Prognostic Significance of EPHB2 Expression in Colorectal Cancer Progression

장보근,김혜성,장원영,배정모,강경훈 대한병리학회 2018 Journal of Pathology and Translational Medicine Vol.52 No.5

Background: A receptor tyrosine kinase for ephrin ligands, EPHB2, is expressed in normal colorectal tissues and colorectal cancers (CRCs). The aim of this study was to investigate EPHB2 expression over CRC progression and determine its prognostic significance in CRC. Methods: To measure EPHB2 mRNA and protein expression, real-time polymerase chain reaction and immunohistochemistry were performed in 32 fresh-frozen and 567 formalin-fixed paraffin-embedded CRC samples, respectively. We further investigated clinicopathological features and overall and recurrence-free survival according to EPHB2 protein expression. Results: The EPHB2 level was upregulated in CRC samples compared to non-cancerous tissue in most samples and showed a strong positive correlation with AXIN2. Notably, CD44 had a positive association with both mRNA and protein levels of EPHB2. Immunohistochemical analysis revealed no difference in EPHB2 expression between adenoma and carcinoma areas. Although EPHB2 expression was slightly lower in invasive fronts compared to surface area (p < .05), there was no difference between superficial and metastatic areas. EPHB2 positivity was associated with lymphatic (p < .001) and venous (p = .001) invasion, TNM stage (p < .001), and microsatellite instability (p = .036). Kaplan–Meier analysis demonstrated that CRC patients with EPHB2 positivity showed better clinical outcomes in both overall (p = .049) and recurrence-free survival (p = .015). However, multivariate analysis failed to show that EPHB2 is an independent prognostic marker in CRCs (hazard ratio, 0.692; p = .692). Conclusions: Our results suggest that EPHB2 is overexpressed in a subset of CRCs and is a significant prognostic marker.

우슈선수들의 성취목표성향과 운동몰입 및 스포츠 자신감과의 관계

장보근,문한식 대한무도학회 2022 대한무도학회지 Vol.24 No.4

본 연구는 우슈선수들의 성취목표성향과 운동몰입 및 스포츠 자신감과의 관계를 검증하는 데 목적을 두었다. 최종분석은 361부가 사용되었고 AMOS 25.0과 SPSS 25.0 프로그램을 통해 확인적 요인분석, 빈도분석, 신뢰도 분석, 상관관계분석, 다중회귀분석을 실시한 결과는 다음과 같다. 첫째, 우슈선수들의 과제지향과 자아지향이 인지몰입에 긍정적인 영향을 미쳤고, 과제지향이 행위몰입에도 긍정적인영향을 미쳤다. 둘째, 우슈선수들의 행위몰입이 투지, 압박감, 여유에 긍정적인 영향을 미쳤고, 행위몰입과 인지몰입이 신체준비에 긍정적인 영향을 미쳤으나, 인지몰입은 압박감과 여유에 부정적인 영향을 미쳤다. 셋째, 우슈선수들의 과제지향은 투지와 신체준비에 긍정적인 영향을 미쳤다. 이상의 결과성취목표성향는 운동몰입과 스포츠 자신감에 관여되고 있으며 운동몰입이 높을수록 스포츠 자신감에도 긍정적인 영향을 미친다는 것을 시사한다.

Expression of BamHI-A Rightward Transcripts in Epstein-Barr Virus-Associated Gastric Cancers

