples (21.1%) out of 772 samples collected. Among the 163 strains of L. pneumophila, eighty one isolates belonged to serogroup 1 (57.4%), 23 isolates were serogroup 5 (16.3%), 21 isolates were serogroup 6 (14.9%), 8 isolates were serogroup 2 (5.79%), and 8 isolates were identified in serogroup 3 (5.7%). Through PFGE (pulsed-field gel electrophoresis) analysis using Sfi I, genetic types of L. pneumophila were classified into five (A to E) patterns by the band similarity with excess of 70% from public baths. Conclusions: The PFGE patterns of the serotypes showed a tendency for diversity of L. pneumophila. Our results suggest the existence of serological and genetic diversity among the L. pneumophila isolates.

서울지역 소아에서 분리된 Nontyphoid Salmonella의 항생제 내성과 Integron의 특징

진영희,김진아,정지헌,전수진,이재규,오영희,한기영,이영기,Jin, Young-Hee,Kim, Jin-Ah,Jung, Ji-Hun,Jeon, Soo-Jin,Lee, Jae-Kyoo,Oh, Young-Hee,Han, Ki-Young,Lee, Young-Ki 한국미생물학회 2010 미생물학회지 Vol.46 No.4

2003년부터 2009년까지 서울지역 소아에서 분리된 nontyphoid Salmonella 105주에 대해서 혈청형, 항생제 내성양상, integron의 특징과 PFGE를 수행하였다. 혈청형은 총 18종으로 S.Enteritidis가 가장 많이 분리되었고, 그 다음은 Montevide였다. 항균제 내성은 혈청형별로 차이가 있었으나 전체 살모넬라에 대해서 10종의 항균제에 대한 내성률은 ampicillin이 60%로 가장 높았으며, tetracycline 46.7%, streptomycin 35.2%, nalidixic acid 28.6% 순이었다. 다재내성 유형을 알아본 결과 nalidixic acid 단독 내성이 15.7%로 가장 많았고, ampicillinampicillin/sulbactam-tetracycline형이 14.5%, ampicillin-streptomycin-chloramphenicol-tetracycline형이 10.8%였다. Integron에 대한 연구 결과 integron 보유율은 19%로 20주에서 class 1 integron을 가지고 있었고 gene cassette는 20%만 확인이 되었다. 확인된 gene cassette는 aadA2, blaP1과 dfrA12-aadA2, dfr17-aadA5, aadA7이였다. 연도별 분리균의 유연관계를 확인 하고자 가장 분리율이 높은 S. Enteritidis 50주에 대해서 PFGE를 수행한 결과 3가지 Pulsotype으로 나눠어졌다. 3주를 제외한 모든 균주는 similarity 89.8%의 비교적 유연관계가 높은 균임을 확인할 수 있었다. A total of 105 nontyphoid Salmonella isolated from infants in Seoul from 2003 to 2009 was investigated for their serotype, antimicrobial resistance, characterization of integron, and the patterns of Pulsed-field gel electrophoresis (PFGE). Eighteen serotypes were detected in 105 isolates, and the two most common serotypes were S. Enteritidis (47.6%) and Montevideo (15.2%). Among the Salmonella serovars, a high level of antimicrobial resistance was found to ampicilin (60%), tetracycline (46.7%), streptomycin (35.2%) and nalidixic acid (28.6%). In the multi-drug resistance patterns, the predominant patterns were only nalidixic acid (15.7%), ampicillin-ampicillin/sulbactam-tetracycline (14.5%), and ampicillin-streptomycin-chloramphenicol-tetracycline (10.8%). PCR and DNA sequencing analysis revealed the presence of class 1 integron in 20 isolates (19%). Of the class 1 integron positive isolates 20% harboured the integron-associated gene cassettes : aadA2, blaP1, dfr17-aadA5, dfrA12-aadA2, and aadA7. PFGE was carried out to examine the genetic relatedness among S. Enteritidis isolates. Except for three strains, fifty strains were divided by three pulsotypes.

