Production of γ-Aminobutyric Acid Using Immobilized Glutamate Decarboxylase from Lactobacillus plantarum

이상재,이한승,이동우,Lee, Sang-Jae,Lee, Han-Seung,Lee, Dong-Woo The Korean Society for Microbiology and Biotechnol 2015 한국미생물·생명공학회지 Vol.43 No.3

효율적인 γ-aminobutyric acid (GABA)의 생산을 위해 Lactobacillus plantarum WCFS1로부터 글루탐산 탈탄산효소(glutamate decarboxylase, GAD)를 대장균에 발현, 정제 후 silica beads에 covalent coupling 방법을 이용하여 고정화하였다. 고정화된 효소의 특성을 고정화하지 않은 효소와 비교한 결과, 모든 pH의 범위(pH 3.5–6.0)에서 80% 이상의 활성을 나타내었으며 pH 안정성과 열 안정성 모두 증대되었다. 이 고정화 효소를 packed-bed reactor에 충진하여 GABA의 생산성을 확인한 결과 1리터당 1시간에 최대 41.7 g의 GABA 생산이 가능한 것으로 확인되었다. The glutamate decarboxylase gene (gadB) from Lactobacillus plantarum WCFS1 was cloned and expressed as an N-terminal hexa-histidine-tagged fusion protein in Escherichia coli BL21 (DE3) as the host strain. Purified glutamate decarboxylase (GAD) was immobilized onto porous silica beads by covalent coupling. The pH dependence of activity and stability of the immobilized GAD was significantly altered, when compared to those of the free enzyme. Immobilized GAD was stable in the range of pH 3.5 to 6.0. The resulting packed-bed reactor produced 41.7 g of γ-aminobutyric acid/l·h at 45℃.

저전력 8-비트 마이크로콘트롤러의 설계

이상재,정항근,Lee, Sang-Jae,Jeong, Hang-Geun 대한전자공학회 2002 電子工學會論文誌-SD (Semiconductor and devices) Vol.39 No.2

본 논문에서는 저전력 8-비트 RISC 마이크로콘트롤러 구조를 제안하였다. 설계된 마이크로콘트롤러는 4단계 파이프라인 구조를 가지며 기존의 여러 가지 저전력 설계 기법들을 이용하여 구현되었다. 전력 소모는 0.6㎛ 공정을 사용했을 때 MIPS당 600㎼를 소모했으며 0.25㎛ 공정을 사용했을 때 MIPS당 70㎼를 소모했다. RTL 레벨의 설계는 VHDL을 이용해서 수행되었고, 0.6㎛/0.2㎛ CMOS IDEC(Integrated Circuit Design Education Center) standard cell library를 이용해서 게이트 레벨에서 기능 검증을 하였다. 합성된 코어는 0.25㎛ 공정을 용했을 때 약 7000개의 NAND 게이트를 0.36㎟의 작은 면적에 집적화 시킬 수 있었다. 마지막으로 기존의 상용 마이크로콘트롤러와의 성능 비교를 수행하였다. This paper suggests a 8-bit RISC microcontroller, which has a 4-stage pipeline architecture. Many low-power design techniques that have been proposed by previous works are adopted into it. The proposed microcontroller consumes only 600㎼ per MIPS for 0.6 ${\mu}{\textrm}{m}$ CMOS process and even lower power of 70㎼ per MIPS for 0.25${\mu}{\textrm}{m}$ process. The RTL level design of this microcontroller is carried out using VHDL. The functional verification is thoroughly done at the gate level using 0.6${\mu}{\textrm}{m}$/0.25${\mu}{\textrm}{m}$ CMOS IDEC standard cell library. This microcontroller contains 7000 NAND gates on a 0.36$\textrm{mm}^2$ die using 0.25${\mu}{\textrm}{m}$ process. Finally the comparison of power consumption with other conventional microcontrollers is provided.

"조선왕조실록"을 통해 본 한국의 차문화

이상재,정지훈,Lee, Sangjae,Jung, Jihun 대한예방한의학회 2013 대한예방한의학회지 Vol.17 No.2

According to "The Annals of the Joseon Dynasty", we know that korean ancestors confuse tea prescription with green tea. It makes cultural misunderstanding Tea doesn't mean green tea but tea prescription. It is caused that tea prescription has been called tea habitually in Korea. Korean don't drink green tea much. Instead, they have had a habit of drinking decoction of medicinal herbs and they called it tea. This habit has developed into Korean traditional tea culture. In the palace in the Joseon Dynasty period, Ginseng tea was used in the tea ceremony. When the king had poor health or had a disease or was even on the eve of death, a royal physician tried to cure him by tea. They used about 30 kinds of tea according to "The Annals of the Joseon Dynasty" Such as ginseng tea, astragalus tea, ginger tea. Tea in korea is not a drink but the way to cure a disease. Korean people usually get to drink decoction of other herbs instead of green tea. This became the basis of korean traditional tea culture. We need to change a definition of Korean tea like this way and study about it much more. With this research paper, korean tea culture should be studied on more various ways and established itself as original and unique tea culture. On the basis of this studies, Korean tea brand can be famous in the world like Chinese tea or Japanese tea.