장보근,정은지,김우호 대한암학회 2011 Cancer Research and Treatment Vol.43 No.4

Purpose About 10% of all gastric cancers (GCs) are Epstein-Barr virus (EBV)-associated. However, the oncogene of EBV in gastric carcinogenesis has not yet been established. In the present study,we investigated the virus-derived transcripts in the EBV-infected GC cell line to explore the viral oncogene of EBV-positive GCs. Materials and Methods We used the SNU719 cell line, a naturally derived EBV-infected GC cell line. The individual expressed sequence tags from the cDNA libraries of SNU719 were searched against the mRNA subset extracted from the GenBank data base. Sequence reaction was carried out for the EBVassociated clones. Reverse transcription-polymerase chain reaction was performed after cells were partitioned into nuclear and cytoplasmic fractions. Results Using bioinformatic tools, we selected 13 EBV-associated clones from cDNA libraries of SNU719. By sequencing analysis, we revealed that they were all associated with RPMS1, one of the BamHIA rightward transcripts (BART) of EBV. Some BART cDNAs such as RPMS1 and A73 are known to be translated into protein in vitro, and have been shown to have some biochemical functions relevant to tumorigenesis. But, presently, the BART transcripts were expressed only in the nucleus and not in the cytoplasm, arguing against their role as messenger RNAs. Some other BART transcripts expressed in GCs (BARF0, CST, vIL, BARF1, BLLF1, and BcLF1) were also extensively detected in the nucleus. Conclusion BART transcripts are the predominant viral transcripts expressed in EBV-associated GCs, and they are located only in the nucleus. Therefore, it seems less likely that BART transcripts produce functional proteins to play a role in carcinogenesis of EBV-associated GCs.

풍력발전용 초전도케이블을 위한 초전도 선의 교류손실 분석

장보근(B. G. Jang),박민원(Minwon Park),유인근(I. K. Yu) 한국정밀공학회 2014 한국정밀공학회 학술발표대회 논문집 Vol.2014 No.5월

Development of a PHILS facility for a 154 kV class HTS AC power cable

Bo-Geun Jang(장보근),Sung-Kyu Kim(김성규),Minh-Chau Dinh(딘민차우),Minwon Park(박민원),In-Keun Yu(유인근),Byeongmo Yang(양병모) 대한전기학회 2014 대한전기학회 학술대회 논문집 Vol.2014 No.7

Harmonic Loss Analysis of an HTS DC Power Cable applied to the Hanlim HVDC System

Bo-Geun Jang(장보근),Jong-Ho Choi(최종호),Sung-Kyu Kim(김성규),Jin-Geun Kim(김진근),Minwon Park(박민원),In-Keun Yu(유인근),Byeongmo Yang(양병모),Hyun-Man Jang(장현만),Chang-Yeol Choi(최창열) 대한전기학회 2013 대한전기학회 학술대회 논문집 Vol.2013 No.7

위에 국한된 결정저장조직구증

장희수,송현주,장보근 대한상부위장관ㆍ헬리코박터학회 2016 Korean Journal of Helicobacter Upper Gastrointesti Vol.16 No.2

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Elevated expression of Axin2 in intestinal metaplasia and gastric cancers

이동휘,정인호,장보근 대한병리학회 2023 Journal of Pathology and Translational Medicine Vol.57 No.6

Background: The Wnt signaling pathway regulates crucial cellular processes, including stem cell development and tissue repair. Dysregulation of this pathway, particularly β-catenin stabilization, is linked to colorectal carcinoma and other tumors. Axin2, a critical component in the pathway, plays a role in β-catenin regulation. This study examines Axin2 expression in normal gastric mucosa and various gastric pathologies. Methods: Formalin-fixed and paraffin-embedded tissue samples from normal stomach, gastritis, intestinal metaplasia (IM), and gastric carcinoma were collected. Axin2 and β-catenin expression were evaluated using RNA in situ hybridization and immunohistochemistry, respectively. Histo-scores (H-scores) were calculated to quantify expression levels of Axin2. Associations between Axin2 expression and clinicopathological variables were examined. Results: Axin2 expression was examined in normal stomach, gastritis, and IM tissues. Axin2 expression was mainly observed in the surface and isthmus areas in the normal stomach and gastritis, whereas Axin2 expression was markedly higher at the bases of IM. Axin2 H-scores were significantly elevated in IM (mean ± standard deviation [SD], 87.0 ± 38.9) compared to normal (mean ± SD, 18.0 ± 4.5) and gastritis tissues (mean ± SD, 33.0 ± 18.6). In total, 30% of gastric carcinomas showed higher Axin2 expression. Axin2 expression did not have significant associations with age, sex, Lauren classification, histological differentiation, invasion depth, and lymph node metastasis. However, a strong positive correlation was observed between Axin2 and nuclear β-catenin in gastric carcinomas (p < .001). Conclusions: Axin2 expression was significantly increased in IM compared to normal and gastritis cases. In addition, Axin2 showed a strong positive association with nuclear β-catenin expression in gastric carcinomas, demonstrating a close relationship with abnormal Wnt/β-catenin signaling pathway.