인공신경망과 SA 알고리즘을 이용한 지능형 생산정 위치 최적화 전산 모델 개발

곽태성(Tae-Sung Kwak),정지헌(Ji-Hun Jung),한동권(Dong-Kwon Han),권순일(Sun-Il Kwon) 한국가스학회 2015 한국가스학회지 Vol.19 No.1

본 연구에서는 고속의 연산이 가능한 인공신경망 시뮬레이터와 SA 알고리즘을 결합하여 지능형 생산정 위치 최적화 전산 모델을 개발하였다. 기존의 사용하는 상용시뮬레이터의 경우 현장 규모의 저류 전산 시뮬레이션을 수행시 시간이 많이 소모되므로 이를 해결하기 위하여 이 모델에서는 인공신경망을 사용하여 짧은 시간 내에 시뮬레이션을 수행할 수 있도록 하였다. 이렇게 얻은 결과를 주관적인 경험에 의거하지 않고 자동으로 최적의 생산정 위치를 선정할 수 있도록 최적화기법인 SA 알고리즘을 적용하였다. 개발된 모델을 사용하여 얻은 결과를 기존 사용 시뮬레이터와 비교하여 예측성능이 양호함을 검증할 수 있었으며, 연산속도 또한 향상됨을 확인하였다. 특히 SA 최적화 알고리즘의 제어변수인 초기온도와 냉각률에 대한 민감도분석을 실시하여 각각에 대한 최적값을 산출하였으며, 이를 통해 개발한 모델의 연산성능을 향상시킬 수 있었다. 마지막으로 개발된 모델을 사용하여 생산정 위치 최적화를 수행한 결과, 생산성이 우수한 지역을 선정하여 최적의 생산정 위치를 도출하였다. This study presents the development of a well placement optimization model, combining an artificial neural network, which enables high-speed calculation, with a simulated annealing algorithm. The conventional FDM simulator takes excessive time when used to perform a field scale reservoir simulation. In order to solve this problem, an artificial neural network was applied to the model to allow the simulation to be executed within a short time. Also by using the given result, the optimization method, SA algorithm, was implemented to automatically select the optimal location without taking any subjective experiences into consideration. By comparing the result of the developed model with the eclipse simulator, it was found that the prediction performance of the developed model has become favorable, and the speed of calculation performance has also been improved. Especially, the optimum value was estimated by performing a sensitivity analysis for the cooling rate and the initial temperature, which is the control parameter of SA algorithm. From this result, it was verified that the calculation performance has been improved, as well. Lastly, an optimization for the well placement was performed using the model, and it concluded the optimized place for the well by selecting regions with great productivity.

생산자료 분석기법을 이용한 셰일가스정 생산거동 연구

이선민(Sun-Min Lee),정지헌(Ji-Hun Jung),신창훈(Chang-Hoon Sin),권순일(Sun-Il Kwon) 한국가스학회 2013 한국가스학회지 Vol.17 No.4

본 연구에서는 캐나다 셰일가스전에 위치한 2개의 생산정에 대해 생산특성에 따라 적절한 생산자료 분석기법을 이용하여 분석을 수행하였다. Case A 생산정의 경우 생산자료가 매우 가변적으로 나타나 시간과 중첩시간을 적용하여 비교분석을 실시하였다. 유동영역을 구분하기 위해 생산자료를 로그-로그 그래프에 도시한 결과 천이 유동구간만 나타났다. 시간과 중첩시간을 적용하여 자극을 받은 저류층 면적이 각각 180, 240 acres로 산출되었고, 원시가스부존량은 15, 20 Bscf로 계산되었다. 그러나 산출된 저류층 면적은 경계영향유동자료로부터 산출된 것이 아니기 때문에 최소 값으로 판단된다. 이에 저류층 면적과 감퇴지수에 대한 생산성 예측을 수행하였다. 그 결과 감퇴지수가 0.5, 1로 커질수록 궁극가채량이 1.2배와 1.4배로 증가하였다. 또한 저류층 면적이 240에서 360 acres로 커지면 궁극가채량이 1.3배 증가되는 것을 확인할 수 있었다. Case B의 고압 저류층에 위치한 생산정은 상부지층압에 따른 지층압축률과 투과도를 적용하여 분석하였다. 지역학적 영향을 적용한 경우와 아닌 경우를 비교한 결과, 저류층 면적은 1.4배, 원시가스부존량이 1.5배로 증가하였다. 셰일 가스전 현장자료에 대한 분석 결과, 분석 방법에 따라 원시가스부존량, 궁극가채량 등 향후 생산성 예측이 크게 달라지므로 생산자료에 따라 유사시간, 중첩시간, 지역학적 분석 등의 적절한 분석방법을 적용하여야 정확한 생산자료 분석이 가능할 것으로 판단된다. This paper presents production data analysis for two production wells located in the shale gas field, Canada, with the proper analysis method according to each production performance characteristics. In the case A production well, the analysis was performed by applying both time and superposition time because the production history has high variation. Firstly, the flow regimes were classified with a log-log plot, and as a result, only the transient flow was appeared. Then the area of simulated reservoir volume (SRV) analyzed based on flowing material balance plot was calculated to 180 acres of time, and 240 acres of superposition time. And the original gas in place (OGIP) also was estimated to 15, 20 Bscf, respectively. However, as the area of SRV was not analyzed with the boundary dominated flow data, it was regarded as the minimum one. Therefore, the production forecasting was conducted according to variation of b exponent and the area of SRV. As a result, estimated ultimate recovery (EUR) increased 1.2 and 1.4 times respectively depending on b exponent, which was 0.5 and 1. In addition, as the area of SRV increased from 240 to 360 acres, EUR increased 1.3 times. In the case B production well, the formation compressibility and permeability depending on the overburden were applied to the analysis of the overpressured reservoir. In comparison of the case that applied geomechanical factors and the case that did not, the area of SRV was increased 1.4 times, OGIP was increased 1.5 times respectively. As a result of analysis, the prediction of future productivity including OGIP and EUR may be quite different depending on the analysis method. Thus, it was found that proper analysis methods, such as pseudo-time, superposition time, geomechanical factors, need to be applied depending on the production data to gain accurate results.