Colour Concrete의 강도변화에 따른 연구

이상재,Lee, Sang-Jae 한국레미콘공업협회 1989 레미콘 Vol.3 No.-

수출 클레임

이상재,Lee, Sang-Jae (사)한국포장협회 2007 包裝界 Vol.171 No.-

Mu-Negative Metamaterial 단일 셀을 가진 듀얼 대역 모노폴 안테나 설계

이상재,이영훈,Lee, Sang-Jae,Lee, Young-Hun 한국전기전자학회 2017 전기전자학회논문지 Vol.21 No.3

This paper was studied the double-band monopole antenna design with Mu-negative metamaterial unit cell, which operates at 700MHz and 2.45GHz band. Mu-negative unit cell made of the interdigital capacitor structure to operate a double-band antenna by inserting it into an antenna radiator unit. In addition, the parasitic conductor is implemented on the back side of the antenna radiation part, so that the resonance point of the antenna can be controlled and the bandwidth is improved. Finally, we implemented an antenna operating in the 750MHz UHD band and the 2.45GHz WiFi band. The designed antenna has a size of $200{\times}100mm^2$. Experimental results show that the 8dB bandwidth and gain characteristics at 750MHz band are 320MHz(42.7%), 5.28dB, 6dB bandwidth and gain at 2.45GH are 540MHz (21.6%), -0.46dB. From the experimental results, we confirmed that the resonance point with theoretical value is in agreement with experimental value, and the radiation patterns are have the omnidirectional characteristic in both bands. 본 논문에서는 Mu-negative Metamaterial 단일 셀을 사용하여 700MHz대역과 2.45GHz 대역에서 동작하는 이중대역 모노폴 안테나 설계에 관하여 연구하였다. Mu-negative metamaterial 단일셀은 interdigital capacitor 구조를 사용하였고, 안테나 방사부에 삽입함으로써 이중대역 안테나를 구현하였다. 또한 기생도체를 안테나 방사부 뒷면에 있도록 구현하여 안테나의 공진점을 제어할 수 있고, 대역폭을 개선하였다. 최종적으로 750MHz UHD대역과 2.45GHz WiFi 대역에서 동작하는 안테나를 구현하였다. 설계제작한 안테나의 크기는 $200{\times}100mm^2$이고, 실험 결과 750MHz 대역에서 8dB 대역폭과 이득특성은 각각 320MHz(42.8%), 5.28dB, 2.45GHz에서 6dB 대역폭과 이득은 540MHz(21.6%), -0.46dB이다. 공진점은 이론값과 실험값이 일치하였으며, 방사패턴은 두 대역에서 전방향 특성을 갖고 있음을 확인하였다.

${\ll}$준생팔전${\gg}$에 기재된 진희이이십사절기좌공도인법(陳希夷二十四節氣坐功導引法)에 대한 연구 I - 춘계양생(春季養生) -

이상재,이송실,김광호,Lee Sang-Jae,Lee Song-Shil,Kim Kwang-Ho 대한예방한의학회 2001 대한예방한의학회지 Vol.5 No.2

Jinhee-Twentyfour method Qigong (陳希夷二十四節氣坐功導引) is a traditional exercise which is known as qi-gong. It was first mentioned in ${\ll}$保生心鑒${\gg}$ published in Myung dynasty. But it is very difficult to know how to master Jinhee-Twentyfour method Qigong (陳希夷二十四節氣坐功導引) So we studied the training methods by analizing several books such as ${\ll}$遵生八${\gg}$ and ${\ll}$內外功圖說輯要${\gg}$ which have pictures of the training methods. It is known that to train Jinhee-Twentyfour method Qigong (陳希夷二十四節氣坐功導引) is not only limited to the exercise of the body but also training of the mind is an important factor. It is very important to know that the system of Hyung(形), Qi(氣), Sin(神) is needed for Jinhee-Twentyfour method Qigong (陳希夷二十四節氣坐功導引)

철도역사 안전관리 자동화 기술 개발

이상재,김동현,안세영,Lee, Sang-Jae,Kim, Dong-Hyeon,An, Se-Yeong 대한교통학회 2016 교통 기술과 정책 Vol.13 No.6

특집 - 수출 포장산업 현황

이상재,Lee, Sang-Jae (사)한국포장협회 2009 包裝界 Vol.197 No.-

체내이식 무선기기용 디지털 모뎀의 설계 및 구현

이상재,김태민,Lee, Sang-Jae,Kim, Tae-Min 한국전기전자학회 2012 전기전자학회논문지 Vol.16 No.1

본 논문에서는 체내 이식 무선기기용 디지털 모뎀을 국제표준화에 맞는 주파수 402~405Mhz 대역에서 작동하는 저출력 무선시스템으로 설계하고 구현하였다. 저전력 모뎀 ${\pi}$/4-D8PSSK는 전력 효율적인 변복조 방식으로 저전력 특성이 우수할 뿐만 아니라, 대역 효율적인 변복조 방식으로 PSK의 단점을 극복할 수 있다. 설계된 디지털 모뎀은 Matlab 시뮬레이션 파형과 Chipscope를 이용하여 측정한 파형 비교를 통하여 검증하고 FPGA로 구현하였다. In this paper, a digital modem for the medical implant communication system will operate in 402~405 Mhz wide-band in international standardization frequency was designed and implemented. The ${\pi}$/4-D8PSSK modem, which is not only power effective with a good low-powered characteristics but also band-width effective, can overcome the shortcoming of PSK. The proposed digital modem was verified through the wave comparison based on the result of Matlab simulation and the wave from the measured one using Chipscope, and implemented with FPGA.