별세포종에서 CD44와 MMP14의 발현과 WHO 등급과의 연관성 및 이들이 환자의 생존에 미치는 영향 연구

박성혜,명재경,장보근,박혜성,윤웅재,이현주 대한병리학회 2010 Journal of Pathology and Translational Medicine Vol.44 No.1

Background : CD44 is a cell surface receptor that has been implicated in tumor cell invasion and metastasis in a range of tumors of various organs, including breast, ovary, colon, lung, and brain. CD44 stimulates the invasive ability by interacting with matrix metalloproteinase 14 (MMP14). The expression of MMP14 on the cell surface is thought to trigger multiple proteinase cascades and to stimulate cell migration. Methods : A total 54 astrocytoma patients were eligible for this study. We performed a retrospective clinicopathological review and CD44 and MMP14 immunohistochemistry. Results : The expressions of CD44 and MMP14 were significantly correlated with the World Health Organization (WHO) grade. On univariate analysis, the WHO grade and the expression of CD44 were the significant prognostic factors affecting overall survival (OS) and disease progression free survival (DPFS). On the multivariate analysis by the Cox regression model, the only WHO grade was shown to be a significant independent prognostic factor for predicting the DPFS and OS. Conclusions : In this study, the CD44 and MMP14 expressions were related to the WHO grade of astrocytoma. The CD44 expression status was a prognostic factor for DPFS and OS on univariate analysis, but it was not an independent prognostic factor on the multivariate analysis.

Aurora Kinase A Is a Prognostic Marker in Colorectal Adenocarcinoma

고현민,맹영희,장보근,현창림,김영실,현진원,장원영 대한병리학회 2017 Journal of Pathology and Translational Medicine Vol.51 No.1

ackground: Aurora kinase A (AURKA), or STK15/BTAK, is a member of the serine/threonine kinase family and plays important roles in mitosis and chromosome stability. This study investigated the clinical significance of AURKA expression in colorectal cancer patients in Korea. Methods: AURKA protein expression was evaluated by immunohistochemistry in 151 patients with colorectal adenocarcinoma using tissue microarray blocks. We analyzed the relationship between clinicopathological characteristics and AURKA expression. In addition, the prognostic significance of various clinicopathological data for progression-free survival (PFS) was assessed. Also we evaluated copy number variations by array comparative genomic hybridization and AURKA gene amplification using fluorescence in situ hybridization in colorectal carcinoma tissues. Results: AURKA gene amplification was found more frequently in the 20q13.2–13.33 gain-positive group than the group with no significant gain on the AURKA-containing locus. AURKA protein expression was detected in 45% of the cases (68/151). Positive staining for AURKA was observed more often in male patients (p = .035) and distally located tumors (p = .021). PFS was shorter in patients with AURKA expression compared to those with low-level AURKA expression (p < .001). Univariate analysis revealed that AURKA expression (p = .001), age (p = .034), lymphatic invasion (p = .001), perineural invasion (p = .002), and TNM stage (p = .013) significantly affected PFS. In a multivariate analysis of PFS, a Cox proportional hazard model confirmed that AURKA expression was an independent and significant prognostic factor in colorectal adenocarcinoma (hazard ratio, 3.944; p < .001). Conclusions: AURKA could serve as an independent factor to