도축돈에서 분리된 살모넬라의 혈청형 및 유전형

최원종 ( Won Zong Choi ),정지헌 ( Ji Hun Jung ),원호근 ( Ho Keun Won ),강정무 ( Zheng Wu Kang ),한태욱 ( Tae Wook Hahn ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.1

Salmonella infections cause the disease in pigs but also some zoonotic Salmonella sero-types can be transmitted to human through swine products, resulting in food poisoning. The objective of this study was to investigate the bacteriological prevalence and detec-tion of invA gene using Salmonella specific polymerase chain reaction(PCR), the epidemio-logical characteristics related to Salmonella strains cultured from pig samples in Gang-won areas using serotyping, random amplified polymorphic DNA(RAPD) and pulsed-field gel electrophoresis(PFGE) methods. During the period of November 2001 through April 2002, 1,174 ileocecal lymph node were collected from the slaughtered pigs raised in 38 farms located in Gangwon province. The samples were submerged in boiling water and macerated in saline and lymph node homogenates were inoculated into Tetrathionate broth with iodine(TTB, Difco, 0.5% iodine was added) for enrichment growth. Then additional tests were performed using several mediums, and suspects were identified by API 20E kit (BioM?rieux) and PCR. Of total 1,174 samples from 38 farms, 44 (3.7%) were isolated as Salmonella spp from 13 farms (34.2%). Of 44 isolates, 31 were in Yangyang region, followed by 9 in Goseong, 2 in both Gangneung and Sokcho. However, there was no difference in regional isolation frequency. All isolates have a 521bp amplified product in Salmonella specific PCR with primer invA which encodes in proteins for invasion of epithelial cells. Of 44 recovered serotypes, 23(52.3%) were S Eingedi, 10(22.7%) S Schwarzengrund, 9(20.5%) S Typhimurium, and 2(4.5%) S Mbandaka. In RAPD analysis, there appeared to be unique bands distin-guishing each serotype, although similarities exist between the different serotypes. Four serotypes of 44 Salmonella isolates appeared to fall into 14 different RAPD types. In PFGE analysis, 9 S Typhimurium were tested with XbaI enzyme and SpeI enzyme. The combination of results obtained with two enzymes subdivided the 9 S Typhimurium into 4 PFGE types.

단보 : 국내산 돼지고기 포장육가공장에서의 serotyping과 PEGE genotyping을 통한 Listeria monocytogenes의 오염 추적

홍종해 ( Chong Hae Hong ),정지헌 ( Ji Hun Jung ) 한국수의공중보건학회 2010 예방수의학회지 Vol.34 No.1

연구논문 : 유통 절임배추의 중금속, 잔류농약 및 식중독 안전성 조사

정선옥 ( Sun Ok Jung ),정지헌 ( Ji Hun Jung ),김성단 ( Sung Dan Kim ),이현경 ( Hyun Yung Lee ),박성규 ( Sung Kyu Park ),이병훈 ( Byung Hun Lee ),이정숙 ( Jeong Sook Lee ),김수진 ( Soo Jin Kim ),김동규 ( Dong Gyu Kim ),유인실 ( In 한국식생활문화학회 2015 韓國食生活文化學會誌 Vol.30 No.6

In recent years, consumers have become more interested in convenient lifestyles, leading to increased use of salted cabbages for preparation of kimchi. This study aimed to investigate the safety of heavy metals, pesticide residues, and foodborne pathogens in commercial salted cabbages in Seoul from August to November, 2014. The survey, which was conducted to determine whether or not salted cabbages were prepared under the highest sanitary conditions, showed that Seoulites are interested in purchasing hygienic and safe salted cabbages. The average amounts (range) of Pb and Cd found in 30 salted cabbage samples were 0.007 (0.000~0.063) mg/kg and 0.004 (0.000~0.012) mg/kg, respectively. The cabbages were analyzed for residues from 285 types of pesticides using the multiresidue method. Residues for pesticides were not detected. Major foodborne pathogens, specifically Escherichia coli, Salmonella spp., Listeria monocytogenes, Campylobacter jejuni, Staphylococcus aureus, Vibrio parahaemolyticus, Enterohemorrhagic Escherichia coli, Bacillus cereus, Clostridium perfringens and Norovirus, were also not detected.

도라지(Platycodon grandiflorum A. De Candolle) 섭취에 따른 알루미늄의 노출량 평가

김성단(Sung-Dan Kim),함희진(Hee-Jin Ham),정지헌(Ji-Hun Jung),이은순(Eun-Soon Lee),이현경(Hyun-Kyung Lee),김희선(Hee-Sun Kim),이집호(Jib-Ho Lee),유인실(In-Sil Yu),정권(Kweon Jung) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.8

2014~2015년 서울시내 대형마트, 소형마트 및 재래시장 등에서 유통 중인 천연 및 단순가공 도라지 총 93건(깐도라지 53건, 피도라지 40건)의 알루미늄 잔류량을 분석한 결과 평균 및 95th percentile 알루미늄 잔류량은 72.989(0.54~564.38) mg/kg 및 471.32 mg/kg이었다. 깐도라지 53건의 평균 및 95th percentile 알루미늄 잔류량은 121.235(0.54~564.38) mg/kg 및 545.622 mg/kg이었으며, 피도라지 40건의 평균 및 95th percentile 알루미늄 잔류량은 9.063(0.72~28.05) mg/kg 및 26.379 mg/kg이었다. 단순가공 깐도라지의 평균 알루미늄 잔류량은 천연 상태 피도라지에 비해 13.4배 높은 수준으로 유의적인 차이가 있었다(P<0.001). 도라지의 알루미늄 분석값과 제6기 1차 연도(2013년)의 국민건강영양조사 중 영양조사(조사1일 전 식품섭취 내용, 24시간 회상법)의 도라지 섭취량 평균값, 최소값, 최대값 및 95th percentile 값을 이용하여 단일값 분석을 실시하여 추정식이섭취량(estimated daily intake, EDI)을 산출한 결과 영양조사 대상자 전체(시나리오Ⅰ)의 도라지 섭취를 통한 평균 알루미늄 EDI는 0.001(~1.363) mg/kg bw/d였으며, 섭취자군(시나리오Ⅱ)의 평균 및 95th percentile EDI는 각각 0.033(0.000~1.309) 및 0.610 mg/kg bw/d였다. 도라지 섭취량을 연중 일상섭취량으로 가정하여 추정주간식이섭취량(estimated weekly intake, EWI)을 산출하여 잠정주간섭취허용량(provisional tolerable weekly intake, PTWI)과 비교하여 평가한 결과 섭취자군(시나리오 Ⅱ)의 평균 추정주간식이섭취량은 0.231 mg/kg bw/week(23.1% PTWI)였다. This study was performed to estimate daily intakes of aluminum from Platycodon grandiflorum A. De Candolle and evaluate their potential health risks for Koreans. Estimated daily intake of aluminum was calculated, whereas the actual level of aluminum in Platycodon grandiflorum A. De Candolle was analyzed using inductively coupled plasma mass spectrometry. Food consumption amount was drawn from Korea National Health and Nutrition Examination Survey (KNHANES Ⅵ-1). In analysed samples, aluminum values ranged from 0.54∼564.38 mg/kg in peeled Platycodon grandiflorum A. De Candolle samples (n=53) and from 0.72∼28.05 mg/kg in unpeeled ones (n=40). Statistically significant difference (P<0.001) was detected according to the type of skin. To estimate the dietary intake of Platycodon grandiflorum A. De Candolle, a total of 7,242 respondents (scenario Ⅰ) were compared to 227 Platycodon grandiflorum A. De Candolle consumption subjects (scenario Ⅱ). Estimated daily intake of aluminum was calculated based on point estimates. Level of safety for aluminum was evaluated by comparison with Provisional Tolerable Weekly Intake (PTWI), 1 mg/kg bw, set by the Joint FAO/WHO Expert Committee on Food Additives. For scenario Ⅰ, mean estimated daily intake of aluminum was 0.001 mg/kg bw/d. For scenario Ⅱ, mean estimated daily intake of aluminum was 0.033 mg/kg bw/d, and 95th percentile estimated daily intake was 0.610 mg/kg bw/d. For scenario Ⅱ, aluminum from Platycodon grandiflorum A. De Candolle had a mean weekly intake that was the 23.1% of PTWI.